scholarly journals Isolation and Identification of Lactic Acid Bacteria and Acetic Acid Bacteria Playing a Lead Role in the Fermentation of Cocoa in Fako Division of Cameroon

2021 ◽  
pp. 28-41
Author(s):  
Lewis D. Levai ◽  
Rauwitta O. Afoh ◽  
Yannick Tah ◽  
Ekwa Y. Monono ◽  
Lewis Enow ◽  
...  
Keyword(s):  
Acetic Acid ◽  
Lactic Acid ◽  
Lactic Acid Bacteria ◽  
Acetic Acid Bacteria ◽  
Phenotypic Characterization ◽  
Growth Media ◽  
The South ◽  
West Region ◽  
South West ◽  
South West Region

Heaps of cocoa beans and mucilage placed within plantain leaves in Ekona, Fako Division of the South West Region of Cameroon was studied to isolate and identify bacteria playing a lead role during natural fermentation process. All experiments were performed at JP Johnson Biotechnology Laboratory at IRAD Ekona, Fako Division, South West Region, Cameroon between March – October, 2020. Heaps of 10kg were placed on and covered with plantain leaves and allowed to ferment naturally. Before every sample was collected the temperature and pH were measured. Samples were inoculated into de Mann Rogosa and Sharpe (MRS) agar and Caar agar for the isolation of lactic acid bacteria (LAB) and acetic acid bacteria (AAB) respectively. Standard microbiological procedures for obtaining bacteria counts and isolation of pure cultures were performed and isolated bacteria were identified following morphological and phenotypic characterization on API50CHL for LAB and Enrichment Media for AAB. The fermentation temperature rose to 45oC after 4 days while the pH fluctuated with a peak at 4.82 after 108 hours. Maximum counts of Acetic Acid Bacteria (AAB) (5.8x108 Colony Forming Units (CFU)/ mL) and Lactic Acid Bacteria (LAB) (8.9x8x108 (CFU)/mL were obtained at 2 days and 5 days respectively. Twelve isolates each of AAB and LAB were identified on growth media. After phenotypic characterization, 03 Lactobacillus species and 02 Acetobacter species were identified. The most abundant bacteria were L. plantarum (41.77%) and Acetobacter tropicalis (24.11%) on MRS agar and Caar Agar respectively.L. plantarum and A. tropicalis have been identified as the bacteria playing a lead role in the fermentation of cocoa in Fako Division of the South West Region of Cameroon. These organisms may be used as good candidates in a consortium for use as a starter culture.

scholarly journals Lactic acid bacteria from traditionally processed corn beer and palm wine against selected food-borne pathogens isolated in south west region of Cameroon

2016 ◽  
Vol 10 (30) ◽  
pp. 1140-1147 ◽  
Author(s):  
Tatsinkou Fossi Bertrand ◽  
Anyangwe Irene ◽  
Tavea Frederic ◽  
Ebong Lucas Kome ◽  
Akenji Nkuo Theresa
Keyword(s):  
Lactic Acid ◽  
Lactic Acid Bacteria ◽  
Palm Wine ◽  
Food Borne Pathogens ◽  
West Region ◽  
South West ◽  
Food Borne ◽  
South West Region

scholarly journals Evaluation of Probiotic Potential of Lactic Acid Bacteria Isolated from Raphia Palm Wine (Raffia mambillensis)

2019 ◽  
pp. 1-14
Author(s):  
Quentin Metsatedem Tongwa ◽  
Linda Manet ◽  
Hippolyte Tene Mouafo ◽  
Bertrand Tatsinkou Fossi
Keyword(s):  
Escherichia Coli ◽  
Antimicrobial Activity ◽  
Lactic Acid ◽  
Lactic Acid Bacteria ◽  
Bile Salts ◽  
Palm Wine ◽  
Probiotic Potential ◽  
West Region ◽  
South West ◽  
South West Region

Aim: The present study was undertaken to assess the probiotic potential of lactic acid bacteria strains isolated from raphia palm wine (Raffia mambillensis O.) of the South West region of Cameroon. Study Design: The study site and sample were randomly chosen and the responses assessed were submitted to analyses of variance. Place and Duration of Study: The study was carried out in Ekona in the South West region of Cameroon, between February 2017 and October 2018. Methodology: Physicochemical analyses were performed to characterize the Raphia palm wine samples. Lactic acid bacteria were isolated from these samples using pour plate method, and the isolates were tested for their antimicrobial activity, low pH and bile salts tolerance, antibiotic sensitivity, hemolytic activity and enzymes production. The active isolates were identified phenotypically using API 50 CHL. Results: The results revealed that among the nine isolates, only three were active against Escherichia coli BL21, Escherichia coli, Salmonella typhi, Salmonella enterica and Staphylococcus aureus with inhibition diameters ranging from 10 to 25 mm. The three isolates which exhibited antimicrobial activity were able to survive at simulated stomach pH (pH 3.0) with survival rate above 80% and in the presence of bile salts at 1% with survival rates above 50%. All three isolates gave negative results for hemolytic activity and were resistant to most antibiotic treatments. These three isolates produced β-galactosidase, which is beneficial for lactose intolerance. They also produced some non-carcinogenic enzymes including leucine arylamidase, crystine arylamidase, acid phosphatase, α-galactosidase, α-glucosidase, β-glucosidase, and N-acetyl-β-glucosamidase. Identification of these three isolates with API kit 50 CHL reveals that they are Lactobacillus lactis, Lactobacillus plantarum and Lactobacillus brevis. Conclusion: Our findings revealed that raphia palm wine can be an excellent source of probiotic lactic acid bacteria.

scholarly journals Technical Efficiency Assessment of Dairy Farm in the South-west Region of Bangladesh

2020 ◽  
pp. 21-28
Author(s):  
Fariha Farjana ◽  
Afia Khatun
Keyword(s):  
Technical Efficiency ◽  
Stochastic Frontier ◽  
Percent Level ◽  
Dairy Farms ◽  
Farm Size ◽  
The South ◽  
Average Output ◽  
West Region ◽  
South West ◽  
South West Region

The paper concentrates on the measurement of the total factor productivity of dairy farms in the south-west region of Bangladesh. The study used stochastic frontier approach for analyzing the technical efficiency of the dairy farms. Here, seventy dairy farms are considered as a sample. The data reveals that the number of labor and the quantity of food are statistically significant at a 1 percent level of significance.The data also manifests that numerous farm-specific characteristics, i.e. farm size, farmer’s age, and amount of credit are statistically significant at 1 percent, 10 percent, and 10 percent respectively. The range of technical efficiency for the farms varies from 26 percent (minimum) to 95 percent (maximum) where the mean value is 68 percent for the dairy farms of the south-west region. This implies that an average output of milk production falls 32 percent short of maximum possible level. Hence, there is scope of improvement in this sector. Therefore, to improve the farm productivity government should provide proper training, and medical treatment facilities for the farms so that the animals become healthy. If it is possible to do so then the farm level production frontier will shift upward.

scholarly journals Environmental Adaptability and Rock Dust Concentrations in Lettuce Cultivars

2019 ◽  
Vol 11 (14) ◽  
pp. 306
Author(s):  
L. L. Ferreira ◽  
Carmen Rosa da Silva Curvêlo ◽  
Gileade Costa Leonel Amaral ◽  
Juliana Naves de Campos ◽  
Rodrigo Vieira da Silva ◽  
...  
Keyword(s):  
Experimental Design ◽  
Variance Analysis ◽  
The South ◽  
West Region ◽  
South West ◽  
Experimental Area ◽  
Environmental Adaptability ◽  
South West Region ◽  
Rock Dust

The purpose of this work is to analyze lettuce cultivars different responses to environmental adaptability and rock dust concentrations in agroclimatic conditions in the south-west region of Goiás state. The work was conducted in the county of Mineiros, Goiás. The experimental area soil’s was classified as quartzarenic NEOSOL. In experiment number 1, was used experimental design in random blocks in factorial 5 × 2, corresponding to five rock dust concentrations (0, 100, 200, 300 and 400 kg ha-1), in two lettuce cultivars of Crespa and Americana lettuce. In experiment number 2 was applied experimental design in randomized blocks, which were constituted by 7 lettuce cultivars (Hanson, Simpson S. Preta, Baba de Verão, Maravilha de Inverno, Grandes Lagos, Crespa Palmas, and 4 Estação). The data results were analyzed 45 days after seeds transplant. The results were submitted to variance analysis and Turkey’s regression and test at a 5% probability. The 400 kg ha-1 rock dust dose didn’t have any effects in lettuce cultivars Crespa and Americana, once that, rock dust nutrients mineralization occurs very slowly, not interfering in the lettuce first cycle. 4 Estação cultivar presented good environmental adaptability to Goiás south-west agroclimatic conditions, more specifically in Mineiros, where it is recommended lettuce cultivation in summer-fall.

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