The Toxicity of Gold, Silver, and Zinc Oxide Nanoparticles on LDH Enzyme in Male Mice

The acute and sub chronic toxicity effects of 25.16 nm intraperitoneally- injected zinc oxide nanoparticles (ZnO NPs) were evaluated. Albino male mice were exposed to three different doses (25, 50 ,and 100 mg/kg ), depending on the value of calculated LD50, for 2 and 4 weeks . Considerable changes in organ indexes were shown with a good relevance to the illustrated histopathological effects which ranged from multiple hemorrhagic foci in liver, mild swelling and dilatation in kidney tubules, thickening of intestinal villi, moderate interstitial pneumonia, especially with the high dose , and sever necrosis of seminiferous tubules in testes of all studied groups. Significant changes in both hematological and biochemical parameters as well as thyroid hormones were observed with a considerable increase in the levels of antioxidant enzymes, in dose and exposure time dependent manner. The highest accumulated Zn mean values were recorded in the small intestine, kidney, liver, and spleen, respectively, followed by testes , heart , lung , and brain. These values followed the same order of the dose dependent manner, which explains the adverse effects that were recorded. This study proved the ability of using organ indexes as good tools side by side with the biochemical indicators to explain the histopathological changes. This study also revealed some histopathological effects that were not previously recorded as a toxicological effect of ZnO NPs in animal models.

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Protective Role of Nrf2 in Zinc Oxide Nanoparticles-Induced Lung Inflammation in Female Mice and Sexual Dimorphism in Susceptibility

10.21203/rs.3.rs-1215458/v1 ◽

2022 ◽

Author(s):

Radwa Sehsah ◽

Wenting Wu ◽

Sahoko Ichihara ◽

Naozumi Hashimoto ◽

Cai Zong ◽

...

Keyword(s):

Zinc Oxide ◽

Proinflammatory Cytokines ◽

Lung Inflammation ◽

Zinc Oxide Nanoparticles ◽

Synergistic Effects ◽

Oxide Nanoparticles ◽

Mrna Levels ◽

Male Mice ◽

Zno Nps ◽

Female Mice

Abstract Background Zinc oxide nanoparticles (ZnO-NPs) are used in various products such as rubber, paint, and cosmetics. Our group reported recently that Nrf2 protein provides protection against ZnO-NPs-induced pulmonary inflammation in male mice. The present study investigated the effect of Nrf2 deletion on the lung inflammatory response in female mice exposed to ZnO-NPs. Methods Twenty-four female Nrf2−/− mice and the same number of female Nrf2+/+ mice were each divided into three equal groups and each exposed to ZnO-NPs at either 0, 10 or 30 µg/mouse by pharyngeal aspiration. Bronchoalveolar lavage fluid (BALF) and lungs were collected 14 days later to quantify protein level, number of inflammatory cells, and for scoring inflammation histopathologically. The mRNA levels of Nrf2-depedent antioxidant enzymes and proinflammatory cytokine in lung tissue were measured. Results Exposure to ZnO-NPs increased all types of BALF cells and lung inflammation scores in both of female Nrf2−/− and Nrf2+/+ mice, and Nrf2 deletion enhanced ZnO-NPs-induced increase in the number of eosinophils in BALF. Nrf2 deletion enhanced ZnO-NPs-induced downregulation of GR and upregulation of HO-1 and TNFα. Nrf2 deletion decreased mRNA levels of CAT, GcLc and NQO1 and increased that for GcLm and MT-2. ZnO-NPs dose-dependently increased the level of oxidized glutathione (GSSG), and mRNA levels of proinflammatory cytokines/chemokines; KC, MIP-2, IL-6, IL-1β and MCP-1 only in wild-type mice, and Nrf2 deletion decreased total glutathione levels and upregulated the above proinflammatory cytokines/chemokines regardless of level of exposure to ZnO-NPs. Taken together with our previous results in male mice, our results showed a lower susceptibility of females to lung inflammation, relative to males, irrespective of Nrf2 deletion, and that enhancement of ZnO-NPs-induced upregulation of HO-1 and TNFα and downregulation of GR by deletion of Nrf2 is specific to female mice. Conclusion We conclude that Nrf2 provides protection in female mice against increase in BALF eosinophils, probably through down-regulation of proinflammatory cytokines/chemokines and upregulation of oxidative stress-related genes. The study also suggests lower susceptibility to lung inflammation in female mice relative to their male counterparts and the synergistic effects of sex and exposure to ZnO-NPs on mRNA expression of GR, HO-1 or TNFα.

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Histopathological Effect of Zinc Oxide Nanoparticles on Kkidney and Liver Tissues in Albino Male Mice

Ibn AL- Haitham Journal For Pure and Applied Science ◽

10.30526/31.1.1844 ◽

2018 ◽

Vol 31 (1) ◽

pp. 9 ◽

Cited By ~ 1

Author(s):

Ruqayah Ali Salman

Keyword(s):

Zinc Oxide ◽

Zinc Oxide Nanoparticles ◽

Histopathological Examination ◽

Control Group ◽

Oxide Nanoparticles ◽

Male Mice ◽

Zno Nps ◽

Liver And Kidney ◽

Dose Levels ◽

Liver Tissues

Objective: The present study investigated the effects of different dose levels of Zinc oxide Nanoparticles (ZnO NPs) on the liver and kidney tissues in albino male mice. Methadology: ZnO NPs was administrated as a daily oral dose of (150, 350 mg/kg body weight) gavage for 2 weeks. Eighteen male mices were used by dividing them into three groups. Result: Histopathological examination of kidney and hepatic tissues treated with ZnO NPs showed toxicity changes compared with control group. Conclusion:This study demonstrated the ability of ZnO NPs to affect on kidney and liver tissues. Recommendation: More study needed to know the effect of different doses of nanoparticles on human health.

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Optical Characteristics of Zinc Oxide Nanoparticles with Surface Modification Using Polyethylene Glycol

Journal of Inorganic Materials ◽

10.15541/jim20140080 ◽

2014 ◽

Vol 29 (10) ◽

pp. 1039

Author(s):

TAN Man-Lin ◽

WANG Yan-Tao ◽

ZHANG Wei-Li ◽

FU Dong-Ju ◽

LI Dong-Shuang ◽

...

Keyword(s):

Zinc Oxide ◽

Surface Modification ◽

Polyethylene Glycol ◽

Zinc Oxide Nanoparticles ◽

Optical Characteristics ◽

Oxide Nanoparticles

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A comparative study of the antibacterial activity of Quercus robur (Ethanolic extract) and Zinc oxide nanoparticles on Salmonella (In vitro)

International Journal of Research in Pharmaceutical Sciences ◽

10.26452/ijrps.v9ispl1.1397 ◽

2018 ◽

Vol 9 (SPL1) ◽

Author(s):

Hams H. H. Alfattli ◽

Ghufran Zuhair Jiber ◽

Ghaidaa Gatea Abbass

Keyword(s):

Zinc Oxide ◽

Antibacterial Activity ◽

Zinc Oxide Nanoparticles ◽

Quercus Robur ◽

Ethanolic Extract ◽

Inhibitory Effect ◽

Significant Inhibition ◽

Oxide Nanoparticles ◽

Inhibition Zones

This study which designed to evaluate the inhibitory effect of Ethanolic extract of (Quercusrobur) and Zinc oxide nanoparticles on the growth of one genus of enterobacteriacae (Salmonella). In vitro. For this purpose graduate concentrates for plant extract (50, 100, 200, 400 )mg/ml which prepared and compared with Zinc oxide nanoparticles of different concentration (2, 1, 0.5, 0.25) μg/ml,and examined. The result showed that the studied medicinal plant has antibacterial activity against this bacteria which used. The result showed that the plant has good activity in decrease the growth of this bacteria. The results of the study also showed that the nano-ZnO has very effective antibacterial action against the studied bacteria which was Salmonella,nanoparticles concentrations lead to increasing in the inhibition zones of tested bacterial growth. We also study the effect of three antibiotics Lomefloxacin (LOM), Ciprofloxacin (SIP) and Rifampin (RA) and the result showed,in a comparison within the tested bacteria,Salmonella had a significant inhibition increase in Lomefloxacin ; the ciprofloxacin showed effect on tested bacteria. However,Rifampin does not show any effect on tested bacteria.

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