Antimicrobial Resistance Profile of Escherichia coli Isolates from Different Clinical Samples in Abeokuta, Ogun State

The alarming increase of antibiotic resistance of Escherichia coli has posed a great challenge in the public health sector. Thus, this microorganism is a leading cause of different human infections and it can be found in various environments. The aim of this study is to investigate the antimicrobial susceptibility patterns and the multiple antimicrobial resistance profile of Escherichia coli isolates obtained from some hospitals in Abeokuta, Ogun State, Nigeria. Isolates of E. coli were obtained from different clinical samples and were re-identified morphologically and biochemically. E. coli was isolated from 30% out of a total of 70 clinical samples analyzed for isolation and identification. The isolation rate of E. coli was highest in urine samples 10(47.6%) when compared to other clinical samples. There was significant increase in the resistance rate of E. coli to tetracycline (14.3%), ceftazidime (14.2%), and ampicillin (14.2%).Also, an increased sensitivity rate to augmentin (71.4%), ofloxacin (66.7%), cefuroxime (66.7%), ciprofloxacin (61.9%) and ceftazidime (61.9%) were observed. Furthermore, the overall multiple drug resistance rates obtained was 14(66.7%) and it was established that, multiple antimicrobial resistance of the E. coli isolates was plasmid mediated. E. coli isolates exhibited high resistance rate to multiple antimicrobial agents, however, its sensitivity to augmentin, ofloxacin, cefuroxime, ciprofloxacin and ceftazidime showed that these antimicrobials are still effective against E. coli infections in the study area.

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Antimicrobial resistance profile of extended- spectrum Beta-Lactamase producing Escherichia Coli at Ho Chi Minh City

Science and Technology Development Journal - Natural Sciences ◽

10.32508/stdjns.v4i1.910 ◽

2020 ◽

Vol 4 (4) ◽

pp. first

Author(s):

Ngan Ly Hoang Nguyen ◽

Trang Thi Phuong Phan ◽

Nguyen Kim Thi Quyen

Keyword(s):

Antimicrobial Resistance ◽

High Resistance ◽

Multidrug Resistant ◽

Resistance Rate ◽

Ho Chi Minh City ◽

Healthy Individuals ◽

Resistance Profile ◽

Extended Spectrum Beta Lactamase ◽

E Coli ◽

Antimicrobial Resistance Profile

The antimicrobial resistance profile of extended-spectrumb -lactamase (ESBL) producing E. coli has been continuously changed in recent years in Vietnam and wordwide. A number of studies recently found that the prevalences of ESBL-producing E. coli decreased in Vietnam from 2016 to 2017 compared with those in previous years. The purpose of this study was to determine the prevalence and antimicrobial profile of ESBL-producing E. coli isolated from clinical specimens in Binh Dan hospital and from healthy individuals at Ho Chi Minh City in 2018. The results showed that the prevalence of ESBL-producing E. coli isolated from healthy individuals in 2018 and 2017 were nearly equal. The prevalence increased from 14.9% in 2017 up to 19.1% in 2018. The prevalence of ESBL-producing E. coli isolated from healthy individuals in Ho Chi Minh City was lower than that in other parts of Viet Nam. The prevalence of ESBL-producing E. coli isolated from clinical pecimens in Binh Dan hospital (22.25%) was lower than those in some other hospitals but higher compared with it from healthy individuals (19.1%). All of the ESBL-producing isolates were multidrug resistant and high resistance to aminoglycoside or quinolone. Imipenem and fosfomycin have still be considered as the treatment of choice against ESBL- producer infections. High resistance rate of E. coli isolated from blood specimens to imipenem was found in the study.

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Antimicrobial Resistance Profile of Escherichia coli Isolated from Urine of Patients in Selected General Hospitals in Abuja Municipal, Nigeria

Asian Journal of Advanced Research and Reports ◽

10.9734/ajarr/2018/v2i229745 ◽

2018 ◽

pp. 1-10

Author(s):

M. O. Eghieye ◽

S. M. Jodi ◽

B. E. Bassey ◽

I. H. Nkene ◽

R. H. Abimiku ◽

...

Keyword(s):

Escherichia Coli ◽

Antibiotic Resistance ◽

Antimicrobial Resistance ◽

Clavulanic Acid ◽

General Hospital ◽

General Hospitals ◽

Resistance Profile ◽

E Coli ◽

Antimicrobial Resistance Profile ◽

Amoxicillin Clavulanic Acid

This study investigated the antimicrobial resistance profile of Escherichia coli from urine of patients with suspected urinary tract infections (UTIs) in selected general hospitals in Abuja Municipal, Nigeria. Four Hundred and Thirty urine samples were collected between September 2017 and May 2018 from patients attending Asokoro General Hospital (AGH), Garki Hospital Abuja (GHA) and Wuse General Hospital (WGH); and E. coli was isolated and identified by culture, microscopy and biochemical tests. The overall occurrence of E. coli was 52 (12.1%). The occurrences in relation to the hospitals were of the order: GHA (14.7%) > WGH (12.6%) > AGH (9.0%). The highest (50%) occurrence was at age 41-50 years in WGH, and the lowest (4.3%) was at age 31-40 years in AGH. More females than males harboured the bacteria in all the hospitals. Isolates from AGH showed highest (100.0%) resistance to Sulphamethoxazole/Trimethoprim but least (0.0%) resistance to Ciprofloxacin. Isolates from GHA showed the highest resistance to Cefotaxime and Streptomycin (95.2%) but least (23.8%) to Gentamicin and Imipenem. Isolates from WGH showed highest (88.8%) resistance to Amoxicillin/Clavulanic Acid but least (16.7%) to Sulphamethoxazole/Trimethoprim. The commonest antibiotic resistance phenotype in AGH was Amoxicillin/Clavulanic Acid-Streptomycin-Cefotaxime-Ceftazidime-Imipenem-Ampicillin (3.9%); in GHA was Amoxicillin/Clavulanic Acid-Streptomycin-Sulphamethoxazole/Trimethoprim-Cefotaxime-Ceftazidime-Ampicillin (7.7%); and in WGH was Amoxicillin/Clavulanic Acid-Streptomycin-Cefotaxime-Cefotaxime-Ceftazidime-Imipenem-Ampicillin (3.9%). All the isolates had MAR indices above 0.2; the most common index in AGH was 0.4 (at 30.8%), GHA was 0.7 (at 33.3%) and WGH was 0.7 (at 27.8%). The commonest class of antibiotic resistance was MDR with the order of occurrence as: GHA (92.2%) > WGH (77.7%) > AGH (76.6%). Ciprofloxacin, gentamicin and imipenem were the most effective antibiotics in the study location. However, MAR indices in this study have shown that the isolates originated from an environment where antibiotics are freely available and misused/abused. Hence, there is a need for greater monitoring of antibiotic supplies and use.

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Antimicrobial resistance profile of microbial pathogens isolated from hospitals of Colatina-ES in the period of 2008-2010

Revista de Investigação Biomédica ◽

10.24863/rib.v8i1.30 ◽

2016 ◽

Vol 8 (1) ◽

pp. 61

Author(s):

Deuvânia Cabral Azevedo ◽

Marcus Vinícius Dias-Souza ◽

Andrea De Souza Monteiro

Keyword(s):

Escherichia Coli ◽

Staphylococcus Aureus ◽

Pseudomonas Aeruginosa ◽

Antimicrobial Resistance ◽

Microbial Pathogens ◽

Resistance Profile ◽

E Coli ◽

Antimicrobial Resistance Profile ◽

Santa Maria

A resistência bacteriana é um mecanismo usado pelas bactérias para interferir na ação dos antibióticos e vem crescendo consideravelmente, se tornando um grande problema de saúde pública. O presente estudo avaliou o perfil de resistência de cepas de Staphylococcus aureus, Pseudomonas aeruginosa e Escherichia coli e a suscetibilidade das mesmas à alguns antimicrobianos. Para isso realizou-se uma pesquisa quantitativa utilizando os resultados de antibiogramas constantes nos livros de registro microbiologia do laboratório LAC - Santa Maria, situado em Colatina - ES. As amostras foram coletadas rotineiramente de pacientes internados em dois hospitais (Santa Maria e São José) e examinados pelo laboratório. No livro de registro obteve-se um total de 446 isolados microbianos identificados, dos quais 277 cepas foram identificadas como: S. aureus (36,5%), P. aeruginosa (32,5%) e E. coli (31%), os 169 restantes englobam os outros micro-organismos. Quanto ao perfil de resistência e sensibilidade, as cepas de P. aeruginosa foram mais sensíveis ao antibiótico sulbactam, E. Coli à ampicilina e ácido nalidíxico, e Staphylococcus à amicacina e ceftriaxone. Relaciona-se o maior isolamento de Stapylococcus, P. aeruginosa e E. coli provavelmente por serem micro-organismos encontrados colonizando ambientes hospitalares e a comunidade com grande potencial de disseminação. Quanto ao perfil de resistência se torna útil na seleção de drogas especificas para determinados micro-organismo. Palavras-chave: Resistência. Antibióticos. Micro-organismos.

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Detection of virulence factors and antimicrobial resistance patterns in shiga toxin-producing Escherichia coli isolates from sheep

Pesquisa Veterinária Brasileira ◽

10.1590/s0100-736x2015000900002 ◽

2015 ◽

Vol 35 (9) ◽

pp. 775-780 ◽

Cited By ~ 5

Author(s):

Marcos R.A. Ferreira ◽

Talícia dos S. Silva ◽

Ariel E. Stella ◽

Fabricio R. Conceição ◽

Edésio F. dos Reis ◽

...

Keyword(s):

Escherichia Coli ◽

Antimicrobial Resistance ◽

Virulence Factors ◽

Shiga Toxin ◽

Resistance Profile ◽

Antimicrobial Resistance Profile ◽

Healthy Sheep ◽

Resistance Patterns ◽

Group A ◽

Stx1 Gene

Abstract: In order to detect virulence factors in Shiga toxin-producing Escherichia coli (STEC) isolates and investigate the antimicrobial resistance profile, rectal swabs were collected from healthy sheep of the races Santa Inês and Dorper. Of the 115 E. coli isolates obtained, 78.3% (90/115) were characterized as STEC, of which 52.2% (47/90) carried stx1 gene, 33.3% (30/90) stx2 and 14.5% (13/90) both genes. In search of virulence factors, 47.7% and 32.2% of the isolates carried the genes saa and cnf1. According to the analysis of the antimicrobial resistance profile, 83.3% (75/90) were resistant to at least one of the antibiotics tested. In phylogenetic classification grouped 24.4% (22/90) in group D (pathogenic), 32.2% (29/90) in group B1 (commensal) and 43.3% (39/90) in group A (commensal). The presence of several virulence factors as well as the high number of multiresistant isolates found in this study support the statement that sheep are potential carriers of pathogens threatening public health.

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Genotyping and antimicrobial resistance in Escherichia coli from pig carcasses

Pesquisa Veterinária Brasileira ◽

10.1590/s0100-736x2017001100010 ◽

2017 ◽

Vol 37 (11) ◽

pp. 1253-1260 ◽

Cited By ~ 1

Author(s):

Caroline Pissetti ◽

Gabriela Orosco Werlang ◽

Jalusa Deon Kich ◽

Marisa Cardoso

Keyword(s):

Escherichia Coli ◽

Antimicrobial Resistance ◽

Antimicrobial Agents ◽

Commensal Bacteria ◽

Pfge Analysis ◽

Gene Detection ◽

E Coli ◽

Antimicrobial Resistance Profile ◽

Resistant Strains ◽

Pig Carcasses

ABSTRACT: The increasing antimicrobial resistance observed worldwide in bacteria isolated from human and animals is a matter of extreme concern and has led to the monitoring of antimicrobial resistance in pathogenic and commensal bacteria. The aim of this study was to evaluate the antimicrobial resistance profile of Escherichia coli isolated from pig carcasses and to assess the occurrence of relevant resistance genes. A total of 319 E. coli isolates were tested for antimicrobial susceptibility against different antimicrobial agents. Moreover, the presence of extended-spectrum β-lactamase (ESBL) and inducible ampC-β-lactamase producers was investigated. Eighteen multi-resistant strains were chosen for resistance gene detection and PFGE characterization. The study showed that resistance to antimicrobials is widespread in E. coli isolated from pig carcasses, since 86.2% of the strains were resistant to at least one antimicrobial and 71.5% displayed multi-resistance profiles. No ampC-producing isolates were detected and only one ESBL-producing E. coli was identified. Genes strA (n=15), floR (n=14), aac(3)IVa (n=13), tetB (n=13), sul2 (n=12), tetA (n=11), aph(3)Ia (n=8) and sul3 (n=5) were detected by PCR. PFGE analysis of these multi-resistant E. coli strains showed less than 80% similarity among them. We conclude that antimicrobial multi-resistant E. coli strains are common on pig carcasses and present highly diverse genotypes and resistance phenotypes and genotypes.

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Antimicrobial Resistance Pattern of Escherichia coli Isolated from Frozen Chicken Meat in Bangladesh

Pathogens ◽

10.3390/pathogens9060420 ◽

2020 ◽

Vol 9 (6) ◽

pp. 420 ◽

Cited By ~ 1

Author(s):

Mst. Sonia Parvin ◽

Sudipta Talukder ◽

Md. Yamin Ali ◽

Emdadul Haque Chowdhury ◽

Md. Tanvir Rahman ◽

...

Keyword(s):

Escherichia Coli ◽

Antimicrobial Resistance ◽

Foodborne Pathogens ◽

Biochemical Properties ◽

Chicken Meat ◽

Diffusion Method ◽

Resistance Pattern ◽

Isolation And Identification ◽

E Coli ◽

Encoding Genes

Escherichia coli is known as one of the most important foodborne pathogens in humans, and contaminated chicken meat is an important source of foodborne infection with this bacterium. The occurrence of extended-spectrum β-lactamase (ESBL)-producing E. coli (ESBL-Ec), in particular, in chicken meat is considered a global health problem. This study aimed to determine the magnitude of E. coli, with special emphasis on ESBL-Ec, along with their phenotypic antimicrobial resistance pattern in frozen chicken meat. The study also focused on the determination of ESBL-encoding genes in E. coli. A total of 113 frozen chicken meat samples were purchased from 40 outlets of nine branded supershops in five megacities in Bangladesh. Isolation and identification of E. coli were done based on cultural and biochemical properties, as well as PCR assay. The resistance pattern was determined by the disc diffusion method. ESBL-encoding genes were determined by multiplex PCR. The results showed that 76.1% of samples were positive for E. coli, of which 86% were ESBL producers. All the isolates were multidrug-resistant (MDR). Resistance to 9–11 and 12–13 antimicrobial classes was observed in 38.4% and 17.4% isolates, respectively, while only 11.6% were resistant to 3–5 classes. Possible extensive drug resistance (pXDR) was found in 2.3% of isolates. High single resistance was observed for oxytetracycline (93%) and amoxicillin (91.9%), followed by ampicillin (89.5%), trimethoprim–sulfamethoxazole, and pefloxacin (88.4%), and tetracycline (84.9%). Most importantly, 89.6% of isolates were resistant to carbapenems. All the isolates were positive for the blaTEM gene. However, the blaSHV and blaCTX-M-2 genes were identified in two ESBL-non producer isolates. None of the isolates carried the blaCTX-M-1 gene. This study provided evidence of the existence of MDR and pXDR ESBL-Ec in frozen chicken meat in Bangladesh, which may pose a risk to human health if the meat is not properly cooked or pickled raw only. This emphasizes the importance of the implementation of good slaughtering and processing practices by the processors.

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Antimicrobial resistance profile of Escherichia coli isolates recovered from diarrheic patients at Selam Health Center, Addis Ababa, Ethiopia

African Journal of Microbiology Research ◽

10.5897/ajmr2018.9030 ◽

2019 ◽

Vol 13 (26) ◽

pp. 457-463

Author(s):

Aseffa Negeri Abebe ◽

Tigabu Seyoum Eyasu ◽

Abubeker Ibrahim Rajiha ◽

Mamo Hassen

Keyword(s):

Escherichia Coli ◽

Antimicrobial Resistance ◽

Health Center ◽

Addis Ababa ◽

Resistance Profile ◽

Antimicrobial Resistance Profile

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Phylogenetic analysis, biofilm production, and antimicrobial resistance profile of Escherichia coli isolated from slaughtered pigs

Arquivo Brasileiro de Medicina Veterinária e Zootecnia ◽

10.1590/1678-4162-9926 ◽

2018 ◽

Vol 70 (4) ◽

pp. 1309-1315

Author(s):

A.S. Santos ◽

D.C.V. Lima ◽

E.F.T.S. Fernandes ◽

P.P.F. Albuquerque ◽

G.V. Gouveia ◽

...

Keyword(s):

Escherichia Coli ◽

Antimicrobial Resistance ◽

Phylogenetic Group ◽

Swine Industry ◽

Resistance Profile ◽

Enteric Diseases ◽

Biofilm Production ◽

Diffusion Technique ◽

Antimicrobial Resistance Profile ◽

Resistance Rates

ABSTRACT Enteric diseases of bacterial origin are frequent in the pig industry, of particular notoriety are the colibacillosis that mainly affect piglets and cause great damage to the swine industry worldwide. The aim of the study was to analyze phylogenetics, to detect biofilm production, and to determine antimicrobial resistance profile in 126 strains of Escherichia coli isolated from swabs obtained from fragments of the small intestines of 235 healthy pigs killed in slaughterhouses in Pernambuco (Brazil) using polymerase chain reaction (PCR), adherence to microplates test and disc diffusion technique. Of the analyzed samples, 88.10% (111/126) were classified in phylogenetic group B1; 4.76% (6/126) in group D; 3.97% (5/126) in group B2 and, 3.17% (4/126) in group A. Antimicrobial resistance rates observed were: lincomycin 100% (126/126), erythromycin 100% (126/126), chlortetracycline 94.44% (119/126), cephalothin 51.59% (65/126), ampicillin 38.89% (49/126), sulfamethoxazole + trimethoprim 37.3% (47/126), ciprofloxacin 19.84% (25/126), norfloxacin 14.29% (18/126), gentamicin 8.73% (11/126) and, chloramphenicol 5.55% (7/126). Multiple antibiotic resistance (MAR) ranged from 0.2 to 0.9. Of the strains tested 46.03% (58/126) produced biofilm, and 99.21% (125/126) of the strains exhibited multi-resistance. Further studies are required to elucidate the importance of each phylogenetic group in pigs and to prevent the propagation of multi-resistant E. coli strains.

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