Comparative Evaluation of Two Automated Multiplex RT-PCR Tests for Rapid Detection of Influenza and Respiratory Syncytial Viruses

Abstract Background Infections caused by carbapenem-resistant Enterobacterales (CREs) are an emerging problem associated with high rates of morbidity and mortality. CREs are divided into two categories (carbapenemase-producing [CP] CRE and non-CP CRE). The most prevalent carbapenemase produced by Enterobacterales is Klebsiella pneumoniae carbapenemase (KPC) in Korea. Rapid identification of CREs is clinically important in infection control precaution. We compared the performance of two chromogenic media (chromID CARBA agar and CHROMagar KPC agar) for non-CP CREs or CP CREs with blaGES-5, blaNDM-1 or blaVIM-2 in a Korean hospital. Methods The study was carried out during a 3-month period from April to June 2017 during the surveillance program for CRE colonization. Antimicrobial susceptibility testing (AST) and polymerase chain reaction (PCR) were performed at the Korean Centers for Disease Control and Prevention. Results A total of 45 rectal swabs from 42 hospitalized patients were examined. Sensitivity of both chromID CARBA and CHROMagar KPC were 100% for CP CREs; and 50% and 100% for non-CP CREs, respectively. Specificity of chromID CARBA and CHROMagar KPC were 89.2% and 70.3% for CP CRE, respectively; and 76.9% and 66.7% for non-CP CRE, respectively. Conclusions The CHROMagar KPC is useful to monitor non-CP and CP CREs. The chromID CARBA is efficient for rapid detection of CP CREs requiring high contact precaution.

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Comparative evaluation of the microplate nitrate reductase assay and the rezasurin microtitre assay for the rapid detection of multidrug resistant Mycobacterium tuberculosis clinical isolates

Memórias do Instituto Oswaldo Cruz ◽

10.1590/s0074-02762012000500002 ◽

2012 ◽

Vol 107 (5) ◽

pp. 578-581 ◽

Cited By ~ 7

Author(s):

Ahmet Yilmaz Coban ◽

Meltem Uzun ◽

Alper Akgunes ◽

Belma Durupinar

Keyword(s):

Mycobacterium Tuberculosis ◽

Nitrate Reductase ◽

Rapid Detection ◽

Comparative Evaluation ◽

Multidrug Resistant ◽

Clinical Isolates ◽

Nitrate Reductase Assay

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A single-tube RT-PCR for rapid detection and differentiation of some African isolates of palyam serogroup orbiviruses

Journal of Virological Methods ◽

10.1016/j.jviromet.2009.05.019 ◽

2009 ◽

Vol 161 (1) ◽

pp. 70-74 ◽

Cited By ~ 6

Author(s):

Imadeldin E. Aradaib ◽

Mohamed E.H. Mohamed ◽

Mohamed A. Abdalla

Keyword(s):

Rapid Detection ◽

Rt Pcr ◽

Single Tube

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New Perspectives for SARS-CoV-2 Rapid Detection

Coronaviruses ◽

10.2174/2666796702666210308114737 ◽

2021 ◽

Vol 02 ◽

Author(s):

Latifa Khattabi ◽

Mustapha Mounir Bouhenna ◽

Feriel Sellam

Keyword(s):

Rapid Detection ◽

High Sensitivity ◽

The Other ◽

Host Cells ◽

Rt Pcr ◽

Human Immune System ◽

Virus Attachment ◽

Testing Procedures ◽

Diagnostic Kits ◽

Human Immune

: The present paper elucidates the conceivable application of two key molecules in SARS-CoV-2 detection of suspected infected persons. These molecules were selected from the basis of ACE-2 and S protein strong interaction that allows virus attachment to its host cells, on the other hand specific immunocompetant effectors generated by human immune system during the infection. Several testing procedures are already used to diagnose SARS-CoV-2 infection, particularly RT-PCR technique. ELISA and LFIA are possible assays for the employment of shACE-2/ hAc-anti-S (the molecules of interest) as the main agents of the test and confer a dual principal functions (capture and detection). The future diagnostic kits involving shACE-2 and hAc-anti-S will have the particularity of high sensitivity and rapid detection in addition to its advantage of relatively easy conception. It could be largely considered as a technical advanced kits in regards to the current SARS-CoV-2 diagnostic immunoassays.

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Comparative evaluation of conventional RT-PCR and real-time RT-PCR (RRT-PCR) for detection of avian metapneumovirus subtype A

Ciência Rural ◽

10.1590/s0103-84782009005000057 ◽

2009 ◽

Vol 39 (5) ◽

pp. 1445-1451 ◽

Cited By ~ 5

Author(s):

Helena Lage Ferreira ◽

Fernando Rosado Spilki ◽

Márcia Mercês Aparecida Bianchi dos Santos ◽

Renata Servan de Almeida ◽

Clarice Weis Arns

Keyword(s):

Real Time ◽

Comparative Evaluation ◽

Virus Isolation ◽

Diagnostic Methods ◽

N Gene ◽

Genomic Rna ◽

Rt Pcr ◽

Avian Metapneumovirus ◽

Lower Detection ◽

Subtype A

Avian metapneumovirus (AMPV) belongs to Metapneumovirus genus of Paramyxoviridae family. Virus isolation, serology, and detection of genomic RNA are used as diagnostic methods for AMPV. The aim of the present study was to compare the detection of six subgroup A AMPV isolates (AMPV/A) viral RNA by using different conventional and real time RT-PCR methods. Two new RT-PCR tests and two real time RT-PCR tests, both detecting fusion (F) gene and nucleocapsid (N) gene were compared with an established test for the attachment (G) gene. All the RT-PCR tested assays were able to detect the AMPV/A. The lower detection limits were observed using the N-, F- based RRT-PCR and F-based conventional RT-PCR (10(0.3) to 10¹ TCID50 mL-1). The present study suggests that the conventional F-based RT-PCR presented similar detection limit when compared to N- and F-based RRT-PCR and they can be successfully used for AMPV/A detection.

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