Surface-Layer Circulations in Suruga Bay Induced by Intrusions of Kuroshio Branch Water

The water mass structure in Suruga Bay is strongly influenced by open-ocean water. In particular, it is suggested that intermittent intrusions of the Kuroshio water generate characteristic circulations in the surface layer of the bay. In this study, we investigated the processes of the intrusions of open-ocean water into the bay and related generation of bay-scale cyclonic and anti-cyclonic circulation patterns. In doing so, we used an ocean simulation product with observational data constraint on meso and larger scales and with a resolution fine enough to resolve the smaller-scale intrusion structure. Cyclonic and anti-cyclonic circulation patterns as suggested by previous observational studies were detected as positive and negative first leading empirical orthogonal function (EOF) modes of the velocity field in Suruga Bay. The time scale of occurrences of these patterns was estimated as about 1 month, which was consistent with short-term Kuroshio fluctuations as reported in previous studies. Conditions favorable for generating these patterns were analyzed for three typical Kuroshio path periods individually. As suggested by previous studies, relatively strong northward flow to the west of Zeni-su generally promoted the open-ocean water intrusions into the eastern bay mouth, leading the cyclonic circulation in Suruga Bay. Our results showed that the correlation of this relation was significant for each Kuroshio path period. The open-ocean water intrusion increased the surface-layer temperature in Suruga Bay by about 0.7°C on average. On the other hand, the anti-cyclonic circulation pattern in Suruga Bay tended to be generated with relatively weak northward flow to the west of Zeni-su during the large meander Kuroshio path period, whereas this relation was rather weak during other periods. These results were mostly supported by available observations and would be useful for integrating our understanding of the influences of the western boundary current fluctuations on the circulation and temperature variations in proximal bays.

First record of swimming speed of the Pacific sleeper shark Somniosus pacificus using a baited camera array

The Pacific sleeper shark Somniosus pacificus is one of the largest predators in deep Suruga Bay, Japan. A single individual of the sleeper shark (female, ~300 cm in total length) was observed with two baited camera systems deployed simultaneously on the deep seafloor in the bay. The first arrival was recorded 43 min after the deployment of camera #1 on 21 July 2016 at a depth of 609 m. The shark had several remarkable features, including the snout tangled in a broken fishing line, two torn anteriormost left-gill septums, and a parasitic copepod attached to each eye. The same individual appeared at camera #2, which was deployed at a depth of 603 m, ~37 min after it disappeared from camera #1 view. Finally, the same shark returned to camera #1 ~31 min after leaving camera #2. The distance between the two cameras was 436 m, and the average groundspeed and waterspeed of the shark were 0.21 and 0.25 m s−1, respectively, which were comparable with those of the Greenland shark Somniosus microcephalus (0.22–0.34 m s−1) exhibiting the slowest comparative swimming speed among fish species adjusted for size. The ambient water temperature of the Pacific sleeper shark was 5.3 °C, which is considerably higher than that of the Greenland shark (~2 °C). Such a low swimming speed might be explained by the ‘visual interactions hypothesis’, but it is not a consequence of the negative effects of cold water on their locomotor organs.

Carotenoid Nostoxanthin Production by Sphingomonas sp. SG73 Isolated from Deep Sea Sediment

Carotenoids are used commercially for dietary supplements, cosmetics, and pharmaceuticals because of their antioxidant activity. In this study, colored microorganisms were isolated from deep sea sediment that had been collected from Suruga Bay, Shizuoka, Japan. One strain was found to be a pure yellow carotenoid producer, and the strain was identified as Sphingomonas sp. (Proteobacteria) by 16S rRNA gene sequence analysis; members of this genus are commonly isolated from air, the human body, and marine environments. The carotenoid was identified as nostoxanthin ((2,3,2′,3′)-β,β-carotene-2,3,2′,3′-tetrol) by mass spectrometry (MS), MS/MS, and ultraviolet-visible absorption spectroscopy (UV-Vis). Nostoxanthin is a poly-hydroxy yellow carotenoid isolated from some photosynthetic bacteria, including some species of Cyanobacteria. The strain Sphingomonas sp. SG73 produced highly pure nostoxanthin of approximately 97% (area%) of the total carotenoid production, and the strain was halophilic and tolerant to 1.5-fold higher salt concentration as compared with seawater. When grown in 1.8% artificial sea salt, nostoxanthin production increased by 2.5-fold as compared with production without artificial sea salt. These results indicate that Sphingomonas sp. SG73 is an efficient producer of nostoxanthin, and the strain is ideal for carotenoid production using marine water because of its compatibility with sea salt.

Discovery of a colossal slickhead (Alepocephaliformes: Alepocephalidae): an active-swimming top predator in the deep waters of Suruga Bay, Japan

A novel species of the family Alepocephalidae (slickheads), Narcetes shonanmaruae, is described based on four specimens collected at depths greater than 2171 m in Suruga Bay, Japan. Compared to other alepocephalids, this species is colossal (reaching ca. 140 cm in total length and 25 kg in body weight) and possesses a unique combination of morphological characters comprising anal fin entirely behind the dorsal fin, multiserial teeth on jaws, more scale rows than congeners, precaudal vertebrae less than 30, seven branchiostegal rays, two epurals, and head smaller than those of relatives. Mitogenomic analyses also support the novelty of this large deep-sea slickhead. Although most slickheads are benthopelagic or mesopelagic feeders of gelatinous zooplankton, behavioural observations and dietary analyses indicate that the new species is piscivorous. In addition, a stable nitrogen isotope analysis of specific amino acids showed that N. shonanmaruae occupies one of the highest trophic positions reported from marine environments to date. Video footage recorded using a baited camera deployed at a depth of 2572 m in Suruga Bay revealed the active swimming behaviour of this slickhead. The scavenging ability and broad gape of N. shonanmaruae might be correlated with its colossal body size and relatively high trophic position.

16S rRNA Gene Amplicon Sequencing of Gut Microbiota in Three Species of Deep-Sea Fish in Suruga Bay, Japan

ABSTRACT We report here 16S rRNA gene amplicon sequence analysis of the gut microbiota in three species of deep-sea fish collected from Suruga Bay, Japan. Of the three species, two were dominated by the phylum Proteobacteria (genus Photobacterium), while one was dominated by the phyla Spirochaetes (genus Brevinema) and Tenericutes (unclassified Mycoplasmataceae).