Anti-hyperglycemic Efficiency of the Aqueous Seed Extract of Mucuna pruriens in Nicotinamide-Streptozotocin-induced Diabetic Wistar Rats

This study was undertaken to evaluate the remedial effect of the aqueous seed extract of Mucuna pruriens (ASEMP) on the endocrine region of pancreas of nicotinamide-streptozotocin-induced diabetes in Wistar rats. The anti-hyperglycemic efficiency of two varieties of Mucuna pruriens var utilis; IIHR Selection 3 and Arka Dhanvantari was investigated. Oral administration of ASEMP against the nicotinamide-streptozotocin-induced diabetes in the Wistar rats showed anti-hyperglycemic effect on the blood glucose level (94 ± 0.2 mg/dl ASEMP 400 mg/kg) when compared with the control group (92 ± 0.2 mg/dl glibenclamide 5 mg/kg), (248 ± 0.3 mg/dl Diabetic control) and (90 ± 0.3 mg/dl Normal control). Significant reduction in creatinine level of the nicotinamide-streptozotocin-induced diabetic rats treated with ASEMP was also recorded. Histopathology examination of the endocrine region of pancreas of the rats revealed restoration of pancreatic islet cells in the diabetic-ASEMP treated rats as the beta cell mass increased and necrotic changes was reduced significantly in contrast with the diabetic control group which showed degenerated pancreatic islet cells. Therefore, this study supports and recommends the exploration of the aqueous seed extract of M. pruriens as an alternative medicine for the management of type-2 diabetes.

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Effect of sulfur dioxide exposure on histopathology and morphometry of pancreatic islet cells and glycemic indices in Wistar rats

Environmental Science and Pollution Research ◽

10.1007/s11356-021-12672-9 ◽

2021 ◽

Author(s):

Razieh Musapour Soltan-Abad ◽

Sorayya Kheirouri ◽

Mehran Mesgari Abbasi ◽

Mohammad Alizadeh ◽

Monireh Khordadmehr

Keyword(s):

Sulfur Dioxide ◽

Pancreatic Islet ◽

Wistar Rats ◽

Islet Cells ◽

Pancreatic Islet Cells ◽

Glycemic Indices

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Effect of Abelmoschus esculentus (okra)-based diet on streptozotocin-induced diabetes mellitus in adult Wistar rats

Tropical Journal of Pharmaceutical Research ◽

10.4314/tjpr.v19i8.24 ◽

2020 ◽

Vol 19 (8) ◽

pp. 1737-1743

Author(s):

Patrick O. Uadia ◽

Isaac O. Imagbovomwan ◽

Kelly Oriakhi ◽

Ikechi G. Eze

Keyword(s):

Diabetes Mellitus ◽

Normal Control ◽

Wistar Rats ◽

Diabetic Control ◽

Normal Diet ◽

Abelmoschus Esculentus ◽

Control Group ◽

Β Cells ◽

Streptozotocin Induced Diabetes ◽

Ad Libitum

Purpose: To evaluate the effects of an okra-based diet on streptozotocin-induced diabetes mellitus in adult Wistar rats and its mechanism of action.Methods: Wistar rats (6) were administered streptozotocin (50 mg/kg ip) after an overnight fast. Upon confirmation of diabetes mellitus, the animals were fed ad libitum for 21 days with formulated okrabased test diet in place of normal diet. The rats treated similarly with streptozotocin and fed ad libitum with the normal diet served as diabetic control while rats fed on normal diet and not treated with streptozotocin served as the negative control. Thereafter, the rats were sacrificed, fasting bloodcollected and analysed for glucose concentration and biochemical parameters. Pancreas was also excised for histopathological studies.Results: There was a significant increase in body weight, HDL-cholesterol (p< 0.05) but significant decrease in blood glucose (p < 0.05), serum total cholesterol, triglyceride, LDL-cholesterol and VLDLcholesterol concentrations in the okra-fed diabetic treated rats when compared to the diabetic control group. Furthermore, superoxide dismutase activity (SOD) was significantly higher in the diabetic control, and reduced significantly when fed with okra-based diet (p < 0.05). Catalase (CAT) activity was significantly (p < 0.05) decreased in diabetic control and treated group, whereas it was significantly (p < 0.05) increased in normal control rats. There was a significant (p < 0.05) decrease in reduced glutathione levels. The significant (p< 0.05) increase in malondialdehyde in the diabetic group was significantly decrease in the diabetic rats fed with okra-diet. Also serum insulin level was significantly (p < 0.05) increased and serum α amylase activity was significantly (p < 0.05) decrease in the diabetic treated rats. Histology results show that there was damage to the β cells of the pancreas in the diabetic control when compared to normal control, but rats fed okra diet was able to regenerate endocrine β cells.Conclusion: Okra-based diet lowers hyperglycaemia as well as regenerate/repair endocrine β-cells and exocrine tissues of the pancreas damage by streptozotocin Keywords: Abelmoschus esculentus, Diabetes mellitus, anti-diabetic, Insulin, α-amylase, Mechanism

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Antioxidant activity of chito-oligosaccharides on pancreatic islet cells in streptozotocin-induced diabetes in rats

World Journal of Gastroenterology ◽

10.3748/wjg.15.1339 ◽

2009 ◽

Vol 15 (11) ◽

pp. 1339 ◽

Cited By ~ 42

Author(s):

Wen-Peng Yuan ◽

Bing Liu ◽

Chang-Heng Liu ◽

Xiao-Jun Wang ◽

Mian-Song Zhang ◽

...

Keyword(s):

Antioxidant Activity ◽

Pancreatic Islet ◽

Islet Cells ◽

Pancreatic Islet Cells ◽

Streptozotocin Induced Diabetes

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Effects Of Annona Muricata (Linn) On The Morphology Of Pancreatic Islet Cells Of Experimentally-Induced Diabetic Wistar Rats

The Internet Journal of Alternative Medicine ◽

10.5580/229c ◽

2008 ◽

Vol 5 (2) ◽

Keyword(s):

Pancreatic Islet ◽

Wistar Rats ◽

Islet Cells ◽

Annona Muricata ◽

Pancreatic Islet Cells ◽

Experimentally Induced

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The Role of Mesenchymal Stem Cells in Regulating PDGF and VEGF during Pancreatic Islet Cells Regeneration in Diabetic Animal Model

Folia Medica ◽

10.3897/folmed.63.e57636 ◽

2021 ◽

Vol 63 (6) ◽

pp. 875-883

Author(s):

Agung Putra ◽

Zakariya Hadi Suwiryo ◽

Adi Muradi Muhar ◽

Agus Widyatmoko ◽

Fifin Luthfia Rahmi

Keyword(s):

Stem Cells ◽

Mesenchymal Stem Cells ◽

Blood Glucose ◽

Pancreatic Islet ◽

Islet Cells ◽

Pancreatic Tissue ◽

Enzyme Linked Immunosorbent Assay ◽

Control Group ◽

Prolonged Release ◽

Pancreatic Islet Cells

Introduction: Diabetes is a heterogeneous group of metabolic diseases characterized by elevated blood glucose due to autoimmune disorder or a combination of insulin resistance and insulin deficiency. VEGF and PDGF are the main actors in the regeneration of damaged pancreatic tissue. However, the prolonged release of these molecules may induce fibrosis formation. Mesenchymal stem cells (MSCs) have a high potential to regenerate damaged pancreatic tissue by releasing PDGF and VEGF. Aim: This study aimed to investigate the effect of MSCs on the levels of PDGF and VEGF on days 2 and 44 in diabetic mice and determine the number of pancreatic islet cells and blood glucose levels. Materials and methods: This study used a post-control group design with animals divided into five groups: sham, control, and three treatment groups (P) which were given MSCs at doses of 1.5×105, 3×105, and 6×105 cells. The levels of PDGF, VEGF, and blood glucose were measured by enzyme-linked immunosorbent assay (ELISA), while the number of pancreatic islet cells was analyzed using H&E staining. Results: This study showed a significant increase of VEGF and PDGF levels on day 2 and a significant increase in islet cell percentages on day 44 in line with the decreased blood glucose level. However, there was no difference between VEGF and PDGF levels on day 44. Conclusions: MSCs regulate PDGF and VEGF levels in wound healing phases and remodel pancreatic islet β-cells regeneration to control blood glucose in diabetic model mice.

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CLERODENDRUM VOLUBILE ETHANOL LEAF EXTRACT: AN EFFECTIVE MITIGATOR AGAINST DOXORUBICIN-INDUCED HEPATORENAL TOXICITIES IN RATS

Open Journal of Bioscience Research (ISSN: 2734-2069) ◽

10.52417/ojbr.v2i1.189 ◽

2021 ◽

Vol 2 (1) ◽

pp. 01-09

Author(s):

O. B. Akinsanya ◽

P. F. Ayodele ◽

O. F. Onifade ◽

M. O. Salimom

Keyword(s):

Diabetes Mellitus ◽

Beta Cells ◽

Pancreatic Islet ◽

Islet Cells ◽

Diabetic Rats ◽

Persea Americana ◽

Control Group ◽

Albino Rats ◽

Pancreatic Islet Cells ◽

Standard Drug

Diabetes mellitus develops either due to insufficient insulin secretion or lack of insulin resulting from damaged pancreas beta cells. Dyslipidaemia is an acute complication in diabetes mellitus. The aim of this study was to investigate the extenuating effects of T. cattapa leaves and P. americana seed aqueous extracts on streptozotocin-induced lipids profile perturbation and pancreatic damage in experimental rats. Thirty male albino rats were randomly divided into six groups (n = 5). Group 1: (negative control), group 2: 80 mg/kgbwt streptozotocin (positive control), group 3: (80 mg/kgbwt streptozotocin + 200 mg/kgbwt T. catappa leave extract), group 4: (80 mg/kgbwt streptozotocin + 200 mg/kgbwt Persea americana seed extract), group 5: (80 mg/kgbwt streptozotocin + 200 mg/kgbwt extracts-mixture), group 6: (80 mg/kgbwt streptozotocin + 5 mg/kgbwt glibenclamide (standard drug). A single dose of streptozotocin was administered to the rats intraperitoneally; the extracts and glibenclamide were administered orally for 21 days, after which their pancreas was excised for histology. Thereafter, extracts of T. catappa leaves and P. americana seed on serum lipid levels imbalance in streptozotocin-induced diabetic rats were investigated. Histological studies showed a degenerative effect of the pancreatic islet cells of strotozotocin-induced groups. Results showed improvement in the regulation of lipid metabolism as well as the regeneration of the beta cells of pancreas on treatment with T. catappa leaves and P. americana seed. The extracts both equally exert significant antidyslipidemic effects in diabetic rats, in view of the extenuating effects of the extracts on pancreatic islet cells.

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In Vivo ZIMIR Imaging of Mouse Pancreatic Islet Cells Shows Oscillatory Insulin Secretion

Frontiers in Endocrinology ◽

10.3389/fendo.2021.613964 ◽

2021 ◽

Vol 12 ◽

Cited By ~ 1

Author(s):

Shiuhwei Chen ◽

ZhiJiang Huang ◽

Harrison Kidd ◽

Min Kim ◽

Eul Hyun Suh ◽

...

Keyword(s):

Insulin Secretion ◽

Pancreatic Islet ◽

Islet Cells ◽

Cell Mass ◽

Beta Cell Mass ◽

Pancreatic Islet Cells ◽

Systemic Delivery ◽

Β Cells ◽

Spatiotemporal Resolution

Appropriate insulin secretion is essential for maintaining euglycemia, and impairment or loss of insulin release represents a causal event leading to diabetes. There have been extensive efforts of studying insulin secretion and its regulation using a variety of biological preparations, yet it remains challenging to monitor the dynamics of insulin secretion at the cellular level in the intact pancreas of living animals, where islet cells are supplied with physiological blood circulation and oxygenation, nerve innervation, and tissue support of surrounding exocrine cells. Herein we presented our pilot efforts of ZIMIR imaging in pancreatic islet cells in a living mouse. The imaging tracked insulin/Zn2+ release of individual islet β-cells in the intact pancreas with high spatiotemporal resolution, revealing a rhythmic secretion activity that appeared to be synchronized among islet β-cells. To facilitate probe delivery to islet cells, we also developed a chemogenetic approach by expressing the HaloTag protein on the cell surface. Finally, we demonstrated the application of a fluorescent granule zinc indicator, ZIGIR, as a selective and efficient islet cell marker in living animals through systemic delivery. We expect future optimization and integration of these approaches would enable longitudinal tracking of beta cell mass and function in vivo by optical imaging.

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