scholarly journals Variations of Chlorophyll Content at Abies alba and Nepeta pannonica Species According to Phenophase and Harvesting Area

2019 ◽  
Vol 52 (1) ◽  
pp. 74-78
Author(s):  
S. Buhăianu ◽  
Doina Carmen Jităreanu
Keyword(s):  
Chlorophyll Content ◽  
Chlorophyll A ◽  
Photosynthetic Pigments ◽  
Photosynthetic Activity ◽  
Abies Alba ◽  
Chlorophyll B ◽  
Green Color ◽  
Yellow Coloration ◽  
Chlorophyll A And B ◽  
Chlorophyll A Content

Abstract Chlorophylls from plants are photosynthetic pigments. Their quantity offers valuable informations about photosynthetic activity, growing and developing of plants. Photosynthetic pigments decrease quantitatively during senescence process or in stress conditions. The present study has been realized in laboratory conditions with material harvested from spontaneous flora. The purpose of this research was the investigation of variations of chlorophyll content from samples of biological material collected from Nepeta pannonica L. and Abies alba Mill. plants, from Câmpulung Moldovenesc and Cacica areas, Suceava county, Romania. The targeted phenophases were growth and flowering. There were realized acetonic extracts from samples for spectrophotometric determinations. Obtained data were processed to estabilish chlorophyll a and b content. There were observed that at Abies alba species, from both locations, the chlorophyll a content grew during flowering phenophase, while the chlorophyll b content had little variations. At Nepeta pannonica species, the chlorophyll a and b content decreased visibly during the flowering, due to stress. Leaves of plants from this species presented a intense green color in the growing phenophase, while during flowering phenophase they had a purple or yellow coloration. Obtained results revealed a different dynamics of chlorophyll content at studied species.

scholarly journals Analisis Kandungan Klorofil Pada Anak Daun Tanaman Kelapa (Analysis of Chlorophyll Content in Children Leaves of Coconut Plants)

2019 ◽  
Vol 9 (2) ◽  
pp. 76
Author(s):  
Muhammad Tawary ◽  
Julius Pontoh ◽  
Lydia Momuat
Keyword(s):  
Chlorophyll Content ◽  
Chlorophyll A ◽  
Economic Value ◽  
Economic Benefits ◽  
Chlorophyll B ◽  
Solvent Extraction Method ◽  
Chlorophyll A And B ◽  
The Right ◽  
Almost All ◽  
Chlorophyll A Content

Analisis Kandungan Klorofil Pada Anak Daun Tanaman Kelapa (Analysis of Chlorophyll Content in Children Leaves of Coconut Plants) Muhammad Tawary1*), Julius Pontoh1), Lydia I.Momuat1)1)Jurusan Kimia, FMIPA UNSRAT Manado*Email korespondensi: [email protected] Diterima 7 Juli 2019, diterima untuk dipublikasi 10 Agustus 2019 Abstrak Tanaman kelapa banyak dibudidayakan sebagai tanaman pertanian. Tanaman kelapa memiliki nilai ekonomi yang tinggi karena hampir semua bagian tanaman kelapa memiliki manfaat ekonomis. Penelitian bertujuan untuk  mengembangkan metode analisis klorofil pada daun dan menentukan bagian anak daun yang memiliki kandungan klorofil tertinggi. Analisis kandungan klorofil dilakukan dengan metode ektraksi pelarut yang absorbansinya dibaca pada spektrofotometer UV-Vis. Kandungan klorofil a dalam daun bervariasi pada setiap bagian anak daun tanaman kelapa. Kandungan klorofil a pada posisi tengah anak daun kelapa (763.19 µmol/m2) dan kandungan klorofil b (196.22 µmol/m2). Kandungan klorofil a dan b pada setiap bagian anak daun dan posisi kanan dan kiri anak daun memiliki nilai yang relatif sama.Kata kunci: Kelapa, klorofil a, klorofil b Abstract Many coconut plants are cultivated as agricultural crops. Coconut plants have high economic value because almost all parts of coconut plants have economic benefits. The study aims to develop a method of chlorophyll analysis on leaves and determine the part of leaflets which have the highest chlorophyll content. Analysis of chlorophyll content was carried out by a solvent extraction method in which the absorbance was read in a UV-Vis spectrophotometer. The content of chlorophyll a in leaves varies with each part of the leaves of the coconut plant. Chlorophyll a content in the middle position of coconut leaf child (763.19 µmol / m2) and chlorophyll b content (196.22 µmol / m2). The content of chlorophyll a and b in each part of the leaf child and the right and left positions of the leaves have relatively the same value.Keywords: Coconut, Chlorophyll a, Chlorophyll b

Analsis Kandungan Klorofil pada Beberapa Jenis Tanaman Palma (Analysis of Chlorophyll Content in Several Types of Palm Plants)

2019 ◽  
Vol 9 (1) ◽  
pp. 44
Author(s):  
Muhammad Tawary ◽  
Julius Pontoh ◽  
Lydia Momuat
Keyword(s):  
Chlorophyll Content ◽  
Chlorophyll A ◽  
Oil Palm ◽  
Coconut Oil ◽  
Chlorophyll B ◽  
Agricultural Crops ◽  
Solvent Extraction Method ◽  
Chlorophyll A And B ◽  
The Right ◽  
Chlorophyll A Content

Analsis Kandungan Klorofil pada Beberapa Jenis Tanaman Palma(Analysis of Chlorophyll Content in Several Types of Palm Plants) Muhammad Tawary1)*, Julius Pontoh1), Lydia Momuat1)1)Jurusan Kimia, FMIPA Universitas Sam Ratulangi,  Manado 95115*Email korespondensi: [email protected] Diterima  15 Februari 2019, diterima untuk dipublikasi 28 Februari  2019 Abstrak Tanaman palma banyak dibudidayakan sebagai tanaman pertanian. Ada beberapa jenis tanaman palma seperti kelapa, kelapa sawit, dan aren. Penelitian ini bertujuan untuk mengembangkan metode analisis klorofil pada daun dan menentukan posisi anak daun yang memiliki kandungan klorofil tertinggi. Analisis kandungan klorofil dilakukan dengan metode ektraksi pelarut yang absorbansinya dibaca pada spektrofotometer UV-Vis. Kandungan klorofil a dalam daun bervariasi pada ketiga jenis tanaman palma, yaitu pada daun kelapa berkisar antara 586.56 sampai 835.75 µmol/m2, daun kelapa sawit 674.47 µmol/m2 sampai 982.98 µmol/m2, dan pada daun aren 1300.82 µmol/m2 sampai 1449.65 µmol/m2. Klorofil b pada daun kelapa berkisar antara 160.79 µmol/m2 sampai 237.86 µmol/m2, daun kelapa sawit 191.36 µmol/m2 sampai 315.63 µmol/m2, dan daun aren 423.29 sampai 543.19 µmol/m2. Kandungan klorofil a pada setiap posisi anak daun ketiga jenis tanaman palma memiliki nilai yang hampir sama sedangkan kandungan klorofil b pada setiap posisi anak daun pada ketiga jenis tanaman palma memiliki nilai yang sedikit berbeda. Kandungan klorofil a dan b pada setiap bagian anak daun aren memiliki nilai yang hampir sama begitupun dengan kelapa dan kelapa sawit. Kandungan klorofil a pada posisi kanan dan kiri daun aren memiliki nilai yang sedikit berbeda sedangkan pada kelapa dan kelapa sawit memiliki nilai yang hampir sama. Kandungan klorofil b pada ketiga jenis tanaman palma tidak memiliki perbedaan.Kata kunci: Palma, klorofil, absorbansi, spektrofometer Abstract Many palma plants are cultivated as agricultural crops. Several types of palm plants, for example coconut, oil palm, and sugar palm. This research was conducted to develop a method of chlorophyll analysis on leaves and determine the position of leaves children who have the highest chlorophyll content. Analysis of chlorophyll content was carried out by a solvent extraction method in which the absorbance was read in a UV-Vis spectrophotometer. TheThe content of chlorophyll contained in coconut leaves varies, namely containing chlorophyll content contained in coconuts varies, which contains 586.56 µmol/m2 to 835.75 μmol / m2, in oil palm leaves 674.47 µmol/m2 to 982.98 μmol / m2, and in palm leaves 1300.82 to 1449.65 μmol / m2. Whereas for chlorophyll b found in coconut leaves varies around 160.79 µmol/m2 to 237.86 µmol / m2, on palm oil leaves 191.36 µmol/m2 to 315.63 µmol / m2, and on palm leaves 423.29 µmol/m2  to 543.19 µmol / m2. The content of chlorophyll a at each position of leaflets on leaves of various types of palma plants has almost the same value while the content of chlorophyll b at each position of leaflets on the leaves of various types of palma plants has slightly different values. The content of chlorophyll a and b in each part of palm leaves has almost the same value as well as coconut and oil palm. Chlorophyll a content in the right and left position of palm leaves has slightly different values while coconut and oil palm have almost the same value. Chlorophyll b content in all three types of palma plants has no difference.Keywords: Palma, chlorophyll, absorbance, spectrophotometer

scholarly journals Productivity of triticale depending on the content of photosynthesizing pigments at anthesis

2021 ◽  
Vol 12 (2) ◽  
pp. 15-22
Author(s):  
B.O. Mazurenko ◽  
Keyword(s):  
Chlorophyll Content ◽  
Chlorophyll A ◽  
Photosynthetic Pigments ◽  
Dry Matter ◽  
Nitrogen Fertilizers ◽  
Chlorophyll B ◽  
Varietal Response ◽  
Chlorophyll A And B ◽  
Fertilizer System

Photosynthetic pigments play an important role in the accumulation of dry matter and they can be the markers of stress. Change in the ratio of chlorophyll A and B indicates physiological changes and adaptation of the organism to changes of environmental conditions. Sowing terms and application of nitrogen fertilizers are long-term factors, so the content of chlorophylls in the flag leaf indicates long-term adaptation of the photosynthetic system. Establishing a relationship between the chlorophyll content at anthesis and the accumulation of dry matter in posy-anthesis period indicates the varietal response of triticale to fertilization and sowing dates. It was conducted a three-factor field experiment. There are studied two winter and one facultative triticale cultivar, two autumn sowing terms and fertilization system with application the different rates of nitrogen fertilizers in different growth stages. It was found that the content of photosynthetic pigments in each variety varied depending on sowing term and fertilizer system in terms of mg per 1 g of dry matter for chlorophyll A, B and the amount of A+B. At the same time, the difference between the factors and their interactions was insignificant for the total chlorophyll mass per unit area (g/m2) for chlorophyll A and the amount of A+B, but it was significant for chlorophyll B by the fertilizer system factor. This indicates that the main stress signal is chlorophyll B, so the ratio of chlorophyll A and B differed significantly depending on this factor. Cla: Clb ratio in the optimal sowing period is 5.3–8.1 in cv. Pidzimok kharkivskiy, 4.8–8.3 in cv. Amur and 5.0–6.7 in cv. Obriy mironivskiy. All cultivars have a strong positive correlation between the chlorophyll content at anthesis and accumulation of dry matter at post-anthesis perion under optimal conditions, but facultative triticale Pidzimok kharkivskiy has a negative correlation with unfavorable, due to increased chlorophyll, without increasing dry matter accumulation. Further study of the relationship between chlorophyll content and the synthesis of primary and secondary metabolites is promising in the study of varietal response to stress and agronomic factors.

Effects of Phosphorus Stress on Photosynthetic Pigments in Sagittaria sagittifolia

2014 ◽  
Vol 1073-1076 ◽  
pp. 118-121
Author(s):  
Qing Qing He ◽  
Zhou Li Liu ◽  
Wei Chen ◽  
Lin Hao ◽  
Jiao Jiao Qin ◽  
...  
Keyword(s):  
Chlorophyll Content ◽  
Chlorophyll A ◽  
Photosynthetic Pigments ◽  
Carotenoid Content ◽  
Chlorophyll B ◽  
Total Chlorophyll ◽  
Total Chlorophyll Content ◽  
Phosphorus Stress ◽  
Sagittaria Sagittifolia ◽  
Chlorophyll A Content

The photosynthetic pigments of Sagittaria sagittifolia, including total chlorophyll content, chlorophyll a content, chlorophyll b content and carotenoid content, were investigated after 0, 7, 14 and 21 d exposure to 3 mg L-1 P. After 7 d P-exposure, total chlorophyll content, chlorophyll a content and chlorophyll b content increased significantly compared with the control, and then decreased with time extended. Carotenoid content increased significantly after 14 d P-exposure compared with the control, and then decreased slightly after 21 d P-exposure.

Variation in Chlorophyll a and b in the Seagrass Halodule in Central Visayas, Philippines

2014 ◽  
Vol 8 (1) ◽  
Author(s):  
BILLY THEODORUS WAGEY
Keyword(s):  
Chlorophyll Content ◽  
Chlorophyll A ◽  
Photosynthetic Activity ◽  
Light Availability ◽  
Experimental Manipulation ◽  
Marine Ecology ◽  
Chlorophyll A And B ◽  
Photosynthetic Performances ◽  
Plastic Responses ◽  
One Way Anova

Chlorophyll content of seagrass leaves varies among species and habitats byat least a factor of five, and shows plastic responses to seasonal variations in light availability as well as direct experimental manipulation. This study generally attempted to observe the relative amount of chlorophyll a, b content of Haloduleuninervis and Halodule pinifolia in Central Visayas, Philippines in relation tothese environmental factors which are: exposure, density, temperature, and lightintensity. Chlorophyll was determined using the equation of Jeffrey and Humprey(1975) whereas data gathered went through one way ANOVA followed byTukey’s Test. Significant variations were found in most photosynthetic parametersmeasured in this study. Overall, H. uninervis and H. pinifolia seems to obtainoptimal photosynthetic performances over a broad range of irradiances. Mainly,genus Halodule can be seen as a highlight species, which has also the ability to acclimate to low irradiances. in general, the result indicated that seagrasses tend to decrease the ratio of chlorophyll a:b by increasing the amount of chlorophyllb to compensate for the decline in light intensity in subtidal areas. Therefore, H.uninervis and H. pinifolia seem to have the ability of adjusting their chlorophyllcontent in performing optimal photosynthetic activity over a broad range of irradiance regimes.Keywords: Marine Ecology, chlorophyll content, seagrass, Halodule, experimental design,Philippines

scholarly journals COMPARATIVE CHARACTERISTICS OF PHOTOSYNTHETIC ACTIVITY OF NECTARINE CULTIVARS AND FORMS WITH DIFFERENT COLORED LEAF PLATE

AGROFOR ◽  
2018 ◽  
Vol 2 (1) ◽  
Author(s):  
Iuliia IVASHCHENKO ◽  
Yuriy IVASHCHENKO ◽  
Evgeny SHOFERISTOV ◽  
Sergei TSIUPKA
Keyword(s):  
Powdery Mildew ◽  
Chlorophyll A ◽  
Energy Supply ◽  
Photosynthetic Activity ◽  
Green Color ◽  
Primary Reactions ◽  
Chlorophyll A And B ◽  
Traditional Cultivars ◽  
The Stability ◽  
Kautsky Effect

Genotypes of nectarine with red leaves were created in Nikita botanical gardens.They have commercially valuable properties and biological characteristics (resistanceto powdery mildew, large-fruited, the ability of transmitting the trait of redleaves with constant result to progeny seed, etc. ). The aim of our researches was tostudy the main indicators of photoactivity of leaf apparatus of nectarine with greenand anthocyanin leaves coloration. Investigations have been carried out in 2010-2015 on intact leaf plates for three cultivars and forms of nectarine with greenleaves – Rubinoviy-8 (control), Chemus, Krymtsuht 53-85 and 2 forms withanthocyanin coloration of leaf plate (NektadianaKrasnolistnaya 996-88, Krasnola495-86). Photosynthetic activity was characterized by chlorophyll fluorescenceparameters (Kautsky effect). The content of chlorophyll a and b were determinedby spectrophotometry. The cultivar Rubinoviy-8 and the form - Krasnola 495-86were selected according to intensity indicators of the primary reactions ofphotosynthesis. Anthocyanin forms concede cultivar Rubinoviy-8 in efficiency ofthe primary reactions of photosynthesis an average of 48-50%; but they exceednectarines Chemus and Krymtsuht 53-85 with green color of leaves of 23-25%.The efficiency of energy supply in the "dark" reactions of photosynthesis in allvarieties and forms of nectarine maintained within 41-46%. But the further abilityto utilize received energy is most efficiently implemented at the nectarine cultivarRubinoviy-8 and form Krasnola 495-86. The forms of red-leaved nectarine incomparison with traditional cultivars are distinguished by a great potential forretaining the stability and productivity of the functioning of the photosyntheticapparatus.

scholarly journals ANALISIS KANDUNGAN KLOROFIL PADA BERBAGAI POSISI DAUN DAN ANAK DAUN AREN (Arrenga pinnata)

2020 ◽  
Vol 12 (2) ◽  
Author(s):  
Olivia P. G. Lawendatu ◽  
Julius Pontoh ◽  
Vanda Kamu
Keyword(s):  
Chlorophyll Content ◽  
Chlorophyll A ◽  
Palm Tree ◽  
Plant Canopy ◽  
Chlorophyll B ◽  
Chlorophyll A And B ◽  
Palm Leaf

ABSTRAKTelah dilakukan penelitian tentang analisis kandungan klorofil dari berbagai posisi daun dan anak daun dari tanaman aren. Daun aren diambil pada posisi atas, tengah, dan bawah dari tajuk tanaman. Sampel anak daun  dibagi 3 posisi yaitu atas, tengah, dan bawah. Ekstraksi dilakukan dengan methanol dan penetuan klorofil pada daun dilakukan dengan spektofotometer pada panjang gelombang 665 nm dan 652 nm untuk masing-masing klorofil a dan klorofil b. Kandungan klorofil a dan b  yang terdapat pada sampel daun aren yang dihitung berdasarkan 3 unit satuan µg/mL, mg/g  dan µmol/m2. Kandungan klorofil a dan b pada sampel pohon aren tertinggi terletak pada posisi kiri daun, pada posisi daun terletak pada daun atas, pada posisi anak daun terletak pada posisi ujung anak daun. Ratio kandungan klorofil pada sampel daun aren berkisar 1,30-6,72. ABSTRACTHas conducted research on the analysis of chlorophyll content from various positions of leaves and leaves of palm plants. Leaves are taken in the top, middle and bottom positions of the plant canopy. Samples of leaflets were divided into 3 positions namely top, middle and bottom. Extraction was carried out with methanol and the determination of chlorophyll in the leaves was carried out by spectrophotometer at wavelengths of 665 nm and 652 nm for chlorophyll a and chlorophyll b, respectively. The chlorophyll and b content in palm leaf samples are calculated based on 3 units of μg/mL, mg/g, and µmol/m2. The content of chlorophyll a and b in the highest palm tree sample is located in the left position of the leaf, the position of the leaf is placed on the top of the leaf, the position of the leaflets is placed in the tip of the leaflets. The ratio of chlorophyll content in sugar palm leaf samples in the setting is 1.30-6.72. 

scholarly journals A study of the low-lying singlet and triplet electronic states of chlorophyll A and B

2013 ◽  
Vol 78 (11) ◽  
pp. 1775-1787 ◽  
Author(s):  
Mihajlo Etinski ◽  
Milena Petkovic ◽  
Miroslav Ristic
Keyword(s):  
Chlorophyll A ◽  
Density Functional ◽  
Chlorophyll B ◽  
Fluorescence Lifetimes ◽  
Functional Theory ◽  
Chlorophyll A And B ◽  
Vertical Ionization Potentials ◽  
Density Functional Theory Optimization ◽  
Triplet Formation ◽  
Good Agreement

Chlorophylls have been extensively investigated both experimentally and theoretically owing to the fact that they are essential for photosynthesis. We have studied two forms of chlorophyll, chlorophyll a and chlorophyll b, by means of density functional theory. Optimization of S0, S1 and T1 states was performed with the B3-LYP functional. The computed fluorescence lifetimes show good agreement with the available experimental data. The electronic adiabatic energies of S1 and T1 states are 2.09/2.12 and 1.19/1.29 eV for chlorophyll a and chlorophyll b respectively. We discussed the implications of this results on the triplet formation. Also, the calculated vertical ionization potentials shows good agreement with the experimental results.

scholarly journals Genetic analysis of chlorophyll content in maize by mixed major and polygene models

Genetika ◽  
2014 ◽  
Vol 46 (3) ◽  
pp. 1037-1046 ◽  
Author(s):  
Muhammad Irfan ◽  
Jia-Xing Sun ◽  
Yanbin Liu ◽  
Xue Li ◽  
Shuang Yang
Keyword(s):  
Genetic Analysis ◽  
Chlorophyll Content ◽  
Chlorophyll A ◽  
Segregation Analysis ◽  
Major Gene ◽  
Chlorophyll B ◽  
Major Genes ◽  
Main Gene ◽  
Joint Segregation ◽  
Joint Segregation Analysis

Chlorophyll is an important factor which also affects the yield in maize. In this study, genetic analysis of chlorophyll content was conducted by joint segregation analysis of four generations P1, P2, F1 and F2:4 from the cross Shen3336?Shen3265 using the mixed major genes and polygenes inheritance models. Genetics of chlorophyll revealed that chlorophyll ?a? was controlled by two main gene having additive-dominanceepistasis effects. The heritability of these genes were 56.3%. Chlorophyll ?b? was controlled by two pairs of codominant major gene plus additive-dominance polygene. The heritability of these major genes and polygenes were 1.12% and 93.26% respectively. Chlorophyll ?a+b? was controlled by two pairs of additive-dominance- epistatic major genes plus additive-dominance polygene having heritability of 56.2% and 5.2% respectively.

scholarly journals INFLUENCE OF PRE-SOWING TREATMENT OF SEEDS WITH COMPOSITIONS OF METABOLICALLY ACTIVE SUBSTANCES ON THE CONTENT OF PHOTOSYNTHETIC PIGMENTS IN THE LEAVES OF WINTER RYE PLANTS OF SYNTHETIC 38 AND ZABAVA VARIETIES AT DIFFERENT STAGES OF ONTOGENESIS

2021 ◽  
Vol 4 (11(75)) ◽  
pp. 11-16
Author(s):  
А. Kurylenko ◽  
O. Kurylenko ◽  
О. Kuchmenko ◽  
V. Havii
Keyword(s):  
Vitamin E ◽  
Chlorophyll A ◽  
Photosynthetic Pigments ◽  
Total Content ◽  
Winter Rye ◽  
Plant Leaves ◽  
Active Compounds ◽  
Chlorophyll A And B ◽  
Ubiquinone 10 ◽  
Active Substances

Purpose. The aim of the study is to investigate the effect of pre-sowing treatment of winter rye seeds of Synthetic 38 and Zabava with compositions of metabolically active substances on the content of photosynthetic pigments in plant leaves at different phases of ontogenesis. Materials and methods. The research materials were winter rye varieties Synthetic 38 and Zabava, and combinations of metabolically active substances: vitamin E (10-8M), paraoxybenzoic acid (0,001%), methionine (0,001%), ubiquinone-10 (10-8M) and MgSO4 (0,001%). The research scheme provided 4 options: 1) control (untreated seeds); 2) seeds, treated with a composition of substances: vitamin E + paraoxybenzoic acid + methionine + MgSO4; 3) seeds treated with a composition of substances: vitamin E + paraoxybenzoic acid + methionine; 4) seeds, processing by composition of substances: vitamin E + ubiquinone-10. Studies were conducted in the following phases of rye development: tillering, tubing, earing, flowering. The content of pigments - chlorophyll a, b and the total content of chlorophyll in the leaves of rye plants was determined by spectrophotometric method. Results and conclusions. It was demonstrated for the first time that the use of compositions of metabolically active compounds for pre-sowing treatment of winter rye varieties Synthetic 38 and Zabava leads to an increase of main chlorophyll, chlorophyll a and b in plant leaves in the dynamics from tillering to flowering. The composition consisting of vitamin E, para-oxybenzoic acid, methionine and MgSO4 showed the greatest efficiency. The effectiveness of this composition may be due to the complex action of all metabolically active compounds that are part of it. The obtained results may indicate a potentially more efficient absorption and conversion of energy by plants and the positive effect of these compositions on the photosynthesis of winter rye plants. Substances that have shown their effectiveness can be used as components of stimulants.

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