scholarly journals The gut microbiota in the common kestrel (Falco tinnunculus): a report from the Beijing Raptor Rescue Center

PeerJ ◽  
2020 ◽  
Vol 8 ◽  
pp. e9970
Author(s):  
Yu Guan ◽  
Hongfang Wang ◽  
Yinan Gong ◽  
Jianping Ge ◽  
Lei Bao
Keyword(s):  
Gut Microbiota ◽  
Ribosomal Rna ◽  
Animal Species ◽  
High Throughput Sequencing ◽  
Body Structure ◽  
Falco Tinnunculus ◽  
Sequencing Technology ◽  
Rescue Center ◽  
The Common ◽  
Protected Animal

As a complex microecological system, the gut microbiota plays crucial roles in many aspects, including immunology, physiology and development. The specific function and mechanism of the gut microbiota in birds are distinct due to their body structure, physiological attributes and life history. Data on the gut microbiota of the common kestrel, a second-class protected animal species in China, are currently scarce. With high-throughput sequencing technology, we characterized the bacterial community of the gut from nine fecal samples from a wounded common kestrel by sequencing the V3-V4 region of the 16S ribosomal RNA gene. Our results showed that Proteobacteria (41.078%), Firmicutes (40.923%) and Actinobacteria (11.191%) were the most predominant phyla. Lactobacillus (20.563%) was the most dominant genus, followed by Escherichia-Shigella (17.588%) and Acinetobacter (5.956%). Our results would offer fundamental data and direction for the wildlife rescue.

scholarly journals The Microbiome in Primary Sclerosing Cholangitis: Current Evidence and Potential Concepts

2017 ◽  
Vol 37 (04) ◽  
pp. 314-331 ◽  
Author(s):  
Johannes Hov ◽  
Tom Karlsen
Keyword(s):  
Gut Microbiota ◽  
Primary Sclerosing Cholangitis ◽  
Sclerosing Cholangitis ◽  
High Throughput Sequencing ◽  
Current Evidence ◽  
Published Data ◽  
Sequencing Technology ◽  
Close Relationship ◽  
Inflammatory Bowel ◽  
Host Metabolism

AbstractThe close relationship between primary sclerosing cholangitis (PSC) and inflammatory bowel disease has inspired hypothetical models in which gut bacteria or bacterial products are key players in PSC pathogenesis. Several studies using high-throughput sequencing technology to characterize the gut microbiota in PSC have been published over the past years. They all report reduced diversity and significant shifts in the overall composition of the gut microbiota. However, it remains unclear as to whether the observed changes are primary or secondary to PSC development and further studies are needed to assess the biological implications of the findings. In the present article, we review the published data in perspective of similar studies in other diseases. We discuss aspects of methodology and study design that are relevant to interpretation of the data. Furthermore, we propose that interpretation and further assessments of findings are structured into conceptual compartments, and elaborate three such possible concepts relating to immune function (the “immunobiome”), host metabolism (the “endobiome”), and dietary and xenobiotic factors (the “xenobiome”) in PSC.

scholarly journals Comparative Analysis of the Gut Microbial Communities of the Eurasian Kestrel (Falco tinnunculus) at Different Developmental Stages

2020 ◽  
Vol 11 ◽  
Author(s):  
Lei Zhou ◽  
Xiaona Huo ◽  
Boyu Liu ◽  
Hui Wu ◽  
Jiang Feng
Keyword(s):  
Gut Microbiota ◽  
Microbial Communities ◽  
High Throughput Sequencing ◽  
Research Work ◽  
Wild Birds ◽  
Growth Stages ◽  
Rrna Gene ◽  
Gut Microflora ◽  
Falco Tinnunculus ◽  
Eurasian Kestrel

The gut microflora play a very important role in the life of animals. Although an increasing number of studies have investigated the gut microbiota of birds in recent years, there is a lack of research work on the gut microbiota of wild birds, especially carnivorous raptors, which are thought to be pathogen vectors. There are also a lack of studies focused on the dynamics of the gut microbiota during development in raptors. In this study, 16S rRNA gene amplicon high-throughput sequencing was used to analyze the gut microbiota community composition of a medium-sized raptor, the Eurasian Kestrel (Falco tinnunculus), and to reveal stage-specific signatures in the gut microbiota of nestlings during the pre-fledging period. Moreover, differences in the gut microbiota between adults and nestlings in the same habitat were explored. The results indicated that the Eurasian Kestrel hosts a diverse assemblage of gut microbiota. Proteobacteria, Firmicutes, Actinobacteria, and Bacteroidetes were the primary phyla shared within the guts of adults and chicks. However, adults harbored higher abundances of Proteobacteria while nestlings exhibited higher abundances of Firmicutes and Actinobacteria, and consequently the majority of dominant genera observed in chicks differed from those in adults. Although no significant differences in diversity were observed across the age groups during nestling ontogeny, chicks from all growth stages harbored richer and more diverse bacterial communities than adults. In contrast, the differences in gut microbial communities between adults and younger nestlings were more pronounced. The gut microbes of the nestlings in the last growth stage were converged with those of the adults. This study provides basic reference data for investigations of the gut microbiota community structure of wild birds and deepens our understanding of the dynamics of the gut microflora during raptor development.

scholarly journals Safety Evaluation of Neo Transgenic Pigs by Studying Changes in Gut Microbiota Using High-Throughput Sequencing Technology

PLoS ONE ◽  
2016 ◽  
Vol 11 (3) ◽  
pp. e0150937 ◽  
Author(s):  
Qingqing Wang ◽  
Lili Qian ◽  
Shengwang Jiang ◽  
Chunbo Cai ◽  
Dezun Ma ◽  
...  
Keyword(s):  
Gut Microbiota ◽  
High Throughput ◽  
High Throughput Sequencing ◽  
Safety Evaluation ◽  
Sequencing Technology ◽  
Transgenic Pigs

scholarly journals Effect of Pit Mud on Bacterial Community and Aroma Components in Yellow Water and Their Changes during the Fermentation of Chinese Strong-Flavor Liquor

Foods ◽  
2020 ◽  
Vol 9 (3) ◽  
pp. 372 ◽  
Author(s):  
Zhanzheng Gao ◽  
Zhengyun Wu ◽  
Wenxue Zhang
Keyword(s):  
High Throughput Sequencing ◽  
Microbial Population ◽  
Solid Phase ◽  
Gas Chromatography Mass Spectrometry ◽  
Sequencing Technology ◽  
Fermentation Time ◽  
Flavor Components ◽  
Pit Mud ◽  
The Common ◽  
Aroma Components

As the main by-product of Chinese strong-flavor liquor, yellow water plays an important role in the formation of flavor components. Yellow water from different fermentation periods (30th day, 45th day, 60th day) was selected to analyze the aroma components by Headspace solid phase micro-extraction Gas Chromatography–Mass Spectrometry, and the microorganism community was evaluated by high-throughput sequencing technology and bioinformatics analysis of DNA. As the fermentation time was prolonged, the main flavor components significantly increased, and the amount of the common microbial population between yellow water and pit mud increased gradually. Among the common microorganisms, Lactobacillus accounted for the largest proportion, at about 56.96%. The microbes in the yellow water mainly belonged to Firmicutes. The abundance of Bacilli (the main bacteria) gradually decreased with time, at 87.60% at the 30th day down to 68.87% at the 60th day, but Clostridia gradually increased from 10.29% to 27.48%. At the genus level, some microbes increased significantly from the 30th day to 60th day, such as Caproiciproducens, which increased from 2.65% to 6.30%, and Sedimentibacter, increasing from 0.47% to 2.49%. RDA analysis indicated that the main aroma components were positively correlated with Clostridia and negatively correlated with Bacilli.

scholarly journals Integrative Analysis of LGR5/6 Gene Variants, Gut Microbiota Composition and Osteoporosis Risk in Elderly Population

2021 ◽  
Vol 12 ◽  
Author(s):  
Dong-sheng Di ◽  
Can Li ◽  
Yu Dai ◽  
Mu-hong Wei ◽  
Shan-shan Wang ◽  
...  
Keyword(s):  
Gut Microbiota ◽  
High Throughput Sequencing ◽  
Bioinformatic Analysis ◽  
Microbiota Composition ◽  
Chinese Han ◽  
Elderly Chinese ◽  
The Common ◽  
The Relationship ◽  
Osteoporosis Risk ◽  
Gut Microbiota Composition

Objective: This study aimed to explore the relationships between the common variants of R-spondin/Wnt signaling genes, gut microbiota composition, and osteoporosis (OP) risk in elderly Chinese Han population.Design: Dual-energy X-ray absorptiometry was used to obtain the OP-associated measurements at multiple skeleton sites among all 1,168 participants. Genotyping data was obtained by using the next-generation sequencing in the discovery stage (n = 400, 228 OP patients) and SNPscan technology in the replication stage (n = 768, 356 OP patients). Bioinformatic analysis was performed to provide more evidence for the genotype-OP associations. The 16S ribosomal RNA gene high-throughput sequencing technology was adopted to explore OP-associated gut microbiota variations.Results: The genetic variants of rs10920362 in the LGR6 gene (P-FDR = 1.19 × 10–6) and rs11178860 in the LGR5 gene (P-FDR = 1.51 × 10–4) were found to associate with OP risk significantly. Several microbial taxa were associated with the BMDs and T-scores at multiple skeleton sites. The associations between rs10920362 and BMD-associated microbiota maintained significance after adjusting confounders. The rs10920362 CT/TT genotype associated with a decreased relative abundance of Actinobacteria (β = −1.32, P < 0.001), Bifidobacteriaceae (β = −1.70, P < 0.001), and Bifidobacterium (β = −1.70, P < 0.001) compared to the CC genotype.Conclusion: Our findings suggested that the variants loci of LGR6 may be associate with OP pathogenesis via gut microbiota modifications. The relationship between host genetics and gut microbiome provides new perspectives about OP prevention and treatment.

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