scholarly journals Comparative Phytochemical Profiling of Garlics (Allium sativum L.) and Onion (Alium cepa L.)

2021 ◽  
pp. 7-17
Author(s):  
Siti Nor Aisyah Mohd Zawawi ◽  
Nurul Izzati Osman ◽  
Ibtisam Abdul Wahab ◽  
Hannis Fadzillah Mohsin
Keyword(s):  
Thin Layer ◽  
Ethyl Acetate ◽  
Thin Layer Chromatography ◽  
Reducing Sugars ◽  
Pharmaceutical Chemistry ◽  
Chemical Compositions ◽  
Phytochemical Screening ◽  
Black And White ◽  
Ethanolic Extracts ◽  
Layer Chromatography

Aims: This research aims to perform the extraction of garlic samples and onion as well as to carry out qualitative phytochemical screening by using Thin Layer Chromatography (TLC) and phytochemical analyses for the detection of carbohydrates, flavonoids, and alkaloids.  Study Design:  Thin Layer Chromatography (TLC) and some preliminary qualitative phytochemical tests to detect the presence of carbohydrates and reducing sugars, flavonoids,   and alkaloids   were   carried   out   to   compare   and   identify   the chemical compositions in black and white garlic as well as onion. Place and Duration of Study: Pharmaceutical Chemistry Department laboratory, Faculty of Pharmacy Universiti Teknologi MARA Puncak Alam. The whole study was conducted and completed in 12 months. Methodology: Black garlic (BG) is derived from raw white garlic (WG) that is modified by treating it with highly controlled temperature and humidity. In addition to the black and white garlic powder samples, this comparative study was also performed on smoked garlic and onion. The extraction of black garlic (BG), white garlic (WG), crushed smoked garlic (CSG), and onion was performed by using ethanol and ethyl acetate. The comparative phytochemical profiling was conducted by using TLC and qualitative phytochemical analyses were done using standard methods. Results: The presence of amino acid in the ethanolic extracts of black garlic (BG), crushed smoked garlic (CSG), and onion were detected. Ethyl acetate extracts might contain triterpene. Phytochemical screening tests showed the presence of reducing sugars and alkaloids in ethanolic extracts of black garlic (BG), while white garlic (WG) contains flavonoids, alkaloids, and a trace amount of carbohydrates. Both extracts of onion showed the presence of carbohydrate, reducing sugars and flavonoids. Meanwhile alkaloids are only detected in ethanolic extract of onion. Conclusion: The extracts of garlics and onion samples in this study had shown appreciable presence of some important phytochemical compounds in Allium species such as amino acids, flavonoids, alkaloids and carbohydrates.

scholarly journals PHARMACOGNOSTIC STANDARDIZATION, PRELIMINARY PHYTOCHEMICAL SCREENING AND TLC FINGERPRINTING OF THE BARK OF CASCABELA THEVETIA L.

2019 ◽  
pp. 125-130
Author(s):  
Neelutpal Gogoi ◽  
Biman Bhuyan ◽  
Trinayan Deka
Keyword(s):  
Thin Layer ◽  
Ethyl Acetate ◽  
Petroleum Ether ◽  
Thin Layer Chromatography ◽  
Water Soluble ◽  
Phytochemical Analysis ◽  
Alcoholic Extract ◽  
Phytochemical Screening ◽  
Cork Cell ◽  
Layer Chromatography

Objectives: In this study, systematic pharmacognostic study and preliminary phytochemical screening of the bark of Cascabela thevetia L. were carried out. Methods: The selected plant part was collected, processed and stored in an airtight container. From the bark different pharmacognostic studies like macroscopic and microscopic evaluation, physicochemical parameters, fluorescence analysis were done. Powdered bark was successively extracted by petroleum ether, chloroform, ethyl acetate, and methanol using a Soxhlet apparatus and finally macerated with the hydro-alcoholic solvent system (30:70). The preliminary phytochemical analysis and thin layer chromatography of the extracts were done to find the nature and number of the different phytoconstituents present. Results: Transverse microscopy reveals the presence of crystal oxalate, cork cell, starch granules, vascular bundle, phloem fiber, parenchyma cells, and collenchyma cells. Powder microscopy also showed the presence of cork cell, fiber and calcium oxalate crystal. Results obtained in different physicochemical analysis like total ash, acid insoluble ash, water soluble ash, alcohol-soluble extractive, water-soluble extractive, and moisture content were 8.67%, 0.83%, 5.33%, 4.53%, 12.27%, and 7.83% respectively. Phytochemical analysis showed the presence of alkaloid, flavonoid, triterpenoid, phytosterol, tannin, saponin, anthraquinone, carbohydrate and fatty acid in the different extracts. TLC (Thin Layer Chromatography) study revealed 4 spots in petroleum ether, chloroform, ethyl acetate, and methanol extracts and 3 spots in the Hydro-alcoholic extract with different solvent systems. Conclusion: The results obtained from the study will provide a reliable basis for identification, purity, and quality of the plant.

scholarly journals Identifikasi Senyawa Metabolit Sekunder pada Durian (Durio zibethinus Murr)

2019 ◽  
Vol 1 (1) ◽  
pp. 1-6
Author(s):  
Aji Suteja ◽  
Emmy Harso Kardhinata ◽  
Rosliana Lubis
Keyword(s):  
Thin Layer ◽  
Ethyl Acetate ◽  
Experimental Method ◽  
Thin Layer Chromatography ◽  
Secondary Metabolite ◽  
Phytochemical Screening ◽  
Screening Analysis ◽  
Durio Zibethinus ◽  
Rf Values ◽  
Layer Chromatography

This study aims to determine how to identify the types of secondary metabolite compounds contained in durian leaves. The study was conducted by an experimental method by identifying secondary metabolite compounds on durian leaves using phytochemical screening. The sample criteria used were the leaf buds and all the leaf strands (except the leaf bones). The results of the study showed that of the three types of durian leaves namely copper, baskets and Sp A. There are several types of secondary metabolite compounds including alkaloids, steroids, and terpenoids. Phytochemical screening analysis was carried out using thin layer chromatography by showing RF values on three types of durian leaves using methanol and ethyl acetate solvents in a ratio of 3: 1. RF value on copper durian is 0.97, bakul durian is 0.95 and Sp durian. A 0.94.

scholarly journals Preliminary Phytochemical Screening and Thin Layer Chromatography Analysis of Stem Bark Extracts of African Mistletoe Parasitic on Vitellaria paradoxa, Piliostigma thonningii and Combretum fragrans

2019 ◽  
pp. 1-6
Author(s):  
T. Agber Cyprian ◽  
Shaakaa Sewuese ◽  
Linus U. Akacha
Keyword(s):  
Thin Layer ◽  
Ethyl Acetate ◽  
Thin Layer Chromatography ◽  
Heart Diseases ◽  
Stem Bark ◽  
Phytochemical Screening ◽  
Vitellaria Paradoxa ◽  
Chromatography Analysis ◽  
Plant Hosts ◽  
Layer Chromatography

Aim: Mistletoes are highly utilized in traditional medicine to treat different kinds of diseases such as heart diseases, diabetes and malaria, among others. The chemistry of African mistletoe is not sufficiently documented. This paper is therefore, aimed at determining the phytochemicals present in the crude extracts of mistletoe parasitic on plants that are commonly seen as hosts. Study Design: This study was designed to compare the phytochemical profiles of mistletoe stem barks obtained from different plant hosts. Place and Duration of Study: Department of Chemistry, University of Agriculture, Makurdi, Benue State Nigeria, between August and September, 2018.   Methodology: Powdered stem bark of mistletoe was extracted successively with hexane, ethyl acetate and methanol. Preliminary phytochemical screening was carried out on the extracts. Thin layer chromatography (TLC) was carried out on silica gel precoated plates in 9:1 (hexane/ethyl acetate), 1:1 (hexane/ethyl acetate), and 7:3 (ethyl acetate/methanol) mobile phases for hexane, ethyl acetate and methanol extracts respectively. Results: The study revealed the presence of secondary metabolites such as alkaloids, flavonoids, tannins/phenols, cardiac glycosides, steroids and triterpenoids. It was evident from TLC analysis that mistletoes from various plant hosts contain similar chemical profile. Conclusion: We therefore debunk the claim by some herbalists that medicinal values of mistletoes vary due to host plant. This is the first time a study of this kind is reported on mistletoe parasitic on Vitellaria paradoxa Pilostigma thonningii, Combretum fragrans.

scholarly journals Identifikasi Senyawa Tanin Pada Daun Kemuning (Murraya panicullata L. Jack) Dengan Berbagai Jenis Pelarut Pengekstraksi

2017 ◽  
Vol 2 (1) ◽  
pp. 29-32
Author(s):  
Galuh Gondo Kusumo ◽  
M.A. Hanny Ferry Fernanda ◽  
Heppy Asroriyah
Keyword(s):  
Thin Layer ◽  
Ethyl Acetate ◽  
Thin Layer Chromatography ◽  
Secondary Metabolite ◽  
Crude Extract ◽  
Screening Test ◽  
Human Life ◽  
Phytochemical Screening ◽  
Murraya Paniculata ◽  
Layer Chromatography

ABSTRAKKemuning (Murraya paniculata L. Jack) adalah salah satu kekayaan alam yang memiliki banyak manfaat bagi kehidupan manusia. Tanin merupakan salah satu metabolit sekunder dari kemuning yang dapat digunakan sebagai anti diare dan pelangsing. Ekstrak kemuning didapatkan dari maserasi menggunakan tiga pelarut berbeda, yaitu metanol, etanol dan etil asetat. Tannin kemudian dipisahan dari ekstrak dengan menggunakan kromatografi lapis tipis (KLT) dengan berbagai jenis pelarut. Hasil nalisis menunjukkan bahwa pelarut terbaik untuk mengekstraksi tanin adalah metanol dengan perolehan 23,6989 g (31,59%). Skrining fitokimia yang dilakukan menggunakan dua reagen yang berbeda menunjukkan hasil yang positif mengandung tanin. Eluenterbaik untuk analisa tanin pada penelitian ini adalah dengan n-heksan-etil asetat (6 : 4) dengan nilai Rf sebesar 0,62.Kata Kunci : kemuning (Murraya paniculata L. Jack), tannin, kromatografi lapis tipis (KLT)ABSTRACTOrange Jessamine (Murraya paniculata L. Jack) is one of the natural treasures which has many benefits for human life. Tannin is one of secondary metabolite of orange jessamine that can be used as antidiarrhoeal and body slimming. It was obtained by maceration using 3 different solvents, such as : methanol, ethanol, and ethyl acetate. Tannins was separated from crude extract using thin layer chromatography (TLC) in different type of eluent. The analysis showed that the best solvent to extract tannin is methanol that produce of 23.6989 g (31.59%). The phytochemical screening test of the two reagents shows positif result contain tannin compound. The best eluent in this study aimed is n-hexane: ethyl acetate (6 : 4) with tannin Rf value of 0.62.Keywords: Orange jessamine (Murraya paniculata L. Jack), tannin, maceration, thin layer chromatography

Direct Fluorescent Detection of Organothiophosphorus Pesticides and Some of Their Sulfur-containing Breakdown Products After Thin Layer Chromatography

1967 ◽  
Vol 50 (5) ◽  
pp. 1088-1098
Author(s):  
Mohamed Tawfik H Ragab
Keyword(s):  
Thin Layer ◽  
Ethyl Acetate ◽  
Thin Layer Chromatography ◽  
Ultraviolet Light ◽  
Congo Red ◽  
Fluorescent Detection ◽  
Bromine Vapor ◽  
Layer Chromatography ◽  
Sulfur Containing ◽  
Dark Blue

Abstract A rapid, simple, convenient, and widely applicable method for the direct fluorescent detection of organothiophosphorus pesticides and some postulated breakdown products of these compounds is presented. The coinpounds were spotted on thin layer chromatographic sheets, developed in ethyl acetate :nhexane, and made visible by exposure to bromine vapor followed by spraying with ferric chloride and 2-(o-hydroxy phenyl) benzoxazole. Of the 47 compounds tested, 32 compounds produced fluorescent blue spots vinder longwave ultraviolet light; these consisted of 25 organothiophosphorus pesticides, 5 sulfur-containing breakdown products, and phosphoric and hypophosphorous acids. A superimposed Congo red spray destroyed the fluorescence and resulted in dark blue spots against a red backgrovind. The sensitivity of this method is in the range of 0.2 to 5.0 μg, depending on the specific compound.

scholarly journals PHYTOCHEMICAL INVESTIGATION OF THE MEDICINAL PLANT MORICANDIA ARVENSIS L. FROM ALGERIAN SAHARA

2018 ◽  
Vol 11 (5) ◽  
pp. 450 ◽  
Author(s):  
Abdelaziz Berreghioua ◽  
Abdelkrim Cheriti
Keyword(s):  
Medicinal Plant ◽  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Acetone Extract ◽  
Phytochemical Screening ◽  
Algerian Sahara ◽  
Phytochemical Investigation ◽  
Moricandia Arvensis ◽  
Chemical Groups ◽  
Layer Chromatography

Objective: The aim of this research was to isolate and identify flavonoids extracted from the leaves of Moricandia arvensis.Methods: The phytochemical screening reaction and thin-layer chromatography have been used to characterize the chemical groups, before they were identified by nuclear magnetic resonance.Results: The leaves contain essentially flavonoids, tannins, cardenolides, saponins, and alkaloids. The phytochemical investigation of the water-acetone extract led to the isolation of five flavonoids derivatives, namely: 5,7-dihydroxy-3,6,4’-trimethoxyflavone (1); 5,7,4’-trihydroxy- 3,6,8,3’-tetramethoxyflavone (2); 3,3’,4’, 5,7- pentahydroxy flavanone (3); 3-glucosyl 3’,4’,5,7 tetrahydroxy flavonol (4); and kaempférol-3- digalactopyranoside (5). The structures of 1–5 were identified by comparison of their spectral data with those reported in the literature.Conclusion: In this work, it was possible to isolate and identify five flavonoids after fractionation of the hydroacetone extract from the leaves of the medicinal plant M. arvensis.

scholarly journals Phytochemical Investigation of the Aerial Part of Iraqi Convolvulus arvensis

2020 ◽  
Vol 29 (2) ◽  
pp. 62-69
Author(s):  
Mustafa H. Alwan ◽  
Maha N. Hamad
Keyword(s):  
Liquid Chromatography ◽  
Thin Layer ◽  
Ethyl Acetate ◽  
Thin Layer Chromatography ◽  
Mass Spectroscopy ◽  
Morning Glory ◽  
Convolvulus Arvensis ◽  
Aerial Parts ◽  
Phytochemical Investigation ◽  
Layer Chromatography

  Convolvulus arvensis is a species of bindweed that is rhizomatous and is in the morning glory family (Convolvulaceae) native to Europe and Asia. The plant is naturally grown in Iraq. The plant was reported to be used in traditional medicine from as early as 1730s. The Aerial parts of Convolvulus arvensis were macerated in 80% ethanol for 6 days. The concentrated extract was partitioned with n-hexane, chloroform, ethyl acetate- and n-butanol successively. The n-hexane and ethyl acetate, fractions were examined for the presence of phytochemicals by thin layer chromatography and high performance liquid chromatography and its steroid and flavonoid contents were investigated. Stigmasterol was isolated from n-hexane fraction and identified by liquid chromatography/mass spectroscopy. Rutin was isolated from the ethyl acetate fraction and identified by liquid chromatography/mass spectroscopy. The aim is to examine the phytochemical constituents of the aerial parts of Convolvulus arvensis, literature survey available so far revealed that there were no studies about the phytochemical investigation for Convolvulus arvensis in Iraq.  Different chromatographic techniques like Thin Layer Chromatography and mass spectroscopy were used and the presence of Stigmasterol and Rutin in aerial parts of Convolvulus arvensis was indicated.  

Sign in / Sign up

Export Citation Format

Share Document