ONTOGENETIC ASPECTS OF MELATONIN RECEPTORS 1A DENSITY IN THE LATERAL PREOPTIC NUCLEUS OF THE HYPOTHALAMUS UNDER VARIOUS LIGHT CONDITIONS

The aim: To find out density of melatonin receptors 1A in the neurons of the lateral preoptic nucleus of the hypothalamus in mature and old rats under various light conditions. Materials and methods: The study was carried out on 72 albino mature and old rats with light conditions appropriate for the experiment. To find out circadian differences of melatonin receptors 1A the material for the study was taken at 2 p.m. and 2 a.m. Visualization of primary antibodies against melatonin receptors 1A (Abcam) was conducted by means of the polymeric system Dako and diaminobenzidine staining under the microscope Delta Optical Evolution 100. The intensity of staining was assessed on the digital copies of images according to computer microdensitometry method. Results: Immunohistochemical examinations conducted enable to suggest that melatonin receptors 1A in the neurons of the lateral preoptic nucleus of the hypothalamus respond to different light conditions. In particular, intensity of immunohistochemical staining to melatonin receptors 1A under conditions of light deprivation increases both in mature and old rats, but it decreases under conditions of light stimulation. The parameter is higher at 2 a.m. as compared with 2 p.m. Intensity of immunohistochemical staining to melatonin receptors 1A in the neurons of the lateral preoptic nucleus of the hypothalamus is always lower in old rats than in mature ones. Conclusions: Density of melatonin receptors 1A in the neurons of the lateral preoptic nucleus of the hypothalamus is subordinated to the circadian rhythm: it increases at night and decreases in the daytime. At the same time, light stimulation results in disorders of the rhythm and development of desynchronization.

INFLUENCE OF PHASE STRUCTURE POLYMERIC FILMFORMER ON PHYSICAL MECHANICAL AND WATER RESISTANT PROPERTIES OF COATINGS

The phase structure, adhesion and physical mechanical properties, water resistance of varnish coatings on the basis of the modified and not modified epoxy filmformers, with organic silicon ammine «AСOT-2» and low-molecular «ПO-200» polyamide as curing agents. It is established that the process of structurization of unfilled epoxy compositions depends on each component of polymeric system. The received results of determination of adhesion, physical mechanical properties, water resistance correlate with results of researches of phase structure of the coatings received on the basis of modified and not modified epoxy compositions, with organic silicon ammine «AСOT-2» and low-molecular «ПO-200» polyamide as curing agents.

PEGylated ethyl cellulose micelles as a nanocarrier for drug delivery

Ethyl cellulose was developed as an amphiphilic polymer by PEGylation and fabricated as nanomicelles for delivery of active molecules. This polymeric system can be used as next generation nano drug delivery system (nanoDDS) for cancer therapy.