scholarly journals Evaluation of the Toxicological Effects of Zinc Oxide Nanoparticles in Albino Male Mice

2020 ◽  
pp. 42-58
Author(s):  
Sarab Mohammed M. Razooki ◽  
Adel M. Rabee
Keyword(s):  
Zinc Oxide ◽  
Zinc Oxide Nanoparticles ◽  
Seminiferous Tubules ◽  
Oxide Nanoparticles ◽  
High Dose ◽  
Dependent Manner ◽  
Male Mice ◽  
Zno Nps ◽  
Toxicological Effect ◽  
Histopathological Effects

The acute and sub chronic toxicity effects of 25.16 nm intraperitoneally- injected zinc oxide nanoparticles (ZnO NPs) were evaluated. Albino male mice were exposed to three different doses (25, 50 ,and 100 mg/kg ), depending on the value of calculated LD50, for 2 and 4 weeks . Considerable changes in organ indexes were shown with a good relevance to the illustrated histopathological effects which ranged from multiple hemorrhagic foci in liver, mild swelling and dilatation in kidney tubules, thickening of intestinal villi, moderate interstitial pneumonia, especially with the high dose , and sever necrosis of seminiferous tubules in testes of all studied groups. Significant changes in both hematological and biochemical parameters as well as thyroid hormones were observed with a considerable increase in the levels of antioxidant enzymes, in dose and exposure time dependent manner. The highest accumulated Zn mean values were recorded in the small intestine, kidney, liver, and spleen, respectively, followed by testes , heart , lung , and brain. These values followed the same order of the dose dependent manner, which explains the adverse effects that were recorded. This study proved the ability of using organ indexes as good tools side by side with the biochemical indicators to explain the histopathological changes. This study also revealed some histopathological effects that were not previously recorded as a toxicological effect of ZnO NPs in animal models.   

Evaluation of the Effect of Nanoparticles Zinc Oxide/Camellia sinensis Complex on the Kidney of Rats Treated with Monosodium Glutamate: Antioxidant and Histological Approaches

2019 ◽  
Vol 20 (7) ◽  
pp. 542-550 ◽  
Author(s):  
Nahla S. El-Shenawy ◽  
Reham Z. Hamza ◽  
Fawziah A. Al-Salmi ◽  
Rasha A. Al-Eisa
Keyword(s):  
Zinc Oxide ◽  
Camellia Sinensis ◽  
Zinc Oxide Nanoparticles ◽  
Morphological Changes ◽  
Monosodium Glutamate ◽  
Oxide Nanoparticles ◽  
High Dose ◽  
Enzymatic Antioxidants ◽  
Zno Nps ◽  
Tea Extract

Background: Zinc oxide nanoparticles (ZnO NPs) are robustly used biomedicine. Moreover, no study has been conducted to explore the consequence of green synthesis of ZnO NPs with Camellia sinensis (green tea extract, GTE) on kidneys of rats treated with monosodium glutamate (MSG). Methods: Therefore, the objective of the research was designed to explore the possible defensive effect of GTE/ZnO NPs against MSG-induced renal stress investigated at redox and histopathological points. Results: The levels of urea and creatinine increased as the effect of a high dose of MSG, in addition, the myeloperoxidase and xanthine oxidase activates were elevated significantly with the high dose of MSG. The levels of non-enzymatic antioxidants (uric acid, glutathione, and thiol) were decreased sharply in MSG-treated rats as compared to the normal group. Conclusion: The data displayed that GTE/ZnO NPs reduced the effects of MSG significantly by reduction of the level peroxidation and enhancement intracellular antioxidant. These biochemical findings were supported by histopathology evaluation, which showed minor morphological changes in the kidneys of rats.

scholarly journals Influence of NOM on the Stability of Zinc Oxide Nanoparticles in Ecotoxicity Tests

2020 ◽  
Vol 10 (18) ◽  
pp. 6431
Author(s):  
Byoung-cheun Lee ◽  
Gilsang Hong ◽  
Hyejin Lee ◽  
Pyeongsoon Kim ◽  
Do-Yeon Seo ◽  
...  
Keyword(s):  
Zinc Oxide ◽  
Zinc Oxide Nanoparticles ◽  
Aquatic Organisms ◽  
Test Method ◽  
Oxide Nanoparticles ◽  
Zno Nps ◽  
Ionization Degree ◽  
Toxicological Effect ◽  
Ecotoxicity Tests ◽  
Repulsive Forces

Nanomaterials are known to aggregate in the presence of ions. Similarly, the aggregation of zinc oxide nanoparticles (ZnO NPs) exposed to various ions such as sodium chloride and calcium chloride in water systems increases with the ionic strength. Therefore, for accurate toxicity studies, it is necessary to conduct a test using natural organic matters (NOMs) as additional dispersants that strengthen stability with increased repulsive forces. The three types of ecotoxicity tests based on the dispersion stability test using NOM showed that the toxicities of the three test samples decreased in the presence of NOM. To determine how NOM improved dispersion and reduced toxicities, we analyzed the ionization degree of ZnO NPs with and without NOM and found that the solubility was below 2 mg/L with a negligible change over time, implying that the ionization effect was low. The absolute value of the surface charge of particles increased in the presence of NOM, resulting in increased repulsive electrostatic forces and steric hindrance, causing less aggregation and more dispersion. Additionally, although the NOM used in the test is considered an effective dispersant that does not have a toxicological effect on aquatic organisms, the presence of NOM resulted in reduced toxicities and should be further investigated to establish it as a standard test method.

Genotoxicity evaluation of zinc oxide nanoparticles in Swiss mice after oral administration using chromosomal aberration, micronuclei, semen analysis, and RAPD profile

2017 ◽  
Vol 33 (11) ◽  
pp. 821-834 ◽  
Author(s):  
Anurag Kumar Srivastav ◽  
Akhilesh Kumar ◽  
Jyoti Prakash ◽  
Dhirendra Singh ◽  
Pankaj Jagdale ◽  
...  
Keyword(s):  
Zinc Oxide ◽  
Oral Administration ◽  
Chromosomal Aberration ◽  
Zinc Oxide Nanoparticles ◽  
Dose Group ◽  
High Dose Group ◽  
Oxide Nanoparticles ◽  
High Dose ◽  
Zno Nps ◽  
Swiss Mice

The expanded uses of zinc oxide nanoparticles (ZnO NPs) have grown rapidly in the field of nanotechnology. Thus, rising production of nanoparticles (NPs) increases the possible risks to the environment and occupationally exposed humans. Hence, it is indispensable to appraise the safety toxicity including genotoxicity for these NPs. In the present study, we have evaluated the genotoxic effect of ZnO NPs after oral administration to Swiss mice at dose levels of 300 and 2000 mg/kg body weight. These doses were administered for 2 days at 24 h apart. Chromosomal aberration (CA) and micronucleus tests were conducted following Organization for Economic Co-operation and Development guidelines. DNA damage was evaluated at 0, 24, 48, and 72 h posttreatment using a randomly amplified polymorphic DNA (RAPD) assay; additionally, semen analyses were also performed at 34.5 days post oral exposure. The reactive oxygen species (ROS), 8-oxo-2′-deoxyguanosine and CAs were increased ( p < 0.05) at the highest dosage (2000 mg/kg) of ZnO NPs compared to controls. Aberrant sperm morphology with reduced sperm count and motility were also present ( p < 0.05) in the high-dose group. Based on the RAPD assay, the genomic template stability within the high-dose group (<90%) was less than the controls (100%). The results suggested that ZnO NPs are mildly genotoxic in a dose-related manner and this toxicity were induced by generation of ROS.

scholarly journals Active Packaging of Immobilized Zinc Oxide Nanoparticles Controls Campylobacter jejuni in Raw Chicken Meat

2020 ◽  
Vol 86 (22) ◽  
Author(s):  
Mohammed J. Hakeem ◽  
Jinsong Feng ◽  
Azadeh Nilghaz ◽  
Luyao Ma ◽  
Hwai Chuin Seah ◽  
...  
Keyword(s):  
Zinc Oxide ◽  
Shelf Life ◽  
Campylobacter Jejuni ◽  
Zinc Oxide Nanoparticles ◽  
Chicken Meat ◽  
Oxide Nanoparticles ◽  
Dependent Manner ◽  
Zno Nps ◽  
Content Type ◽  
Active Food Packaging

ABSTRACT Zinc oxide nanoparticles (ZnO NPs) are regarded as a safe and stable antimicrobial that can inactivate bacteria by several potential working mechanisms. We aimed to incorporate ZnO NPs into packaging material to control Campylobacter in raw chicken meat. ZnO NPs were first incorporated into three-dimensional (3D) paper tubes to identify the lethal concentration against Campylobacter jejuni, which was selected as the working concentration to develop 2D functionalized absorbing pads by an ultrasound-assisted dipping technique. The functionalized pad was placed underneath raw chicken meat to inactivate C. jejuni and the predominant chicken microbiota at 4°C within 8 days of storage. Immobilized ZnO NPs at 0.856 mg/cm2 reduced C. jejuni from ∼4 log CFU/25 g raw chicken meat to an undetectable level after 3 days of storage. Analysis by inductively coupled plasma-optical emission spectroscopy showed that the Zn level increased from 0.02 to 0.17 mg/cm2 in treated raw chicken meat. Scanning electron microscopy validated the absence of nanoparticle migration onto raw chicken meat after treatment. Inactivation of C. jejuni was associated with the increase of lactic acid produced by Lactobacillus in raw chicken meat in a pH-dependent manner. Less than 5% of Zn2+ was released from ZnO NPs at neutral pH, while up to 88% was released when the pH was <3.5 within 2 days. Whole-transcriptome sequencing (RNA-Seq) analysis demonstrated a broad effect of ZnO NPs on genes involved in various cellular developmental processes as annotated by gene ontology. Taken together, the results indicate that functionalized absorbing pads inactivated C. jejuni in raw chicken meat by immobilized ZnO NPs along with the controllable released Zn2+. IMPORTANCE Prevalence of Campylobacter in raw poultry remains a major food microbiological safety challenge. Novel mitigation strategies are required to ensure the safety and quality of poultry products. Active food packaging can control pathogens without directly adding antimicrobials into the food matrix and extend the food’s shelf life. The functionalized absorbing pad with ZnO NPs developed in this study was able to inactivate C. jejuni in raw chicken meat and keep the meat free from C. jejuni contamination during shelf life without any observed migration of nanoparticles. The controllable conversion of immobilized ZnO NPs to free Zn2+ makes this approach safe and eco-friendly and paves the way for developing a novel intervention strategy for other high-risk foods. Our study applied nanotechnology to exploit an effective approach for Campylobacter control in raw chicken meat products.

scholarly journals Genotoxic and Cytotoxic Properties of Zinc Oxide Nanoparticles Phyto-Fabricated from the Obscure Morning Glory Plant Ipomoea obscura (L.) Ker Gawl

Molecules ◽  
2021 ◽  
Vol 26 (4) ◽  
pp. 891
Author(s):  
Mahadevamurthy Murali ◽  
Satish Anandan ◽  
Mohammad Azam Ansari ◽  
Mohammad A. Alzohairy ◽  
Mohammad N. Alomary ◽  
...  
Keyword(s):  
Zinc Oxide ◽  
Cell Division ◽  
Zinc Oxide Nanoparticles ◽  
Leaf Extract ◽  
Bandgap Energy ◽  
Oxide Nanoparticles ◽  
Dependent Manner ◽  
Zno Nps ◽  
Aqueous Leaf Extract ◽  
Ipomoea Obscura

The study was undertaken to investigate the antioxidant, genotoxic, and cytotoxic potentialities of phyto-fabricated zinc oxide nanoparticles (ZnO-NPs) from Ipomoea obscura (L.) Ker Gawl. aqueous leaf extract. The UV-visible spectral analysis of the ZnO-NPs showed an absorption peak at 304 nm with a bandgap energy of 3.54 eV, which are characteristics of zinc nanoparticles. Moreover, the particles were of nano-size (~24.26 nm) with 88.11% purity and were agglomerated as observed through Scanning Electron Microscopy (SEM). The phyto-fabricated ZnO-NPs offered radical scavenging activity (RSA) in a dose-dependent manner with an IC50 of 0.45 mg mL−1. In addition, the genotoxicity studies of ZnO-NPs carried out on onion root tips revealed that the particles were able to significantly inhibit the cell division at the mitotic stage with a mitotic index of 39.49%. Further, the cytotoxic studies on HT-29 cells showed that the phyto-fabricated ZnO-NPs could arrest the cell division as early as in the G0/G1 phase (with 92.14%) with 73.14% cells showing early apoptotic symptoms after 24 h of incubation. The results of the study affirm the ability of phyto-fabricated ZnO-NPs from aqueous leaf extract of I. obscura is beneficial in the cytotoxic application.

scholarly journals In Vitro Cytotoxicity Effects of Zinc Oxide Nanoparticles on Spermatogonia Cells

Cells ◽  
2020 ◽  
Vol 9 (5) ◽  
pp. 1081
Author(s):  
Ana Rita Pinho ◽  
Filipa Martins ◽  
M. Elisabete V. Costa ◽  
Ana M. R. Senos ◽  
Odete A. B. da Cruz e Silva ◽  
...  
Keyword(s):  
Zinc Oxide ◽  
Zinc Oxide Nanoparticles ◽  
The Body ◽  
Oxide Nanoparticles ◽  
Dependent Manner ◽  
Zno Nps ◽  
Cytotoxic Effects ◽  
Spermatogonia Cells ◽  
The Impact

Zinc Oxide Nanoparticles (ZnO NPs) are a type of metal oxide nanoparticle with an extensive use in biomedicine. Several studies have focused on the biosafety of ZnO NPs, since their size and surface area favor entrance and accumulation in the body, which can induce toxic effects. In previous studies, ZnO NPs have been identified as a dose- and time-dependent cytotoxic inducer in testis and male germ cells. However, the consequences for the first cell stage of spermatogenesis, spermatogonia, have never been evaluated. Therefore, the aim of the present work is to evaluate in vitro the cytotoxic effects of ZnO NPs in spermatogonia cells, focusing on changes in cytoskeleton and nucleoskeleton. For that purpose, GC-1 cell line derived from mouse testes was selected as a model of spermatogenesis. These cells were treated with different doses of ZnO NPs for 6 h and 12 h. The impact of GC-1 cells exposure to ZnO NPs on cell viability, cell damage, and cytoskeleton and nucleoskeleton dynamics was assessed. Our results clearly indicate that higher concentrations of ZnO NPs have a cytotoxic effect in GC-1 cells, leading to an increase of intracellular Reactive Oxygen Species (ROS) levels, DNA damage, cytoskeleton and nucleoskeleton dynamics alterations, and consequently cell death. In conclusion, it is here reported for the first time that ZnO NPs induce cytotoxic effects, including changes in cytoskeleton and nucleoskeleton in mouse spermatogonia cells, which may compromise the progression of spermatogenesis in a time- and dose-dependent manner.

A comprehensive toxicity study of zinc oxide nanoparticles versus their bulk in Wistar rats

2016 ◽  
Vol 35 (12) ◽  
pp. 1286-1304 ◽  
Author(s):  
Anurag Kumar Srivastav ◽  
Mahadeo Kumar ◽  
Nasreen Ghazi Ansari ◽  
Abhishek Kumar Jain ◽  
Jai Shankar ◽  
...  
Keyword(s):  
Zinc Oxide ◽  
Zinc Oxide Nanoparticles ◽  
Wistar Rats ◽  
Hematological Parameters ◽  
Control Group ◽  
Oxide Nanoparticles ◽  
Oral Toxicity ◽  
Zno Nps ◽  
Toxicological Effect ◽  
The Impact

The purpose of this study was to characterize the zinc oxide nanoparticles (ZnO-NPs) and their bulk counterpart in suspensions and to access the impact of their acute oral toxicity at doses of 300 and 2000 mg/kg in healthy female Wistar rats. The hematological, biochemical, and urine parameters were accessed at 24 and 48 h and 14 days posttreatment. The histopathological evaluations of tissues were also performed. The distribution of zinc content in liver, kidney, spleen, plasma, and excretory materials (feces and urine) at 24 and 48 h and 14 days posttreatment were accessed after a single exposure at dose of 2000 mg/kg body weight. The elevated level of alanine amino transferase, alkaline phosphatase, lactate dehydrogenase, and creatinine were observed in ZnO-NPs at a dose of 2000 mg/kg at all time points. There was a decrease in iron levels in all the treated groups at 24 h posttreatment as compared to control groups but returned to their normal level at 14 days posttreatment. The hematological parameters red blood cells, hemoglobin, hematocrit, platelets, and haptoglobin were reduced at 48 h posttreatment at a dose of 2000 mg/kg ZnO-NPs and showed hemolytic condition. All the treated groups were comparable to control group at the end of 14 days posttreatment. The zinc concentration in the kidney, liver, plasma, feces, and urine showed a significant increase in both groups as compared to control. This study explained that ZnO-NPs produced more toxicological effect as compared to their bulk particles as evidenced through alteration in some hemato-biochemical parameters and with few histopathological lesions in liver and kidney tissues.

The Anti-Breast Cancer Effects of Green-Synthesized Zinc Oxide Nanoparticles Using Carob Extract

2020 ◽  
Vol 20 ◽  
Author(s):  
Vahid Pouresmaeil ◽  
Shaghayegh Haghighi ◽  
Asieh S. Raeisalsadati ◽  
Ali Neamati ◽  
Masoud Homayouni-Tabrizi
Keyword(s):  
Breast Cancer ◽  
Zinc Oxide ◽  
Zinc Oxide Nanoparticles ◽  
Quantitative Polymerase Chain Reaction ◽  
Breast Cancer Cell Lines ◽  
Control Group ◽  
Oxide Nanoparticles ◽  
Dependent Manner ◽  
Zno Nps ◽  
Dose Dependent

Background: The use of nanoparticles synthesized by the green method to treat cancer is fairly recent. The aim of this study was to evaluate cytotoxicity, apoptotic and anti-angiogenic effects and their expression of involving genes, of zinc oxide nanoparticles (ZnO-NPs) synthesized with Carob extract on different human breast cancer cell lines. Methods: ZnO-NPs synthesized using the extract of Carob and characterized with various analytical techniques. The MCF-7 and MDA-MB231 cells were treated at different times and concentrations with ZnO-NPs. The cytotoxicity, apoptosis and anti-angiogenic were examined using a series of cellular assays. Expression of apoptotic genes (Bax and Bcl2) and anti-angiogenic genes, vascular endothelial growth factor (VEGF) and its receptor (VEGF-R) in cancer cells treated with ZnO-NPs were examined with Reverse transcription-quantitative polymerase chain reaction (RT-qPCR). The antioxidant activities of ZnO-NPs evaluated by ABTS and DPPH assay. Results: Exposure of cells to ZnO-NPs resulted in a dose-dependent loss of cell viability. The IC50 at 24, 48 and 72 hours were 125, 62.5 and 31.2µg/ml respectively (p<0.001). ZnO-NPs treated cells showed in fluorescent microscopy that ZnONPs are able to upregulate apoptosis and RT-qPCR revealed upregulation of Bax (p<0.001) and downregulation of Bcl-2 (p<0.05). ZnO-NPs increased VEGF gene expression while decreasing VEGF-R (p<0.001). The antioxidant effects of ZnO-NPs were higher than control group and were dose dependent manner (p<0.001). Conclusion: ZnO-NPs synthetized using Carob extract have the ability to eliminate breast cancerous cells and inhibit angiogenesis so could be used as anticancer agent.

scholarly journals Protective Role of Nrf2 in Zinc Oxide Nanoparticles-Induced Lung Inflammation in Female Mice and Sexual Dimorphism in Susceptibility

2022 ◽  
Author(s):  
Radwa Sehsah ◽  
Wenting Wu ◽  
Sahoko Ichihara ◽  
Naozumi Hashimoto ◽  
Cai Zong ◽  
...  
Keyword(s):  
Zinc Oxide ◽  
Proinflammatory Cytokines ◽  
Lung Inflammation ◽  
Zinc Oxide Nanoparticles ◽  
Synergistic Effects ◽  
Oxide Nanoparticles ◽  
Mrna Levels ◽  
Male Mice ◽  
Zno Nps ◽  
Female Mice

Abstract Background Zinc oxide nanoparticles (ZnO-NPs) are used in various products such as rubber, paint, and cosmetics. Our group reported recently that Nrf2 protein provides protection against ZnO-NPs-induced pulmonary inflammation in male mice. The present study investigated the effect of Nrf2 deletion on the lung inflammatory response in female mice exposed to ZnO-NPs. Methods Twenty-four female Nrf2−/− mice and the same number of female Nrf2+/+ mice were each divided into three equal groups and each exposed to ZnO-NPs at either 0, 10 or 30 µg/mouse by pharyngeal aspiration. Bronchoalveolar lavage fluid (BALF) and lungs were collected 14 days later to quantify protein level, number of inflammatory cells, and for scoring inflammation histopathologically. The mRNA levels of Nrf2-depedent antioxidant enzymes and proinflammatory cytokine in lung tissue were measured. Results Exposure to ZnO-NPs increased all types of BALF cells and lung inflammation scores in both of female Nrf2−/− and Nrf2+/+ mice, and Nrf2 deletion enhanced ZnO-NPs-induced increase in the number of eosinophils in BALF. Nrf2 deletion enhanced ZnO-NPs-induced downregulation of GR and upregulation of HO-1 and TNFα. Nrf2 deletion decreased mRNA levels of CAT, GcLc and NQO1 and increased that for GcLm and MT-2. ZnO-NPs dose-dependently increased the level of oxidized glutathione (GSSG), and mRNA levels of proinflammatory cytokines/chemokines; KC, MIP-2, IL-6, IL-1β and MCP-1 only in wild-type mice, and Nrf2 deletion decreased total glutathione levels and upregulated the above proinflammatory cytokines/chemokines regardless of level of exposure to ZnO-NPs. Taken together with our previous results in male mice, our results showed a lower susceptibility of females to lung inflammation, relative to males, irrespective of Nrf2 deletion, and that enhancement of ZnO-NPs-induced upregulation of HO-1 and TNFα and downregulation of GR by deletion of Nrf2 is specific to female mice. Conclusion We conclude that Nrf2 provides protection in female mice against increase in BALF eosinophils, probably through down-regulation of proinflammatory cytokines/chemokines and upregulation of oxidative stress-related genes. The study also suggests lower susceptibility to lung inflammation in female mice relative to their male counterparts and the synergistic effects of sex and exposure to ZnO-NPs on mRNA expression of GR, HO-1 or TNFα.

scholarly journals Histopathological Effect of Zinc Oxide Nanoparticles on Kkidney and Liver Tissues in Albino Male Mice

2018 ◽  
Vol 31 (1) ◽  
pp. 9 ◽  
Author(s):  
Ruqayah Ali Salman
Keyword(s):  
Zinc Oxide ◽  
Zinc Oxide Nanoparticles ◽  
Histopathological Examination ◽  
Control Group ◽  
Oxide Nanoparticles ◽  
Male Mice ◽  
Zno Nps ◽  
Liver And Kidney ◽  
Dose Levels ◽  
Liver Tissues

    Objective: The present study investigated the effects of different dose levels of Zinc oxide Nanoparticles (ZnO NPs) on the liver and kidney tissues in albino male mice. Methadology: ZnO NPs was administrated as a daily oral dose of (150, 350 mg/kg body weight) gavage for 2 weeks. Eighteen male mices were used by dividing them into three groups. Result: Histopathological examination of kidney and hepatic tissues treated with ZnO NPs showed toxicity changes compared with control group. Conclusion:This study demonstrated the ability of ZnO NPs to affect on kidney and liver tissues. Recommendation: More study needed to know the effect of different doses of nanoparticles on human health.  

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