scholarly journals Biosynthesis and Characterization of Silver Nanoparticles Produced by Plant Extracts and Its Antimicrobial Activity

2019 ◽  
pp. 1-14
Author(s):  
Marwa A. Samy ◽  
Moustafa A. Abbassy ◽  
Elsayed E. Hafez ◽  
Entsar I. Rabea ◽  
Dalia G. Aseel
Keyword(s):  
Silver Nanoparticles ◽  
Brown Rot ◽  
Wild Plants ◽  
Diffusion Method ◽  
Dry Rot ◽  
Bacterial Diseases ◽  
Physalis Peruviana ◽  
Growth Method ◽  
Rot Disease

Solanum tubersum is the fourth most challenging plant in Egypt, affected by several fungi, viral and bacterial diseases. Bacterial and fungal isolates (Brown rot disease (Ralstonia solaniserum), soft root disease (Pectobacterium carotovora) and dry rot disease (Fusarium oxisporum) were collected. The green extracts of silver nanoparticles were prepared by means of aqueous extracts of three wild plants, Physalis peruviana (leaves, red and green fruits) (N1, N2 and N3), Solanum nigrum (fruit) (N4) and Moringa oliefera (leaves) (N5). SEM, TEM, FT-IR and X-RD obtained the characterization of the biosynthesis of silver nanoparticles. The results indicated that nanoparticles were spherical, smooth and the sizes varied between 12 and 33 nm. The activity of the nanoparticle formulations was tested against the bacterial isolates using agar diffusion method and one fungus using mycelial growth method. The results also elucidated that N5 formulation showed a significantly potent antibacterial activity against R. solanacearum. However, N1 formulation was the highest active one against P. carotovra. In addition, the antifungal activity indicated that N1 had the highest effect (EC50 = 687.03 mg/L) followed by N3 (EC50 = 981.61 mg/L) against F. oxysporium. Nanoparticles synthesized by wild plants could be used as safe alternatives to harmful microbicides.

scholarly journals Identification and Characterization of Three Monilinia Species from Plum in China

Plant Disease ◽  
2015 ◽  
Vol 99 (12) ◽  
pp. 1775-1783 ◽  
Author(s):  
Liang-Fen Yin ◽  
Shu-Ning Chen ◽  
Guo-Kang Chen ◽  
Guido Schnabel ◽  
Sheng-Fang Du ◽  
...  
Keyword(s):  
Brown Rot ◽  
Single Spore ◽  
Chongqing Municipality ◽  
Prunus Salicina ◽  
Size Number ◽  
Colony Color ◽  
Rot Disease ◽  
Germ Tubes ◽  
Colony Shape

In total, 112 Monilinia spp. single-spore isolates were collected from plum fruit (Prunus salicina) symptomatic for brown rot disease from Yunnan, Hubei, and Zhejiang provinces and Chongqing municipality, China between 2012 and 2014. Three distinct colony morphologies (phenotypes) were observed on potato dextrose agar and two isolates per phenotype were selected for further analysis. Colony color, colony shape, conidia size, number of germ tubes per conidia, and pathogenicity on plum were investigated. The ribosomal internal transcribed spacer regions 1 and 2 as well as a polymerase chain reaction-based method that amplified fragments of the glyceraldehyde-3-phosphate dehydrogenase (G3PDH) and β-tubulin (TUB2) genes were used to identify the isolates to the species level. The three phenotypes were identified to be three different species: Monilinia fructicola, Monilia mumecola, and Monilia yunnanensis. Phylogenetic analysis based on G3PDH and TUB2 nucleotide sequences revealed that isolates within species clustered together regardless of host or geographical origin, suggesting that these factors did not play an important role for the evolutionary separation of the described species.

scholarly journals Terrestrial snail-mucus mediated green synthesis of silver nanoparticles and in vitro investigations on their antimicrobial and anticancer activities

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Pramod C. Mane ◽  
Shabnam A. R. Sayyed ◽  
Deepali D. Kadam ◽  
Manish D.Shinde ◽  
Amanullah Fatehmulla ◽  
...  
Keyword(s):  
Silver Nanoparticles ◽  
Anticancer Activity ◽  
Diffusion Method ◽  
Anticancer Activities ◽  
The Past ◽  
Interesting Possibility ◽  
Uv Visible ◽  
Topical Applications

AbstractOver the past few years, biogenic methods for designing silver nanocomposites are in limelight due to their ability to generate semi-healthcare and para-pharmaceutical consumer goods. The present study reports the eco-friendly synthesis of silver nanoparticles from the hitherto unexplored mucus of territorial snail Achatina fulica by the facile, clean and easily scalable method. The detailed characterization of the resultant samples by UV–Visible Spectroscopy, FESEM-EDS, XRD and FTIR Spectroscopy techniques corroborated the formation of silver nanoparticles in snail mucus matrix. The resultant samples were tested against a broad range of Gram positive and Gram negative bacteria like Escherichia coli, Staphylococcus aureus, Klebsiella pneumoniae, Pseudomonas aeruginosa and a fungal strain Aspergillus fumigatus by well diffusion method. The results indicate that silver nanoparticles in mucus matrix exhibit strong antibacterial as well as antifungal activity. The pertinent experiments were also performed to determine the inhibitory concentration against both bacterial and fungal strains. Anticancer activity was executed by in vitro method using cervical cancer cell lines. Curiously, our biogenically synthesized Ag nanoparticles in biocompatible mucus revealed anticancer activity and demonstrated more than 15% inhibition of Hela cells. We suggest an interesting possibility of formulating antimicrobial and possibly anticancer creams/gels for topical applications in skin ailments.

scholarly journals Synthesis and characterization of chitosan nanoparticles of Achillea millefolium L. and their activities

F1000Research ◽  
2020 ◽  
Vol 9 ◽  
pp. 1297
Author(s):  
Dolly Kain ◽  
Suresh Kumar
Keyword(s):  
Sodium Tripolyphosphate ◽  
Chitosan Nanoparticles ◽  
Diffusion Method ◽  
Achillea Millefolium ◽  
Aggregation Assay ◽  
Jammu And Kashmir ◽  
Bacterial Diseases ◽  
Antibacterial Screening ◽  
Vis Spectroscopy

Background: Achillea millefolium L. is an herbal aromatic plant of family Asteraceae reported to have various medicinal activities in the literature. The current study evaluated the potential of chitosan nanoparticles of A. millefolium as an effective strategy for targeted treatment of bacterial diseases and urolithiasis. Methods: A. millefolium was collected from Poonch, Jammu and Kashmir, and its inflorescence extracted in water by maceration. Chitosan nanoparticles of A. millefolium (AMCSNPs) were prepared by ionic gelation method using 0.1% chitosan, different concentrations of the cross-linking agent sodium tripolyphosphate (STPP; 0.5%, 1%, 1.5%, 2%) and different concentrations of A. millefolium extract (0.5%, 1%, 1.5%, 2%). Characterization of AMCSNPs was done using UV-Vis spectroscopy, Fourier transform-infrared (FT-IR) spectroscopy, dynamic light scattering (DLS) and transmission electron microscopy (TEM). Antibacterial screening of AMCSNPs was performed by well-diffusion method. Antiurolithiatic screening of AMCSNPs was done by nucleation and aggregation assay. Results: The best chitosan nanoparticles of A. millefolium (AMCSNPs) were obtained with 0.1% chitosan, 1% STPP and 20% A. millefolium. These AMCSNPs showed maximum zone of inhibition of 30±0.5 mm using the well-diffusion method against both Bacillus subtilis (Gram-positive) and Pseudomonas aeruginosa (Gram-negative) and maximum antiurolithiatic activity with 68% inhibition shown at aggregation stage. Conclusions: The current study suggests that AMCSNPs are an excellent strategy for targeted drug delivery for treatment of bacterial diseases and urolithiasis.

scholarly journals Synthesis, Characterization and Anti-Microbial Activity of Citrus limon Mediated Nanoparticles

2018 ◽  
Author(s):  
Fouzia Gul Samreen ◽  
Rabeea Muzaffar ◽  
Muhammad Nawaz ◽  
Shahla Gul ◽  
Muhammad Asim Raza Basra
Keyword(s):  
Silver Nanoparticles ◽  
Average Particle Size ◽  
Antimicrobial Activities ◽  
Diffusion Method ◽  
Citrus Limon ◽  
Average Particle ◽  
X Ray Diffraction ◽  
Cost Efficient ◽  
Peel Extract

Previously the nanoparticles were synthesized by chemical methods which were costly and toxic to bio-systems. Plant extracts provides simpler, eco-friendly and cost efficient method for synthesizing nanoparticles. Lemon peel extract (LPE) was used to synthesize silver nanoparticles (AgNPs) which were evaluated for their antimicrobial effects after optimizing the pH of extract and concentration of both extract and synthesized AgNPs. The characterization of synthesized AgNPs was carried out using Ultraviolet-Visible (UV-Vis) Spectrophotometer, Fourier transform infrared spectroscopy (FTIR), and X-ray diffraction (XRD). Well diffusion method was used to determine the antimicrobial activities of synthesized AgNPs. The presence of phenols and proteins was assumed to reduce the Ag+ ion into silver nanoparticles. The characteristic surface plasmon resonance frequency was observed at 405–425 nm for all varying condition of silver nanoparticles synthesis. Furthermore, results revealed that the synthesized AgNPs remains stable upto 75 days. The average particle size was 2–5 nm, calculated with the help of scherrer’s equation by using XRD data. LPE mediated AgNPs (200 µg/mL) showed significant antimicrobial activity, compared to commercially available nanoparticles while LPE (50 mg/ml) showed no effect. LPE mediated AgNPs might get attention of pharmacists in order to design medicines against different diseases including the infections of bacteria.

scholarly journals Prevalence and Characterization of Streptococcus pyogenes Clinical Isolates from Different Hospitals and Clinics in Mansoura

2020 ◽  
Vol 2020 ◽  
pp. 1-11
Author(s):  
Zeinab M. Helal ◽  
Dina E. Rizk ◽  
Mohamed M. Adel El-Sokkary ◽  
Ramadan Hassan
Keyword(s):  
Virulence Factors ◽  
Streptococcus Pyogenes ◽  
Molecular Typing ◽  
Resistance Mechanisms ◽  
Latex Agglutination ◽  
Diffusion Method ◽  
Resistance Testing ◽  
Bacterial Diseases ◽  
Amoxicillin Clavulanic Acid

Streptococcus pyogenes are associated with many bacterial diseases in both humans and animals and are capable of causing a multitude of human diseases. S. pyogenes isolates were identified by their bacitracin sensitivity, positive spy1258 detection, and positive GAS latex agglutination. Different isolates were typed serotypically and genotypically by BOX-PCR. Different virulence factors were identified in S. pyogenes isolates. In addition, antimicrobial resistance was tested to eleven different antibiotics. Furthermore, the resistance mechanisms were determined phenotypically by the disc diffusion method. Finally, the correlation between both molecular and serotypes identified and the profile of virulence factors and clinical and geographical sources was determined for all isolates. Thirty-eight S. pyogenes isolates were collected from different clinical sources. Resistance testing indicated high resistance to mostly used antibiotics except amoxicillin/clavulanic acid, amoxicillin, and ampicillin. Serotyping results indicated five different serotypes, M1, M2, M3, M4, and M6, in S. pyogenes isolates, while six isolates were identified as untypeable. In addition, positive PCR results identified most of the tested SAgs genes in which speJ gene was mostly identified followed by speI, speC, and ssa genes being identified in 81.6%, 63.3%, 60.5%, and 60.5%, respectively. However, speH was the least detected. In contrast, speL, speM, and smeZ genes could not be detected in all tested isolates. Finally, BOX-PCR molecular typing was a more effective clustering method when compared to the serotyping method in all S. pyogenes. In conclusion, the isolates in this study were highly resistant to mostly used antibiotics. M1 was the most identified serotype. No significant association was found between serotypes, BOX-PCR cluster groups, and SAgs genes profiles. However, by the application of BOX-PCR, effective molecular typing was obtained.

scholarly journals Characterization and Antibacterial Activity of Silver Nanoparticles Biosynthesized Using Leaves Extract of Artemisia sieberi and Calotropis procera

2021 ◽  
Vol 72 (2) ◽  
pp. 76-82
Author(s):  
Saleh H. Salmen ◽  
Nadiah Matluq Alkammash ◽  
Tahani Awad Alahmadi ◽  
Sulaiman Ali Alharbi
Keyword(s):  
Silver Nanoparticles ◽  
Antibacterial Activity ◽  
Diffusion Method ◽  
Calotropis Procera ◽  
Resistant Bacteria ◽  
Antibiotic Resistant ◽  
Transmission Electron ◽  
Average Size

The prevalence of antibiotic-resistant bacteria has increased recently leading to the need for novel, natural antibacterial agents such as plant-synthesized silver nanoparticles. Such synthesis is safe, cheap, rapid, non-toxic and environmentally friendly. In this study, characterization of biosynthesized silver nanoparticles from extracts of A. sieberi and C. procera was carried out using transmission electron microscopy, fourier transform infrared and energy dispersive x-ray analysis. Spherical nanoparticles with an average size was ~10 nm for A. sieberi and ~14 nm for C. procera were synthesised; synthesis was most effective using A. sieberi. Antibacterial activity of silver nanoparticles was carried out using the agar-diffusion method and by determination of the minimum inhibitory concentration. Biosynthesized silver nanoparticles showed antibacterial activity against Staphylococcus aureus, MRSA, Salmonella typhimurium and Escherichia coli, with silver nanoparticles extracts from A. sieberi being the most antibacterial.

Synthesis and Characterization of Silver Nanoplates by a Seed-mediated Method

2019 ◽  
Vol 29 (3) ◽  
Author(s):  
Mai Ngọc Tuan Anh
Keyword(s):  
Hydrogen Peroxide ◽  
Silver Nanoparticles ◽  
Ascorbic Acid ◽  
Silver Nitrate ◽  
Sodium Borohydride ◽  
Trisodium Citrate ◽  
Synthesis And Characterization ◽  
Silver Nanoplates ◽  
Seed Mediated

Silver nanoplates (SNPs) having different size were synthesized by a seed-mediated method. The seeds -silver nanoparticles with 4 – 6 nm diameters were synthesized first by reducing silver nitrate with sodium borohydride in the present of Trisodium Citrate and Hydrogen peroxide. Then these seeds were developed by continue reducing Ag\(^+\) ions with various amount of L-Ascorbic acid to form SNPs. Our analysis showed that the concentratrion of L-Ascorbic acid, a secondary reducing agent, played an important role to form SNPs. In addition, the size and in-plane dipole plasmon resonance wavelenght of silver nanoplates were increased when the concentration of added silver nitrate increased. The characterization of SNPs were studied by UV-Vis, FE-SEM, EDS and TEM methods.

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