scholarly journals Circulating HBV Genotypes among Animal and Non-Animal Handlers in Osun State, Nigeria

2020 ◽  
pp. 13-21
Author(s):  
I. O. Okonko ◽  
I. R. Gidado ◽  
B. U. Anomneze ◽  
O. O. Opaleye
Keyword(s):  
Molecular Analysis ◽  
Cross Sectional Study ◽  
Hbv Dna ◽  
Hbv Infection ◽  
Hbv Genotype ◽  
Cross Sectional ◽  
Hbv Genotypes ◽  
Genotype A ◽  
Genotype E ◽  
Animal Handlers

Aim: Hepatitis B virus (HBV) is not uncommon among animal and non-animal handlers. The brutality of HBV infection and the outcome of treatment is linked with exact HBV genotypes. No study on the circulation of HBV genotypes has been reported among animal and non-animal handlers in Nigeria. This study was intended to evaluate the genotypic distribution among animal and non-animal handlers in Osun State, Nigeria. Study Design:  Cross-sectional study. Place and Duration of Study: Ladoke Akintola University of Technology (LAUTECH), Nigeria, between June 2015 and July 2019. Methods: Blood samples were obtained from HBsAg positive individuals and screened for HBV-DNA from cohorts of animal and non-animal handlers. HBV-DNA was extracted, amplified and genotyped using a multiplex PCR technique with primers specific for six genotypes of HBV (Genotype A, B, C, D, E and F). Results: Results showed that a total of 11 (6.1%) of the 180 animal and non-animal handlers evaluated were positive to HBsAg and 4.4% were positive for HBV-DNA by a semi-nested PCR using HBV specific primer pairs. The molecular analysis of the sera of 11 HBsAg positive animal and non-animal handlers showed that 72.7% of them had a true HBV infection. Results further show that genotype E (75.0%) was predominant over genotype A (12.5%) and mix genotypes (D and E) with 12.5% prevalence. Other genotypes were not detected. Of the 8 positive HBV-DNA samples, 7 (87.5%) were males and one (12.5%) was a female. All animal and non-animal handlers with true HBV infection were found to harbour HBV genotype E predominantly. Conclusion: The molecular analysis of HBV-DNA and genotypes circulating among animal and non-animal handlers shows that the majority of the subjects with true HBV infection were found to predominantly harbour HBV genotype E in Osun state, Nigeria. The study further highlights the predominance HBV genotype E in Nigeria.

scholarly journals Molecular Diagnosis in Differentiating Active and Inactive Forms of Hepatitis B Virus Carriers

2018 ◽  
Vol 26 (10) ◽  
pp. 41-45
Author(s):  
Salah Tofik Jalal Balaky ◽  
Saeed Ghulam Hussain ◽  
Amer Ali Khaleel ◽  
Furat Tahseen Sabeer ◽  
Ahang Hasan Mawlood
Keyword(s):  
Nucleic Acid ◽  
Hepatitis B ◽  
Cross Sectional Study ◽  
Hbv Dna ◽  
Hbv Infection ◽  
Detection Methods ◽  
Rt Pcr ◽  
Cross Sectional ◽  
Blood Samples ◽  
Acid Test

Background & objectives: Introducing a nucleic acid test program is aimed to diagnose and reduces the risk of viral infection or transmission. DNA assay for HBV can detect infection in the windows period, chronic occult infection and can discriminate between active and inactive HBV infection. This cross-sectional study designed to diagnose, analyze HBV infection and to differentiate active from inactive infection based on viral DNA detection. Methods: Blood samples were collected from 256 patients previously diagnosed on the clinical ground as hepatitis B seropositive in Erbil Central Lab. The viral nucleic acid quantitative assessment was done for the collected samples using RT-PCR. Q-square was performed for statistical analysis. Results: Out of 256 collected blood samples 93 (36.3%) showed HBV-DNA positive titers above 50 IU/ml. Among positive subjects, 67 (72.04%) was categorized as inactive carriers (˂ 2000-20.000 IU/ml HBV-DNA titers). Conclusions: The data produced from this study confirmed the importance of the RT-PCR technique in sensitivity and reliability as a superior diagnostics tool specifically in differentiating active from inactive HBV carriers.

scholarly journals Prevalence of HBV-genotypes in immigrants affected by HBV-related chronic active hepatitis

2007 ◽  
Vol 44 (1) ◽  
pp. 54-57 ◽  
Author(s):  
Emilio Palumbo ◽  
Gaetano Scotto ◽  
Giuseppina Faleo ◽  
Donatella Concetta Cibelli ◽  
Annalisa Saracino ◽  
...  
Keyword(s):  
Antiviral Treatment ◽  
Hbv Dna ◽  
Hbv Infection ◽  
Genotype Distribution ◽  
Hbv Genotype ◽  
Hbv Genotypes ◽  
Sub Saharan ◽  
Normal Alt ◽  
Different Response ◽  
Detectable Serum

BACKGROUND: The genetic heterogeneity of the HBV genome has been established and eight genotypes can be classified according to the criterion of >8% differences in the complete nucleotide sequence of the viral genome. AIMS: To evaluate the prevalence of HBV-infection in a population of immigrants and to determine in patients with detectable serum HBV-DNA the HBV-genotypes. METHODS: Between January 2005 and December 2005 a total of 556 immigrants were tested for HBsAg. In HBsAg positive patients the biochemical and virological activity of infection and the possible presence of co-infections (HCV, HDV, HIV) were evaluated. In patients with detectable serum HBV DNA, the HBV-genotype was determined by INNOLiPA. RESULTS: Among the 556 subjects tested, 60 (10.7%) resulted HBsAg positive. All were men, and 42 (70%) come from Africa, 10 (16.6%) from Asia and 9 (14.4%) from East-Europe. 28/60 (46.6%) patients presented normal ALT levels (<40 IU/L) and undetectable serum HBV DNA (<100 copies/mL in real-time PCR), while 32 (53.4%) patients had ALT levels above laboratory normal values and detectable serum HBV DNA. Genotype distribution was as follow: genotype E, 16 (50%), genotype D, 9 (28.1%), genotype A, 7 (21.9%). CONCLUSION: Our study evidences a moderate prevalence of HBV-infection in immigrants, particularly in sub-Saharan African people, and the potentiality of migratory flow in the introduction of genotype non-D hepatitis B virus, potentially characterized by a different natural history and, possibly, a different response to antiviral treatment.

scholarly journals Prevalence of HCV and/or HBV coinfection in Iranian HIV-infected patients

2020 ◽  
Author(s):  
Farzaneh Dehghani-Dehej ◽  
Zinat Hosseini ◽  
Poupak Mortazkar ◽  
Khadijeh Khanaliha ◽  
Maryam Esghaei ◽  
...  
Keyword(s):  
Hepatitis B ◽  
Hepatitis B Surface Antigen ◽  
Cross Sectional Study ◽  
Hbv Dna ◽  
Hcv Rna ◽  
Hepatitis B E Antigen ◽  
Hbv Genotype ◽  
Cross Sectional ◽  
Hiv Rna ◽  
Hcv Coinfection

Aim: HIV-infected patients risk coinfection with HBV and HCV. This study aimed to investigate molecular epidemiology of HBV and HCV coinfection in Iranian HIV-infected individuals. Materials & methods: In this cross-sectional study, serological markers of HBV and HCV infection (hepatitis B surface antigen [HBsAg], hepatitis B e-antigen [HBeAg], hepatitis B e-antibody [HBeAb] and hepatitis B core antibody [HBcAb]) and anti-HCV antibodies [anti-HCV Abs] were tested in 198 Iranian HIV-infected patients. From plasma, HBV viral load was determined using COBAS TaqMan 48, and HCV-RNA was detected by reverse transcriptase-nested PCR. Results: 85 out of 198 (42.9%) patients were anti-HCV Ab positive and 42/198 (21.2%) had detectable HCV-RNA. Eight (4.0%) had traceable HBV-DNA. All these patients were infected by HBV genotype D. 55 (27.8%) were HBcAb positive. Nine (4.4%) were HBsAg and anti-HCV Ab positive. Conclusion: None were HIV-RNA/HCV-RNA/HBV-DNA positive, 21.2% were HIV-RNA/HCV-RNA positive and 4.0% were HIV-RNA/HBV-DNA positive. Therefore, studies on diagnosing these infections in HIV-infected individuals may be valuable.

scholarly journals Seroprevalence of Malaria and Hepatitis B Coinfection among Pregnant Women in Tamale Metropolis of Ghana: A Cross-Sectional Study

2018 ◽  
Vol 2018 ◽  
pp. 1-12 ◽  
Author(s):  
Gideon Kofi Helegbe ◽  
Paul Armah Aryee ◽  
Baba Sulemana Mohammed ◽  
Anthony Wemakor ◽  
David Kolbila ◽  
...  
Keyword(s):  
Hepatitis B ◽  
Hiv Infection ◽  
Pregnant Women ◽  
Cross Sectional Study ◽  
Hbv Infection ◽  
Full Blood Count ◽  
Cross Sectional ◽  
Tamale Metropolis ◽  
The Mean ◽  
Glucose 6 Phosphate Dehydrogenase

Background. Coinfections are becoming common risk factors that may contribute to the increased burden of morbidity in pregnancy. The aim of this study was to assess the seroprevalence of coinfections of malaria, hepatitis B (HBV), human immunodeficiency virus (HIV), and syphilis among pregnant women attending antenatal clinics (ANC) in the Tamale Metropolis. Methods. By means of rapid diagnostic tests (RDTs), pregnant women attending the Tamale Teaching Hospital (TTH) were screened for malaria, HBV infection, HIV infection, and syphilis from March 2013 to February 2015. Haemoglobin (Hb) values, sickling, and glucose-6-phosphate dehydrogenase deficiency (G6PDd) statuses were also assessed using full blood count (FBC), sodium metabisulphite, and methaemoglobin reduction tests, respectively. Logistic regression analysis was performed to estimate the risks/odds ratios (ORs) for the coinfections and other variables (age, gravidity, and time of the first ANC visit) with 95% confidence intervals (CIs) and set p values for accepting any differences at <0.05. Results. Within the two-year study period, data were collected from 3,127 pregnant women. The mean age (SD) of the pregnant women was 28.5 (±5.0) years. Of the total number, seroprevalence was high for malaria (11.6%) and HBV infection (4.2%) and low for HIV infection (1.0%) and syphilis (0.4%) monoinfections. Mal/HBV coinfection was higher (0.7%) when compared with Mal/HIV (0.1%), Mal/syphilis (0.0%), HBV/HIV (0.0%), HBV/syphilis (0.1%), and HIV/syphilis (0.0%) coinfections. The mean Hb (g/dl) for the women with the four monoinfections was significantly different from one another (p=0.009). Pregnant women with malaria infection were about 2 times more likely to be coinfected with HBV even after adjusting for potential confounders (adjusted odds ratio (AOR) = 1.66, 95% CI = 1.04–2.65, p=0.031). Those in their third trimester and visiting the ANC for the first time were significantly less likely to be infected with HBV (AOR = 0.45, 95% CI = 0.28–0.73, p=0.001), with malaria/HBV coinfection (AOR = 0.09, 95% CI = 0.01–0.68, p=0.020), and with any coinfection (AOR = 0.19, 95% CI = 0.06–0.63, p=0.007). Conclusion. A comparatively high seroprevalence of malaria and its coinfection with HBV in pregnant women was observed in this study. Considering the effects that both malaria and HBV have on the liver, it would be expedient to conduct further studies to assess liver function among malaria/HBV-infected individuals, while interventions to prevent coinfections among pregnant women are intensified.

How to evaluate the HbeAg-positive during the natural course of Chronic Hepatitis B in clinic? A cross-sectional study

2019 ◽  
Author(s):  
JinLi Zheng ◽  
Yang Huang ◽  
Lingpeng Yang ◽  
Li Jiang
Keyword(s):  
Cross Sectional Study ◽  
Hbv Dna ◽  
The Other ◽  
Independent Factor ◽  
Negative Group ◽  
Sectional Study ◽  
Cross Sectional ◽  
Positive Group ◽  
Independent Risk Factors ◽  
Low Levels

Abstract Background The previous studies showed the correlation between HBsAg and serum HBV DNA levels was weak or missing. Objective The study aims to investigate the correlation between HBeAg and HBV DNA levels, and to find an alternative tool to evaluate the HBV DNA level for clinicians. Methods A total of 1020 patients with CHB were enrolled in this cross-sectional study. We divided the patients into four groups as: HBeAg positivity and negativity groups, and high and low HBV DNA levels groups. Further, as per the levels of serum HBV DNA, we performed subgroups’ analyses for the HBeAg-positive and HBeAg-negative groups. Results Results showed that the ALT, ALB and HBeAg are independent factors to estimate the serum HBV DNA in CHB patients. But diagnosing the high levels of HBV DNA is not credible (the AUC=0.622, Fig1-A). In HBeAg-positive group, when the level of HBeAg is higher than 16.15 S/CO, we can predict the patient with high levels of HBV DNA (> 2000 IU/ml, AUC=0.787, Fig1-C) and the patients were 4 folds to have the high levels of HBV DNA than the HBeAg-negativ (table3). The levels of ALT and TB are the independent risk factors for the patients in HBeAg-negative group. When the levels of ALT and TB are higher 36.5 IU/L and 11.15 umol/L,respectively, the patient would have a high levels of HBV DNA (> 2000 IU/ml, AUC=0.609, Fig2-B). Conclusion HBeAg is an independent factor that reflects the levels of serum HBV DNA with a strong correlation, but it is not accurate to evaluate the levels of serum HBV DNA by the HBeAg-positive. On the other hand, the patients with HBeAg-negative are not mean having a low levels of HBV DNA, which can be evaluated by the levels of ALT and TB.

scholarly journals Cross-Protection of Hepatitis B Vaccination among Different Genotypes

Vaccines ◽  
2020 ◽  
Vol 8 (3) ◽  
pp. 456
Author(s):  
Takako Inoue ◽  
Yasuhito Tanaka
Keyword(s):  
Hepatitis B ◽  
Cross Protection ◽  
Hbv Infection ◽  
Immune Memory ◽  
Hepatitis B Vaccination ◽  
Infection Model ◽  
Hbv Genotype ◽  
Hb Vaccine ◽  
Genotype A

Hepatitis B (HB) vaccination is the most effective method for preventing HB virus (HBV) infection. Universal HB vaccination containing recombinant HB surface antigens (HBsAg) is recommended. Our data revealed that human monoclonal HB surface antibody (anti-HBs) from individuals inoculated with genotype C-based HB vaccine induced cross-protection against HBV genotype A infection. An in vitro infection model demonstrated anti-HBs-positive sera from individuals inoculated with genotype A- or C-based HB vaccine harbored polyclonal anti-HBs that could bind to non-vaccinated genotype HBV. However, because there were low titers of anti-HBs specific for HBsAg of non-vaccinated genotype, high anti-HBs titers would be required to prevent non-vaccinated genotype HBV infection. Clinically, the 2015 Centers for Disease Control and Prevention guidelines state that periodic monitoring of anti-HBs levels after routine HB vaccination is not needed and that booster doses of HB vaccine are not recommended. However, the American Red Cross suggests that HB-vaccine-induced immune memory might be limited; although HB vaccination can prevent clinical liver injury (hepatitis), subclinical HBV infections of non-vaccinated genotypes resulting in detectable HB core antibody could not be completely prevented. Therefore, monitoring anti-HBs levels after routine vaccination might be necessary for certain subjects in high-risk groups.

scholarly journals Self-reported selected zoonotic diseases among animal handlers in Urban Ahmedabad, India

2019 ◽  
Vol 12 (1) ◽  
pp. 176-182 ◽  
Author(s):  
Krupali Patel ◽  
Deepak Saxena
Keyword(s):  
Urban Areas ◽  
Family Members ◽  
Raw Milk ◽  
Cross Sectional Study ◽  
Zoonotic Diseases ◽  
Microbial Pathogens ◽  
Cross Sectional ◽  
Preventive Practices ◽  
Animal Handling ◽  
Animal Handlers

Background and Aim: Out of all global microbial pathogens, 61% are zoonoses. Zoonotic diseases (Z/D/S) are responsible for a large burden on the public health, livestock economies, and wildlife of India. Data on burden and knowledge about Z/D/S among animal handlers are limited for urban and peri-urban areas of India. The present study aimed to estimate the prevalence of self-reported selected Z/D/S and knowledge about those diseases among animal handlers in the urban area of Ahmedabad city, India. Materials and Methods: This cross-sectional study was conducted among 170 animal handlers from three zones of Ahmedabad city, India, from February to May 2017. Data were collected on sociodemographic, different exposure, knowledge, practices about animal handling, and self-reported Z/D/S condition. Results: Majority of study participants were females. Participants had numbers of animals, and it ranged from 1 to 70. However, the majority of them were cattle. Average experience and hours/day spent for handling animal were reported 22±15 years and 5±2 h, respectively. From all participants, about one-third perceived that handling animal could be a cause of disease. Average knowledge on the mode of transmission of Z/D/S was found 4.1%. Most common high risk and preventive practices found consumption of raw milk (72%) and handwashing (83%). The proportion of self-reported Z/D/S in the past 5 years was found to be 23% among respondents and 17% among family members. However, the proportion of existing self-reported Z/D/S or symptomatic Z/D/S was 17% among respondents and 18% among family members. Most common self-reported Z/D/S were vector-borne, animal bite, and respiratory disorders. Conclusion: The knowledge and prevalence of Z/D/S were found low as compared to other studies from India. Further awareness and screening of animal handlers can be useful to increase the reporting and prevention and control of Z/D/S among them.

scholarly journals Biological characteristics of rt181T/sW172* HBV are similar among distinct genotypes

2020 ◽  
Author(s):  
Wang Huaqiang ◽  
Ai Jun ◽  
Zhang Jiajuan ◽  
Zheng Wenkai ◽  
Wang Maorong ◽  
...  
Keyword(s):  
Hbv Dna ◽  
Biological Characteristics ◽  
Wild Type ◽  
Hbv Genotypes ◽  
Mutational Pattern ◽  
Significant Difference ◽  
Positive Rate ◽  
The Impact ◽  
Type Strains ◽  
Genotype A

Abstract Background: The influence of genotypes on disease progression and clinical outcome of HBV infection is noted. The impact of HBV genotypes on rtA181T/sW172* mutation remains unclear. Patients and Methods: Clinical characteristics of 69 patients with rtA181T/sW172* mutation and 39 patients with rtA181V mutation were analyzed in this study. Fifteen serum specimens with rtA181T/sW172* mutation from different genotypes were collected for cloning and sequence analysis. HBV markers in HepG2 cells encoding different proportions of rtA181T/sW172* mutation and wild type strains were detected in genotype A, B, C and D, respectively.Results: No statistically difference was detected between rtA181T and rtA181V group regarding mean age, sex ratio, liver functions, HBV DNA load and HBeAg positive rate, except for HBsAg level (rtA181T group = 3.07±0.54 lg IU/ml vs rtA181V group = 3.29±0.43 lg IU/ml, P=0.037). Among 69 patients with rtA181T/sW172* mutation, HBV genotypes B and C accounted for 17.0% and 83.0%, respectively. The rate of genotype B was lower in rtA181T group (10.1%) than that in rtA181V group (29.7%), whereas genotype C was higher in rtA181T group (89.9%) than that in rtA181V group (70.3%). HBV rtA181T/sW172* mutant coexisted with wild type strains, accounted for 25% to 90% in all HBV strains. The distribution proportion showed no statistical difference between genotype B and C (59.2%±24.3% vs. 61.2%±19.1% , P=0.86). In transfection experiments, the level of HBV DNA was the highest for genotype B, while HBsAg was expressed in the highest level for genotype A. HBsAg and virus particle were barely detected in the supernatants of rtA181T/sW172* HBV clones in all genotypes. As the proportion of wild type HBV plasmid increased, deficiency of rt181T/sW172* mutation was complemented in all genotypes. No significant difference of the relative expression was found among distinct genotypes (P >0.05).Conclusions: HBV rtA181T/sW172* mutational pattern may be influenced by genotypes, but biological characteristics of this mutation is similar among distinct genotypes.

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