scholarly journals Bioethanol Production from Cassava Peels Inoculated with Saccharomyces cerevisiae and Zymomonas mobilis

2021 ◽  
pp. 58-67
Author(s):  
Kehinde Tope Adegbehingbe ◽  
Foluso Faparusi ◽  
Bartholomew Saanu Adeleke
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Chemical Composition ◽  
Zymomonas Mobilis ◽  
Ph Value ◽  
Control Sample ◽  
Reducing Sugar ◽  
Bioethanol Production ◽  
Transportation Fuel ◽  
Cassava Peels ◽  
Cassava Peel

Aim: This study was designed to determine the bioethanol production from fermented cassava peel using Saccharomyces cerevisiae and Zymomonas mobilis. Methodology: Cassava peels were collected from cassava processing sites, washed, surface sterilized, dried, milled into flour, pretreated, and fermented. Saccharomyces cerevisiae and Z. mobilis suspensions were aseptically inoculated into the fermenting medium and allowed to stand for seven days. The pH, total reducing sugar, chemical composition, and bioethanol composition of the fermenting substrates were determined. Results: A pH decrease from 5.2 to 4.1 was recorded in the sample fermented with S. cerevisiae while the least pH value of 3.8 was obtained from the sample fermented with Z. mobilis, respectively. The total reducing sugar (glucose) of fermented samples decreased from 3.4% to 1.5% (Z. mobilis) and 3.4% to 1.88% (S. cerevisiae) compared with the control sample. The chemical composition showed high protein and fat contents in the fermented samples. High percentage yield of 30% with ethanol volume of 45 mL was recovered from cassava peel inoculated with S. cerevisiae while flash point, i.e. the lowest temperature at which fuel produces enough vapor to cause ignition leading to flame generation of 24oC, was recorded for both fermented samples inoculated with S. cerevisiae and Z. mobilis. Conclusion: The ability of the bacterium and the yeast isolates exhibiting high potential for bioethanol production could be promising in various industrial processes as an alternative to fossil transportation fuel.

Bioethanol Production from Pineapple Peel Juice Using Saccharomyces cerevisiae

2014 ◽  
Vol 875-877 ◽  
pp. 242-245
Author(s):  
Jutarut Pornpunyapat ◽  
Wilaiwan Chotigeat ◽  
Pakamas Chetpattananondh
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Ethanol Production ◽  
Ethanol Concentration ◽  
Renewable Resource ◽  
Reducing Sugar ◽  
Total Sugar ◽  
Bioethanol Production ◽  
Pineapple Peel ◽  
Initial Ph ◽  
Production Characteristics

Bioethanol is widely used as renewable resource due to its safe to produce and environmentally friendly. However, knowledge on ethanol production from pineapple peel juice (Pattawia spp) is far from sufficient. In this work, pineapple peel juice (initial pH at 5) was fermented at various yeast contents (1, 3 and 5% by wt.) and fermentation times (3, 5 and 7 days) in order to investigate ethanol production characteristics. Yeast, Sacchromyces cerevisiae was grown on pineapple peel juice. The squeezed juice contained 11% of total sugar and 5% of reducing sugar. The results indicated that the optimum ethanol production was yeast contents of 5% by wt. and fermentation times of 5 days which gave the ethanol production of 9.08g/l. The ethanol at a higher yeast content also had a higher ethanol concentration.

scholarly journals DEGRADASI KOMPONEN SELULOSA, HEMISELULOSA, DAN PATI TEPUNG KULIT UBI KAYU MENJADI GULA REDUKSI OLEH Aspergillus niger, Trichoderma viride, DAN Acremonium sp. IMI 383068

2019 ◽  
Vol 13 (01) ◽  
pp. 34
Author(s):  
Jay Jayus ◽  
Ahmad Nafi' ◽  
Anis Shabrina Hanifa
Keyword(s):  
Trichoderma Viride ◽  
Hydrolytic Enzyme ◽  
Reducing Sugar ◽  
Mixed Cultures ◽  
Single Strain ◽  
Hydrolysis Process ◽  
Hydrolysis Of ◽  
Cassava Peels ◽  
Cassava Peel ◽  
Single Enzyme

As the solid waste produced from cassava processing industry such as tapioca factory or its derivatives, the cassava peel is potential to be use as a source of reducing sugar through hydrolysis process, since the peels contains a high amount of starch and lignocellulose components. The more environmentally friendly enzymatic hydrolysis using several microorganisms will be introduced in this study as an alternative to avoid the unsafe acid hydrolysis. However, the hydrolysis process using a single microorganism is not efficient since the hydrolytic enzyme produced is limited to a single enzyme, while the component in the cassava peels to be hydrolyzed is diverse which include cellulose, lignin, hemicellulose and starch. Therefore, it is necessary to optimize the hydrolysis process by combining several microorganisms (A. niger, T. viride and Acremonium sp. IMI 383068) which produced different specificity of hydrolytic enzyme depending on the substrate available in the cassava peels. The aims of this research were to determine the effect of single and mixed culture on the amount of reducing sugar released during the simultaneous cultivation. The result showed that the use of simultaneous mixed cultures during hydrolysis process was able to produce higher reducing sugar compare to that of single culture. The hydrolysis of cassava flour using a single strain of A. niger, T. viride and Acremonium sp. IMI 383068 respectively produced 4.86 g/L, 4.02 g/L, and 1.68 g/L of reducing sugar, while the hydrolysis of it using simultaneous mixed cultures of A. niger, T. viride, and Acremonium sp. IMI 383068 produced 7.23 g/L of reducing sugar. Keywords: cassava peels, hydrolysis, reducing sugar

scholarly journals Design Process of Hydrolysis and Fermentation Bioethanol Production from Seaweed Eucheuma cottonii to Renewable Energy Sovereignity

2016 ◽  
Vol 3 (3) ◽  
pp. 107
Author(s):  
Wagiman . ◽  
Makhmudun Ainuri ◽  
Rinda Gusvita ◽  
Jumeri .
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Process Design ◽  
Sugar Content ◽  
Reducing Sugar ◽  
Bioethanol Production ◽  
Fermentation Efficiency ◽  
Hydrolysis Process ◽  
High Reduction ◽  
Eucheuma Cottonii ◽  
Fermentation Substrate

<p>The aim of this research was study of E. cottonii to produce bioethanol fermentation substrate with a high reduction sugar content and low Hidroxymethilfurfural (HMF). Fermentation done by instant yeast and Saccharomyces cerevisiae culture of FNCC 3012.The best treatment was obtained in the combination of 2% of H2SO4 by time reaction of 120 minutes in 80°C produced 15.61 g/l reducing sugar and 5.03 g/l HMF. In fermented process, the hydrolysate with instant yeast starter delivered much more efficiency in 3.63 ml CO2 volume, 87.53% in fermentation efficiency, and 1.96 g/l reducing sugar on fifth day of fermentation. <br /><strong>Keywords</strong>: bioethanol, Eucheuma cottonii, fermentation, hydrolysis, process design</p>

scholarly journals Comparative study of bioethanol production from sugarcane molasses by using Zymomonas mobilis and Saccharomyces cerevisiae

2015 ◽  
Vol 14 (31) ◽  
pp. 2455-2462 ◽  
Author(s):  
H Khoja Asif ◽  
Ali Ehsan ◽  
Zafar Kashaf ◽  
A Ansari Abeera ◽  
Nawar Azra ◽  
...  
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Comparative Study ◽  
Zymomonas Mobilis ◽  
Sugarcane Molasses ◽  
Bioethanol Production

scholarly journals Bioethanol production by Saccharomyces cerevisiae, Pichia stipitis and Zymomonas mobilis from delignified coconut fibre mature and lignin extraction according to biorefinery concept

2016 ◽  
Vol 94 ◽  
pp. 353-365 ◽  
Author(s):  
Fabiano Avelino Gonçalves ◽  
Héctor A. Ruiz ◽  
Everaldo Silvino dos Santos ◽  
José A. Teixeira ◽  
Gorete Ribeiro de Macedo
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Zymomonas Mobilis ◽  
Pichia Stipitis ◽  
Bioethanol Production ◽  
Lignin Extraction ◽  
Coconut Fibre

scholarly journals Sustainable Production of Bioethanol by Zymomonas mobilis and Saccharomyces cerevisiae using Rice Husk and Groundnut Shell as Substrates

2021 ◽  
pp. 15-26
Author(s):  
A. J. Na’Allah ◽  
M. Y. Iliyasu ◽  
U. S. Haruna ◽  
A. Ahmad ◽  
S. O. Oguche ◽  
...  
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Rice Husk ◽  
Zymomonas Mobilis ◽  
Renewable Energy Sources ◽  
Reducing Sugar ◽  
Crude Lipid ◽  
Local Strains ◽  
Standard Curve ◽  
Fermented Beverage ◽  
Groundnut Shell

Background of Study: Plant waste such as rice husk and groundnut shell are generated in large amounts, these waste presents a tremendous pollution to the environment. Worldwide, these wastes are often simply dumped into landfills and oceans or used as animal feeds. The recovery of food processing wastes as renewable energy sources represents a sustainable option for the substitution of fossil energy in order to minimize environmental damages and to meet energy demands of the growing population. Aim: To produce bioethanol from rice husk and groundnut shell using local strains of Zymomonas mobilis and Saccharomyces cerevisiae. Place and Duration of Study: Conducted at the Microbiology Laboratory of Abubakar Tafawa Balewa University Bauchi, Bauchi state, Nigeria, between April to June, 2021. Methods: Groundnut shell and Rice husk were collected from local milling center. The wastes were powdered, sieved and used as carbon source. Proximate composition of the subsrate was done and the total carbohydrate was determined by difference. The sum of the percentage moisture, ash, crude lipid, crude protein and crude fibre was subtracted from 100. Zymomonas mobilis and Saccharomyces cerevisiae were isolated from rotten sweet oranges and locally fermented beverage (‘kunun-zaki’) respectively by growing them on Malt Yeast Peptone Glucose Agar (MYPGA) after which they were further screened for their ability to tolerate ethanol and they serve as organisms for fermentation. The enzyme α- amylase was used for hydrolysis. The fermented substrates were distilled at 78oC and the distillate was collected as bioethanol in a conical flask. UV-VIS spectrophotometer was used to determine the absorbance of each concentration (0, 0.2, 0.4, 0.6 and 0.8cm3) of reducing sugar content of the hydrolysates and the bioethanol produced by developing a standard curve at a wavelength of 491nm and 588nm respectively. The concentration of reducing sugar and bioethanol was determined using a reference line from the Standard curve. Results: Proximate analysis done shows that rice husk have 70.09% carbohydrates while groundnut shell has 65.09% carbohydrates. Groundnut shell yielded the highest reducing sugar of 5.096%. Rice husk yielded the lowest quantity of reducing sugar with a total yield of 2.962%. Maximum concentration of bioethanol of 0.971% was produced from the combination of Saccharomyces cerevisiae and Zymomonas mobilis from groundnut shell. The lowest concentration of 0.121% of bioethanol was produced when Saccharomyces cerevisiae was used on rice husk hydrolysates. The synergistic relationship of Saccharomyces cerevisiae and Zymomonas mobilis yielded the maximum bioethanol when compared with the yield obtained when the organisms were used singly. Zymomonas mobilis produced highest bioethanol content when the organisms are used single. Conclusion: This study demonstrates the potentiality of local strains of Saccharomyces cerevisiae and Zymomonas mobilis isolated from rotten sweet orange and locally fermented beverage (‘kunun-zaki’) to produce bioethanol by fermenting the rice husk and groundnut shell hydrolysates.

scholarly journals Optimization of bioethanol production from cassava peels

2021 ◽  
Vol 24 (12) ◽  
pp. 2077-2083
Author(s):  
S.O. Osemwengie ◽  
E.I. Osagie ◽  
B. Onwukwe
Keyword(s):  
Incubation Time ◽  
Ethanol Yield ◽  
Bioethanol Production ◽  
Enzyme Loading ◽  
Hydrolysis Time ◽  
Total Carbohydrate ◽  
Total Carbohydrate Content ◽  
Cassava Peels ◽  
Cassava Peel ◽  
Simultaneous Saccharification

The bioethanol production from waste is acquiring attraction as a strategy for increasing energy security. This study aims to optimize the production of ethanol from cassava peel using Box Bhenken experimental design. The total carbohydrate content of about 90% in cassava peel was subjected to enzymatic hydrolysis using Alpha-amylase followed by Simultaneous Saccharification and Fermentation (SSF) by Saccharomyces cerevisiae for bioethanol production. The production of bioethanol from cassava peels was investigated for 1-4 hours (hydrolysis time), 0.5–1.5mg/L (enzyme loading), and 1-5 days (incubation time). A statistical model was developed and validated to predict the yield of bioethanol after fermentation, and the Response Surface Methodology (RSM) was used to optimize the conditions. The results revealed that the maximum ethanol yield of 1.911% was obtained at the optimum hydrolysis time, enzyme loading, and incubation time (i.e. 2.5 hours, 1 mg/L, and 3 days respectively).

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