Effects of Orlistat and Herbal Mixture Extract on Renal Function and Oxidative Stress Biomarkers in a Rat Model of High Fat Diet

The purpose of this work was to evaluate the effect of dietary carotenoids from spinach on the inflammation and oxidative stress biomarkers, liver lipid profile, and liver transcriptomic and metabolomics profiles in Sprague–Dawley rats with steatosis induced by a high-fat diet. Two concentrations of spinach powder (2.5 and 5%) were used in two types of diet: high-fat (H) and standard (N). Although rats fed diet H showed an accumulation of fat in hepatocytes, they did not show differences in the values of adiponectin, tumor necrosis factor alpha (TNF-α), and oxygen radical absorption (ORAC) in plasma or of isoprostanes in urine compared with animals fed diet N. The consumption of spinach and the accumulation of α and β carotenes and lutein in the liver was inversely correlated with serum total cholesterol and glucose and the content of hepatic cholesterol, increasing monounsaturated fatty acids (MUFA), polyunsaturated fatty acids (PUFA) and reducing cholesterol in the livers of rats fed diet H and spinach. In addition, changes in the expression of genes related to the fatty liver condition occurred, and the expression of genes involved in the metabolism of fatty acids and cholesterol increased, mainly through the overexpression of peroxisome proliferator activated receptors (PPARs). Related to liver metabolites, animals fed with diet H showed hypoaminoacidemia, mainly for the glucogenic aminoacids. Although no changes were observed in inflammation and oxidative stress biomarkers, the consumption of spinach modulated the lipid metabolism in liver, which must be taken into consideration during the dietary treatment of steatosis.

Download Full-text

The effect of cilostazol on hippocampal memory and oxidative stress biomarkers in rat model of diabetes mellitus

Brain Research ◽

10.1016/j.brainres.2019.03.025 ◽

2019 ◽

Vol 1715 ◽

pp. 182-187 ◽

Cited By ~ 3

Author(s):

Abeer M. Rababa'h ◽

Afrah N. Mardini ◽

Karem H. Alzoubi ◽

Mera A. Ababneh ◽

Rabaa Y. Athamneh

Keyword(s):

Oxidative Stress ◽

Diabetes Mellitus ◽

Rat Model ◽

Oxidative Stress Biomarkers ◽

Stress Biomarkers ◽

And Oxidative Stress

Download Full-text

Galangin Resolves Cardiometabolic Disorders Through Modulation of AdipoR1/COX-2/ NF-κB Expressions in High-Fat Diet Fed Rats

10.21203/rs.3.rs-409636/v1 ◽

2021 ◽

Author(s):

Patoomporn Prasatthong ◽

Sariya Meephat ◽

Siwayu Rattanakanokchai ◽

Juthamas Khamseekaew ◽

Sarawoot Bunbupha ◽

...

Keyword(s):

Oxidative Stress ◽

High Fat Diet ◽

Biological Activities ◽

Metformin Treatment ◽

High Fat ◽

Oxidative Stress Biomarkers ◽

Impaired Liver Function ◽

Cardiometabolic Disorders ◽

Cox 2 ◽

And Oxidative Stress

Abstract Background: Rats fed with a high-fat diet exerts signs of cardiometabolic disorders. Galangin is a natural flavonoid mainly isolated from honey and Alpinia officinarum Hance and has various biological activities. This study evaluated whether galangin could alleviate cardiometabolic disorders, inflammation and oxidative stress in a high-fat diet fed rats. Methods: Male Sprague-Dawley rats were fed with a high-fat diet plus 15% fructose in drinking water for 4 months to induced signs of metabolic syndrome (MS), and they were treated with galangin at a dose 25 or 50 mg/kg or metformin at a dose 100 mg/kg or vehicle for the last four weeks. All data were expressed as mean ± S.E.M. Data were analyzed by one-way analysis of variance followed by Tukey’s post-hoc test for multiple comparisons analysis.Results: Rats fed with a high-fat diet had impaired glucose tolerance, insulin resistance, hyperglycemia, hypertrophy of adipocytes, impaired liver function and hypertension. These signs of MS were alleviated by galangin or metformin treatment (p<0.05). Galangin or metformin alleviated cardiac dysfunction and remodeling induced by a high-fat diet in rats (p<0.05). Tumor necrosis factor-α and interleukin-6 concentrations and expression were high in plasma and cardiac tissue in MS rats, and these inflammatory markers were suppressed by galangin or metformin treatment (p<0.05). Galangin alleviated a high-fat diet induced low levels of adiponectin in rats. Galangin or metformin decreased oxidative stress biomarkers, aortic superoxide generation and plasma and cardiac MDA levels, and raised endogenous antioxidant enzume activities, catalase, and superoxide dismutase, in MS rats (p<0.05). Downregulation of adiponectin receptor1 (AdipoR1) and cyclooxygenase-2 (COX-2) as well as upregulation of nuclear factor kappa B (NF-κB) expression were observed in MS rats. These alterations of protein expressions were recovered in MS rats treated with galangin or metformin. Conclusions: Galangin reduced cardiometabolic disorders in high-fat diet induced MS rats. The underlying mechanisms might be relevant to suppression of inflammation and oxidative stress and restoration of AdipoR1/COX-2/NF-κB expression.

Download Full-text

Protective Effects of Orlistat on Lipid Profile, Cardiac Oxidative Stress Biomarkers and Histology in High-fat Diet-induced Obese Rats

IIUM Medical Journal Malaysia ◽

10.31436/imjm.v18i2.101 ◽

2020 ◽

Vol 18 (2) ◽

Author(s):

Othman ZA ◽

Wan ghazali WS ◽

Noordin L ◽

Omar N ◽

Mohd. Yusof NA ◽

...

Keyword(s):

Oxidative Stress ◽

High Fat Diet ◽

Male Rats ◽

Protective Effects ◽

Sprague Dawley ◽

High Fat ◽

Oxidative Stress Biomarkers ◽

Stress Biomarkers ◽

Obesity Index ◽

Obese Rats

Introduction: Orlistat is a widely used drug in treating obesity as it promotes weight reduction. The aim of this study was to determine the protective effects of orlistat (10 mg/kg/day) on cardiovascular parameters and oxidative stress biomarkers in high-fat diet (HFD)-induced obese rats. Methods: Twenty-four male rats Sprague Dawley rats were divided into three groups and fed with normal diet (N), HFD and HFD with orlistat (HFD+O). Orlistat was administered daily by oral gavage and after six weeks, all rats were sacrificed. Results: Administration of orlistat along with HFD (HFD+O) has brought significant decreases in Lee obesity index and LDL level compared to HFD group. Activities of cardiac superoxide dismutase (SOD), glutathione peroxidase (GPx) and catalase (CAT) were significantly higher, whereas level of oxidised LDL was significantly lower in HFD+O group compared to HFD group. HFD group had significantly higher necrotic patch area in myocardium while minimal histological changes were seen in HFD+O group. Conclusion: This study may suggest that administration of orlistat at 10 mg/kg/day for 6 weeks may have protective effects against the changes on Lee obesity index, lipid profiles, cardiac oxidative stress biomarkers and histology of myocardium in HFD-induced obese rats possibly through its hypolipidaemic and antioxidant actions.

Download Full-text