scholarly journals Robust hemostatic bandages based on nanoclay electrospun membranes

2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Yan Cui ◽  
Zongwang Huang ◽  
Li Lei ◽  
Qinglin Li ◽  
Jinlong Jiang ◽  
...  
Keyword(s):  
Traffic Accidents ◽  
Massive Bleeding ◽  
Electrospun Membrane ◽  
Acute Hemorrhage ◽  
Functional Sites ◽  
Military Conflicts ◽  
Electrospun Membranes ◽  
Good Biocompatibility

AbstractDeath from acute hemorrhage is a major problem in military conflicts, traffic accidents, and surgical procedures, et al. Achieving rapid effective hemostasis for pre-hospital care is essential to save lives in massive bleeding. An ideal hemostasis material should have those features such as safe, efficient, convenient, economical, which remains challenging and most of them cannot be achieved at the same time. In this work, we report a rapid effective nanoclay-based hemostatic membranes with nanoclay particles incorporate into polyvinylpyrrolidone (PVP) electrospun fibers. The nanoclay electrospun membrane (NEM) with 60 wt% kaolinite (KEM1.5) shows better and faster hemostatic performance in vitro and in vivo with good biocompatibility compared with most other NEMs and clay-based hemostats, benefiting from its enriched hemostatic functional sites, robust fluffy framework, and hydrophilic surface. The robust hemostatic bandages based on nanoclay electrospun membrane is an effective candidate hemostat in practical application.

scholarly journals Lithium-Doped Biological-Derived Hydroxyapatite Coatings Sustain In Vitro Differentiation of Human Primary Mesenchymal Stem Cells to Osteoblasts

Coatings ◽  
2019 ◽  
Vol 9 (12) ◽  
pp. 781 ◽  
Author(s):  
Paula E. Florian ◽  
Liviu Duta ◽  
Valentina Grumezescu ◽  
Gianina Popescu-Pelin ◽  
Andrei C. Popescu ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Doping Agent ◽  
Hydroxyapatite Coatings ◽  
3D Network ◽  
Immunofluorescence Labelling ◽  
Good Biocompatibility ◽  
Adhesion Process

This study is focused on the adhesion and differentiation of the human primary mesenchymal stem cells (hMSC) to osteoblasts lineage on biological-derived hydroxyapatite (BHA) and lithium-doped BHA (BHA:LiP) coatings synthesized by Pulsed Laser Deposition. An optimum adhesion of the cells on the surface of BHA:LiP coatings compared to control (uncoated Ti) was demonstrated using immunofluorescence labelling of actin and vinculin, two proteins involved in the initiation of the cell adhesion process. BHA:LiP coatings were also found to favor the differentiation of the hMSC towards an osteoblastic phenotype in the presence of osteoinductive medium, as revealed by the evaluation of osteoblast-specific markers, osteocalcin and alkaline phosphatase. Numerous nodules of mineralization secreted from osteoblast cells grown on the surface of BHA:LiP coatings and a 3D network-like organization of cells interconnected into the extracellular matrix were evidenced. These findings highlight the good biocompatibility of the BHA coatings and demonstrate that the use of lithium as a doping agent results in an enhanced osteointegration potential of the synthesized biomaterials, which might therefore represent viable candidates for future in vivo applications.

scholarly journals Efficient Photoacoustic Imaging With Biomimetic Mesoporous Silica-Based Nanoparticles

2021 ◽  
Vol 9 ◽  
Author(s):  
Chuangjia Huang ◽  
Xiaoling Guan ◽  
Hui Lin ◽  
Lu Liang ◽  
Yingling Miao ◽  
...  
Keyword(s):  
Mesoporous Silica ◽  
Near Infrared ◽  
Ex Vivo ◽  
Photoacoustic Imaging ◽  
Mesoporous Silica Nanoparticles ◽  
Good Biocompatibility ◽  
Targeting Ability ◽  
Body Clearance

Indocyanine green (ICG), a near-infrared (NIR) fluorescent dye approved by the Food and Drug Administration (FDA), has been extensively used as a photoacoustic (PA) probe for PA imaging. However, its practical application is limited by poor photostability in water, rapid body clearance, and non-specificity. Herein, we fabricated a novel biomimetic nanoprobe by coating ICG-loaded mesoporous silica nanoparticles with the cancer cell membrane (namely, CMI) for PA imaging. This probe exhibited good dispersion, large loading efficiency, good biocompatibility, and homologous targeting ability to Hela cells in vitro. Furthermore, the in vivo and ex vivo PA imaging on Hela tumor-bearing nude mice demonstrated that CMI could accumulate in tumor tissue and display a superior PA imaging efficacy compared with free ICG. All these results demonstrated that CMI might be a promising contrast agent for PA imaging of cervical carcinoma.

Ultra-bright near-infrared-emitting HgS/ZnS core/shell nanocrystals for in vitro and in vivo imaging

2015 ◽  
Vol 3 (34) ◽  
pp. 6928-6938 ◽  
Author(s):  
Jing Yang ◽  
Yaoping Hu ◽  
Jiangwei Tan ◽  
Li Jia ◽  
Yu-Hua Zhu ◽  
...  
Keyword(s):  
In Vivo Imaging ◽  
Near Infrared ◽  
Core Shell ◽  
Good Biocompatibility

Novel ultra-bright, stable NIR-emitting HgS/ZnS core/shell NCs with good biocompatibility for in vitro and in vivo imaging.

Ce-Based Nanoparticles Loaded with Cisplatin for Tumour Radiotherapy

2020 ◽  
Vol 16 (10) ◽  
pp. 1482-1494
Author(s):  
Li Sun ◽  
Chang Jiang ◽  
Wenhai Li ◽  
Zelai He ◽  
Gengming Wang ◽  
...  
Keyword(s):  
Photoelectric Effect ◽  
Inhibitory Effect ◽  
Precipitation Method ◽  
Treatment Mode ◽  
X Ray ◽  
Transmission Electron ◽  
Good Biocompatibility ◽  
Synergistic Inhibitory Effect

The combination of radiotherapy and chemotherapy is a common and useful treatment mode for tumours. But traditional methods inevitably lead to a variety of side effects. A drug delivery system (DDS), which has good biocompatibility and strong anti-tumour ability, is expected to solve this problem. Studies have shown that Ce-based nanoparticles (NPs) have good radiosensitization effect through the photoelectric effect. Hence, cisplatin-loaded LiLuF4 :Ce3+scintillation NPs (NP + Cis) were first constructed in this study, which was synthesized by the crystal precipitation method and characterized by transmission electron microscopy (TEM). Subsequently, its toxicity was verified, and the radiosensitization effect and basic radiosensitization mechanism on tumour cells and tumour-bearing mice were researched. Results showed that NP + Cis triggered massive DNA damage and effectively inhibited cell viability in vitro under the exposure of X-ray irradiation (IR). Moreover, the experiments in vivo showed that the NP + Cis had higher biosafety, which could absorb enough irradiation and produce a synergistic inhibitory effect on tumours through the releasing of Cis. NP + Cis can improve the performance of DDS in chemoradiotherapy.

scholarly journals In vivo and in vitro models of traumatic injuries of the spinal cord

2017 ◽  
Vol 5 (1) ◽  
pp. 87-93
Author(s):  
O. Rybachuk ◽  
I. Arkhypchuk ◽  
Yu. Lazarenko
Keyword(s):  
Spinal Cord ◽  
Spinal Cord Injury ◽  
Traffic Accidents ◽  
Experimental Models ◽  
The Body ◽  
Nerve Tissue ◽  
Economic Losses ◽  
Cord Injury

In recent years, there is a growing interest in the mechanisms of regeneration of damaged nerve tissue, including the spinal cord, as its injuries are quite common due to traffic accidents, industrial injuries and military actions. Damage to the spinal cord results in the loss of functional activity of the body below the injury site, which affects person’s ability to self-service and significantly reduces its efficiency. The effects of spinal injuries annually cause significant social and economic losses worldwide, including Ukraine. The development of new treatments for pathologies of the central nervous system requires mandatory pre-testing of their effectiveness in experiments in vitro and in vivo. Therefore, searching and creation of optimal animal model of spinal cord injury is in order to it meets most complete picture of the damage characteristic of real conditions in humans. This is an important task of modern neurophysiology. Such models can be used, primarily, for a more detailed clarification of the pathogenesis of all levels of nerve tissue damage and research of its own recovery potential by endogenous reparation mechanisms. In addition, experimental models allow to estimate the safety and predict the effectiveness of various therapeutic approaches to spinal cord injury.

The Effect Of Antiporcine Willebrand Factor Monoclonal Antibodies On The Bleeding Time

1981 ◽  
Author(s):  
E J W Bowie ◽  
D N Fass ◽  
J A Katzmann
Keyword(s):  
Monoclonal Antibodies ◽  
Bleeding Time ◽  
Vitro Assay ◽  
Functional Sites ◽  
Antibody Titers ◽  
Vitro Model ◽  
Von Willebrand ◽  
Willebrand Factor

Murine monoclonal antibodies to porcine Willebrand factor were used to study the role of Willebrand factor in hemostatic plug formation in the in vivo and in vitro skin bleeding times. The in vivo assay requires the intravenous injection of antibody-containing mouse ascitic fluids into normal animals with subsequent ear bleeding time tests performed over a 24-hour period. The in vitro assay was carried out with heparinized normal porcine blood flowing through a 0.5 cm incision in an excised piece of porcine skin. The heat inactivated ascitic fluids, containing the antibodies, were used ;Ln these assays at final dilutions of 2 × 102 through 2 × 105 The 7 antibodies used comprised at least 5 groups with differing reactivities based on assays other than bleeding time tests. The antibody titers in a poreineg Willebrand factor-binding radioimmunoassay ranged from 108 through 1012 . Doses of 10HL of ascitic fluid/kg of body weight (approximately 2 × 103 final dilution) were able to transiently prolong the in vivo bleeding time without alteration of other VIII complex values (VIII:C, ristocetin cofactor activity and Vlll-related antigen). Higher doses resulted in bleeding times similar to von Willebrand pigs (>15 min) immediately following infusion, with a decay of the effect over the next 2 hours. At dilutions of 2 × 104 selected monoclonal antibodies interfered with hemostasis in the in vitro model whereas other antibodies inhibited only at 2 × 102 dilution. With one exception, the potencies of the antibodies appeared to be similar in both assays. The titer of the antibodies in the radioimmunoassay does not appear to correlate with prolongation of the in vitro bleeding time, suggesting specific reactivity with functional sites involved in hemostatic plug formatio.

The Influence of Hydrophilic Decoration on X-ray Excited Luminescence Nanoparticles to Singlet Oxygen Production

NANO ◽  
2020 ◽  
Vol 15 (07) ◽  
pp. 2050092
Author(s):  
Xiaosheng Zhu ◽  
Yi Tian ◽  
Lei Dai ◽  
Qiaofeng Wang ◽  
Mei Shi ◽  
...  
Keyword(s):  
Quantum Yield ◽  
Exchange Reaction ◽  
X Ray ◽  
Good Biocompatibility ◽  
Place Exchange ◽  
High Concentrations ◽  
Excited Luminescence ◽  
Tumor Inhibition Rate

(1) Background: Though X-ray excited photodynamic therapy (X-PDT) breakthrough the bottom neck of PDT application in deep tumor by overcoming light penetration depth limitation, the quantum yield of the hydrophilic X-PDT nanoparticles (NPs) still hampered its further application in vivo. Thus, establishing a proper hydrophilic decoration method which can maximally maintain the quantum yield of X-ray excited luminescent NPs is of urgent demand. (2) Methods: We synthesized NaGdF4: [Formula: see text] (NGF) as X-ray excited luminescent NPs and conducted hydrophilic decoration by two hydrophilic ligands, polyethylene glycol-NH2 (PEG) and cysteamine (Cy) via place exchange reaction, and coupled with photosensitizer (MC540) to form a X-PDT nanosystem. We also conducted experiments in vitro and in vivo to evaluate the efficacy of the X-PDT system. (3) Results: Both PEG and Cy decoration NPs presented excellent emission intensity, which could well excite the coupled photosensitizer MC540 to generate significant X-PDT efficacy under low-dose X-ray radiation. Especially for the NGF-Cy-MC540 treatment group, the cell viability reduced to [Formula: see text]% under 0.3[Formula: see text]Gy radiation and [Formula: see text]% under only 0.1[Formula: see text]Gy radiation, which is the lowest radiation dosage in the literature reports so far. In vivo experiment showed about 36% of tumor inhibition rate under 0.3[Formula: see text]Gy X-ray. Besides, no biotoxicity was observed in NGF groups even in high concentrations, demonstrating good biocompatibility. (4) Conclusions: The hydrophilic decoration method by Cy or PEG via place exchange reaction may pave a brand new way and strategy for X-PDT further clinical application.

scholarly journals Hemostatic Dressings Made of Oxidized Bacterial Nanocellulose Membranes

2021 ◽  
Vol 2 (1) ◽  
pp. 80-99
Author(s):  
E. C. Queirós ◽  
S. P. Pinheiro ◽  
J. E. Pereira ◽  
J. Prada ◽  
I. Pires ◽  
...  
Keyword(s):  
Degree Of Polymerization ◽  
Oxidation Degree ◽  
Bacterial Nanocellulose ◽  
In Vivo Assays ◽  
Hemostatic Activity ◽  
Critical Issues ◽  
Good Biocompatibility ◽  
Tempo Radical

Surgicel® (regenerated oxidized cellulose) is a bio-absorbable hemostatic material widely applied to prevent surgery-derived adhesions. Some critical issues have been reported associated with this biomaterial, which we aimed to overcome by producing bacterial nanocellulose (BNC) membranes with hemostatic activity, through electrochemical oxidation using the tetramethylpiperidine-1-oxyl (TEMPO) radical. Samples were characterized by FTIR, NMR, SEM, XRD and their degree of polymerization. The oxidation degree was evaluated by titration of the carboxyl groups and the hemostatic behavior by whole-blood-clotting assays. In vitro and in vivo biodegradability of oxidized BNC membranes were evaluated and compared with that of Surgicel®. The oxidation degree increased from 4% to 7% and up to 15%, corresponding to an applied charge of 400, 700 and 1200 Coulombs, respectively. The oxidized BNC preserved the crystallinity and the 3D nano-fibrillar network, and demonstrated hemostatic activity, although not as effective as that of Surgicel®. In vivo assays demonstrated that the oxidized membranes did not induce an inflammatory response, revealing a good biocompatibility. However, non-degraded oxidized BNC was still detected at the implantation site after 56 days.

scholarly journals Novel Hydrogel Scaffolds Based on Alginate, Gelatin, 2-Hydroxyethyl Methacrylate, and Hydroxyapatite

Polymers ◽  
2021 ◽  
Vol 13 (6) ◽  
pp. 932
Author(s):  
Simonida Lj. Tomić ◽  
Jasmina Nikodinović-Runić ◽  
Marija Vukomanović ◽  
Marija M. Babić ◽  
Jovana S. Vuković
Keyword(s):  
The Novel ◽  
Hydroxyethyl Methacrylate ◽  
Healthy Human ◽  
Hydrogel Scaffolds ◽  
Polymeric Biomaterials ◽  
Porous Microstructure ◽  
Good Biocompatibility ◽  
New Generation

Hydrogel scaffolding biomaterials are one of the most attractive polymeric biomaterials for regenerative engineering and can be engineered into tissue mimetic scaffolds to support cell growth due to their similarity to the native extracellular matrix. The novel, versatile hydrogel scaffolds based on alginate, gelatin, 2-hydroxyethyl methacrylate, and inorganic agent hydroxyapatite were prepared by modified cryogelation. The chemical composition, morphology, porosity, mechanical properties, effects on cell viability, in vitro degradation, in vitro and in vivo biocompatibility were tested to correlate the material’s composition with the corresponding properties. Scaffolds showed an interconnected porous microstructure, satisfactory mechanical strength, favorable hydrophilicity, degradation, and suitable in vitro and in vivo biocompatible behavior. Materials showed good biocompatibility with healthy human fibroblast in cell culture, as well as in vivo with zebrafish assay, suggesting newly synthesized hydrogel scaffolds as a potential new generation of hydrogel scaffolding biomaterials with tunable properties for versatile biomedical applications and tissue regeneration.

scholarly journals Antimicrobial Effect and Cytotoxic Evaluation of Mg-Doped Hydroxyapatite Functionalized with Au-Nano Rods

Molecules ◽  
2021 ◽  
Vol 26 (4) ◽  
pp. 1099
Author(s):  
Domenico Franco ◽  
Giovanna Calabrese ◽  
Salvatore Petralia ◽  
Giulia Neri ◽  
Carmelo Corsaro ◽  
...  
Keyword(s):  
Bone Matrix ◽  
Antibacterial Properties ◽  
Antimicrobial Effect ◽  
Cell Number ◽  
Bacterial Reduction ◽  
E Coli ◽  
Good Biocompatibility ◽  
Inorganic Mineral

Hydroxyapatite (HA) is the main inorganic mineral that constitutes bone matrix and represents the most used biomaterial for bone regeneration. Over the years, it has been demonstrated that HA exhibits good biocompatibility, osteoconductivity, and osteoinductivity both in vitro and in vivo, and can be prepared by synthetic and natural sources via easy fabrication strategies. However, its low antibacterial property and its fragile nature restricts its usage for bone graft applications. In this study we functionalized a MgHA scaffold with gold nanorods (AuNRs) and evaluated its antibacterial effect against S. aureus and E. coli in both suspension and adhesion and its cytotoxicity over time (1 to 24 days). Results show that the AuNRs nano-functionalization improves the antibacterial activity with 100% bacterial reduction after 24 h. The toxicity study, however, indicates a 4.38-fold cell number decrease at 24 days. Although further optimization on nano-functionalization process are needed for cytotoxicity, these data indicated that Au-NRs nano-functionalization is a very promising method for improving the antibacterial properties of HA.

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