scholarly journals Comparative Evaluation of the Efficacy of Newer Irrigating Solutions in the Removal of Smear Layer Using SEM

2021 ◽  
pp. 76-83
Author(s):  
Srivalli Podili ◽  
Parvataneni Krishna Prasad ◽  
T. B. V. G. Raju ◽  
Mahendra Varma Nadimpalli ◽  
Gowtam Dev Dondapati ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Phytic Acid ◽  
Root Canal ◽  
Comparative Evaluation ◽  
Smear Layer ◽  
Control Group ◽  
Layer Removal ◽  
Significant Difference ◽  
Conventional Irrigation ◽  
Scanning Electron

Aim: To compare the effect of EDTA, Phytic acid and Peracitic acid on smear layer removal by effective conventional irrigation. Materials and Methodology: A total of 50 extracted mandibular premolars were selected. The canals were instrumented by rotary system up to F2 ProTaper and irrigated with 3% NaOCL simultaneously, teeth were divided into 4 groups according to the final irrigants: 17% EDTA; 2.25% PERACITIC ACID; 1% Phytic Acid; and saline. The canals were irrigated with 25 guage side vented needles for 5 minutes. Specimens were examined under scanning electron microscopy (SEM). Smear layer removal was evaluated at coronal, middle and apical thirds of the root canal. The data is analyzed using the Kruskal-Wallis and Mann-Whitney U tests. Results: In this study, the control group that is normal saline (4.05 ± 1.06) showed very less amount of smear layer removal. The highest amount of smear layer removal was seen in the group with phytic acid followed by peracitic acid and sodium hypochlorite groups. The apical third showed significantly more smear layer than the coronal and middle thirds (p<0.05). No significant difference was found between the coronal and middle thirds in all experimental groups (p>0.05). Conclusion: 1% phytic acid was the most effective in removing the smear layer at each level of the root canal followed by 2.5% peracitic and 17% EDTA.

scholarly journals The Effectiveness of Three Different Plant Extracts Used as Irrigant in Removal of Smear Layer: A Scanning Electron Microscope Study

2015 ◽  
Vol 9 (1) ◽  
pp. 16-22 ◽  
Author(s):  
A Gupta ◽  
V Goyal ◽  
J Duhan ◽  
S Hans ◽  
P Sangwan
Keyword(s):  
Plant Extracts ◽  
Root Canal ◽  
Smear Layer ◽  
Ocimum Sanctum ◽  
Control Group ◽  
Syzygium Aromaticum ◽  
Distilled Water ◽  
Scanning Electron Microscope Study ◽  
Layer Removal ◽  
Scanning Electron

ABSTRACT Aim In the present study, the role of three plant extracts as irrigant in root canal cleaning after instrumentation was evaluated. The effect of Syzygium aromaticum (S. Aromaticum), Ocimum sanctum (O. Sanctum) and Cinnamomum zeylanicum (C. zeylanicum) plant extracts was evaluated in smear layer removal. Methods The study was divided into different groups having 5 teeth each using various irrigating agents to evaluate smear layer removal. Group A: O. Sanctum extract; group A1: O. Sanctum extract with EDTA, group B: S. Aromaticum extract; group B1 S. Aromaticum extract with EDTA, group C: C. zeylanicum extract; group C1 C. zeylanicum extract with EDTA and two control group of 5 teeth each in group D: 3% NaOCl; group D1 3% NaOCl with EDTA (as positive control) and group E: Distilled water (as negative control); group E1 3% Distilled water with EDTA. Each tooth was split longitudinally and prepared for examination by scanning electron microscopy. Results The herbal extracts were effective in cleaning root canal walls when combine with EDTA with maximum activity of S. Aromaticum extract with EDTA group. Conclusion Under the condition of present study the three herbal plant extracts were ineffective in removal of smear layer when used alone.

scholarly journals A Comparative Evaluation of Cleaning Efficacy (Debris and Smear Layer Removal) of Hand and Two NiTi Rotary Instrumentation Systems (K3 and ProTaper): A SEM Study

2013 ◽  
Vol 14 (6) ◽  
pp. 1028-1035
Author(s):  
Sangeetha Vallikanthan ◽  
K Balakoti Reddy ◽  
Shreemoy Dash ◽  
Sowmya Kallepalli ◽  
N Chakrapani ◽  
...  
Keyword(s):  
Root Canal ◽  
Comparative Evaluation ◽  
Group Iv ◽  
Smear Layer ◽  
Layer Removal ◽  
Group I ◽  
Rotary Instruments ◽  
Significant Difference ◽  
Sem Study ◽  
Cleaning Efficacy

ABSTRACT Objectives The present study was conducted to compare the cleaning efficacy (debris and smear layer removal) of hand and two NiTi rotary instrumentation systems (K3 and ProTaper). Materials and methods Sixty single rooted human maxillary anterior teeth decoronated at the cementoenamel junction were used. All the specimens were divided into four groups of 15 teeth each, group I—ProTaper rotary instrumentation done, group II—K3 rotary instrumentation done, group III—Stainless steel K-file instrumentation done, group IV—root canal irrigation without instrumentation. Root canal preparation was done in a crown down manner and 3% sodium hypochlorite was used as irrigant after each file followed by final rinse with 5 ml of 17% EDTA solution, then specimens were scanning electron microscopic (SEM) examination. Results Statistical analysis was done using one-way ANOVA followed by post hoc Tukey's HSD test. Group I showed highly statistical significant difference compared to other groups. There was no statistically significant difference considering smear layer at any levels among the groups with no smear layer formation in group IV. Conclusion ProTaper rotary instrumentation showed the maximum cleaning efficacy followed by K3 rotary instrumentation in the coronal, middle and apical thirds of the root canal. Clinical significance ProTaper rotary instruments are more efficient than hand and K3 rotary instruments during root canal treatment. How to cite this article Reddy KB, Dash S, Kallepalli S, Vallikanthan S, Chakrapani N, Kalepu V. A Comparative Evaluation of Cleaning Efficacy (Debris and Smear Layer Removal) of Hand and Two NiTi Rotary Instrumentation Systems (K3 and ProTaper): A SEM Study. J Contemp Dent Pract 2013;14(6):1028-1035.

scholarly journals Residual smear layer after root canal instrumentation by using Niti, M-Wire and CM-Wire instruments: A scanning electron microscopy analysis

2018 ◽  
Vol 12 (03) ◽  
pp. 403-409 ◽  
Author(s):  
Ricardo Machado ◽  
Daniel Comparin ◽  
Eduardo Donato Eing Engelke Back ◽  
Lucas da Fonseca Roberti Garcia ◽  
Luiz Rômulo Alberton
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Root Canal ◽  
Smear Layer ◽  
Significant Difference ◽  
Electron Microscopy Analysis ◽  
Microscopy Analysis ◽  
Root Canal Instrumentation ◽  
Reciprocating Systems ◽  
Scanning Electron

ABSTRACT Objective: The purpose of this study was to compare the amount of residual smear layer after root canal instrumentation by using Ni-Ti, M-Wire, and CM-Wire instruments. Materials and Methods: Seventy-two mandibular incisors were randomly divided into six groups according to the system used: WaveOne (WO), Reciproc (RP), Unicone (UC), ProTaper Next (PN), Mtwo (MT), and HyFlex (HF). Afterward, the specimens were cleaved in the mesiodistal and buccolingual direction for analysis by scanning electron microscopy. Results: Considering both directions and root canal thirds, there was no difference between HF, MT, and PN. RP, UC, and WO presented a significant difference between the directions, and the cervical third showed a significantly smaller quantity of residual smear layer compared with the apical third. When the systems were compared among them, there was a significant difference only between RP and WO. Conclusions: Residual smear layer observed after instrumentation with the different systems was similar, except for quantities between the reciprocating systems.

scholarly journals Effect of Intermediate Flush Using Different Devices to Prevent Chemical Smear Layer Formation

2017 ◽  
Vol 28 (4) ◽  
pp. 447-452
Author(s):  
Camilla Corrêa Silva ◽  
Vivian Maria Durange Ferreira ◽  
Gustavo De-Deus ◽  
Daniel Rodrigo Herrera ◽  
Maíra do Prado ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Root Canal ◽  
Smear Layer ◽  
Distilled Water ◽  
Layer Formation ◽  
Rank Tests ◽  
Signed Rank ◽  
Chlorhexidine Solution ◽  
Conventional Irrigation ◽  
Scanning Electron

Abstract This study compared the effect of intermediate flush with distilled water delivered by conventional irrigation, EndoVac microcannula or Self-Adjusting File (SAF) system in the prevention of chemical smear layer (CSL) formation. Thirty human premolars were used. Canals were prepared with Reciproc system and 5.25% NaOCl. After chemomechanical preparation, samples were divided in 3 groups (n=10) according to the intermediate irrigation protocol with distilled water using: conventional irrigation, EndoVac microcannula or SAF. A final flush with 2% chlorhexidine solution was used and scanning electron microscopy was performed to assess protocol effectiveness. Two calibrated evaluators attributed scores according the presence or absence of CSL on the surface of the root canal walls at the coronal, middle and apical thirds, as follows: (1) no CSL; (2) small amounts of CSL; (3) moderate CSL; and (4) heavy CSL. Differences between protocols were analyzed with Kruskal-Wallis and Mann-Whitney U tests. Friedman and Wilcoxon signed rank tests were used for comparison between each root canal third. SAF resulted in less formation of CSL when compared with the conventional irrigation and EndoVac microcannula (p<0.05). When root canal thirds were analyzed, conventional irrigation and EndoVac groups showed less CSL formation at coronal and middle thirds in comparison to the apical third (p<0.05). In SAF group, there was no difference among the thirds (p>0.05). It may be concluded that an intermediate flush of distilled water, delivered by the SAF system resulted in a better reduction of CSL formation during chemomechanical preparation.

scholarly journals Comparative Evaluation of Cleaning Efficacy using Four Novel Nickel–titanium Rotary Instruments: An in vitro Scanning Electron Microscope Study

2017 ◽  
Vol 18 (12) ◽  
pp. 1135-1143
Author(s):  
Prem P Kar ◽  
Sandhya A Khasnis ◽  
Krisnamurthy H Kidiyoor
Keyword(s):  
Electron Microscope ◽  
Scanning Electron Microscope ◽  
Root Canal ◽  
Nickel Titanium ◽  
Smear Layer ◽  
Layer Removal ◽  
Rotary Instruments ◽  
Significant Difference ◽  
Cleaning Efficacy ◽  
Scanning Electron

ABSTRACT Aim The aim of the study was to compare the cleaning efficacy (debris and smear layer removal) of two multifile rotary systems (MTwo and Silk) and two single-file rotary systems (F6 Skytaper and NeoNiTi). Materials and methods Eighty mesial canals of mandibular first molars were cleaned and shaped using four nickel–titanium (NiTi) rotary instruments to size # 25 and 3% NaOCl and 17% ethylenediaminetetraacetic acid (EDTA). Samples were randomly divided into four equal groups (n = 20) according to instrumentation: Group I, Mtwo; group II, Silk; group III, F6 Skytaper; group IV, NeoNiTi. Samples were split longitudinally and examined under scanning electron microscope (SEM) for debris and smear layer removal in coronal, middle, and apical thirds of each root canal. Results F6 skytaper and Mtwo groups showed significantly higher debris removal than Silk and NeoNiTi groups in apical third of root canal as well as when compared with NeoNiTi group in middle third. F6 Skytaper group showed significantly higher debris and smear layer removal than Silk group in coronal third. There was statistically significant difference among all thirds of root canal in terms of debris removal in Silk and NeoNiTi groups. There was statistically significant difference among all thirds of root canal in F6 Skytaper and NeoNiTi groups in terms of smear layer removal. Conclusion F6 Skytaper single-file rotary instrumentation showed the maximum cleaning efficacy followed by Mtwo multifile rotary instrumentation in all thirds of root canal. Clinical significance F6 Skytaper rotary instrument is most efficient followed by Mtwo rotary instrument among all rotary instruments. How to cite this article Kar PP, Khasnis SA, Kidiyoor KH. Comparative Evaluation of Cleaning Efficacy using Four Novel Nickel-titanium Rotary Instruments: An in vitro Scanning Electron Microscope Study. J Contemp Dent Pract 2017;18(12):1135-1143.

scholarly journals A scanning electron microscopy study of root surface smear layer removal after topical application of EDTA plus a detergent

2005 ◽  
Vol 13 (3) ◽  
pp. 247-252 ◽  
Author(s):  
José Eduardo Cezar Sampaio ◽  
Flávia Pavan Campos ◽  
Gibson Luiz Pilatti ◽  
Letícia Helena Theodoro ◽  
Fábio Renato Manzolli Leite
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Topical Application ◽  
Microscopy Study ◽  
Root Surface ◽  
Smear Layer ◽  
Control Group ◽  
Human Teeth ◽  
Layer Removal ◽  
Scanning Electron

The aim of the present study was to compare root surface smear layer removal following topical application of EDTA and EDTA-T (Texapon). Extracted human teeth had their cementum removed and were mechanically scaled. A total of 220 root specimens were obtained and were randomly assigned to the following groups: I-saline solution (control), II-EDTA; III-EDTA-T. Groups II and III specimens were assigned to different EDTA gel concentrations: 5%, 10%, 15%, 20% and 24%. Smear layer removal score was assessed for each specimen by scanning electron microscopy. The results demonstrated that EDTA and EDTA-T gel led to a higher root surface smear layer removal when compared to the control group. The 5% EDTA gel also showed a higher smear layer removal than the 15%, 20% and 24% EDTA gels (p<0.05). No difference could be found between the different concentrations of EDTA-T gels tested (p>0.05). EDTA gels had statistically significantly lower smear layer scores than the EDTA-T gels for the 5% and 10% concentrations. The results suggested that topical application of EDTA or EDTA-T gel led to significant smear layer removal of the mechanically treated root surfaces. The addition of a detergent to the EDTA gel formula did not improve smear layer removal of the root surface.

scholarly journals Scanning electron microscopy investigation of canal cleaning after canal preparation with nickel titanium files

2010 ◽  
Vol 138 (9-10) ◽  
pp. 551-556
Author(s):  
Tatjana Brkanic ◽  
Ivana Stojsin ◽  
Karolina Vukoje ◽  
Slavoljub Zivkovic
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Root Canal ◽  
Smear Layer ◽  
Root Canal Preparation ◽  
Human Teeth ◽  
Significant Difference ◽  
Cleaning Efficacy ◽  
Scanning Electron

Introduction. Root canal preparation is the most important phase of endodontic procedure and it consists of adequate canal space cleaning and shaping. In recent years, rotary instruments and techniques have gained importance because of the great efficacy, speed and safety of the preparation procedure. Objective. The aim of this research was to investigate the influence of different NiTi files on the canal wall cleaning quality, residual dentine debris and smear layer. Methods. The research was conducted on extracted human teeth in vitro conditions. Teeth were divided in 7 main groups depending on the kind of instruments used for root canal preparation: ProTaper, GT, ProFile, K-3, FlexMaster, hand ProTaper and hand GT. Root canal preparation was accomplished by crown-down technique. Prepared samples were assessed on scanning electron microscopy JEOL, JSM-6460 LV. The evaluation of dentine debris was done with 500x magnification, and the evaluation of smear layer with 1,000 times magnification. Quantitive assessment of dentine debris and smear layer was done according to the criteria of Hulsmann. Results. The least amount of debris and smear layer has been found in canals shaped with ProFile instruments, and the largest amount in canals shaped with FlexMaster instruments. Canal cleaning efficacy of hand GT and ProTaper files has been similar to cleaning efficacy of rotary NiTi files. Statistic analysis has shown a significant difference in amount of dentine debris and smear layer on the canal walls between sample groups shaped with different instruments. Conclusion. Completely clean canals have not been found in any tested group of instruments. The largest amount of debris and smear layer has been found in the apical third of all canals. The design and the type of endodontic instruments influence the efficacy of the canal cleaning.

scholarly journals Scanning electron microscopy of root canal walls after removing the smear layer

2003 ◽  
Vol 50 (2) ◽  
pp. 65-69 ◽  
Author(s):  
Jovanka Gasic ◽  
Dragica Dacic-Simonovic ◽  
Goran Radicevic ◽  
Aleksandar Mitic ◽  
Goran Stojilkovic ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Root Canal ◽  
Smear Layer ◽  
Layer Removal ◽  
Effective Removal ◽  
Time Periods ◽  
Dentine Surface ◽  
Scanning Electron

The purpose of this study was to investigate ultrastructurally the effect of smear layer removal by applying different root canal irrigances: 3% HO2, 4% NaOCl and 15% Na-EDTA (in combination with 3% HO2, 4% NaOCl) and establish the appearance of root canal dentine surface after treatment with 15% Na-EDTA for different time periods(1 min and 5 min) using additional irrigant 3% HOor 4% NaOCl. 22 22 Teeth with single and double canals extracted for orthodontic reasons, were used in this study. After instrumentation, coronal and apical parts were removed. Middle parts were cut longitudinally into two equal segments. The specimens were grouped and treated: with HO2, NaOCl, and with combination of Na-EDTA+ HO2 and Na-EDTA+NaOCl where the irrigation with 2 Na-EDTA lasted 1 or 5 minutes. Control segments were enlarged and irrigated with distillate water to obtain the smear layer. The results obtained showed that irrigation with HOand NaOCl did 22 not remove the smear layer, one-minute application of 15% Na-EDTA followed by 4% NaOCl showed that the smear layer was completely removed. Using of 3% HOafter Na-EDTA over the same period of time obtained 22 less "clear" surfaces. Five-minute application of Na-EDTA caused excessive peritubular and intertubular dentinal erosion. It is concluded that for effective removal of smear layer with Na- EDTA, this procedure should not last longer than 1 minute and should be followed by NaOCl.

scholarly journals Evaluation of four final irrigation protocols for cleaning root canal walls

2020 ◽  
Vol 12 (1) ◽  
Author(s):  
Qiang Li ◽  
Qian Zhang ◽  
Xiaoying Zou ◽  
Lin Yue
Keyword(s):  
Root Canal ◽  
Laser Scanning ◽  
Laser Scanning Microscopy ◽  
Smear Layer ◽  
Confocal Laser ◽  
Bacterial Survival ◽  
Bacterial Inhibition ◽  
Layer Removal ◽  
Significant Difference ◽  
Canal Systems

Abstract The aim of this study was to compare the efficiency of four final irrigation protocols in smear layer removal and bacterial inhibition in root canal systems. Thirty roots inoculated with Enterococcus faecalis were prepared with ProTaper Universal files. The teeth were disinfected by conventional needle irrigation, sonic agitation using the EndoActivator device, passive ultrasonic irrigation, or an M3 Max file. Teeth with no root canal preparation served as blank controls for the establishment of the infection baseline. Teeth with preparation but no final irrigation served as a post-instrumentation baseline. After the final irrigation, the teeth were sectioned in half. One half of each tooth was examined by scanning electron microscopy (SEM) to assess smear layer removal using a five-point scale. The other half was examined by confocal laser scanning microscopy (CLSM) using the LIVE/DEAD BackLight bacterial viability kit to evaluate the depth of bacterial survival in dentinal tubules. SEM analysis revealed no significant difference in smear layer removal throughout the whole canal among the EA, PUI, and M3 Max groups (P > 0.05). CLSM revealed that PUI achieved the greatest bacterial inhibition depth in the coronal ((174.27 ± 31.63) μm), middle ((160.94 ± 37.77) μm), and apical ((119.53 ± 28.49) μm) thirds of the canal (all P < 0.05 vs. other groups). According to this comprehensive SEM and CLSM evaluation, PUI appears to have the best infection control ability in root canal systems.

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