scholarly journals Haemostatic Alterations after Sildenafil and Tramadol Administration in Rats

2020 ◽  
pp. 153-158
Author(s):  
Evelyn Mgbeoma Eze ◽  
Ransom Baribefii Jacob
Keyword(s):  
Coagulation Factors ◽  
Coagulation System ◽  
Albino Rats ◽  
Platelet Distribution Width ◽  
Platelet Volume ◽  
Extrinsic Pathway ◽  
Distribution Width ◽  
Significant Difference ◽  
Group A ◽  
The Mean

Haemostatic parameters constitute measurable indices in the haemostatic system used to assess the functionality of the coagulation system of an individual to establish a state of health or disorder. This study evaluated haemostatic parameter such as platelets count, mean platelet volume (MPV), platelets distribution width (PDW), prothrombin time (PT) and activated partial thromboplastin time (APTT) in 22 Male Albino Rats grouped and orally treated daily for three weeks with Sildenafil (4 mg/200 g.bwt), Tramadol(6 mg/200 g.bwt) and Sildenafil/Tramadol combination (4+6 mg/220 g.bwt). Rats were sacrificed by cardiac puncture and 5 mls of blood collected for the analysis of the parameters using Sysmex haematology analyser and Agape Diagnostic reagents kits. Results obtained shows a statistically significant increase in platelet count, PT and APTT compared with control across the various groups (p<0.05). A statistically significant decrease was observed in MPV, PDW in Sildenafil+tramadol group, significant decrease in platelets distribution width for Tramadol group when compared with control (p<0.05). No significant difference was observed in the mean platelets volume and platelet distribution width in Sildenafil group. A comparison of Sildenafil+tramadol and Sildenafil groups shows no statistically significant difference in all the parameters analysed. There was also no significant difference in the mean platelets count, PDW, PT and APTT when Sildenafil+tramadol and Tramadol groups were compared (p<0.05). However, a statistically significant increase was seen in platelets count when Sildenafil+tramadol and tramadol were compared (p<0.05). Sildenafil and tramadol causes significant increase in platelets count, prolonged PT and APTT following single/combined daily administration in rats. Further research on these parameters, assessment of liver function, and measurement of intrinsic and extrinsic pathway coagulation factors in human taking this medication is recommended.

Effects of Thioxanthene Containing Anti-Psychotic and Anti-Platelet Drug Combination on Mean Platelet Volume and Platelet Distribution Width in Rats

2019 ◽  
pp. 1-7
Author(s):  
Muhammad Irfan Bashir ◽  
Uzma Saleem ◽  
Fareeha Anwer ◽  
Bashir Ahmad
Keyword(s):  
Mean Platelet Volume ◽  
Individual Therapy ◽  
Antiplatelet Drug ◽  
Combination Group ◽  
Albino Rats ◽  
Platelet Distribution Width ◽  
Anti Psychotic ◽  
Platelet Volume ◽  
Distribution Width ◽  
The Individual

Aim: To evaluate the combined and individual effects of thioxanthene containing antipsychotic and anti-platlet drug on Mean Platelet Volume (MPV) and Platelet Distribution Width (PDW) in rats. Methods: This investigational study comprised of 100 albino rats of both sexual orientation, they were of 300 g to 350 g. We got ten groups, in which each group consisted of 10 rats (n=10).Ozagrel was used as anti-platelet and Zuclopenthixol was used as thioxanthene containing antipsychotic. Rats were treated with defined doses of Ozagrel and thioxanthene containing anti-psychotic (Zuclopenthixol) alone and in joined for three weeks (21 days). Blood test at 0, seventh, fourteenth and last day of study were taken. Mean Platelet Volume and Platelet Distribution Width were estimated from blood tests by using standard research center procedure. Results were accumulated and abridged by applying statistics. Correlation was framed between all days incentive to zero day values. Results: Anti-psychotic drug and antiplatelet drug both showed decrease in MPV with both doses highly significantly (p < 0.001) decrease associated with maximum doses alone and with combination group. In case of PDW all individual and minimum combination group showed increase in PDW values but significantly (p < 0.001) increase in maximum combination groups. Conclusion: Combination of both drugs can cause more decrease in MPV as compare to the individual and in case of PDW combination with maximum doses may cause decrease in values by opposing the results individual therapy. It indicated any Drug-Drug interaction between these two drugs regarding Mean platelet volume and Platelet distribution width.

scholarly journals Association of platelet count and platelet indices with cranial meningioma

2020 ◽  
pp. 123-126
Author(s):  
Yasar Karatas ◽  
Fatih Keskin ◽  
Mehmet Fatih Erdi ◽  
Bulent Kaya ◽  
Densel Arac ◽  
...  
Keyword(s):  
Platelet Count ◽  
Mean Platelet Volume ◽  
Small Sample ◽  
Control Group ◽  
Platelet Distribution Width ◽  
Platelet Volume ◽  
Distribution Width ◽  
Healthy Group ◽  
Platelet Indices ◽  
Significant Difference

Introduction and Objectives: In this study, we aimed to investigate whether platelet count (PLT) and platelet indices included mean platelet volume (MPV), platecrit (PCT), platelet distribution width  (PDW) values can be used as diagnostic markers in cranial meningiomas. Materials and Methods: The study included results of 29 patient and 47 healthy contributors. Based on pathologies, the patients were divided into two groups. The first group included meningioma patients and the second one included healthy individuals. Healthy contributors named control group. Platelet count and platelet indices were determined using Sysmex XN 550 haematology analyzer. The preoperative platelet count (PLT) and platelet indices included mean platelet volume (MPV), platecrit (PCT), platelet distribution width  (PDW) values were recorded from the routine laboratory tests. Results: There was no statistically significant difference in PLT between the meningioma and healthy groups (p = 0.217). There was a statistically significant difference in PCT between the meningioma group and the healthy group (p = 0.002). There was a statistically significant difference in PDW between meningioma group and healthy group (p = 0.001). In terms of MPV, there was a statistically significant difference between meningioma group and the healthy group (p = 0.001) Conclusion: Platelet count and indices are easily available in the routine blood tests. Despite the retrospective design and small sample size, our findings suggest that altered MPV, PDW and PCT levels might serve as potential biomarkers for the diagnosis of meningiomas.

scholarly journals Evaluation of the Mean Platelet Volume and Platelet Distribution width in Children with Familial Mediterranean Fever

2020 ◽  
Vol 18 (2) ◽  
pp. 208-219
Author(s):  
Fatma Duksal ◽  
Ahmet Sami Güven ◽  
Mesut Arslan ◽  
Melih Timucin Dogan ◽  
Utku Aygüneş
Keyword(s):  
Familial Mediterranean Fever ◽  
Mean Platelet Volume ◽  
Platelet Distribution Width ◽  
Platelet Volume ◽  
Distribution Width ◽  
Mediterranean Fever ◽  
The Mean

scholarly journals Study of Platelet Indices and Their Interpretation in Thrombocytopenia in a Tertiary Care Hospital

2021 ◽  
Vol 10 (7) ◽  
pp. 435-439
Author(s):  
Vani Mittal ◽  
Munesh Munesh ◽  
Irbinder Kour Bali ◽  
Sunil Arora ◽  
Jyoti Singh ◽  
...  
Keyword(s):  
Platelet Count ◽  
Mean Platelet Volume ◽  
Large Cell ◽  
Care Hospital ◽  
Platelet Distribution Width ◽  
Platelet Volume ◽  
Distribution Width ◽  
Cell Ratio ◽  
Platelet Indices ◽  
Significant Difference

BACKGROUND Thrombocytopenia may either be due to increased destruction or impaired production of platelets. Platelet count alone is not enough to determine the mechanism of low platelets. Platelet indices like mean platelet volume (MPV), platelet distribution width (PDW), platelet large cell ratio (P-LCR) and platelet crit (PCT) can help determine the cause and we aimed at finding their role and function in cases of thrombocytopenia. METHODS An observational cross-sectional study of 155 patients with thrombocytopenia and 71 controls was done for a period of six months in SGT Hospital, Gurugram, to determine the mechanism behind the low platelet count with the help of these indices. RESULTS The mean values of the platelet indices (PDW, P-LCR and PCT) were found to be higher in accelerated destruction group (P < 0.05) in comparison to hypoproductive group, whereas, mean MPV values were higher in the former, but was not statistically significant. On comparison with the controls, both the groups of thrombocytopenia showed a statistically significant difference with P < 0.005 in all the four indices. Mean PCT values showed a highly significant difference between the two groups as well as with controls (P < 0.001) and also the relationship of PCT with severity of thrombocytopenia showed a direct relationship which was also significant (P < 0.001). CONCLUSIONS In distinguishing between the cause of thrombocytopenia i.e., hypoproductive or hyper destruction, platelet parameters play an important role. These platelet indices are easily available with the help of automated haematology analysers and can reduce the need for costly and invasive tests for evaluation of thrombocytopenia. KEY WORDS Mean Platelet Volume, Platelet crit, Platelet Distribution Width, Platelet Large Cell Ratio, Thrombocytopenia

scholarly journals Platelet distribution width, mean platelet volume and haematological parameters in patients with uncomplicated plasmodium falciparum and P. vivax malaria

F1000Research ◽  
2017 ◽  
Vol 6 ◽  
pp. 865 ◽  
Author(s):  
Elrazi A. Ali ◽  
Tajeldin M. Abdalla ◽  
Ishag Adam
Keyword(s):  
Plasmodium Falciparum ◽  
Mean Platelet Volume ◽  
Malaria Infection ◽  
Haematological Parameters ◽  
Published Data ◽  
Platelet Distribution Width ◽  
Platelet Volume ◽  
Distribution Width ◽  
Significant Difference ◽  
Haematological Profile

Background: The association between the haematological profile (including abnormal platelets) and malaria is not completely understood. There are few published data on haematological profiles of malaria patients in areas with unstable malaria transmission. The current study was conducted to investigate if the haematological parameters, including platelet indices, were reliable predictors for microscopically-diagnosed malaria infection. Methods: A case-control study with a total of 324 participants (162 in each arm) was conducted at the out-patient clinic of New Halfa hospital during the rainy and post rainy season (August 2014 through to January 2015). The cases were patients with uncomplicated Plasmodium falciparum (107; 66.9%) and P. vivax malaria (55, 34.0%) infections. The controls were aparasitemic individuals. The haematological parameters were investigated using an automated hemo-analyser. Results: There was no significant difference in the mean (±SD) age between the study groups; however, compared to the controls, patients with uncomplicated malaria had significantly lower haemoglobin, leucocyte and platelet counts, and significantly higher red cell distribution width (RDW), platelet distribution width (PDW) and mean platelet volume (MPV). Conclusions: The study revealed that among the haematological indices, PDW and MPV were the main predictors for uncomplicated P. falciparum and P. vivax malaria infection. Abbreviations: OR: odds ratio.

Flow Cytometry With Inflorescence-An Accessible Method in the Study of Qualitative and Quantitative Modifications of Blood Platelets in Immune Thrombocytopenic Purpura

2018 ◽  
Vol 69 (9) ◽  
pp. 2476-2478
Author(s):  
Oana Viola Badulescu ◽  
Magda Badescu ◽  
Manuela Ciocoiu ◽  
Madalina Mocanu
Keyword(s):  
Flow Cytometry ◽  
Immune Thrombocytopenic Purpura ◽  
Mean Platelet Volume ◽  
Blood Platelets ◽  
Platelet Distribution Width ◽  
Platelet Volume ◽  
Thrombocytopenic Purpura ◽  
Distribution Width ◽  
Accessible Method ◽  
The Mean

The immune thrombocytopenic purpura (ITP) is a polymorphic and hematologic pathology, in terms of both clinical manifestations and etiopathogenic mechanisms, which bring on this disease. Establishing the type of thrombocytopenia, which can either be primary or secondary, peripheral or central, is essential for a further proper therapeutic conduct. The current means of diagnosis include a large variety of hematologic, immunologic and biochemical explorations, from the traditional ones to the latest methods, either genetical or molecular. The purpose of this paper is to emphasize the importance of a classical method of diagnosis, used in the primary triage of patients suffering from thrombocytopenia, highly valuable in the diagnosis process: the analysis of the mean platelet volume (MPV) and of the platelet distribution width (PDW), parameters revealed by the CBC (complete blood count). Evaluating the size of the platelets is a useful tool in the differential diagnosis between immune thrombocytopenic purpura and hereditary macrothrombocytopenia. The platelet distribution width and the mean platelet volume are read simultaneously, in order to differentiate the immune peripheral thrombocytopenia from the central one. The classical, viable and accessible method facilitates the measurement of MPV and PDW with the automatic analyzer, which functions according to the principle of flow cytometry with inflorescence, by using a LASER semiconductor and hydrodynamic focusing.

scholarly journals Platelet indices evaluation in patients with dengue fever

2018 ◽  
Vol 6 (6) ◽  
pp. 2054 ◽  
Author(s):  
Payal Mukker ◽  
Smitha Kiran
Keyword(s):  
Platelet Count ◽  
Dengue Fever ◽  
Mean Platelet Volume ◽  
Dengue Infection ◽  
P Value ◽  
Platelet Distribution Width ◽  
Platelet Volume ◽  
Distribution Width ◽  
Platelet Indices ◽  
Significant Difference

Background: Platelet indices (PIs)-Platecrit, mean platelet volume (MPV) and platelet distribution width (PDW)-are a group of platelet parameters obtained as a part of complete blood count using automated hematology analyzers. Evidence suggests that PIs may have diagnostic and prognostic value in febrile thrombocytopenia. This study aims to understand the profile of PIs in dengue fever. Aims and objectives was to study the platelet indices in patients with dengue fever.Methods: The present study is a retrospective observational study conducted in a tertiary hospital in Kerala. Platelet count, mean platelet volume (MPV), platecrit and Platelet distribution width (PDW) along with routine blood parameters hemoglobin(Hb), hematocrit, WBC, Platelet count, serum bilirubin, liver enzymes (AST, ALT) of 123 patients were collected. These 123 patients were grouped into three according to the platelet count (<20000,20000-100000, >100000). All the test results are available in hospital database. This was accessed using inpatient numbers obtained from medical records department of our institution. All analysis was done using free to use software R and values were rounded off to the nearest decimal point. Non normally distributed parameters were expressed as median (IQR). Parameters which did not follow normal distribution were analyzed with Kruskall Wallis test and the values are expressed as mean (SD) and a p value <0.05 was considered statistically significant.Results: Platelet indices PDW (57±13.8 vs. 55.4±6.9, p value 0.001) and MPV (9.2±0.09 vs. 13.8±1.3fL, p value <0.001) values were significantly altered in dengue fever with platelet counts below 20,000 compared to platelet count more than one lakh group. Similarly, the Platelet index (MPVxPDW\PLCxPCT), MPV\PLC, MPV\Platecrit, PDW\PLC and PDW\Platecrit ratio showed statistically significant difference between the different platelet groups.Conclusions: Platelet indices are useful parameters in dengue infection. Other than platelet count, PDW, MPV, platecrit are useful to monitor dengue fever.

scholarly journals The relation between mean platelet volume and platelet distribution width to COVID-19 severity

2021 ◽  
Vol 8 (8) ◽  
pp. 1026
Author(s):  
I. Kadek Aditya Nugraha ◽  
Kadek Surya Atmaja ◽  
Ketut Suryana ◽  
Wayan Wahyu Semara Putra
Keyword(s):  
Mean Platelet Volume ◽  
Wide Spectrum ◽  
Platelet Distribution Width ◽  
Platelet Volume ◽  
Distribution Width ◽  
Significant Difference ◽  
Auc Value ◽  
Relationship Of ◽  
D Dimer

Background: Wide spectrum of clinical manifestation and severity of COVID-19 led to further studies to find a simple biomarker used to predict the severity of COVID-19. We investigated the role of mean platelet volume (MPV) and platelet distribution width (PDW), widely available parameters, as predictor of COVID-19 severity.Methods: We conducted a single center analytic observational study to evaluate the relationship between MPV and PDW values and COVID-19 severities. A total of 123 subjects of COVID-19 within October to December 2020 included in the analysis.Results: Analysis showed a statistically significant difference in MPV, PDW, and D-dimer between COVID-19 severities (p<0.001, p=0.002, and p<0.001). Correlation coefficient between MPV, PDW, and D-dimer with COVID-19 severity were 0.28 (p=0.002); 0.22 (p<0.001), and 0.81 (p<0.001), but there’s no correlation between MPV (p=0.176) and PDW (p=0.383) with D-dimer. The AUC value of the ROC curve of MPV, PDW and D-dimer in predicting severity was 79% (p=0.001, 95% CI: 0.696-0.885), 72.5% (p<0.001, 95% CI: 0.598-0.852), and 97% (p<0.001, 95% CI: 0.937-1.00).Conclusions: This study found a relationship between MPV and PDW values on the severity of COVID-19. There’s no relationship of MPV and PDW to D-dimer concentration.

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