Background: Though mostly needle stick injuries cause just minor trauma and bleeding, yet, even without trauma and bleeding, the danger of contamination and infection remains. Thus, a needle stick injury may represent a hazard for a patient if the injured health professional carries hepatitis B virus, hepatitis C virus or human immunodeficiency virus. Objectives: To assess the effectiveness of teaching programme on knowledge of GNM-II year students regarding prevention of needle stick injuries at Ancillary Medical Training School, Shireen Bagh, Srinagar. Methods: In this study, a total of 50 GNM - II year students were included fulfilling the inclusion and exclusion criteria. A self-structured knowledge questionnaire was used as the research tool. Demographic variables such as place of residence, educational status of father, educational status of mother, occupation of father, occupation of mother, and previous exposure to information related to needle stick injuries were obtained. Results: The findings of the present study revealed that there was a statistically significant association between the pre-test knowledge scores of study subjects with and previous exposure to information (p = 0.007) while no significant association was found between the pre-test knowledge scores of study subjects and other demographic variables like residence (p = 0.785), educational status of father (p = 0.435), educational status of mother (p = 0.345), occupation of father (p = 0.851) and occupation of mother (p = 0.881). Conclusion: This study reveals the importance of educational programmes, skill training programmes, conferences, workshops and educational meetings to enhance the nursing students’ knowledge regarding the prevention of needle stick injuries.