Molecular Docking Evaluation of the Desert Truffles as Potent Antifungal Inhibitors

The research investigated the possible antifungal behavior of forty-four truffles bioactive compounds conducted to investigate the interaction modes of these inhibitors against three different types of the fungal proteins: Candida albicans, Blastomyces dermatitidis, and Ganoderma microsporum. The applied method in contrast to ketoconazole and griseofulvin revealed the possible anti-fungal agents ergosterol, Catechin gallate and rutin. With respect to Candida Albicans, the maximum possible binding energy was ergosterol (-11.75 Kcal/mol), followed then by catechin gallate (-11.46 Kcal/mol) then rutin (-9.90 Kcal/mol). Compared to Blastomyces, Ganoderma microsporum fungal protein with most negative binding energy among other components of the truffle is found to be of a relatively similar behavior for the same compounds. Ergosterol demonstrated the highest binding capacity for dermatitidis, while rutin scored the lowest against Ganoderma microsporum. The possible anti-fungal components of desert truffle have yet to be studied in vitro in the future.

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Thidiazuron, a phenyl-urea cytokinin, inhibits ergosterol synthesis and attenuates biofilm formation of Candida albicans

10.21203/rs.3.rs-780442/v1 ◽

2021 ◽

Author(s):

Harikrishnan Pandurangan ◽

Balamani Arayambath ◽

Vijay Karthik Jayaraman ◽

Kanimozhi Ekambaram ◽

Emad A Ahmed ◽

...

Keyword(s):

Gene Expression ◽

Candida Albicans ◽

Crystal Violet ◽

Biofilm Formation ◽

Reduction Assay ◽

Ergosterol Synthesis ◽

Inhibitory Potential ◽

Anti Fungal ◽

Time Kill

Abstract Candida albicans is a commensal human fungal pathogen that colonizes and develops dental biofilm which cause Oral candidiosis. This study investigates the effects of a new molecule Thidiazuron against the growth and biofilm formation properties of C. albicans. This study applied computational and in vitro approaches such as broth microdilution, SEM, time-kill dynamics, crystal violet assay, XTT reduction assay, ergosterol quantification and quantitative RT PCR analysis of gene expression to validate the growth and biofilm inhibitory potential of thidiazuron against C. albicans. Preliminary molecular docking study revealed potential interaction between thidiazuron and amino acids residues of CYP51. Further in vitro anti-fungal susceptibility test, SEM and time kill analysis revealed anti-fungal potency of thidiazuron in dose and time dependent passion. Crystal violet staining, XTT reduction assay and Acridine Orange staining visually confirmed biofilm inhibitory potential of thidiazuron. Gene expression study shows that thidiazuron treatment down regulated the expression of genes involved in ergosterol synthesis, cell adhesion and hyphae development in C. albicans. This study identified thidiazuron as CYP51 inhibitor and a new antibiofilm agent against C. albicans.

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ANTI-FUNGAL ACTIVITY OF ACALYPHA WILKESIANA: A PRELIMINARY STUDY OF FUNGAL ISOLATES OF CLINICAL SIGNIFICANCE

African Journal of Infectious Diseases ◽

10.21010/ajid.v16i1.4 ◽

2021 ◽

Vol 16 (1) ◽

pp. 21-30

Author(s):

Katibi Oludolapo Sherifat ◽

Aboh Mercy Itohan ◽

Salawu Oluwakayinsola Adeola ◽

Kola -Mustapha Adeola ◽

Olatunji Lawrence Aderemi

Keyword(s):

Candida Albicans ◽

Antifungal Activity ◽

Trichophyton Rubrum ◽

Seborrheic Dermatitis ◽

Ethanol Extract ◽

Microsporum Canis ◽

Plant Materials ◽

Acalypha Wilkesiana ◽

Anti Fungal

Acalypha wilkesiana (AW), a popular medicinal plant has been used in traditional medicine to treat a variety of skin disorders including pityriasis versicolor and seborrheic dermatitis. As a prelude to clinical trials in humans, an experimental study was carried out to determine the spectrum of antifungal activity of 2 variants of the Acalypha wilkesiana plant. Materials and Methods: The ethanol extract and herbal cream formulation of the dried leaves of 2 cultivars (Macrophylla & Hoffmani) of Acalypha wilkesiana were investigated for in-vitro antifungal activity by disc diffusion and micro-broth dilution techniques. Organisms tested were typed cultures of Malassezia furfur, Candida albicans and Trichophyton rubrum; and clinical strains of Microsporum canis and Epidermophyton floccosum. Results: Both cultivars (Macrophylla and Hoffmanii) of the plant showed good activity against all the fungi tested except Microsporum canis (8.0±0.00; 7.00±0.00 mm). The greatest activity was observed against Trichophyton rubrum (22.0±0.00; 24.00±0.00 mm) while Candida albicans showed the least activity (15.0±0.00; 18.00±0.57 mm). The Minimum Inhibitory Concentration (MIC) of the crude extract ranged between 0.25 and 8 mg/ml for all organisms, while that of the herbal cream was 0.31-8mg/ml. The lowest MIC was seen with Candida albicans for both varieties of the plant. The Acalypha wilkesiana Hoffmanii demonstrated a greater activity against Candida albicans and Malassezia furufur than the A. wilkesiana (Macrophylla). Conclusion: This study reveals Acalypha wilkesiana leaf extract has potential for development as a cream that can be used to treat superficial fungal skin infections.

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The in vitro formation of candida albicans biofilms on titanium discs and their susceptibility to an anti-fungal agent

10.5353/th_b4500777 ◽

2005 ◽

Author(s):

Hyden Ng

Keyword(s):

Candida Albicans ◽

Anti Fungal ◽

Candida Albicans Biofilms

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Peptidomimetic Inhibitors of Extracellular Aspartic Proteinases of Candida albicans and Candida tropicalis

Collection of Czechoslovak Chemical Communications ◽

10.1135/cccc19990130 ◽

1999 ◽

Vol 64 (1) ◽

pp. 130-137 ◽

Cited By ~ 3

Author(s):

Kateřina Brožková ◽

Ivana Křížová ◽

Libuše Pavlíčková ◽

Martin Hradilek ◽

Martin Fusek ◽

...

Keyword(s):

Candida Albicans ◽

Binding Energy ◽

Candida Tropicalis ◽

Enzyme Inhibitor ◽

Side Chains ◽

Peptide Substrate ◽

Aspartic Proteinases ◽

Scissile Bond ◽

Protected Peptides

In an attempt to develop effective inhibitors of Candida secreted aspartic proteinases, we have prepared a series of N-protected peptides varying in the type of scissile bond replacement, in the P and P' side chains as well as in the N- and C-terminal modifications. The compounds were tested in vitro with the chromogenic peptide substrate using purified secreted proteinases of C. albicans and C. tropicalis. Our results have confirmed that the binding of inhibitors and their effectiveness is influenced by a number of enzyme-inhibitor interactions. Moreover, factors like solvation/desolvation contribute to the optimal binding energy of the inhibitors.

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Daya Hambat Minyak Atsiri Rimpang Kunyit terhadap Pertumbuhan Candida Albicans In Vitro

Jurnal Vokasi Kesehatan ◽

10.30602/jvk.v4i1.124 ◽

2018 ◽

Vol 4 (1) ◽

pp. 1

Author(s):

Fitri Nadifah ◽

Nurlaili Farida Muhajir ◽

Fitri Retnoningsih

Keyword(s):

Candida Albicans ◽

Essential Oil ◽

Inhibition Zone ◽

Diffusion Method ◽

Inhibition Activity ◽

Significant Difference ◽

Oil Concentration ◽

Anti Fungal ◽

One Way Anova

Abstract: Inhibition Activity Of Turmeric Essential Oil Against The Growth Of Candida Albicans. Turmeric has rhizomes which contain essential oils and curcuminoid that has anti-fungal activity. The purpose of this research was to know the influence of essential oil of turmeric Rhizome (Curcuma domestica Val.) against the growth of Candida albicans in vitro. This was an experimental research with diffusion method. Essential oil obtained through distillation methods and the concentration used was 20%, 40%, 60%, 80% and 100%. The treatment was repeated three times. Anova test was used. Inhibition zone is formed at a concentration of 20%, 40%, 60%, 80%, 100% were 6,16 mm, 6,50 mm, 6,66 mm, 7,00 mm and 7.83 mm, respectively Results in analysis by one way ANOVA showed there was a significant difference in the variance of essential oil concentration against the inhibition zone (p< 0.05).Abstrak: Daya Hambat Minyak Atsiri Rimpang Kunyit Terhadap Pertumbuhan Candida Albicans In Vitro. Kunyit merupakan salah satu rimpang yang diketahui memiliki kandungan minyak atsiri serta curcuminoid yang bersifat anti jamur. Tujuan penelitian ini untuk mengetahui pengaruh minyak atsiri rimpang kunyit (Curcuma domestica Val.) terhadap pertumbuhan Candida albicans secara in vitro. Jenis penelitian ini adalah penelitian eksperimental dengan metode difusi cara sumuran. Minyak atsiri didapatkan melalui metode destilasi dan konsentrasi yang digunakan adalah 20%, 40%, 60%, 80% dan 100%. Perlakuan diulang sebanyak tiga kali. Hasil penelitian di analisis dengan Anova. Hasil zona hambat yang terbentuk pada konsentrasi minyak atsiri 20%, 40%, 60%, 80%, 100% berturut-turut adalah 6,16 mm, 6,50 mm, 6,66 mm, 7,00 mm, dan 7,83 mm. Hasil analisis dengan one way anova menunjukkan terdapat perbedaan yang signfikan pada berbagai konsentrasi minyak atsiri terhadap zona hambat yang dihasilkan (p<0,05). Dari penelitian ini dapat disimpulkan bahwa berbagai konsentrasi minyak atsiri rimpang kunyit dapat menghambat pertumbuhan Candida albicans. Kemampuan ini berpotensi dikembangkan lebih lanjut sebagai alternatif dalam pengobatan terhadap penyakit karena infeksi Candida albicans.

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Susceptibility in vitro of clinical Candida albicans isolates to the selected azoles

Current Issues in Pharmacy and Medical Sciences ◽

10.2478/cipms-2021-0015 ◽

2021 ◽

Vol 0 (0) ◽

Author(s):

Bartlomiej Pawlicki ◽

Anna Biernasiuk ◽

Monika Jonczyk ◽

Anna Malm

Keyword(s):

Candida Albicans ◽

Minimum Inhibitory Concentration ◽

Fungal Infection ◽

Inhibitory Concentration ◽

Invasive Disease ◽

Yeast Strains ◽

Resistance Rates ◽

Anti Fungal ◽

Mic Minimum Inhibitory Concentration

Abstract Candida spp. is the most prevalent cause of fungal infection worldwide, and their increasing resistance to anti-fungal agents, especially to azoles, has become problematic. The aim of this work was to establish the susceptibility to fluconazole, itraconazole, voriconazole and posaconazole of 50 clinical C. albicans isolates from hematooncological patients. This has been evaluated using the following parameters: MIC (Minimum Inhibitory Concentration), MIC50 (MIC required to inhibit the growth of 50% of organisms), as well as MIC90 (MIC required to inhibit the growth of 90% of organisms). Susceptibility of the studied clinical isolates to all azoles was high, being 86% for itraconazole, 90% for fluconazole and posaconazole and 92% for voriconazole. The resistance rates ranged from 8% (voriconazole), to 12% (itraconazole). The emergence of azole-resistant yeast strains creates a necessity to determine and monitor the sensitivity of the isolated Candida spp., including C. albicans, especially in patients predisposed to life-threating fungal invasive disease.

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Penatalaksanaan infeksi Candida tropicalis pada penderita median rhomboid glossitis The management of infection of Candida tropicalis at median rhomboid glossitis patient

Journal of Dentomaxillofacial Science ◽

10.15562/jdmfs.v10i1.246 ◽

2011 ◽

Vol 10 (1) ◽

pp. 13

Author(s):

Cane Lukisari ◽

Kus Harijanti

Keyword(s):

Candida Albicans ◽

Candida Tropicalis ◽

Oral Candidiasis ◽

Good Prognosis ◽

Broad Spectrum Antibiotic ◽

Trigger Factors ◽

Paper Report ◽

Medium Level ◽

Anti Fungal

Oral candidiasis is oral mucosal infectious caused by Candida albicans or non-candida albicans candida (NCAC).Each Candida species infection has same clinical manifestation, the different only in invasive nature and antifungiresponse. C.tropicalis is most virulence NCAC because the most adherence ability to epithelial cells in vitro as wellas medium level proteinase secretion. In addition, C.topicalis is the second most common colony on human. A studyreported of C.tropicalis as causa of 16% hospitalized oral candidiasis, anti fungal drugs and systemic antibioticwas known as trigger factors. This paper report and discuss oral candidiasis due to C.tropicalis in patient, adultmale, with median rhomboid glossitis. The patient also used broad spectrum antibiotic and topical corticosteroidwith imprecise dose. The management of this case was terminated antibiotic and corticosteroid, oral hygieneoptimalization and was given 0.25% chlorhexidine gluconate. However, oral hygiene optimalization, predispositionfactors elimination, and, precise therapy would give a good prognosis.

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The Effect of Three Different Disinfectant Materials on Polyether Impressions by Spray Method

World Journal of Dentistry ◽

10.5005/jp-journals-10015-1161 ◽

2012 ◽

Vol 3 (3) ◽

pp. 229-233

Author(s):

Ehsan Ghasemi ◽

Hamid Badrian ◽

Nafiseh Hosseini ◽

Navid Khalighinejad

Keyword(s):

Staphylococcus Aureus ◽

Pseudomonas Aeruginosa ◽

Candida Albicans ◽

Sodium Hypochlorite ◽

P Value ◽

Plastic Bags ◽

Spray Method ◽

Significant Difference ◽

Different Types

ABSTRACT Background Dentists, dental equipments and dental laboratories are exposed to different types of pathogenic microorganisms. The aim of this study is to investigate the effect of three different types of disinfectant agents: Sodium hypochlorite 0.525%, Epimax and Deconex, on polyether impressions after 5 and 10 minutes. Materials and methods In this in vitro experimental study, 66 circular samples of polyether impression material of 1 cm diameter and 2 mm thickness were contaminated with Staphylococcus aureus (ATCC29213) Pseudomonas aeruginosa (ATCC27853) and Candida albicans fungus (PTCC5027). Except for control samples, all of them were disinfected with sodium hypochlorite 0.525, Deconex and Epimax by way of spraying. Afterward, they kept in plastic bags with humid rolled cotton for 5 and 10 minutes. In order to isolate bacteria, the samples were immersed in 2% trypsin for 1 hour and then the solution was diluted with normal salin in portion of 1, 1/2, and 1/4. The trypsin suspensions were transferred to culture plates and the number of colonies was counted after 24 and 48 hours for bacteria and after 72 hours for fungus. For data analysis Mann-Whitney statistical test was used (α = 0.05). Results Epimax and sodium hypochlorite thoroughly eliminated Candida albicans as time elapsed from 5 to 10 minutes; however, the other two microorganisms were not eliminated completely. Deconex was completely efficient for all microorganisms when immersion time was enhanced from 5 to 10 minutes. There was a significant difference between efficacy of Deconex-sodium hypochlorite and sodium hypochlorite-Epimax for Pseudomonas aeruginosa in 5-minute immersion (p-value:0/046) Moreover, Deconex and sodium hypochlorite were significantly different for Staphylococcus aureus (p-value:0/046) and Pseudomonas aeruginosa in 10-minute immersion (p < 0.05). Conclusion According to the results of this study, none of the three disinfection materials could eradicate three different kinds of microorganisms in 5 minutes, but in 10 minutes, Deconex could completely eradicate all microorganisms compared to other disinfection agents, which is a good indicator for high efficacy of this agent in disinfecting polyether impressions. How to cite this article Ghasemi E, Badrian H, Hosseini N, Khalighinejad N. The Effect of Three Different Disinfectant Materials on Polyether Impressions by Spray Method. World J Dent 2012;3(3):229-233.

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