Análise da proliferação e morfologia de mioblastos C2C12 durante o processo de diferenciação

Introdução: a miogênese é um processo essencial para a regeneração do tecido muscular após lesão. Objetivo: analisar a morfologia e a proliferação de mioblastos C2C12 durante o processo de diferenciação. Metodologia: os mioblastos foram cultivados em meio de manutenção (MM) (DMEM com 10% de SFB) e induzidos à diferenciação pela substituição por meio de diferenciação (MD) (DMEM com 2% soro de cavalo). A morfologia e proliferação celular foram avaliadas após 24, 48 e 96h de indução de diferenciação. A proliferação celular foi avaliada pelo método de MTT e as células foram fotografadas para análise da morfologia. Resultados: as células em MM apresentaram aumento significativo na proliferação quando comparadas às células em MD após 48 e 96h sendo observada uma maior confluência nestes mesmos períodos sem alteração da morfologia celular. Conclusão: os resultados podem facilitar no estabelecimento do período de análise para analisar a influência de algum tratamento sobre a proliferação e diferenciação de células musculares precursoras.Palavras-chave: Morfologia. Proliferação. Diferenciação. Mioblastos. Analysis of proliferation and morphology of C2C12 during cell differentiationBackground: The myogenesis is a process essential for the repair of injured muscle fibers. Objective: to analyze the morphology and proliferation of C2C12 myoblasts during the differentiation process induced by horse serum. Methodology: The myoblasts were cultured in a culture of “Eagle” modified by Dulbecco, containing 10% fetal bovine serum (FBS), were induced to differentiate by replacing by DMEM containing 2% horse serum. Cells grown in DMEM containing 10% FBS analyzed in the same period served as controls. The morphology was assayed at 24, 48 and 96h after the addition of 2% horse serum or only during maintenance in DMEM 10% FCS. Results: The cells were photographed and the proliferation was assessed by MTT. Conclusion: our data can facilitate the establishment the best period to be used in order to analyze the influence of any treatment on the proliferation and differentiation of muscle cells.Keywords: Morphology. Proliferation. Differentiation. Myoblasts.

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Depleting extracellular vesicles from fetal bovine serum alters proliferation and differentiation of skeletal muscle cells in vitro

BMC Biotechnology ◽

10.1186/s12896-016-0262-0 ◽

2016 ◽

Vol 16 (1) ◽

Cited By ~ 25

Author(s):

Hala Aswad ◽

Audrey Jalabert ◽

Sophie Rome

Keyword(s):

Skeletal Muscle ◽

Extracellular Vesicles ◽

Bovine Serum ◽

Fetal Bovine Serum ◽

Muscle Cells ◽

Skeletal Muscle Cells ◽

Proliferation And Differentiation ◽

Fetal Bovine

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Effects of Luteolin on Fetal Bovine Serum-induced Events in Cultured Rat Vascular Smooth Muscle Cells

Journal of Life Science ◽

10.5352/jls.2012.22.12.1595 ◽

2012 ◽

Vol 22 (12) ◽

pp. 1595-1599

Author(s):

Yong Lim

Keyword(s):

Smooth Muscle ◽

Vascular Smooth Muscle ◽

Smooth Muscle Cells ◽

Vascular Smooth Muscle Cells ◽

Bovine Serum ◽

Fetal Bovine Serum ◽

Muscle Cells ◽

Fetal Bovine

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Human Serum is as Efficient as Fetal Bovine Serum in Supporting Proliferation and Differentiation of Human Multipotent Stromal (Mesenchymal) Stem Cells In Vitro and In Vivo

Stem Cell Reviews and Reports ◽

10.1007/s12015-011-9274-2 ◽

2011 ◽

Vol 7 (4) ◽

pp. 860-868 ◽

Cited By ~ 50

Author(s):

Abdullah Aldahmash ◽

Mandana Haack-Sørensen ◽

May Al-Nbaheen ◽

Linda Harkness ◽

Basem M. Abdallah ◽

...

Keyword(s):

Stem Cells ◽

Mesenchymal Stem Cells ◽

Human Serum ◽

Bovine Serum ◽

Fetal Bovine Serum ◽

Proliferation And Differentiation ◽

Fetal Bovine

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Effects of heparin-binding substances in fetal bovine serum upon proliferation and differentiation of trophoblast cells in the mouse blastocysts cultured in vitro

Placenta ◽

10.1016/0143-4004(94)90202-x ◽

1994 ◽

Vol 15 (7) ◽

pp. A81

Author(s):

Hideki Koi ◽

Yasusi Shimizu ◽

Shuuiti Sakamoto ◽

Chikashi Tachi ◽

Takeshi Aso

Keyword(s):

Bovine Serum ◽

Fetal Bovine Serum ◽

Heparin Binding ◽

Trophoblast Cells ◽

Proliferation And Differentiation ◽

Fetal Bovine

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Adsorption Behavior of Albumin and other Proteins on Carbon Nanotubes Studied by Chromatography

Key Engineering Materials ◽

10.4028/www.scientific.net/kem.529-530.615 ◽

2012 ◽

Vol 529-530 ◽

pp. 615-620

Author(s):

M. Morikawa ◽

Y. Kuboki ◽

Tsukasa Akasaka ◽

Shigeaki Abe ◽

H. Takita ◽

...

Keyword(s):

Carbon Nanotubes ◽

Bovine Serum ◽

Acid Treatment ◽

Fetal Bovine Serum ◽

Adsorption Behavior ◽

Sem Images ◽

Treatment Conditions ◽

Cell Proliferation And Differentiation ◽

Proliferation And Differentiation ◽

Fetal Bovine

For application of carbon nanotubes (CNTs) as biomaterials, it is important to clarify the interaction between CNTs and proteins, which may affect on cell proliferation and differentiation. In this study, the adsorption behavior was investigated for representative proteins, bovine serum albumin, lysozyme and fetal bovine serum by using chromatography system. It was also aimed whether the different treatment conditions of CNTs affected the adsorption of proteins. CNTs used for this study had a straight shape and about 70nm in a diameter from SEM observation. There was not much difference between untreated and treated CNTs from SEM images. In chromatography experiments, all the proteins of albumin, lysozyme and serum were eluted immediately after injected to the column of untreated CNTs. Second elution appeared after buffer was changed from phosphate saline buffer to 25mM sodium hydroxide (NaOH). The same tendency was confirmed for CNTs with only acid treatment. However the eluted peaks became remarkably smaller after the column was changed to CNTs with thermal and twice acid treatments. These results indicated that treatment conditions for CNTs affected the adsorption behavior of proteins.

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