scholarly journals Histopathological Effect of Zinc Oxide Nanoparticles on Kkidney and Liver Tissues in Albino Male Mice

2018 ◽  
Vol 31 (1) ◽  
pp. 9 ◽  
Author(s):  
Ruqayah Ali Salman
Keyword(s):  
Zinc Oxide ◽  
Zinc Oxide Nanoparticles ◽  
Histopathological Examination ◽  
Control Group ◽  
Oxide Nanoparticles ◽  
Male Mice ◽  
Zno Nps ◽  
Liver And Kidney ◽  
Dose Levels ◽  
Liver Tissues

    Objective: The present study investigated the effects of different dose levels of Zinc oxide Nanoparticles (ZnO NPs) on the liver and kidney tissues in albino male mice. Methadology: ZnO NPs was administrated as a daily oral dose of (150, 350 mg/kg body weight) gavage for 2 weeks. Eighteen male mices were used by dividing them into three groups. Result: Histopathological examination of kidney and hepatic tissues treated with ZnO NPs showed toxicity changes compared with control group. Conclusion:This study demonstrated the ability of ZnO NPs to affect on kidney and liver tissues. Recommendation: More study needed to know the effect of different doses of nanoparticles on human health.  

The Interaction of Zinc Oxide/Green Tea Extract Complex Nanoparticles and its Effect on Monosodium Glutamate Toxicity in Liver of Rats

2019 ◽  
Vol 20 (6) ◽  
pp. 465-475 ◽  
Author(s):  
Fawziah A. Al-Salmi ◽  
Reham Z. Hamza ◽  
Nahla S. El-Shenawy
Keyword(s):  
Oxidative Stress ◽  
Zinc Oxide ◽  
Green Tea ◽  
Zinc Oxide Nanoparticles ◽  
Monosodium Glutamate ◽  
Green Tea Extract ◽  
Control Group ◽  
Oxide Nanoparticles ◽  
Zno Nps ◽  
Tea Extract

Background: Zinc oxide nanoparticles (ZnO NPs) are increasingly utilized in both industrial and medical applications. Therefore, the study was aimed to investigate the effect of green nanoparticle complex (green tea extract/zinc oxide nanoparticles complex, GTE/ZnO NPs) on oxidative stress induced by monosodium glutamate (MSG) on the liver of rats. Methods: Wistar male rats (n=64) weighing between 200-250 g were divided randomly into eight groups: control group was given physiological saline (1 mg/kg), two groups were treated with two different doses of MSG (MSG-LD, MSG-HD; 6 and 17.5 mg/Kg, respectively), GTE was given 1 mg/mL, 5th group was treated with ZnO NPs and 6th group was treated with GTE/ZnO NPs complex while, 7th and 8th groups were treated with MSG-LD + GTE/ZnO NPs complex and MSG-HD + GTE/ZnO NPs complex, respectively. All substances were given orally for 30 consecutive days. At the end of the study, the liver was homogenized for measurement of the oxidative stress status and anti-inflammatory biomarkers as well as histological and transmission alternations. Results: Results showed that the antioxidant enzymes activity and glutathione level were significantly decreased in MSG groups than control in a dose-dependent manner. Conversely, the malondialdehyde and inflammatory cytokines levels were significantly increased in MSG groups than the control group. The liver indicated no evidence of alteration in oxidative status, anti-inflammatory and morphological parameters in GTE, ZnO NPs and GTE/ZnO NPs complex groups. Conclusion: In conclusion, MSG at both doses caused oxidative stress and inflammation on liver after 28 days of exposure that supported histological analysis and transmission view of hepatic parenchyma. GTE/ZnO NPs act as partial hepato-protective against MSG.

Comparative study of zinc oxide nanoparticles and its bulk form on liver function of Wistar rat

2019 ◽  
Vol 35 (10) ◽  
pp. 627-637 ◽  
Author(s):  
Eman Raafat Moatamed ◽  
Aida Ahmed Hussein ◽  
Mohamed Mahmoud El-desoky ◽  
Zakaria EL Khayat
Keyword(s):  
Zinc Oxide ◽  
Liver Function ◽  
Zinc Oxide Nanoparticles ◽  
Liver Enzymes ◽  
Histopathological Examination ◽  
Reduction Process ◽  
Wistar Rat ◽  
Oxide Nanoparticles ◽  
Zno Nps ◽  
Oxidation Reduction

Zinc oxide nanoparticles (ZnO NPs) are produced in high tonnage each year; they are widely used in consumer and industrial products, also now finding applications in bioimaging and drug delivery. In the present study, comparison between ZnO NPs (39 nm) and its bulk/micron form (particle size = 5 µm) on liver function of rats was determined. In our study, liver enzymes biomarkers, serum lipid profile, zinc concentration, and histopathological examination in liver tissues were used to evaluate liver injury. Moreover, lipid peroxidation (malondialdehyde), nitric oxide, and reduced glutathione levels were determined to detect the oxidation–reduction process in liver tissue. The results showed dose-dependent toxicity of ZnO NPs. Three different dose levels (25, 50, and 100 mg/kg bw) were used, and the 100-mg/kg bw ZnO NPs group showed the most significant changes in liver enzymes and histopathological structure, as well as redox state. The dose of 100 mg/kg bw of ZnO bulk group showed no significant effects on liver function. The study concluded that ZnO NPs caused hepatic impairments.

scholarly journals Zinc Oxide Nanoparticles Protected with Terpenoids as a Substance in Redox Imbalance Normalization in Burns

2021 ◽  
Vol 14 (6) ◽  
pp. 492
Author(s):  
Nina Melnikova ◽  
Alyona Balakireva ◽  
Dmitry Orekhov ◽  
Denis Kamorin ◽  
Natalia Didenko ◽  
...  
Keyword(s):  
Zinc Oxide ◽  
Zinc Oxide Nanoparticles ◽  
Hysteresis Loops ◽  
Fold Increase ◽  
Redox Balance ◽  
Healing Time ◽  
Control Group ◽  
Oxide Nanoparticles ◽  
Zno Nps ◽  
Ethanol Medium

Preliminary protection of zinc oxide nanoparticles (ZnO NPs) with terpenoids such as betulin, its derivatives, and essential oils components has been proposed to produce gel-like oleophilic and hydrophilic formulations. We studied the properties of gel-like dispersions of ZnO NPs with immobilized terpenoids and their effects on the activity of LDH, GR, G6PDH, restoration of redox balance of co-enzyme pairs NAD+/NADH and NADP+/NADPH, as well as the activity of SOD, catalase, AlDH in erythrocytes in the treatment of burns in rats. Hysteresis loops on the rheograms of studied dispersions characterize their thixotropic properties. ZnO NPs with betulin diphosphate in the water–ethanol medium lead to a 20-fold increase in the hydrodynamic radius at pH 7.3 compared to the initial ZnO NPs, and facilitate the formation of Zn2+ ions and their penetration into the viable epidermis, unlike oleophilic dispersions. All dispersions reduce the healing time by one and a half times compared with the untreated control group, increase the activity of LDH, GR, G6PDH, SOD, catalase, AlDH, and contribute to the normalization of coenzyme balance. Normalization of the redox balance and wound state was more effective using hydrophilic dispersions due to Zn2 + penetration.

scholarly journals Zinc Oxide Nanoparticles and Zinc Sulfate Impact Physiological Parameters and Boosts Lipid Peroxidation in Soil Grown Coriander Plants (Coriandrum sativum)

Molecules ◽  
2021 ◽  
Vol 26 (7) ◽  
pp. 1998
Author(s):  
Norma Ruiz-Torres ◽  
Antonio Flores-Naveda ◽  
Enrique Díaz Barriga-Castro ◽  
Neymar Camposeco-Montejo ◽  
Sonia Ramírez-Barrón ◽  
...  
Keyword(s):  
Zinc Oxide ◽  
Zinc Oxide Nanoparticles ◽  
Zinc Sulfate ◽  
Atomic Emission ◽  
Coriandrum Sativum ◽  
Emission Spectrometry ◽  
Control Group ◽  
Oxide Nanoparticles ◽  
Zno Nps ◽  
Chlorophyll Accumulation

The objective of this study was to determine the oxidative stress and the physiological and antioxidant responses of coriander plants (Coriandrum sativum) grown for 58 days in soil with zinc oxide nanoparticles (ZnO NPs) and zinc sulfate (ZnSO4) at concentrations of 0, 100, 200, 300, and 400 mg of Zn/kg of soil. The results revealed that all Zn compounds increased the total chlorophyll content (CHLt) by at least 45%, compared to the control group; however, with 400 mg/kg of ZnSO4, chlorophyll accumulation decreased by 34.6%. Zn determination by induction-plasma-coupled atomic emission spectrometry (ICP–AES) showed that Zn absorption in roots and shoots occurred in plants exposed to ZnSO4 at all concentrations, which resulted in high levels of hydrogen peroxide (H2O2) and malondialdehyde (MDA). Only at 400 mg/kg of ZnSO4, a 78.6% decrease in the MDA levels was observed. According to the results, the ZnSO4 treatments were more effective than the ZnO NPs to increase the antioxidant activity of catalase (CAT), ascorbate peroxidase (APX), and peroxidases (POD). The results corroborate that phytotoxicity was higher in plants subjected to ZnSO4 compared to treatments with ZnO NPs, which suggests that the toxicity was due to Zn accumulation in the tissues by absorbing dissolved Zn++ ions.

scholarly journals The Effects of Pentoxifylline on Memory in Male Rats Treated with Zinc Oxide NPs

2021 ◽  
Vol 25 (1) ◽  
pp. 1-10
Author(s):  
Niloufar Darbandi ◽  
◽  
Zeynab Vasheghani Farahani ◽  
Hamidreza Momeni ◽  
◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Zinc Oxide ◽  
Treatment Group ◽  
Blood Serum ◽  
Zinc Oxide Nanoparticles ◽  
Male Rats ◽  
Control Group ◽  
Oxide Nanoparticles ◽  
Zno Nps ◽  
Ca1 Region

Background: Zinc oxide Nanoparticles (NPs) present irreversible effects on the nervous system, memory, and learning. Objective: The current study aimed to investigate the effects of pentoxifylline on memory impairments, CA1 hippocampal pyramidal cells, and blood serum antioxidant enzymes in male rats treated with zinc oxide NPs. Methods: Male Wistar rats were divided into the control, zinc oxide NPs (1.25 mg/kg), pentoxifylline (50 mg/kg), and pentoxifylline with zinc oxide NPs groups. In all study groups, saline, zinc oxide NPs, and pentoxifylline were intraperitoneally injected 30 minutes before training. In the co-treatment group, pentoxifylline was injected one hour before injecting Zno NPs. After performing the behavioral test, the tested animals’ brains were fixed and the number of healthy neurons in the CA1 region of the hippocampus was counted. In all research groups, malondialdehyde levels, total antioxidant power, superoxide dismutase levels, and glutathione peroxidase in blood serum were measured. Results: Zinc oxide nanoparticles decreased memory and the number of healthy neurons in the CA1 region of the hippocampus and increased oxidative stress in blood serum, compared to the controls. In the co-treatment group, using pentoxifylline improved the above-mentioned factors and reached the level of the control group. Pentoxifylline alone presented no significant effect on the aforementioned characteristics, compared to the control group. Conclusion: ZnO NPs may decrease memory retrieval and cause cell death in the pyramidal neurons of the CA1 region of the hippocampus by increasing oxidative stress. Pentoxifylline, as a potent antioxidant, can prevent the harmful effects of ZnO NPs.

scholarly journals Evaluation of the Toxicological Effects of Zinc Oxide Nanoparticles in Albino Male Mice

2020 ◽  
pp. 42-58
Author(s):  
Sarab Mohammed M. Razooki ◽  
Adel M. Rabee
Keyword(s):  
Zinc Oxide ◽  
Zinc Oxide Nanoparticles ◽  
Seminiferous Tubules ◽  
Oxide Nanoparticles ◽  
High Dose ◽  
Dependent Manner ◽  
Male Mice ◽  
Zno Nps ◽  
Toxicological Effect ◽  
Histopathological Effects

The acute and sub chronic toxicity effects of 25.16 nm intraperitoneally- injected zinc oxide nanoparticles (ZnO NPs) were evaluated. Albino male mice were exposed to three different doses (25, 50 ,and 100 mg/kg ), depending on the value of calculated LD50, for 2 and 4 weeks . Considerable changes in organ indexes were shown with a good relevance to the illustrated histopathological effects which ranged from multiple hemorrhagic foci in liver, mild swelling and dilatation in kidney tubules, thickening of intestinal villi, moderate interstitial pneumonia, especially with the high dose , and sever necrosis of seminiferous tubules in testes of all studied groups. Significant changes in both hematological and biochemical parameters as well as thyroid hormones were observed with a considerable increase in the levels of antioxidant enzymes, in dose and exposure time dependent manner. The highest accumulated Zn mean values were recorded in the small intestine, kidney, liver, and spleen, respectively, followed by testes , heart , lung , and brain. These values followed the same order of the dose dependent manner, which explains the adverse effects that were recorded. This study proved the ability of using organ indexes as good tools side by side with the biochemical indicators to explain the histopathological changes. This study also revealed some histopathological effects that were not previously recorded as a toxicological effect of ZnO NPs in animal models.   

A Histological and Immunohistochemical Study on the Effect of Zinc Oxide Nanoparticles on Rat Lung Tissue

QJM ◽  
2021 ◽  
Vol 114 (Supplement_1) ◽  
Author(s):  
Nagwa Elshakaa ◽  
Nevine Bahaa ◽  
Asmaa Abo Zeid ◽  
Heba Abdel Latif
Keyword(s):  
Zinc Oxide ◽  
Lung Tissue ◽  
Zinc Oxide Nanoparticles ◽  
Control Group ◽  
Histological Structure ◽  
Oxide Nanoparticles ◽  
Rat Lung ◽  
Zno Nps ◽  
Group I ◽  
Group Ii

Abstract Background Nanoparticles (NPs) have unique and novel properties that lead to a diverse array of products with applications in diagnosis, drug delivery, food industry, paints, electronics, environmental cleanup, cosmetics and sunscreens. Zinc oxide nanoparticles (ZnO NPs) are one of the most widely used engineered nanoparticles in consumer products. They are utilized in many commercial products including cosmetics, paints, textiles, and personal hygiene products. The study aimed to assess the effects of zinc oxide nanoparticles administered Intranasal or intravenous on lung tissue. Materials & methods twenty-five male Wistar rats were divided into Group I; control group, group II (Intranasal administered group) group II (Intravenous administered group). The animals were injected with 4 mg/kg of ZnO NPs. Rat Lungs were processed for histological, immunohistochemical. Results ZnO NPs caused thickening of interalveolar septa. Mononuclear cells were seen infiltrating the interalveolar septa. Many dilated blood vessels exhibited focal disruption and focal thickening of their wall. Tumor necrosis factor-alpha (TNF-α) immune reactivity was significantly increased. These findings increased mainly in the intranasal administered group when compared with the intravenous group. Conclusion ZnO NPs administration caused toxic effects on the histological structure of albino rat lung.

A comprehensive toxicity study of zinc oxide nanoparticles versus their bulk in Wistar rats

2016 ◽  
Vol 35 (12) ◽  
pp. 1286-1304 ◽  
Author(s):  
Anurag Kumar Srivastav ◽  
Mahadeo Kumar ◽  
Nasreen Ghazi Ansari ◽  
Abhishek Kumar Jain ◽  
Jai Shankar ◽  
...  
Keyword(s):  
Zinc Oxide ◽  
Zinc Oxide Nanoparticles ◽  
Wistar Rats ◽  
Hematological Parameters ◽  
Control Group ◽  
Oxide Nanoparticles ◽  
Oral Toxicity ◽  
Zno Nps ◽  
Toxicological Effect ◽  
The Impact

The purpose of this study was to characterize the zinc oxide nanoparticles (ZnO-NPs) and their bulk counterpart in suspensions and to access the impact of their acute oral toxicity at doses of 300 and 2000 mg/kg in healthy female Wistar rats. The hematological, biochemical, and urine parameters were accessed at 24 and 48 h and 14 days posttreatment. The histopathological evaluations of tissues were also performed. The distribution of zinc content in liver, kidney, spleen, plasma, and excretory materials (feces and urine) at 24 and 48 h and 14 days posttreatment were accessed after a single exposure at dose of 2000 mg/kg body weight. The elevated level of alanine amino transferase, alkaline phosphatase, lactate dehydrogenase, and creatinine were observed in ZnO-NPs at a dose of 2000 mg/kg at all time points. There was a decrease in iron levels in all the treated groups at 24 h posttreatment as compared to control groups but returned to their normal level at 14 days posttreatment. The hematological parameters red blood cells, hemoglobin, hematocrit, platelets, and haptoglobin were reduced at 48 h posttreatment at a dose of 2000 mg/kg ZnO-NPs and showed hemolytic condition. All the treated groups were comparable to control group at the end of 14 days posttreatment. The zinc concentration in the kidney, liver, plasma, feces, and urine showed a significant increase in both groups as compared to control. This study explained that ZnO-NPs produced more toxicological effect as compared to their bulk particles as evidenced through alteration in some hemato-biochemical parameters and with few histopathological lesions in liver and kidney tissues.

scholarly journals Protective effects of zinc oxide nanoparticles against doxorubicin induced testicular toxicity and DNA damage in male rats

2019 ◽  
Vol 8 (5) ◽  
pp. 654-662 ◽  
Author(s):  
Zeynab Khamis El-Maddawy ◽  
Walaa Slouma Hamouda Abd El Naby
Keyword(s):  
Zinc Oxide ◽  
Zinc Oxide Nanoparticles ◽  
Histopathological Examination ◽  
Sperm Count ◽  
Treated Group ◽  
Male Rats ◽  
Oxide Nanoparticles ◽  
Testicular Toxicity ◽  
Protective Effects ◽  
Zno Nps

Abstract The present study aims to investigate the protective effects of zinc oxide nanoparticles (ZnO NPs) on doxorubicin-induced testicular injury. Forty mature male rats were randomly allocated into four equal groups: G1 (control), G2 (3 mg per kg BW of zinc oxide nanoparticles was administered), G3 (6 mg per kg BW of doxorubicin was intraperitoneally injected), and G4 (doxorubicin + ZnO NPs). Some fertility parameters, antioxidant status, genotoxicity assay, and a histopathological examination were used for this investigation. The doxorubicin-treated group showed a significant decrease in the index weight of reproductive organs, epididymal sperm count, motility%, and live sperm% and a significant increase in sperm abnormalities. Moreover, GSH and CAT activities were significantly decreased, and MDA content was significantly increased in the doxorubicin-treated group. Interestingly, co-administration of ZnO NPs significantly reduced the doxorubicin-induced changes in the investigated parameters. In addition, ZnO NPs alone did not show any undesirable effects on the sperm parameters, testis or DNA. However, its administration improves the reproductive parameters and significantly increases the testosterone level. We concluded that the administration of ZnO NPs at 3 mg per kg BW ameliorated the testicular toxicity and genotoxicity caused by doxorubicin through its antioxidant and androgenic activity.

The Anti-Breast Cancer Effects of Green-Synthesized Zinc Oxide Nanoparticles Using Carob Extract

2020 ◽  
Vol 20 ◽  
Author(s):  
Vahid Pouresmaeil ◽  
Shaghayegh Haghighi ◽  
Asieh S. Raeisalsadati ◽  
Ali Neamati ◽  
Masoud Homayouni-Tabrizi
Keyword(s):  
Breast Cancer ◽  
Zinc Oxide ◽  
Zinc Oxide Nanoparticles ◽  
Quantitative Polymerase Chain Reaction ◽  
Breast Cancer Cell Lines ◽  
Control Group ◽  
Oxide Nanoparticles ◽  
Dependent Manner ◽  
Zno Nps ◽  
Dose Dependent

Background: The use of nanoparticles synthesized by the green method to treat cancer is fairly recent. The aim of this study was to evaluate cytotoxicity, apoptotic and anti-angiogenic effects and their expression of involving genes, of zinc oxide nanoparticles (ZnO-NPs) synthesized with Carob extract on different human breast cancer cell lines. Methods: ZnO-NPs synthesized using the extract of Carob and characterized with various analytical techniques. The MCF-7 and MDA-MB231 cells were treated at different times and concentrations with ZnO-NPs. The cytotoxicity, apoptosis and anti-angiogenic were examined using a series of cellular assays. Expression of apoptotic genes (Bax and Bcl2) and anti-angiogenic genes, vascular endothelial growth factor (VEGF) and its receptor (VEGF-R) in cancer cells treated with ZnO-NPs were examined with Reverse transcription-quantitative polymerase chain reaction (RT-qPCR). The antioxidant activities of ZnO-NPs evaluated by ABTS and DPPH assay. Results: Exposure of cells to ZnO-NPs resulted in a dose-dependent loss of cell viability. The IC50 at 24, 48 and 72 hours were 125, 62.5 and 31.2µg/ml respectively (p<0.001). ZnO-NPs treated cells showed in fluorescent microscopy that ZnONPs are able to upregulate apoptosis and RT-qPCR revealed upregulation of Bax (p<0.001) and downregulation of Bcl-2 (p<0.05). ZnO-NPs increased VEGF gene expression while decreasing VEGF-R (p<0.001). The antioxidant effects of ZnO-NPs were higher than control group and were dose dependent manner (p<0.001). Conclusion: ZnO-NPs synthetized using Carob extract have the ability to eliminate breast cancerous cells and inhibit angiogenesis so could be used as anticancer agent.

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