Computed Microtomography (Micro-CT) in the Anatomical Study and Identification of Solenogastres (Mollusca)

Solenogastres are vermiform marine molluscs characterised by an aculiferous mantle, a longitudinal ventral pedal groove and a terminal or subterminal pallial cavity. Their classification is based in part on the type of mantle sclerites, but identification to even the family level generally requires the study of internal anatomical characters. Taxonomically important internal characters include those related to radular structure, the type of ventrolateral glandular organs of the pharynx and the reproductive system, among others. In order to study their internal anatomical organisation, according to the classical reconstruction method, serial histological sections of specimens are made, from which the 2D internal anatomy of the specimen can be reconstructed manually. However, this is a time-consuming technique that results in destruction of the specimen. Computed microtomography or micro-CT is a non-destructive technique based on the measurement of the attenuation of X-rays as they pass through a specimen. Micro-CT is faster than histology for studying internal anatomy and it is non-destructive, meaning that specimens may be used for e.g., DNA extraction or retained as intact vouchers. In this paper, the utility of micro-CT for studying taxonomically important internal anatomical structures was assessed. Results of the 3D anatomical study of the soft parts of four specimens of three species using micro-CT are presented: Proneomenia sluiteriHubrecht, 1880, Dorymenia menchuescribanaeGarcía-Álvarez et al., 2000 and Anamenia gorgonophilaKowalevsky, 1880. Micro-CT enabled detailed study of most taxonomically important anatomical characters, precise measurements of structures, and observation of the relative position of organs from a variety of angles. However, it was not possible to observe the radula and some details of the ventral foregut organs could not be discerned. Despite these limitations, results of this study highlight micro-CT as a valuable tool to compliment histology in the study of solenogaster anatomy and in non-destructively identifying animals to the family and even genus-level.

Taungurungia gen. nov., from the Lower Devonian of Yea, central Victoria, Australia

This paper records a new genus Taungurungia, which is the first new taxon with emergences to be described from the Lower Devonian of Victoria. The fossil is preserved primarily as a compression and impression, and lacks internal anatomy. The fossil extends our knowledge of known variations within early land plants, with most characteristics, such as emergences and H- or K-branching, redolent of affinities with the zosterophylls. However, having a large ovate terminal sporangium, the fossil adds to taxa that in some cases have been provisonally allied to the zosterophylls with elongate sporangia; this further demonstrates the need for reassessment of the Zosterophyllopsida.

The natural history of the Stenoderminae Chiroderma doriae vizottoi Taddei and Lim 2010 (Chiroptera, Phyllostomidae) in a semiarid region from Brazil

Original data on diet, internal anatomy, morphology, reproduction, and parasites of Chiroderma doriae vizottoi from the State of Ceará (Brazil) are presented. Intact and crushed seeds of Solanum rhytidoandrum and scales of Lepidoptera were detected in the gastrointestinal tract and feces. Observation of internal organs did not reveal any abnormalities; the intestines were, on average, 11 times longer than the animal’s body length. Reproduction seems to occur in the rainy season. The association with an ectoparasite, Mastoptera sp. (Diptera, Streblidae), was recorded.

INTERNAL ANATOMY OF THE OLIVE TURTLE (Lepidochelys olivacea)

In this article, the internal organs of an olive sea turtle (Lepidochelys olivacea) were described. The autopsy was based on a methodological guide of “Virchow”. The Cardiorespiratory, Digestive, Liver, Gallbladder, Pancreas, Spleen, Genito-Urinary System, and Endocrine System systems were observed. This description of the internal organs aimed to provide practice in anatomical identification and to provide didactic information for the students of the course in veterinary medicine.

A new species of Polyophthalmus (Annelida, Opheliidae) from the Arabian Gulf, with an insight on internal anatomy and diversity of the genus

Polyophthalmus zhadanae sp. nov. (Annelida, Opheliidae), is described from the coast of Kuwait and Saudi Arabia (Arabian Gulf) after specimens collected in the intertidal and shallow subtidal. This new species is characterised by the body pigmentation pattern, distribution of lateral eyes, presence of small dorsal and ventral parapodial cirri and pygidial features. External and internal anatomy was described after examination with SEM and micro-CT. The presence of several body structures in the genus such as a cephalic palpode-like papilla is discussed. Furthermore, the diversity of genus Polyophthalmus and the presence of Polyophthalmus pictus (Dujardin, 1839) in the Indo-Pacific is reviewed and a key to species is provided.

Revealing morphological characteristics of Goniodorididae genera (Mollusca: Nudibranchia)

Detailed knowledge of the anatomy of the species is an essential element in taxonomic studies, since it allows the comparison and differentiation of separate groups of taxa. It becomes especially important when considering type species, as the subsequent identification of the species that compose the taxa is based on its characteristics, considered common in the group. However, despite its relevance, there are still numerous species without detailed descriptions, being especially significant among invertebrates. The family Goniodorididae is a little-known group of nudibranchs that includes eight recognized genera: Okenia, Goniodoris, Ancula, Lophodoris, Spahria, Trapania, Goniodoridella and Murphydoris. Several of their species are not completely described, including type species, and the systematics of the family is still unclear. Here we study in detail the external morphology and internal anatomy of the type species of five of the eight Goniodorididae genera using microcomputed tomography and scanning electron microscopy. We include the species Okenia elegans, Goniodoris nodosa, Ancula gibbosa, Goniodoridella savignyi and Murphydoris singaporensis as well as one species of Trapania, T. graeffei. We describe for the first time the detailed internal anatomy of the type species Goniodoridella savignyi. The diagnostic features of each genus are compared, and a preliminary framework is shown to clarify their systematics and identifications.

Endodontic management of multiple roots and root canals in mandibular canine: A case report series

Successful endodontic treatment depends upon the clinician’s precise knowledge and ability to manage complex internal anatomy in order to avoid failure during endodontic treatment. Mandibular canines normally have single root with centrally placed single canal and internal anatomy as simple as could be expected, but there are such canines with a single root with two canals, two roots or fused roots. The occurrence of two canal joining to one for a men may be a rare entity with 4-15% prevalence. Canine is the cornerstone of mouth due to its position and very important as abutment for any type of restoration, so long term success of the restorative depend directly on the quality of endodontic treatment. This article presents a case series of successful endodontic management of mandibular canine with two canals which merge into one canal with one foramina and mandibular canine with two roots and two canals.

Bypassing a Separated Instrument in Mandibular First Premolar with Two Canals – A Case Report

Fracture of an endodontic instrument will impair the debridement procedure and thus affects the endodontic outcome. This case report focuses on the management of separated instrument and highlights the anatomical variation of mandibular first premolar. An in-depth knowledge on the internal anatomy of this particular tooth as well as following a proven and safe technique as a guideline is important in preventing these endodontic mishaps.