TOXICITY OF DISPERSANT, OIL, AND DISPERSED OIL TO TWO MARINE ORGANISMS

1997 ◽  
Vol 1997 (1) ◽  
pp. 1010-1011 ◽  
Author(s):  
Ismail Gulec ◽  
Douglas A. Holdway
Keyword(s):  
Crude Oil ◽  
Sodium Dodecyl Sulphate ◽  
Zinc Sulphate ◽  
Sodium Dodecyl ◽  
Marine Organisms ◽  
Test Species ◽  
Oil Dispersant ◽  
Dispersed Oil ◽  
Marine Sand

ABSTRACT Acute lethal bioassays using semistatic conditions were conducted to assess the toxicity of crude oil, dispersant, and dispersed oil using the amphipod Allorchestes compressa as a test species. Sublethal bioassays (suppression of burying behavior over 24 hours of exposure) were conducted for these toxicants using the marine sand snail Polinices conicus. Both lethal and sublethal bioassays were also carried out for two reference toxicants: sodium dodecyl sulphate (SDS) and zinc sulphate.

COOPERATIVE STUDIES ON THE TOXICITY OF DISPERSANTS AND DISPERSED OIL TO MARINE ORGANISMS: A 3-YEAR FLORIDA STUDY

2001 ◽  
Vol 2001 (2) ◽  
pp. 1237-1241 ◽  
Author(s):  
Dana L. Wetzel ◽  
Edward S. Van Fleet
Keyword(s):  
Crude Oil ◽  
Standard Test ◽  
Sciaenops Ocellatus ◽  
Crude Oils ◽  
Continuous Exposure ◽  
Test Species ◽  
Dispersed Oil ◽  
Test Organisms ◽  
Flow Through ◽  
Exposure Conditions

ABSTRACT The present study was conducted to assess the toxicity of the water-accommodated fraction (WAF) and the chemically enhanced WAF (CE-WAF) of selected crude oils for both weathered and fresh oil. Test organisms included two standard test species, Mysidopsis bahia and Menidia beryllina, and a commercially important Florida marine fish, Sciaenops ocellatus. Tests ascertaining LC50 values were conducted under continuous exposure and spiked (declining exposure using flow-through toxicity chambers) conditions using Venezuelan Crude Oil (VCO), Prudhoe Bay Crude Oil (PBCO), and COREXIT® 9500 dispersant on the above species. Data suggest that the dispersant is less toxic than the WAF and CE-WAF of the tested crude oils. The toxicity of the CE-WAF of fresh VCO is similar to that of other oils under continuous exposure conditions, but may be slightly more toxic to some species under spiked exposure conditions. The CE-WAF of fresh VCO appears to be less toxic than the corresponding WAF for M. bahia, M. beryllina, and S. ocellatus. Fresh VCO appears to be much more toxic to M. bahia and M. beryllina than weathered VCO in spiked exposure tests for both the WAF and CE-WAF. The WAF of PBCO is apparently less toxic to the test organisms than the corresponding WAF of fresh VCO. The LC50 values of M. bahia with CE-WAF fractions of both fresh VCO and PBCO are similar, while the same PBCO CE-WAF fraction is less toxic for M. beryllina than fresh VCO CE-WAF. The toxicity of oils and dispersants were lowest in the spiked exposure weathered oil tests, which may be most representative of an oil spill under natural environmental conditions.

Toxicity of Dispersants and Dispersed Oil To An Alaskan Marine Organism

1999 ◽  
Vol 1999 (1) ◽  
pp. 1035-1038 ◽  
Author(s):  
Sara L. Rhoton ◽  
Robert A. Perkins ◽  
Zachary D. Richter ◽  
Christina Behr-Andres ◽  
Jon E. Lindstrom ◽  
...  
Keyword(s):  
Crude Oil ◽  
Oil Spills ◽  
Marine Organism ◽  
Oil Dispersant ◽  
Crab Larvae ◽  
Dispersed Oil ◽  
Tanner Crab ◽  
Exposure Testing ◽  
The University ◽  
Chionoecetes Bairdi

ABSTRACT The University of Alaska Fairbanks (UAF) conducted toxicity assays on Alaskan tanner crab larvae (Chionoecetes bairdi) using the oil dispersant Corexit 9500, Alaska North Slope (ANS) crude oil, and dispersed ANS crude oil. These tests were conducted in Seward, Alaska using filtered saltwater at ambient temperature (6°C) and salinity (35%c). Similar toxicity assays were conducted at UAF on the reference species Mysidopsis bahia and Menidia beryllina under standard testing conditions (25°C and 20%c salinity). The methods used for these tests were developed by the Chemical Response to Oil Spills: Ecological Research Forum (CROSERF) and involve both continuous and spiked (declining concentration) exposure testing regimes. Toxicity data, expressed as EC50, were calculated using the defined response of “Affected,” as the typical response was decreased phototatic response; death as an endpoint was not often observed. The larvae were evaluated and placed into the following categories: Alive, Affected, Mortally Affected, and Dead. Results suggest that the tanner crab larvae are generally more resistant (EC50 = 355 mg/L) than M. beryllina (LC50 = 205 mg/L) and less resistant than M. bahia (LC50 = 622 mg/L) to dispersant solutions under spiked exposure.

scholarly journals Effects of Crude Oil, Dispersant, and Oil-Dispersant Mixtures on Human Fecal Microbiota in an In Vitro Culture System

mBio ◽  
2012 ◽  
Vol 3 (5) ◽  
Author(s):  
Jong Nam Kim ◽  
Bong-Soo Kim ◽  
Seong-Jae Kim ◽  
Carl E. Cerniglia
Keyword(s):  
Crude Oil ◽  
Microbial Communities ◽  
Human Health ◽  
Real Time Pcr ◽  
Deepwater Horizon ◽  
Fecal Microbiota ◽  
Test System ◽  
Oil Dispersant ◽  
Dispersed Oil

ABSTRACT The Deepwater Horizon oil spill of 2010 raised concerns that dispersant and dispersed oil, as well as crude oil itself, could contaminate shellfish and seafood habitats with hazardous residues that had potential implications for human health and the ecosystem. However, little is known about the effects of crude oil and dispersant on the human fecal microbiota. The aim of this research was to evaluate the potential effects of Deepwater Horizon crude oil, Corexit 9500 dispersant, and their combination on human fecal microbial communities, using an in vitro culture test system. Fecal specimens from healthy adult volunteers were made into suspensions, which were then treated with oil, dispersant, or oil-dispersant mixtures under anaerobic conditions in an in vitro culture test system. Perturbations of the microbial community, compared to untreated control cultures, were assessed using denaturing gradient gel electrophoresis (DGGE), real-time PCR, and pyrosequencing methods. DGGE and pyrosequencing analysis showed that oil-dispersant mixtures reduced the diversity of fecal microbiota from all individuals. Real-time PCR results indicated that the copy numbers of 16S rRNA genes in cultures treated with dispersed oil or oil alone were significantly lower than those in control incubations. The abundance of the Bacteroidetes decreased in crude oil-treated and dispersed-oil-treated cultures, while the Proteobacteria increased in cultures treated with dispersed oil. In conclusion, the human fecal microbiota was affected differently by oil and dispersed oil, and the influence of dispersed oil was significantly greater than that of either oil or dispersant alone compared to control cultures. IMPORTANCE There have been concerns whether human health is adversely affected by exposure to spilled crude oil, which contains regulated carcinogens, such as polycyclic aromatic hydrocarbons. In this study, we determined the effect of BP Deepwater Horizon crude oil and oil dispersant on the human intestinal microbiota, since there is the potential that low-level residues of petrochemicals could contaminate seafood. The results of this study will increase our understanding of the ecophysiological changes in the microbial communities of the human gastrointestinal tract with respect to crude oil exposure.

scholarly journals Recovery of Crude Oil by Chemical Flooding Method using SDS and Gum Arabic Mixtures

2019 ◽  
Vol 35 (2) ◽  
pp. 571-576
Author(s):  
Ahmed Sony ◽  
Hamdan Suhaimi ◽  
Laili Che Rose
Keyword(s):  
Interfacial Tension ◽  
Crude Oil ◽  
Oil Recovery ◽  
Sodium Dodecyl Sulphate ◽  
Sodium Dodecyl ◽  
Gum Arabic ◽  
Oil Reservoirs ◽  
Chemical Flooding ◽  
Residual Oil ◽  
End Products

A group of chemicals known as surfactants are widely used in industries. Their presence in any formulation, albeit little, exhibited superior functionality of the end-products. The dual hydrophobic and hydrophilic moiety of the structure have been shown to be responsible for reduction of surface/ interfacial tension and formation of micelles. In this work, a chemical flooding method using sodium dodecyl sulphate, SDS and its mixture with gum arabic, were carried out to study the recovery and efficiency of extracting the residual oil from the oil reservoirs. Two sets of experiments namely SDS and its mixture with gum arabic flooding at concentrations of SDS between 0.1-0.6 percent by weight are conducted. The percentage of gum arabic used is 16 percent by weight. Results shows that the use of SDS-Gum arabic flooding method yielded higher extraction of oil about 4.0 percent compared to SDS flooding. This suggests that the use of SDS and gum Arabic mixture is more efficient in increasing the amount of oil recovery.

scholarly journals Micellization behaviour of sodium dodecyl sulphate in presence and absence of sodium sulphate and zinc sulphate in distilled water by surface tension measurement

BIBECHANA ◽  
2014 ◽  
Vol 11 ◽  
pp. 79-85 ◽  
Author(s):  
Kuber Limbu ◽  
Sujit Kumar Shah ◽  
Ajaya Bhattarai
Keyword(s):  
Surface Tension ◽  
Critical Micelle Concentration ◽  
Sodium Dodecyl Sulphate ◽  
Room Temperature ◽  
Zinc Sulphate ◽  
Sodium Dodecyl ◽  
Sodium Sulphate ◽  
Distilled Water ◽  
Surface Tension Measurement ◽  
Weight Method

The accurate measurements of surface tension of sodium dodecyl sulphate (SDS) in distilled water and in presence of 0.01M Na2SO4 and 0.01M ZnSO4 at room temperature were reported by drop weight method using a stalagmometer. The critical micelle concentration (cmc) of sodium dodecyl sulphate (SDS) in distilled water was obtained higher than in presence of Na2SO4 and ZnSO4. The decrease of cmc of sodium dodecyl sulphate in the presence of salts has been discussed. DOI: http://dx.doi.org/10.3126/bibechana.v11i0.10383 BIBECHANA 11(1) (2014) 79-85

COMPARATIVE TOXICITY OF OIL, DISPERSANT, AND DISPERSED OIL TO TEXAS MARINE SPECIES

2001 ◽  
Vol 2001 (2) ◽  
pp. 1243-1248 ◽  
Author(s):  
Chris Fuller ◽  
James S. Bonner
Keyword(s):  
Crude Oil ◽  
Marine Bacteria ◽  
Environmental Protection Agency ◽  
Oil Spills ◽  
Marine Species ◽  
Continuous Exposure ◽  
Relative Toxicity ◽  
Test Species ◽  
Oil Dispersant ◽  
Chemical Response

ABSTRACT Dispersants are one class of chemical response agents currently approved for use on offshore oil spills. However, questions persist regarding potential environmental risks of nearshore dispersant applications. To address these questions, the relative toxicity of weathered crude oil, dispersant, and weathered crude oil plus dispersant were compared. This study included one luminescent marine bacteria (Vibrio fisheri), two marine vertebrate (Cyprinodon variegatus and Menidia beryllina), and one invertebrate test species (Mysidopsis bahia). Both the vertebrate and invertebrate species were tested under spiked (short episodic) exposure regimes and 96-hour continuous exposure regimes using protocols developed by the Chemical Response to Oil Spills: Ecological Effects Research Forum (CROSERF) and U.S. Environmental Protection Agency (EPA), respectively. Toxicity to the marine bacteria was evaluated after a 15-minute exposure using the Microbics Microtox® system. Results showed no significant variance between the relative toxicity of solutions prepared with weathered crude oil only and weathered crude oil plus dispersant when evaluated with the vertebrate and invertebrate test species. However, oil only solutions were shown to be significantly more toxic to Vibrio fisheri than oil plus dispersant solutions. Data also indicated that constant exposures were significantly more toxic than declining exposures, which is generally consistent with time weighted exposure response evaluations. Microtox® data was comparable to both vertebrate and invertebrate test results suggesting that the method is suitable for toxicity field screening.

Identifikasi Daging Tikus Pada Produk Baso Dengan Metode Sodium Dodecyl Sulphate Polyacrylamide Gel Electrophoresis (SDS-PAGE)

2018 ◽  
Vol 26 (2) ◽  
pp. 058
Author(s):  
Anna P. Roswiem ◽  
Triayu Septiani
Keyword(s):  
Sodium Dodecyl Sulphate ◽  
Gel Electrophoresis ◽  
Polyacrylamide Gel Electrophoresis ◽  
Sodium Dodecyl ◽  
Polyacrylamide Gel ◽  
Sds Page

<em>Bahan<strong> </strong>baku untuk membuat baso adalah daging hewan, pada umumnya dari daging sapi, ayam, ikan dan babi. Di beberapa daerah di Indonesia terjadi kasus baso tikus. Tujuan penelitian ini adalah menguji ada tidaknya kandungan daging tikus pada produk baso yang dijual di pasar Cempaka Putih-Kecamatan Kramat Jakarta Pusat dan di pedagang baso atau mie baso di sekitar kampus Universitas YARSI Jakarta. Daging adalah protein salah satu metode untuk mengidentifikasi protein adalah metode Sodium Dodecyl Sulphate Polyacrylamide Gel Electrophoresis (SDS-PAGE).<strong> </strong>Hasil penelitian menunjukkan bahwa dari 6 sampel baso terindikasi ada 2 sampel baso dengan nomor 1 dan 5 yang dibuat dari campuran daging sapi dan tikus; ada 1 sampel baso dengan nomor 6 yang terbuat dari daging tikus; dan 2 sampel baso dengan nomor 2 dan 3 yang terbuat dari campuran sapi  dan babi, dan hanya 1 sampel baso dengan nomor sampel 4 yang benar-benar terbuat dari daging sapi.</em>

Ultrasound Effect on Molecules of Sodium Dodecyl Sulphate as Systems of Nanoparticles

2018 ◽  
Vol 10 (6) ◽  
pp. 06013-1-06013-5 ◽  
Author(s):  
I. G. Vorobiova ◽  
◽  
Yu. A. Mirgorod ◽  
A. S. Chekadanov ◽  
◽  
...  
Keyword(s):  
Sodium Dodecyl Sulphate ◽  
Sodium Dodecyl ◽  
Ultrasound Effect
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