Variation in Chlorophyll a and b in the Seagrass Halodule in Central Visayas, Philippines

2014 ◽  
Vol 8 (1) ◽  
Author(s):  
BILLY THEODORUS WAGEY
Keyword(s):  
Chlorophyll Content ◽  
Chlorophyll A ◽  
Photosynthetic Activity ◽  
Light Availability ◽  
Experimental Manipulation ◽  
Marine Ecology ◽  
Chlorophyll A And B ◽  
Photosynthetic Performances ◽  
Plastic Responses ◽  
One Way Anova

Chlorophyll content of seagrass leaves varies among species and habitats byat least a factor of five, and shows plastic responses to seasonal variations in light availability as well as direct experimental manipulation. This study generally attempted to observe the relative amount of chlorophyll a, b content of Haloduleuninervis and Halodule pinifolia in Central Visayas, Philippines in relation tothese environmental factors which are: exposure, density, temperature, and lightintensity. Chlorophyll was determined using the equation of Jeffrey and Humprey(1975) whereas data gathered went through one way ANOVA followed byTukey’s Test. Significant variations were found in most photosynthetic parametersmeasured in this study. Overall, H. uninervis and H. pinifolia seems to obtainoptimal photosynthetic performances over a broad range of irradiances. Mainly,genus Halodule can be seen as a highlight species, which has also the ability to acclimate to low irradiances. in general, the result indicated that seagrasses tend to decrease the ratio of chlorophyll a:b by increasing the amount of chlorophyllb to compensate for the decline in light intensity in subtidal areas. Therefore, H.uninervis and H. pinifolia seem to have the ability of adjusting their chlorophyllcontent in performing optimal photosynthetic activity over a broad range of irradiance regimes.Keywords: Marine Ecology, chlorophyll content, seagrass, Halodule, experimental design,Philippines

scholarly journals Variations of Chlorophyll Content at Abies alba and Nepeta pannonica Species According to Phenophase and Harvesting Area

2019 ◽  
Vol 52 (1) ◽  
pp. 74-78
Author(s):  
S. Buhăianu ◽  
Doina Carmen Jităreanu
Keyword(s):  
Chlorophyll Content ◽  
Chlorophyll A ◽  
Photosynthetic Pigments ◽  
Photosynthetic Activity ◽  
Abies Alba ◽  
Chlorophyll B ◽  
Green Color ◽  
Yellow Coloration ◽  
Chlorophyll A And B ◽  
Chlorophyll A Content

Abstract Chlorophylls from plants are photosynthetic pigments. Their quantity offers valuable informations about photosynthetic activity, growing and developing of plants. Photosynthetic pigments decrease quantitatively during senescence process or in stress conditions. The present study has been realized in laboratory conditions with material harvested from spontaneous flora. The purpose of this research was the investigation of variations of chlorophyll content from samples of biological material collected from Nepeta pannonica L. and Abies alba Mill. plants, from Câmpulung Moldovenesc and Cacica areas, Suceava county, Romania. The targeted phenophases were growth and flowering. There were realized acetonic extracts from samples for spectrophotometric determinations. Obtained data were processed to estabilish chlorophyll a and b content. There were observed that at Abies alba species, from both locations, the chlorophyll a content grew during flowering phenophase, while the chlorophyll b content had little variations. At Nepeta pannonica species, the chlorophyll a and b content decreased visibly during the flowering, due to stress. Leaves of plants from this species presented a intense green color in the growing phenophase, while during flowering phenophase they had a purple or yellow coloration. Obtained results revealed a different dynamics of chlorophyll content at studied species.

scholarly journals Analisis Kandungan Klorofil Pada Anak Daun Tanaman Kelapa (Analysis of Chlorophyll Content in Children Leaves of Coconut Plants)

2019 ◽  
Vol 9 (2) ◽  
pp. 76
Author(s):  
Muhammad Tawary ◽  
Julius Pontoh ◽  
Lydia Momuat
Keyword(s):  
Chlorophyll Content ◽  
Chlorophyll A ◽  
Economic Value ◽  
Economic Benefits ◽  
Chlorophyll B ◽  
Solvent Extraction Method ◽  
Chlorophyll A And B ◽  
The Right ◽  
Almost All ◽  
Chlorophyll A Content

Analisis Kandungan Klorofil Pada Anak Daun Tanaman Kelapa (Analysis of Chlorophyll Content in Children Leaves of Coconut Plants) Muhammad Tawary1*), Julius Pontoh1), Lydia I.Momuat1)1)Jurusan Kimia, FMIPA UNSRAT Manado*Email korespondensi: [email protected] Diterima 7 Juli 2019, diterima untuk dipublikasi 10 Agustus 2019 Abstrak Tanaman kelapa banyak dibudidayakan sebagai tanaman pertanian. Tanaman kelapa memiliki nilai ekonomi yang tinggi karena hampir semua bagian tanaman kelapa memiliki manfaat ekonomis. Penelitian bertujuan untuk  mengembangkan metode analisis klorofil pada daun dan menentukan bagian anak daun yang memiliki kandungan klorofil tertinggi. Analisis kandungan klorofil dilakukan dengan metode ektraksi pelarut yang absorbansinya dibaca pada spektrofotometer UV-Vis. Kandungan klorofil a dalam daun bervariasi pada setiap bagian anak daun tanaman kelapa. Kandungan klorofil a pada posisi tengah anak daun kelapa (763.19 µmol/m2) dan kandungan klorofil b (196.22 µmol/m2). Kandungan klorofil a dan b pada setiap bagian anak daun dan posisi kanan dan kiri anak daun memiliki nilai yang relatif sama.Kata kunci: Kelapa, klorofil a, klorofil b Abstract Many coconut plants are cultivated as agricultural crops. Coconut plants have high economic value because almost all parts of coconut plants have economic benefits. The study aims to develop a method of chlorophyll analysis on leaves and determine the part of leaflets which have the highest chlorophyll content. Analysis of chlorophyll content was carried out by a solvent extraction method in which the absorbance was read in a UV-Vis spectrophotometer. The content of chlorophyll a in leaves varies with each part of the leaves of the coconut plant. Chlorophyll a content in the middle position of coconut leaf child (763.19 µmol / m2) and chlorophyll b content (196.22 µmol / m2). The content of chlorophyll a and b in each part of the leaf child and the right and left positions of the leaves have relatively the same value.Keywords: Coconut, Chlorophyll a, Chlorophyll b

scholarly journals COMPARATIVE CHARACTERISTICS OF PHOTOSYNTHETIC ACTIVITY OF NECTARINE CULTIVARS AND FORMS WITH DIFFERENT COLORED LEAF PLATE

AGROFOR ◽  
2018 ◽  
Vol 2 (1) ◽  
Author(s):  
Iuliia IVASHCHENKO ◽  
Yuriy IVASHCHENKO ◽  
Evgeny SHOFERISTOV ◽  
Sergei TSIUPKA
Keyword(s):  
Powdery Mildew ◽  
Chlorophyll A ◽  
Energy Supply ◽  
Photosynthetic Activity ◽  
Green Color ◽  
Primary Reactions ◽  
Chlorophyll A And B ◽  
Traditional Cultivars ◽  
The Stability ◽  
Kautsky Effect

Genotypes of nectarine with red leaves were created in Nikita botanical gardens.They have commercially valuable properties and biological characteristics (resistanceto powdery mildew, large-fruited, the ability of transmitting the trait of redleaves with constant result to progeny seed, etc. ). The aim of our researches was tostudy the main indicators of photoactivity of leaf apparatus of nectarine with greenand anthocyanin leaves coloration. Investigations have been carried out in 2010-2015 on intact leaf plates for three cultivars and forms of nectarine with greenleaves – Rubinoviy-8 (control), Chemus, Krymtsuht 53-85 and 2 forms withanthocyanin coloration of leaf plate (NektadianaKrasnolistnaya 996-88, Krasnola495-86). Photosynthetic activity was characterized by chlorophyll fluorescenceparameters (Kautsky effect). The content of chlorophyll a and b were determinedby spectrophotometry. The cultivar Rubinoviy-8 and the form - Krasnola 495-86were selected according to intensity indicators of the primary reactions ofphotosynthesis. Anthocyanin forms concede cultivar Rubinoviy-8 in efficiency ofthe primary reactions of photosynthesis an average of 48-50%; but they exceednectarines Chemus and Krymtsuht 53-85 with green color of leaves of 23-25%.The efficiency of energy supply in the "dark" reactions of photosynthesis in allvarieties and forms of nectarine maintained within 41-46%. But the further abilityto utilize received energy is most efficiently implemented at the nectarine cultivarRubinoviy-8 and form Krasnola 495-86. The forms of red-leaved nectarine incomparison with traditional cultivars are distinguished by a great potential forretaining the stability and productivity of the functioning of the photosyntheticapparatus.

Analsis Kandungan Klorofil pada Beberapa Jenis Tanaman Palma (Analysis of Chlorophyll Content in Several Types of Palm Plants)

2019 ◽  
Vol 9 (1) ◽  
pp. 44
Author(s):  
Muhammad Tawary ◽  
Julius Pontoh ◽  
Lydia Momuat
Keyword(s):  
Chlorophyll Content ◽  
Chlorophyll A ◽  
Oil Palm ◽  
Coconut Oil ◽  
Chlorophyll B ◽  
Agricultural Crops ◽  
Solvent Extraction Method ◽  
Chlorophyll A And B ◽  
The Right ◽  
Chlorophyll A Content

Analsis Kandungan Klorofil pada Beberapa Jenis Tanaman Palma(Analysis of Chlorophyll Content in Several Types of Palm Plants) Muhammad Tawary1)*, Julius Pontoh1), Lydia Momuat1)1)Jurusan Kimia, FMIPA Universitas Sam Ratulangi,  Manado 95115*Email korespondensi: [email protected] Diterima  15 Februari 2019, diterima untuk dipublikasi 28 Februari  2019 Abstrak Tanaman palma banyak dibudidayakan sebagai tanaman pertanian. Ada beberapa jenis tanaman palma seperti kelapa, kelapa sawit, dan aren. Penelitian ini bertujuan untuk mengembangkan metode analisis klorofil pada daun dan menentukan posisi anak daun yang memiliki kandungan klorofil tertinggi. Analisis kandungan klorofil dilakukan dengan metode ektraksi pelarut yang absorbansinya dibaca pada spektrofotometer UV-Vis. Kandungan klorofil a dalam daun bervariasi pada ketiga jenis tanaman palma, yaitu pada daun kelapa berkisar antara 586.56 sampai 835.75 µmol/m2, daun kelapa sawit 674.47 µmol/m2 sampai 982.98 µmol/m2, dan pada daun aren 1300.82 µmol/m2 sampai 1449.65 µmol/m2. Klorofil b pada daun kelapa berkisar antara 160.79 µmol/m2 sampai 237.86 µmol/m2, daun kelapa sawit 191.36 µmol/m2 sampai 315.63 µmol/m2, dan daun aren 423.29 sampai 543.19 µmol/m2. Kandungan klorofil a pada setiap posisi anak daun ketiga jenis tanaman palma memiliki nilai yang hampir sama sedangkan kandungan klorofil b pada setiap posisi anak daun pada ketiga jenis tanaman palma memiliki nilai yang sedikit berbeda. Kandungan klorofil a dan b pada setiap bagian anak daun aren memiliki nilai yang hampir sama begitupun dengan kelapa dan kelapa sawit. Kandungan klorofil a pada posisi kanan dan kiri daun aren memiliki nilai yang sedikit berbeda sedangkan pada kelapa dan kelapa sawit memiliki nilai yang hampir sama. Kandungan klorofil b pada ketiga jenis tanaman palma tidak memiliki perbedaan.Kata kunci: Palma, klorofil, absorbansi, spektrofometer Abstract Many palma plants are cultivated as agricultural crops. Several types of palm plants, for example coconut, oil palm, and sugar palm. This research was conducted to develop a method of chlorophyll analysis on leaves and determine the position of leaves children who have the highest chlorophyll content. Analysis of chlorophyll content was carried out by a solvent extraction method in which the absorbance was read in a UV-Vis spectrophotometer. TheThe content of chlorophyll contained in coconut leaves varies, namely containing chlorophyll content contained in coconuts varies, which contains 586.56 µmol/m2 to 835.75 μmol / m2, in oil palm leaves 674.47 µmol/m2 to 982.98 μmol / m2, and in palm leaves 1300.82 to 1449.65 μmol / m2. Whereas for chlorophyll b found in coconut leaves varies around 160.79 µmol/m2 to 237.86 µmol / m2, on palm oil leaves 191.36 µmol/m2 to 315.63 µmol / m2, and on palm leaves 423.29 µmol/m2  to 543.19 µmol / m2. The content of chlorophyll a at each position of leaflets on leaves of various types of palma plants has almost the same value while the content of chlorophyll b at each position of leaflets on the leaves of various types of palma plants has slightly different values. The content of chlorophyll a and b in each part of palm leaves has almost the same value as well as coconut and oil palm. Chlorophyll a content in the right and left position of palm leaves has slightly different values while coconut and oil palm have almost the same value. Chlorophyll b content in all three types of palma plants has no difference.Keywords: Palma, chlorophyll, absorbance, spectrophotometer

scholarly journals Seasonal changes of photosynthetical parameters as a results of forest gap model

2015 ◽  
pp. 133-136
Author(s):  
Árpád Szalacsi ◽  
Gergely Király ◽  
Szilvia Veres
Keyword(s):  
Chlorophyll Content ◽  
Chlorophyll A ◽  
Light Stress ◽  
High Light ◽  
Forest Area ◽  
Plant Production ◽  
Photosynthetic Parameters ◽  
Total Chlorophyll ◽  
Total Chlorophyll Content ◽  
Chlorophyll A And B

Photosynthetic parameters of English oak (Quercus robur L.) as a member of Querco robori-Carpinetum were investigated in two different habitat in terms of gap forest management: in the gap and in the host forest. The artifical opening process of the forest resulted in more light for growing saplings and need for acclimatization. Photosynthesis is one of the most important way for plant life and plant production. In the centre of photosynthetic efficiency the quality and quantity traits of photosynthetic pigments are standing. During our work some photosynthetic parameters of plants (in the gap and in the forest as well) were measured: relative chlorophyll content as SPAD index, chlorophyll a and b content, total chlorophyll content and ratio of chlorophyll a and b. Based on our results no significant differences among our data in early spring. Although, during the summer significant differences occurred between the measured values in the gap and in the forest area. Lower total chlorophyll content was experienced in the gap, than in the forest area due to the lower chlorophyll-b content. Because of the high light intensity higher chla/chlb ratio was measured in the gap. The lower chlorophyll contents of gap habitat may have a part of the acclimatization process of photosynthetic apparatus against high light stress, which can determinate the survival chance of individual.

scholarly journals ANALISIS KANDUNGAN KLOROFIL PADA BERBAGAI POSISI DAUN DAN ANAK DAUN AREN (Arrenga pinnata)

2020 ◽  
Vol 12 (2) ◽  
Author(s):  
Olivia P. G. Lawendatu ◽  
Julius Pontoh ◽  
Vanda Kamu
Keyword(s):  
Chlorophyll Content ◽  
Chlorophyll A ◽  
Palm Tree ◽  
Plant Canopy ◽  
Chlorophyll B ◽  
Chlorophyll A And B ◽  
Palm Leaf

ABSTRAKTelah dilakukan penelitian tentang analisis kandungan klorofil dari berbagai posisi daun dan anak daun dari tanaman aren. Daun aren diambil pada posisi atas, tengah, dan bawah dari tajuk tanaman. Sampel anak daun  dibagi 3 posisi yaitu atas, tengah, dan bawah. Ekstraksi dilakukan dengan methanol dan penetuan klorofil pada daun dilakukan dengan spektofotometer pada panjang gelombang 665 nm dan 652 nm untuk masing-masing klorofil a dan klorofil b. Kandungan klorofil a dan b  yang terdapat pada sampel daun aren yang dihitung berdasarkan 3 unit satuan µg/mL, mg/g  dan µmol/m2. Kandungan klorofil a dan b pada sampel pohon aren tertinggi terletak pada posisi kiri daun, pada posisi daun terletak pada daun atas, pada posisi anak daun terletak pada posisi ujung anak daun. Ratio kandungan klorofil pada sampel daun aren berkisar 1,30-6,72. ABSTRACTHas conducted research on the analysis of chlorophyll content from various positions of leaves and leaves of palm plants. Leaves are taken in the top, middle and bottom positions of the plant canopy. Samples of leaflets were divided into 3 positions namely top, middle and bottom. Extraction was carried out with methanol and the determination of chlorophyll in the leaves was carried out by spectrophotometer at wavelengths of 665 nm and 652 nm for chlorophyll a and chlorophyll b, respectively. The chlorophyll and b content in palm leaf samples are calculated based on 3 units of μg/mL, mg/g, and µmol/m2. The content of chlorophyll a and b in the highest palm tree sample is located in the left position of the leaf, the position of the leaf is placed on the top of the leaf, the position of the leaflets is placed in the tip of the leaflets. The ratio of chlorophyll content in sugar palm leaf samples in the setting is 1.30-6.72. 

scholarly journals Changes of respiration intensity and chlo'rophyll content in needles of Normay spruce (Picea abies L. Karst) seedlings treated with 2,4,5-T and dalapon

2015 ◽  
Vol 28 (2) ◽  
pp. 177-195 ◽  
Author(s):  
Jan Tonecki
Keyword(s):  
Acetic Acid ◽  
Picea Abies ◽  
Chlorophyll Content ◽  
Chlorophyll A ◽  
Sodium Salt ◽  
Butyl Ester ◽  
Bud Formation ◽  
Bud Development ◽  
Chlorophyll A And B ◽  
Stimulation Of

The results of investigations on the effect of 2,4,5-T (butyl ester of 2,4,5-trichlorophenoxy acetic acid) and dalapon (sodium salt of 2,2-dichloropropionic acid) on chlorophyll content and rate of respiration in needles of spruce seedlings, treated with different concentrations of both herbicides before bud development and during bud formation' are presented. 2,4,5-T and dalapon decreased chlorophyll a and b content in the needles. The chlorophyll a : b ratio increased after treatment with these herbicides. The lower concentrations tested produced a higher value of this coefficient. Depending on concentration both herbicides caused disturbances in respiration of needles of seedlings treated during bud formation. Stimulation of respiration by both herbicides in the newly formed needles of seedlings treated before bud development was observed.

scholarly journals Productivity of triticale depending on the content of photosynthesizing pigments at anthesis

2021 ◽  
Vol 12 (2) ◽  
pp. 15-22
Author(s):  
B.O. Mazurenko ◽  
Keyword(s):  
Chlorophyll Content ◽  
Chlorophyll A ◽  
Photosynthetic Pigments ◽  
Dry Matter ◽  
Nitrogen Fertilizers ◽  
Chlorophyll B ◽  
Varietal Response ◽  
Chlorophyll A And B ◽  
Fertilizer System

Photosynthetic pigments play an important role in the accumulation of dry matter and they can be the markers of stress. Change in the ratio of chlorophyll A and B indicates physiological changes and adaptation of the organism to changes of environmental conditions. Sowing terms and application of nitrogen fertilizers are long-term factors, so the content of chlorophylls in the flag leaf indicates long-term adaptation of the photosynthetic system. Establishing a relationship between the chlorophyll content at anthesis and the accumulation of dry matter in posy-anthesis period indicates the varietal response of triticale to fertilization and sowing dates. It was conducted a three-factor field experiment. There are studied two winter and one facultative triticale cultivar, two autumn sowing terms and fertilization system with application the different rates of nitrogen fertilizers in different growth stages. It was found that the content of photosynthetic pigments in each variety varied depending on sowing term and fertilizer system in terms of mg per 1 g of dry matter for chlorophyll A, B and the amount of A+B. At the same time, the difference between the factors and their interactions was insignificant for the total chlorophyll mass per unit area (g/m2) for chlorophyll A and the amount of A+B, but it was significant for chlorophyll B by the fertilizer system factor. This indicates that the main stress signal is chlorophyll B, so the ratio of chlorophyll A and B differed significantly depending on this factor. Cla: Clb ratio in the optimal sowing period is 5.3–8.1 in cv. Pidzimok kharkivskiy, 4.8–8.3 in cv. Amur and 5.0–6.7 in cv. Obriy mironivskiy. All cultivars have a strong positive correlation between the chlorophyll content at anthesis and accumulation of dry matter at post-anthesis perion under optimal conditions, but facultative triticale Pidzimok kharkivskiy has a negative correlation with unfavorable, due to increased chlorophyll, without increasing dry matter accumulation. Further study of the relationship between chlorophyll content and the synthesis of primary and secondary metabolites is promising in the study of varietal response to stress and agronomic factors.

scholarly journals Morphology and pigment content of sporophytes and gametophytes of ten fern species

2019 ◽  
Vol 48 (4) ◽  
pp. 951-956
Author(s):  
Asia Bibi ◽  
Shabbir Ejaz ◽  
Sidra Rafique ◽  
Saima Ashraf
Keyword(s):  
Chlorophyll Content ◽  
Chlorophyll A ◽  
Morphological Study ◽  
Pigment Content ◽  
Carotenoid Content ◽  
Anthocyanin Content ◽  
Pigment Contents ◽  
Fern Species ◽  
Chlorophyll A And B

The morphology and pigment contents of sporophytes and gametophytes of ten fern species were studied. In the morphological study, variations were found in stipe, texture, rachis, fronds and sori of these species. Photosynthestic pigment contents of sporophyte and gametophyte of ten species vary to a great extent. Even sporophyte and gametophyte of same species vary in pigment content. Sporophyte revealed higher chlorophyll content than gametophyte. The highest chlorophyll a and b were found in the gametophyte of Cetarach officinarum Gametophyte of Adiantum flabellatum produced maximum anthocyanin whereas minimum anthocyanin content was found in the sporophyte of Cetarach dalhousiae. The highest carotenoid content was obtained from the gametophyte of Cheilanthus albomarginata. Altitudinal variations were also found to affect morphology and pigment content of sporophytes and gametophytes of ten fern species.

scholarly journals The role of light in the adaptation of Thymus praecox Opiz subsp. praecox for diverse habitat conditions

2020 ◽  
Vol 48 (3) ◽  
pp. 1194-1209
Author(s):  
Beata BARABASZ-KRASNY ◽  
Katarzyna MOŻDŻEŃ ◽  
Anna SOŁTYS-LELEK ◽  
Ingrid TURISOVÁ
Keyword(s):  
Chlorophyll A ◽  
Cell Membranes ◽  
Physiological Activity ◽  
Light Availability ◽  
Dry Mass ◽  
Habitat Conditions ◽  
Partial Shade ◽  
Chlorophyll A And B ◽  
Role Of Light

The light decides about the course and efficiency of photochemical processes, being an important component of the surrounding environment shaping the plant composition in specific conditions. Thymus praecox subsp. praecox belongs to endangered taxa due to preferences for open habitats, which as a result of natural succession are exposed to overgrowth and shading with forest-scrub vegetation. In this study, an attempt was made to check the physiological activity of creeping thyme in changing environmental conditions prevailing on isolated stands in the Ojców National Park (Southern Poland). The increase in fresh and dry mass and the percentage of water in plants were determined, the content of chlorophyll a and b was measured, the intensity of chlorophyll a fluorescence was examined and the degree of electrolytes leakage through cell membranes was checked. The main aim was to determining the optimal habitat conditions for this taxon, which could help protect it. Based on the conducted research it was found, among others higher mass increase in plants from a sunny stand. Regardless of the measurement period, an increase in chlorophyll a and b content and a higher degree of cell membranes destabilisation in plants from a partially shaded stand was observed. The obtained results show, that T. praecox subsp. praecox characterises small habitat flexibility - even partial shade is not a convenient habitat for it. To optimal development, this subspecies requires conditions with high light availability. Creeping thyme is a heliophilic and thermophilic taxa and the sunny stands are optimal for it.

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