A constraint on post–6 Ma timing of western Grand Canyon (Arizona, USA) incision removed: Local derivation indicated by ca. 5.4 Ma fluvial deposits below Shivwits Plateau basalts north of Grand Canyon

The timing of integration of the Colorado River system is central to understanding the landscape evolution of much of the southwestern United States. However, the time at which the Colorado River started incising the westernmost Grand Canyon (Arizona) is still an unsettled question, with conflicting interpretations of both geologic and thermochronologic data from western Grand Canyon. Fluvial gravels on the Shivwits Plateau, north of the canyon, have been reported to contain clasts derived from south of the modern canyon, suggesting the absence of western Grand Canyon at the time of their deposition. In this study, we reassess these deposits using modern geochronologic measurements to determine the age of the deposits and the presence or absence of clasts from south of the Grand Canyon. We could not identify southerly derived clasts, so cannot rule out the existence of a major topographic barrier such as Grand Canyon prior to the age of deposition of the gravels. 40Ar/39Ar analysis of a basalt clast entrained in the upper deposit (in combination with prior data) supports a maximum age of deposition of ca. 5.4 Ma, limiting deposition to post-Miocene, a period from which very few diagnostic and dated fluvial deposits remain in the western Colorado Plateau. Analysis of detrital zircon composition of the sand matrix supports interpretation of the deposit as being locally derived and not part of a major throughgoing river. We suggest that the published constraint of <6 Ma timing of Grand Canyon incision may be removed, given that no clasts that must be sourced from south of Grand Canyon were found in the only known outcrop of gravels under the Shivwits Plateau basalts at Grassy Mountain north of Grand Canyon.

Genetic and phenotypic characterization of the fungal pathogen Cytospora plurivora from western Colorado peach orchards and the development of a ddPCR assay for detection and quantification

Cytospora canker is one of the most important diseases affecting peach production in Colorado, yet previous efforts to characterize Cytospora species diversity in Colorado have relied exclusively on morphological traits. Recently, several new Cytospora species were described from peach orchards within the United States using molecular and morphological data, prompting the need to reexamine Cytospora spp. present on peach trees in Colorado. A total of 137 isolates of Cytospora spp. were collected from eight orchards in western Colorado. Isolates were sequenced at the ITS (internal transcribed spacer) region and EF1a (elongation factor 1 alpha) and assessed with reference sequences in phylogenetic analyses. All isolates from western Colorado peach trees resolved with the newly described Cytospora plurivora. In addition to molecular characterization, temperature growth and virulence assays were conducted to assess phenotypic variation among the isolates from western Colorado. Variation across isolates was found both in growth at different temperatures and in virulence. Ancestral state reconstruction analyses resolved the most virulent (and most often collected) haplotypes together in a well-supported clade from which a single monophyletic origin of high virulence can be inferred. Finally, a droplet digital PCR assay was developed for use in ongoing and future studies to detect and quantify C. plurivora from field and laboratory samples.

First records, phenology, habitat, and host-plant associations of Macrotera opuntiae (Cockerell) (Hymenoptera: Andrenidae) in Montana

Macrotera (Cockerellula) opuntiae (Cockerell) is a minute bee oligolectic on cacti and one of the few stone-nesting bee species. We document the first records of M. opuntiae in Montana, USA, a considerable range expansion from where this species was previously known in Colorado. We briefly describe the habitat and host-plant (cacti) associations, and the activity period of M. opuntiae adults in Montana. A new state record for Nebraska and new records for western Colorado are also reported.

A Rare Ice Storm in the Colorado Rockies

During the early morning hours of 9 January 2017, freezing rain developed across several valley locations in western Colorado. The resultant ice accumulation led to extremely treacherous travel conditions with hundreds of vehicle accidents reported in the vicinity of Grand Junction, Colorado and near Durango, Colorado. Additionally, widespread power outages were reported in Durango and near Steamboat Springs, Colorado. First responders were overwhelmed by the volume increase of emergency calls, and secondary services were requested from nearby municipalities to help with the increased workload. The emergency operations center in Mesa County, Colorado (Grand Junction) was activated as a result of the numerous accidents and injuries across the region. An ice storm of this magnitude has not been experienced in Grand Junction’s period of record, which dates back to 1893. A detailed investigation explores the physical processes responsible for this ice storm over the complex terrain of the Intermountain West.

First Report of Beet Curly Top Virus Infecting Industrial Hemp (Cannabis sativa) in Arizona

Industrial hemp (Cannabis sativa) is an emerging crop in Arizona, with many uses, including fiber, cosmetic products, and health food. In 2020, severe curly top disease outbreaks were observed in several hemp fields in Yuma and Graham Counties, Arizona, where disease incidence and severity were considerably high, up to 100% crop loss occurring in some fields. A wide range of symptoms have been observed at different infection stages and plant growth stages at the time of infection. Early stage symptoms manifest as light green-to-yellowing of new growth, similar to sulfur or micronutrient deficiency, usually combined with older leaves with dark green “blotchy” mosaic mottling overlaying light green chlorosis. Mosaic mottling of older leaves continues into mid-growth stage, and is coupled with more severe yellowing and witch’s broom (stunted leaves and shortened internode length of stem) of apical meristematic tissue. Curling and twisting of new leaves has also been observed. Symptoms often appear to be isolated to individual branches, with other branches showing no visual symptoms, often outgrowing and covering affected branches until harvest. Late stage symptoms include severe leaf curling with or without twisting, continued stunting, and necrosis of yellow leaves, resulting in significant yield reduction. Severely affected plants dwarfed by the virus experienced high mortality rates later into the season, most likely attributed to reduced ability to overcome abiotic stress conditions. These symptoms indicated the likelihood of curly top caused by Beet curly top virus (BCTV), which has been recently reported in Colorado (Giladi et al., 2020). Shoots were collected from thirty-eight symptomatic and nine asymptomatic hemp plants from July to August, 2020. Leaves were also collected as positive control from four chili pepper plants with or without curly top symptoms in Cochise County. Genomic DNA was extracted using DNeasy Plant Pro Kit (Qiagen Inc., Valencia, CA) according to the manufacturer’s instructions. BCTV-specific primers BCTV1 and BCTV2 were used to detect BCTV following a method by Rondon (Rondon et al., 2016). A 500 bp DNA fragment, indicative of BCTV, was amplified from all symptomatic hemp and chili pepper samples, but not from asymptomatic samples. Sequence analysis of this 500 bp DNA fragment revealed 98.99 % identity with GenBank accession MK803280, which is Beet curly top virus isolate from hemp identified in Western Colorado (Giladi et al., 2020). The full-length genomes of BCTV isolates from hemp and chili peppers were generated with additional primers 328F/945R (620bp), 455F/ 945R (490bp), OutR/ 2213F (1,190bp), 2609R/ 1278R (1,340bp), BCTV2/ 2609R (1,890bp) (Rondon et al., 2016, Strausbaugh et al., 2008). The complete nucleotide sequence (MW182244) from hemp was 2,929 bp and had 99.35% sequence identity with GenBank accession KX867055, which was a Worland strain of Beet curly top virus isolated from an Idaho sugar beet plant (Strausbaugh et al., 2017). Our hemp BCTV genome sequences shared 96.08% identity with the hemp strain of BCTV from Colorado (MK803280) and 99.50% identity with the BCTV isolate (MW188519) from chili pepper identified in this study. BCTV was reported on outdoor hemp in Western Colorado, in 2020 (Giladi et al., 2020). This is the first report of BCTV in Arizona causing curly top of industrial hemp in the field. In Arizona, BCTV is widespread on many agronomic crops including chili peppers and spread primarily by the phloem-feeding beet leafhoppers: Circulifer tenellus (Hemiptera: Cicadellidae) (Bennett, 1967). Due to the wide distribution of beet leafhoppers and abundant range of host plants for the virus, BCTV may become one of the most yield-limiting factors affecting the emerging industrial hemp production systems in Arizona.