scholarly journals Daya Hambat Minyak Atsiri Rimpang Kunyit terhadap Pertumbuhan Candida Albicans In Vitro

2018 ◽  
Vol 4 (1) ◽  
pp. 1
Author(s):  
Fitri Nadifah ◽  
Nurlaili Farida Muhajir ◽  
Fitri Retnoningsih
Keyword(s):  
Candida Albicans ◽  
Essential Oil ◽  
Inhibition Zone ◽  
Diffusion Method ◽  
Inhibition Activity ◽  
Significant Difference ◽  
Oil Concentration ◽  
Anti Fungal ◽  
One Way Anova

Abstract: Inhibition Activity Of Turmeric Essential Oil Against The Growth Of Candida Albicans. Turmeric has rhizomes which contain essential oils and curcuminoid that has anti-fungal activity. The purpose of this research was to know the influence of essential oil of turmeric Rhizome (Curcuma domestica Val.) against the growth of Candida albicans in vitro. This was an experimental research with diffusion method. Essential oil obtained through distillation methods and the concentration used was 20%, 40%, 60%, 80% and 100%. The treatment was repeated three times. Anova test was used. Inhibition zone is formed at a concentration of 20%, 40%, 60%, 80%, 100% were 6,16 mm, 6,50 mm, 6,66 mm, 7,00 mm and 7.83 mm, respectively Results in analysis by one way ANOVA showed there was a significant difference in the variance of essential oil concentration against the inhibition zone (p< 0.05).Abstrak: Daya Hambat Minyak Atsiri Rimpang Kunyit Terhadap Pertumbuhan Candida Albicans In Vitro. Kunyit merupakan  salah satu rimpang yang diketahui memiliki kandungan minyak atsiri serta curcuminoid yang bersifat anti jamur. Tujuan penelitian ini untuk mengetahui pengaruh minyak atsiri rimpang kunyit (Curcuma domestica Val.) terhadap pertumbuhan Candida albicans secara in vitro. Jenis penelitian ini adalah penelitian eksperimental dengan metode difusi cara sumuran. Minyak atsiri didapatkan melalui   metode destilasi dan konsentrasi  yang digunakan adalah 20%, 40%, 60%, 80% dan 100%. Perlakuan diulang sebanyak tiga kali. Hasil penelitian di analisis dengan Anova. Hasil zona hambat yang terbentuk pada konsentrasi minyak atsiri 20%, 40%, 60%, 80%, 100% berturut-turut adalah 6,16 mm, 6,50 mm, 6,66 mm, 7,00 mm,  dan 7,83 mm. Hasil analisis dengan one way anova menunjukkan terdapat perbedaan yang signfikan pada berbagai konsentrasi minyak atsiri terhadap zona hambat yang dihasilkan (p<0,05). Dari penelitian ini dapat disimpulkan bahwa berbagai konsentrasi minyak atsiri rimpang kunyit dapat menghambat pertumbuhan Candida albicans. Kemampuan ini berpotensi dikembangkan lebih lanjut sebagai alternatif dalam pengobatan terhadap penyakit karena infeksi Candida albicans.

scholarly journals The Antifungal Effect of WHO Hand Sanitizer to Candida Albicans in Improving Personal Hygiene

2020 ◽  
Vol 1 (2) ◽  
pp. 7
Author(s):  
Dyah Widiastuti ◽  
Ihda Zuyina Zuyina Ratna Sari ◽  
Isya Fikria Kalimah ◽  
Endang Setiani
Keyword(s):  
Candida Albicans ◽  
Inhibition Zone ◽  
World Health ◽  
Personal Hygiene ◽  
Diffusion Method ◽  
Zone Formation ◽  
Hand Sanitizer ◽  
Fungal Activity ◽  
Significant Difference ◽  
Anti Fungal

Hand hygiene is one of the important factors that determine a person's health status. Hands are easily contaminated by microbes from the environment such as bacteria, viruses, and fungi through direct contact. Hand sanitizer is a type of media that can be used to clean hands from disease-causing microbes other than soap. Hand sanitizers are widely used by the community because they are considered more practical to use. This study aims to determine the anti-fungal activity of the hand sanitizer recommended by the World Health Organization (WHO) against Candida albicans. Anti-fungal activity was tested using the well diffusion method. The results showed that the higher the concentration of the hand sanitizer, the bigger the inhibition zone formed. The statistical test results obtained a significance value of p <0.05 at the variation of the concentration of hand sanitizer 50%, 75%, 100%, and treatment control. These results indicate that there is a significant difference between the hand sanitizer treatment and control of the inhibition zone formation in C. albicans. The WHO recommended hand sanitizer has medium inhibitory power against C. albicans bacteria.

scholarly journals Antifungal Activity of the Essential Oil of Echinops kebericho Mesfin: An In Vitro Study

2020 ◽  
Vol 2020 ◽  
pp. 1-7
Author(s):  
Tamirat Bekele Beressa ◽  
Serawit Deyno ◽  
Paul E. Alele
Keyword(s):  
Candida Albicans ◽  
Antifungal Activity ◽  
Essential Oil ◽  
Cryptococcus Neoformans ◽  
Antioxidant Properties ◽  
Pathogenic Fungi ◽  
Diffusion Method ◽  
Potential Candidate ◽  
Standard Drug

Background. Echinops kebericho is an endemic medicinal plant in Ethiopia widely used in the treatment of infectious and noninfectious diseases. Essential oils are known for their antibacterial, antifungal, antiviral, insecticidal, and antioxidant properties. This study evaluated the antifungal activity of essential oil from E. kebericho against four common pathogenic fungi and two standard strains. Methods. The essential oil was obtained by hydrodistillation. The antifungal screening was done by agar well diffusion method. Minimal inhibitory concentrations (MICs) were determined by broth microdilution. Minimal fungicidal concentrations (MFCs) were determined by subculturing fungal strains with no visible growth onto a Sabouraud dextrose agar (SDA) plate. Results. Candida albicans and Cryptococcus neoformans were highly sensitive while Aspergillus flavus did not show sensitivity up to 1 mg/ml of essential oil; MICs ranged from 0.083 mg/ml to 0.208 mg/ml. Concentration and fungal species showed significant dose-dependent associations ( p < 0.0001 ) with antifungal activity. The MICs of essential oil were comparable to those of the standard drug (fluconazole) against C. glabrata and C. krusei. The lowest MFC of the essential oil was observed against Candida parapsilosis (0.145 mg/ml) while the highest MFC was against Candida krusei (0.667 mg/ml). Conclusion. Echinops kebericho essential oil showed noteworthy antifungal activity against Cryptococcus neoformans, Candida albicans, and Candida glabrata and could be a potential candidate for further antifungal drug development.

scholarly journals The inhibition activity of essential oil and crude extract of nutmeg seed’s (Myristica fragrans Houtt and Myristica fattua Houtt) on the growth of Xanthomonas campestris Oammel from broccoli (Brassica oleracea var. italica)

2003 ◽  
Vol 1 (1) ◽  
pp. 20-24 ◽  
Author(s):  
GALUH SARI KUSUMANINGRUM ◽  
SURANTO SURANTO ◽  
RATNA SETYANINGSIH
Keyword(s):  
Essential Oil ◽  
Brassica Oleracea ◽  
Crude Extract ◽  
Xanthomonas Campestris ◽  
Inhibition Zone ◽  
Diffusion Method ◽  
Inhibition Activity ◽  
Disk Diffusion Method ◽  
Myristica Fragrans ◽  
Paper Disk

The aims of this research were to study the inhibition activity of essential oil and crude extract of nutmeg seed (Myristica fragrans and M. fattua) on growth of Xanthomonas campestris which is pathogen to broccoli (Brassica oleracea var. italica), to find out the best concentration of those substances to prevent X. campestris and to compare the inhibition activity of both substances on growth of X. campestris. The essential oil of nutmeg seed (M. fragrans and M. fattua) was obtained by Stahl distillation, while crude extract of the nutmeg seeds was extracted by methanol method. The method of this research was the disk diffusion method on nutrient agar. Paper disk was prestreaked on agar medium with X. campestris to be tested. Each petri disk was placed 5 paper disk dropped with the essential oil and the crude extract of the nutmeg seed in concentrations of 100%, 10%, 1% respectively, while for the comparison, the bactericide Agrept 0,2% was used. The parameters measured were the inhibition zone from the essential oil and the crude extract of the nutmeg seeds. The result of the analysis indicated that the essential oil of M. fragrans effectively inhibited X. campestris starting at concentration of 1%, the essential oil of M. fattua effectively inhibited X. campestris at concentration of 10% and the crude extract of M. fragrans effectively inhibited X. campestris at concentration of 100%. The crude extract of M. fattua was not effective in inhibiting the growth of X. campestris.

scholarly journals Uji Daya Hambat Isolat Actinomycetes sebagai Antibakteri terhadap Pertumbuhan Pseudomonas aeruginosa ATCC 27853 secara In Vitro

2021 ◽  
Vol 10 (1) ◽  
pp. 16
Author(s):  
Saskia Arientika Wahyuningrum ◽  
Meiskha Bahar ◽  
Andri Pramesyanti Pramono
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Inhibition Zone ◽  
Diffusion Method ◽  
P Value ◽  
Gram Positive Bacteria ◽  
Mueller Hinton ◽  
Mueller Hinton Agar ◽  
The One ◽  
One Way Anova

Pneumonia is a lung parenchymal infection caused by Pseudomonas aeruginosa.It is Gram negative bacteria that have developed antibiotic resistance. Actinomycetes are Gram-positive bacteria that produce secondary metabolites which have the ability as antimicrobial. Objectives: To identified the ability of Actinomycetes isolates to inhibit the growth of the bacterium Pseudomonas aeruginosa ATCC 27853. The samples in this experiment were from Kebun Raya Bogor that had been rejuvenated on Starch Casein Agar (SCA). Methods: Six dilution series 10-1; 10-2; 10-3; 10-4; 10-5; 10-6 Actinomycetes isolates were used to observe the inhibition zone of P.aeruginosa growth on Mueller Hinton Agar (MHA) media by diffusion method. Results: The effective incubation time occurred at 24 hours, and then it resulted in the average clear zone diameter of 14.70 mm, 10.57 mm, 8.53 mm, 8.47 mm, 6.97 mm, and 5.30 mm. The results of the One – Way Anova test with p-value = 0.000 (p < 0.005) showed some differences at each concentration to inhibit the growth of P.aeruginosa ATCC 27853 at 24 hours incubation period. Conclusion: The most effective concentration of Actinomycetes isolates that can potentially be antibacterial was the concentration of 10-1 with potential solid inhibitory power.Keywords: Actinomycetes, antibacterial, Pseudomonas aeruginosa

Antimicrobial and Antiochratoxic Activities of Mentha spicata Essential Oil

2021 ◽  
Author(s):  
M. Brahmi ◽  
D.E.H. Adli ◽  
M.Y. Boufadi ◽  
W. Arabi ◽  
K. Kahloula ◽  
...  
Keyword(s):  
Candida Albicans ◽  
Essential Oil ◽  
High Performance ◽  
Pathogenic Bacteria ◽  
Production Capacity ◽  
Aspergillus Ochraceus ◽  
Diffusion Method ◽  
Bacterial Strains ◽  
Mentha Spicata

The principle aim of this work is to evaluate the antimicrobial and antiochratoxic power of the essential oil of Mentha spicata (EOM). This oil was obtained by hydrodistillation with a yield of 0.3%. EOM was screened for its possible antibacterial activity in vitro against five strains of pathogenic bacteria, using the solid disc diffusion method and the microdilution method. The extract reacted positively to the bacterial strains tested. The results of the antifungal activity show an inhibition of mycelial growth; it is total on Aspergillus ochraceus, Aspergillus flavus, Aspergillus niger, Penicillium viridicatum, and on Rhizopus stolonifer and Alternaria alternate at a concentration of 12.5 μl/ml. Tests carried out on yeasts mainly referenced as Candida albicans IP444 and Candida albicans 2679 showed minimal inhibition (MIC) of the order of 7.81 μl/ml and 0.97 μl/ml, respectively and theMICis of the order of 0.48 μl/ml for Candida albicans 10 231. In addition, the EOM antiochratoxigenic test for ochratoxin A production capacity following a series of high performance liquid chromatography separation and ultraviolet detection (HPLC–UV) analyses detected an inhibitory effect proportional to the EOM concentration. In general, the essential oil showed an effective antimicrobial action on the growth of the tested pathogens and an antiochratoxigenic.

scholarly journals PERBEDAAN ZONA HAMBAT PERTUMBUHAN Staphylococcus aureus PADA BERBAGAI KONSENTRASI REBUSAN DAUN SALAM (Syzygium polyanthum) SECARA IN VITRO

2018 ◽  
Vol 5 (2) ◽  
Author(s):  
Nyoman Mastra
Keyword(s):  
Staphylococcus Aureus ◽  
Growth Inhibition ◽  
Average Diameter ◽  
Inhibition Zone ◽  
Negative Control ◽  
Diffusion Method ◽  
Disk Diffusion Method ◽  
Growth Inhibition Zone ◽  
Significant Difference

AbstractBackground Staphylococcus aureus is one of causes infection and this bacteria have been resistence for many antibiotic. Bay leaf have antibacterials substance, which stew leaves can be treat infection caused Staphylococcus aureus. The purpose of this study was to determine differences in growth inhibition zone of Staphylococcus aureus at various concentrations of water stew of bay leaf.Method The method of this study is true experiment with posstest only control design, and used Kirby Bauer disk diffusion method with various concentrations of water stewed of bay leaf (20%, 40%, 60%, 80%,100%), positive control (chloramfenicol 30 μg) and negative control (sterile distilled water). Result The result showed that the average diameter of inhibition zone in concentration 20%, 40%, 60%, 80%, and 100% is 7 mm, 8,4 mm, 9,6 mm, 10,5 mm and 11,5 mm. Based on statistical analysis using oneway ANOVA available the value of p (0,000) α (0,05), so the inhibition zone is significant difference of growth inhibition zone of Staphylococcus aureus at various concentrations of stewed water bay leaf. Conclusion Water stew of bay leaf can inhibit the growth of Staphylococcus aureus, and there are differences in growth inhibition zone of Staphylococcus aureus at various concentrations of water stew of bay leaf. Keywords: stew of bay leaf; Staphylococcus aureus; inhibition zone 

scholarly journals In vitro Inhibitory Activity of Ethanolic Fruit Extract from Averrhoa bilimbi L. against Streptococcus pyogenes Bacteria

2019 ◽  
Vol 3 (1) ◽  
pp. 13-17
Author(s):  
Puspita Dewi ◽  
Gusti Ayu Ratih ◽  
Burhannuddin Burhannuddin ◽  
Gede Sudarmanto
Keyword(s):  
Streptococcus Pyogenes ◽  
Inhibitory Activity ◽  
Pathogenic Bacteria ◽  
Respiratory Infections ◽  
Inhibition Zone ◽  
Negative Control ◽  
Diffusion Method ◽  
Fruit Extract ◽  
Significant Difference

Averrhoa bilimbi L., popularly known as belimbing wuluh, is a common plant used as traditional medicine and cooking ingredients in Indonesia. Averrhoa bilimbi fruit contains many active substances as antibacterial such as tannin, formic acid, flavonoids, saponins, peroxides, and triterpenoids. Streptococcus pyogenes is a pathogenic bacteria that can cause respiratory infections. This study was carried out to investigate the inhibitory activity of Averrhoa bilimbi L. fruit extract at various concentrations on the growth of Streptococcus pyogenes. This study was a true experimental with post test only control design. The inhibitory activity was assessed by the KirbyBauer’s disc diffusion method with four different extract concentrations (20%, 40%, 60%, 80%), 96% EtOH as negative control, and Amoxicillin antibiotic as positif control. The EtOH extract showed the mean diameter of inhibition zone of 20% extract was 11.43 mm (strong), 40% extract was 19.13 mm (strong), 60% extract was 24.58 mm (very strong), 80% extract was 30.99 mm (very strong), and the negative control (96% EtOH) was 0.00 mm. Least Significant Difference test showed there were significant different of inhibition zone in each extract concentrations of Averrhoa bilimbi L. against Streptococcus pyogenes with the strongest inhibitory activity is 80% extract concentration. Keywords: Averrhoa bilimbi, Inhibitory activity, Streptococcus pyogenes

scholarly journals The Difference of Antibacterial Power between Cocoa Peel (Theobroma Cacao L.) Extract 6% compared to Chlorhexidine Digluconate 2% Againts Streptococcus mutans (In vitro)

2021 ◽  
Vol 11 (1) ◽  
pp. 1
Author(s):  
Alissa Amanda ◽  
Tamara Yuanita ◽  
Galih Sampoerno
Keyword(s):  
Streptococcus Mutans ◽  
Theobroma Cacao ◽  
Inhibition Zone ◽  
Diffusion Method ◽  
Chlorhexidine Digluconate ◽  
Significant Difference ◽  
The Difference ◽  
Theobroma Cacao L ◽  
Peel Extract

Background: Before restoration, it is necessary to clean the cavity from the smear layer and residual bacteria such as Sreptococcus mutans using a 'gold standard' cavity cleanser, namely 2% Chlorhexidine digluconate (CHX), however CHX 2% has a disadvantage of having a toxic effect on fibroblasts, osteoblasts, myoblasts, odontoblast-like cells, Chinese hamster ovary cells, and buccal epithelial cells. The shortcomings of the 2% CHX triggered researchers to look for alternative cavity cleansers that are more biocompatible, namely cocoa peel extract because it contains of antibacterial compounds including alkaloids, flavonoids, tannins, saponins, and terponoids with a non-toxic 6% concentration. Purpose: To analyze the difference of antibacterial activity between cocoa peel extract with a concentration of 6% compared to chlorhexidine digluconate 2% against Streptococcus mutans. Methods: This research was an in vitro laboratory experimental study with the posttest only control group design which included two treatment groups, namely 6% cocoa peel extract and 2% CHX. This research was conducted using the inhibition zone diffusion method against S. mutans to see the antibacterial power of each sample. Results: There was a significant difference (p <0.05) in the mean diameter of the inhibition zone between 6% cacao peel extract, namely 11.5406 mm and CHX 2%, namely 13.2156 mm.  Conclusion: Chlorhexidine digluconate 2% has a greater antibacterial power than 6% cocoa peel extract (Theobroma cacao L.) against Streptococcus mutans. 

scholarly journals Evaluation of Anti Microbial and Anti Fungal Activity of Acalyphaindica L., Leaf Extract

2018 ◽  
Vol 10 (01) ◽  
Author(s):  
Sudhakar Chekuri ◽  
Arun Jyoti B ◽  
Saraswathi JSompaga ◽  
Shivaprasadi Panjala ◽  
Roja Ran Anupalli
Keyword(s):  
Staphylococcus Aureus ◽  
Candida Albicans ◽  
Aspergillus Niger ◽  
Ethanolic Extract ◽  
Inhibition Zone ◽  
Diffusion Method ◽  
Fungal Activity ◽  
Bacteria And Fungi ◽  
Anti Fungal ◽  
Acalypha Indica

Anti-microbial and anti-fungal activity of different solvent extracts of Acalypha indica (Euphorbeace family) was tested against bacterial pathogens (Pseudomonasaeruginosa, E.Coli, KlebsiellaPneumonia and Staphylococcus aureus) and fungal strains (Candida albicans, Aspergillus niger, Candida tropicalis andCandida kefyr) using the Agar Well diffusion method . It was observed that all the extracts showed positive activity) against bacteria and fungi. Ethanolic extract of Acalypha indica showed more potency against Staphylococcus aureus with an inhibition zone of12.46 (mm) and Methanolic extract exhibited higher activity against E.coli with an inhibition zone of11.26 (mm). Ethanolic extract of Acalypha indica showed prominent antifungal activity against candida albicans with an inhibition diameter of 12.53 (mm) and Aspergillus niger with a diameter of 9.21 (mm) when compared to other solvent extracts. Erythromycin and Ketoconazole were used as positive standards for antimicrobial and anti fungal experiments. In the present study, Ethanol extract showed a varying degree of inhibition to the growth of tested organisms compared to Methanol, Acetone and Chloroform against Bacteria and Fungi. The results confirmed the presence of antibacterial and antifungal compounds in shade dried extracts of Acalypha indica against human pathogenic organisms.

scholarly journals The Effect of Water-Soluble Stem Extract “Kayu Kuning“ (Arcangelisia flava L.Merr) On The Growth Inhibition of Candida albicans ATCC 10231 and Trichophyton mentagrophytes IN VITRO

2014 ◽  
Vol 3 (1) ◽  
pp. 15
Author(s):  
Rini Setyowati ◽  
Sudarsono Sudarsono ◽  
Setyowati E P
Keyword(s):  
Candida Albicans ◽  
Trichophyton Mentagrophytes ◽  
Inhibition Zone ◽  
Negative Control Group ◽  
Negative Control ◽  
Test Solution ◽  
Water Soluble ◽  
Control Group ◽  
One Way Anova

<p>“Kayu kuning” (<em>Arcangelisia flava </em>L.Merr) was used when someone has a skin problem caused by <em>Candida </em>albicans<em> </em>and <em>Trichophyton mentagrophytes</em>. Scientific based medicine on this traditional knowledge was necessary be  done. Stem powderwas extracted by  distilled water.The extract was then evaporated. Qualitative and quantitative analysis of the active substance e.g., Berberin chloride by Thin Layer Chromatography (TLC)  The antifungal activity againts <em>Candida albicans</em> and <em>Trichophyton mentagrophytes</em>were tested by using agar diffusion and microdilution methods.  The absorbance from microdilution were analized by One way ANOVA. The conclusion showed that the extract contained 1.55±0.12% w/walkaloid calculated as Berberine chloride. The inhibition zone for <em>Candida albicans </em>and <em>Trichophyton mentagrophytes </em>were 16.65±4.52 and 6.55±0.05 mm respectively. The MIC vallue for both fungi was 10 mg/mL.The MBC value for <em>Candida albicans</em> was 40 mg/mL and for <em>Trichophyton mentagrophytes </em>was 50 mg/mL. From the analysis with one-way ANOVA, shows that there are significant differences between the positive control group and the test solution with the negative control group with p=0.020 for <em>Candida albicans</em> and p=0.028 for <em>Trichophyton mentagrophytes</em> (p&lt;0.050). Post hoc Tukey analysis results showed  that both inter-group and between the concentration of the test solution to the control group did not differ significantly positive because the value of p&gt;0.050.</p>

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