scholarly journals Efficacy of Aloe Vera Hydrogel in Inhibition The Growth of Campylobacter rectus and Provetella intermedia Bacteria

2021 ◽  
Vol 5 (8) ◽  
pp. 784-790
Author(s):  
Chandra Susanto ◽  
Member Reni Purba ◽  
Ridha Mahrani ◽  
Ramayani Efendi
Keyword(s):  
Experimental Study ◽  
Side Effects ◽  
Aloe Vera ◽  
Positive Control ◽  
Control Group ◽  
Control Group Design ◽  
Group Design ◽  
Campylobacter Rectus ◽  
Group Data

Introduction: Campylobacter rectus and Provetella intermedia bacteria as the main etiology causes of periodontal disease. One of the locally synthesized antimicrobials that is widely used for the treatment of periodontitis is Chlorhexidine, however this drug has side effects when used continuously. To overcome this, an alternative periodontitis therapy with medicinal plants is needed. This study aims to determine the effectiveness of hydrogel Aloe vera antibacterial concentrations of 2.5%, 5%, 10% and 20% in the bacteria Campylobacter rectus and Provetella intermedia. Methods: This study was a laboratory experimental study with posttest only control group design in vitro. The samples were Campylobacter rectus and Provetella intermedia. The number of groups in this study was six. The sample size was determined by Federer's formula and obtained four replications for each group. Data collection by measuring the inhibition diameter using a slide caliper. Data were analyzed using oneway ANOVA and posthoc LSD. Results: The results showed that there was a difference in the diameter of the hydrogel Aloe vera inhibition with a concentration of 2.5%, 5%, 10%, 20% and a positive control for Campylobacter rectus and Provetella intermedia bacteria. Conclusion: This study concluded that there is an antibacterial effectiveness of hydrogel Aloe vera in concentrations of 2.5%, 5%, 10% and 20% on Campylobacter rectus and Provetella intermedia bacteria.  

scholarly journals Efficacy of Aloe Vera Hydrogel in Inhibition The Growth of Campylobacter rectus and Provetella intermedia Bacteria

2021 ◽  
Vol 5 (3) ◽  
pp. 783-789
Author(s):  
Chandra Susanto ◽  
Member Reni Purba ◽  
Ridha Mahrani ◽  
Ramayani Efendi
Keyword(s):  
Experimental Study ◽  
Side Effects ◽  
Aloe Vera ◽  
Positive Control ◽  
Control Group ◽  
Control Group Design ◽  
Group Design ◽  
Campylobacter Rectus ◽  
Group Data

Introduction: Campylobacter rectus and Provetella intermedia bacteria as the main etiology causes of periodontal disease. One of the locally synthesized antimicrobials that is widely used for the treatment of periodontitis is Chlorhexidine, however this drug has side effects when used continuously. To overcome this, an alternative periodontitis therapy with medicinal plants is needed. This study aims to determine the effectiveness of hydrogel Aloe vera antibacterial concentrations of 2.5%, 5%, 10% and 20% in the bacteria Campylobacter rectus and Provetella intermedia. Methods: This study was a laboratory experimental study with posttest only control group design in vitro. The samples were Campylobacter rectus and Provetella intermedia. The number of groups in this study was six. The sample size was determined by Federer's formula and obtained four replications for each group. Data collection by measuring the inhibition diameter using a slide caliper. Data were analyzed using oneway ANOVA and posthoc LSD. Results: The results showed that there was a difference in the diameter of the hydrogel Aloe vera inhibition with a concentration of 2.5%, 5%, 10%, 20% and a positive control for Campylobacter rectus and Provetella intermedia bacteria. Conclusion: This study concluded that there is an antibacterial effectiveness of hydrogel Aloe vera in concentrations of 2.5%, 5%, 10% and 20% on Campylobacter rectus and Provetella intermedia bacteria.  

scholarly journals Uji Daya Hambat Ekstrak Ikan Nike (Awous melanocephalus) Terhadap Pertumbuhan Bakteri Fusobacterium nucleatum

e-GIGI ◽  
2021 ◽  
Vol 9 (2) ◽  
pp. 238
Author(s):  
Stevia E. Nonutu ◽  
Damajanty H. C. Pangemanan ◽  
Christy N. Mintjelungan
Keyword(s):  
Side Effects ◽  
Treatment Options ◽  
Antibacterial Properties ◽  
Fusobacterium Nucleatum ◽  
Inhibitory Effect ◽  
Positive Control ◽  
Negative Control ◽  
Control Group ◽  
Control Group Design ◽  
Group Design

Abstract: One of the treatment options of periodontal abscess caused by Fusobacterium nucleatum is administration of antibiotics. However, long-term antibiotics consumption can cause negative side effects. Therefore, alternative treatments that have low side effects and easy to be obtained are needed. Nike fish (Awaous melanocephalus) is one of the endemic fish of North Sulawesi province which has antibacterial properties. This study was aimed to evaluate the inhibition effect of nike fish extract on the growth of Fusobacterium nucleatum. This was a true experimental study with a posttest only control group design. We used modified Kirby-Bauer method with filter papers. Ciprofloxacin was used as the positive control and aquadest as the negative control. Extract of nike fish and stock of pure bacteria Fusobacterium nucleatum were prepared. The results showed that the average diameters of the inhibition zones formed in the nike fish extract after three repetitions, were as follows: for extract concentration of 12.5% was 2.91 mm; 25% was 4.16 mm; 50% was 8.41 mm; and 100% was 9.58 mm. In conclusion, nike fish extract (Awaous melanocephalus) at concentrations of 50% and 100% had a weak inhibitory effect (Himedia category) on the growth of Fusobacterium nucleatum meanwhile at concentrations of 12.5% and 25% there was no activity of zone of inhibition.Keywords: extract of nike fish (Awaous melanocephalus); Fusobacterium nucleatum; inhibitory effect Abstrak: Salah satu opsi pengobatan abses periodontal yang disebabkan oleh bakteri Fusobacterium nucleatum yaitu dengan penggunaan antibiotik namun mengonsumsi antibiotik jangka panjang dapat menimbulkan efek samping negatif. Oleh karena itu, diperlukan pengobatan alternatif yang memiliki efek samping rendah serta mudah didapat. Ikan nike merupakan salah satu ikan endemik Provinsi Sulawesi Utara yang berkhasiat sebagai antibakteri. Penelitian ini bertujuan untuk mengetahui daya hambat ekstrak ikan nike (Awaous melanocephalus) terhadap pertumbuhan bakteri Fusobacterium nucleatum. Jenis penelitian ialah eksperimental murni dengan post test only control group design. Metode yang digunakan yaitu metode modifikasi Kirby-Bauer dengan menggunakan paper disk. Kontrol positif menggunakan antibakteri ciprofloxacin dan kontrol negatif menggunakan akuades. Pada penelitian ini digunakan ekstrak ikan nike dan stok bakteri murni Fusobacterium nucleatum. Hasil penelitian menunjukkan bahwa rerata diameter zona hambat yang terbentuk pada ekstrak ikan nike setelah tiga kali pengulangan yaitu untuk konsentrasi 12,5% sebesar 2,91 mm; 25% sebesar 4,16 mm; 50% sebesar 8,41 mm; dan 100% sebesar 9,58 mm. Simpulan penelitian ini ialah ekstrak ikan nike (Awaous melanocephalus) pada konsentrasi 50% dan 100% memiliki daya hambat kategori lemah (Himedia) terhadap pertumbuhan bakteri Fusobacterium nucleatum sedangkan pada konsentrasi 12,5% dan 25% dikategorikan tidak terdapat aktivitas zona hambat. Kata kunci: ekstrak ikan nike (Awaous melanocephalus); Fusobacterium nucleatum; daya hambat

scholarly journals DAYA HAMBAT EKSTRAK LIDAH BUAYA (Aloe barbadensis Miller) TERHADAP PERTUMBUHAN BAKTERI Staphylococcus aureus SECARA IN VITRO

2019 ◽  
Vol 10 (01) ◽  
pp. 23-32
Author(s):  
Pramesti Indah Prabasari ◽  
I M Sumarya ◽  
N.K.A. Juliasih
Keyword(s):  
Staphylococcus Aureus ◽  
Aloe Vera ◽  
Inhibition Zone ◽  
Inhibitory Effect ◽  
Positive Control ◽  
Control Group ◽  
Aloe Barbadensis ◽  
Control Group Design ◽  
Inhibitory Power

Aloe vera (Aloe barbadensis Miller) contains anthraquinone compounds, flavonoids and saponins which are thought to have antibacterial effects so as to inhibit bacterial growth. The purpose of the study was to determine the inhibitory power of Aloe barbadensis Miller on the growth of Staphylococcus aureus bacteria. The pure experimental study was conducted with The Randomized Posttest Only Control Group Design using six (6) treatments, namely aloe vera extract with a concentration of 25%, 50%, 75%, 100%, Chloramphenicol 30 mcg antibiotics as a positive control, and sterile aquadest as a control negative for Staphylococcus aureus bacteria grown on Mueller Hinton media. After being treated, it was incubated for 24 hours and measured its inhibitory power. The growth inhibition data of Staphylococcus aureus bacteria in the form of inhibition zone diameter was analyzed statistically by Kruskal Wallis test, at the level of confidence α = 0.05. The results showed that the average inhibitory effect of Aloe vera extract on the growth of Staphylococcus aureus bacteria in concentrations of 25%, 50%, 75% and 100% were 0 ± 0 mm; 7.125 ± 0.1250 mm; 8.375 ± 0.23936 mm; and 9.0 ± 0.40825 mm. Based on the results of the study concluded that aloe vera extract can inhibit the growth of Staphylococcus aureus bacteria. The higher the concentration of Aloe vera extract the greater the inhibitory effect on the growth of Staphylococcus aureus bacteria.

scholarly journals AKTIVITAS LARVASIDA EKSTRAK ETANOL DAUN BINJAI (Mangifera caesia) TERHADAP LARVA Aedes aegypti

2017 ◽  
Vol 13 (1) ◽  
pp. 61
Author(s):  
Isfarani Nadila ◽  
Istiana Istiana ◽  
Erida Wydiamala
Keyword(s):  
Experimental Study ◽  
Aedes Aegypti ◽  
Ethanol Extract ◽  
Preliminary Test ◽  
Positive Control ◽  
Control Group ◽  
Control Group Design ◽  
Group Design ◽  
Lc50 Value ◽  
Significant Difference

Abstract: Binjai leaf is known to have effective secondary metabolites as larvacide. The aim of this study was to recognize larvacidal effectivity of Binjai leaf ethanol extract toward Aedes aegypti larva. This was experimental study with posttest only control group design by using 7 treatment and 4 replication. Treatment was available from preliminary test which were 1000, 5000, 10.000, 15.000 and 20.000 (mg/L), negative xontrol (water) and positive control (themephos 100 mg/L). Probit test resulted in LC50 value=5493.390 mg/L and LC90 value=14988.861mg/L Kruskal-Wallis test resulted in p=0,000 which meaned there was an effect of binjai leaf ethanol extract toward Aedes aegypti larva. Meanwhile, Mann-Whitney test resulted in p=0,371 which meaned there was no significant difference between concentration of 20.000 mg/L and temephos 100 mg/L. In conclusion, binjai leaf (Mangifera caesia) ethanol extract have larvacidal activity as effective as temephos toward Aedes aegypti larva. Keywords: Larvacide, Binjai leaves, Aedes aegypti Abstrak: Daun binjai diketahui memiliki kandungan metabolit sekunder yang berpotensi sebagai larvasida. Tujuan penelitian ini adalah untuk mengetahui efektivitas larvasida ekstrak etanol daun binjai terhadap larva Aedes aegypti. Penelitian ini menggunakan jenis eksperimental dengan metode posttest only contol grup design menggunakan tujuh kelompok perlakuan dan 4 replikasi. Tujuh kelompok perlakuan tersebut adalah 5 serial konsentrasi yang diperoleh dari uji pendahuluan: 1000, 5000, 10.000, 15.000 dan 20.000 (mg/L), kontrol negatif (air) dan kontrol positif (temephos 100 mg/L). Hasil uji probit didapatkan  nilai LC50 dan LC90 sebesar 5493.390 dan 14988.861 (mg/L). Uji kruskal-Wallis didapatkan  nilai p=0,000, terdapat pengaruh ekstrak etanol daun binjai terhadap larva Aedes aegypti. Hasil uji Mann-whitney didapakan nilai  p=0,371, tidak terdapat perbedaan signifikan antara konsentrasi 20.000 mg/L dengan kontrol positif. Kesimpulan dari penelitian ini ekstrak etanol daun binjai memiliki aktivitas larvasida dan efektivitas setara dengan temephos 100 mg/L terhadap larva Aedes aegypti. Kata-kata kunci :  Larvasida, daun binjai, Aedes aegypti

scholarly journals Antibacterial Efficacy of Aloe vera Sap Against Staphylococcus aureus and Escherichia coli

2019 ◽  
Vol 3 (2) ◽  
pp. 29-37
Author(s):  
Safira Azahra ◽  
Nita Parisa ◽  
Fatmawati Fatmawati ◽  
Ella Amalia ◽  
Venny Larasati
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Aloe Vera ◽  
Control Group ◽  
Antibacterial Efficacy ◽  
Control Group Design ◽  
Group Design ◽  
Post Test ◽  
Alternative Drug

Abstract Background Aloe vera is a plant that has been used as an alternative drug. This plant contains various compounds, like anthraquinone, saponin, flavonoid, alkaloid, and tannin that has an antibacterial effect against Staphylococcus aureus and Escherichia coli. Both of it were responsible for the infection incident. This study aims to determine the efficacy of Aloe vera sap as an antibacterial against Staphylococcus aureus and Escherichia coli. Methods An experimental study, in vitro using post-test only control group design, has been done at laboratory of Medical Faculty of Sriwijaya University, by examining the antibacterial activity of Aloe vera sap in five different concentration (5%, 10%, 20%, 40%, dan 80%) using well diffusion and solid dillusion method to determine the minimum bactericidal concentration (MBC). And then continued with the phytocemical screening to determine the compound inside the Aloe vera sap. Results Aloe vera sap were able to kill Staphylococcus aureus at 5% and Eshcerichia coli at 80%. Compatibility test showed that Aloe vera sap with concentration of 10%, 20%, 40%, and 80% are compatible with amoxicillin, therefore 80% is compatible with cefotaxime. This ability due to the compound that it contains, which is alkaloid, flavonoid, tannin, quinone, and saponin. Conclusion Aloe vera sap is effective as an antibacterial against Staphylococcus aureus and Escherichia coli.   Keyword: Aloe vera sap, antibacterial, efficacy, Staphylococcus aureus, Escherichia coli.  

PENGARUH SUHU PEMANASAN FORMALIN 10% TERHADAP PERKEMBANGAN TELUR Ascaris lumbricoides

2018 ◽  
Vol 6 (1) ◽  
Author(s):  
Ni Putu Aryadnyani
Keyword(s):  
Experimental Study ◽  
Environmental Factors ◽  
Ascaris Lumbricoides ◽  
Heating Temperature ◽  
Control Group ◽  
Control Group Design ◽  
Group Design ◽  
Design Result

AbstractBackground Ascaris lumbricoides eggs had very thick walls consisted of three layers, they were albuminoid layer, hyaline layer and vitelin layer. These layers were impermeable causing the Ascaris lumbricoides eggs resistant to less supportive environmental factors. Formalin 10% was a preservative that was often used to preserve faeces containing parasites such as protozoa and worm eggs. However, without heating, formalin 10% was not effective to preserve the Ascaris lumbricoides eggs because they would keep developing to become infective (containing larvae). Objective This study aims to prove whether there is an effect of adding 10% formalin which is heated at 60 C, 70 C and 80 C to the development of Ascaris lumbricoides eggs.Methods The design of this research was an experimental study with The Randomized Posttest Control Group Design. Result The Ascaris lumbricoides eggs were still growing into infective eggs in faeces although they were heated by formalin 10% at 60C, 70C and 80C. conclusion Based on the results of the study, there was no effect of heating temperature of formalin 10% on the development of Ascaris lumbricoides egg.Keywords: Formalin 10%, Ascaris lumbricoides, Heating, Soil Transmitted Helminth

PENGELUARAN KOLOSTRUM DENGAN PEMBERIAN PERAWATAN PAYUDARA DAN ENDORPHIN MASSAGE PADA IBU POST SEKSIO SESAREA

2019 ◽  
Vol 2 (1) ◽  
pp. 34-40
Author(s):  
Diah Evawanna Anuhgera ◽  
Eka Fitria Panjaitan ◽  
Desika Wali Pardede ◽  
Desika Wali Pardede ◽  
Nikmah Jalilah Ritonga ◽  
...  
Keyword(s):  
Experimental Study ◽  
Cesarean Section ◽  
Alternative Therapy ◽  
Control Group ◽  
Control Group Design ◽  
Group Design ◽  
A Value ◽  
Quasi Experimental ◽  
Breast Care ◽  
June July

Spending colostrum in post-cesarean section mothers is difficult in the first week postpartum. Efforts to overcome colostrum disruption can use non-pharmacological methods, namely breast care and endorphin massage. This study aims to determine the expenditure of colostrum by giving breast care and endorphin massage to post-cesarean mothers. This research is a quasi-experimental study with a nonequivalent design posttest control group design. This research was carried out in the midwifery room of the second floor of Grand Medistra Lubuk Pakam Hospital during the June-July 2019 period. There were 48 participants selected using purposive sampling, with 18 assigned in breast care, endorphin massage and combination of breast care and endorphin massage. The time of spending colostrum expenditure is assessed every day while the amount of colostrum expenditure is assessed after intervening. Analysis of the data used is one-way Annova. The results showed that the combination of breast care and endorphin massage was the most effective action in the time of spending colostrum and the amount of colostrum with a value of p = 0,000. The combination of breast care and endorphin massage could be applied as an alternative therapy post-cesarean mothers    

scholarly journals Uji Daya Hambat Air Perasan Buah Jeruk Nipis (Citrus aurantifolia s.) Terhadap Pertumbuhan Bakteri Staphylococcus Aureus Secara In Vitro

2013 ◽  
Vol 2 (1) ◽  
pp. 05 ◽  
Author(s):  
Abdul Razak ◽  
Aziz Djamal ◽  
Gusti Revilla
Keyword(s):  
Staphylococcus Aureus ◽  
Wound Healing ◽  
Contact Time ◽  
Experimental Methods ◽  
Control Group ◽  
Control Group Design ◽  
Citrus Aurantifolia ◽  
Group Design ◽  
Growth Of Bacteria

AbstrakJeruk Nipis (Citrus aurantifolia S.) merupakan salah satu tanaman obat keluarga yang banyak terdapat ditengah masyarkat dan banyak digunakan sebagai ramuan tradisional. Bagian yang sering digunakan adalah air perasannya, dengan salah satu manfaat dapat digunakan untuk menghilangkan jerawat serta penyembuhan luka agar tidak terjadi abses. Jerawat dan abses pada luka merupakan salah satu infeksi yang disebabkan oleh bakteri Staphylococcus aureus.Tujuan Penelitian ini adalah untuk mengetahui daya hambat air perasan buah jeruk nipis (Citrus aurantifolia S.) terhadap pertumbuhan bakteri Staphylococcus aureus secara invitro. Penelitian dilakukan dengan metoda eksperimental laboratorium dengan desain postest only control group design yang dilakukan di Laboratorium Mikrobiologi Fakultas Kedokteran Universitas Andalas.Hasil penelitian menunjukan bahwa air perasan buah jeruk nipis memiliki daya hambat terhadap pertumbuhan bakteri Staphylococcus aureus dengan berbagai konsentrasi yaitu 25%, 50%, 75%, dan 100% dan terdapat pengaruh lama kontak terhadap pertumbuhan bakteri dimana bakteri tidak tumbuh seteleh kontak 5 menit pertama dan diikuti menit-menit berikutnya dengan air perasan buah jeruk nipis konsentrasi 100%. Jadi, semakin tinggi konsentrasi air perasan buah jeruk nipis dan semakin lama kontak dengan bakteri Staphylococcus aureus maka daya hambatnya semakin baik.Kata kunci: Uji Daya Hambat, Air Perasan Buah Jeruk Nipis, Staphylococcus aureus.Abstract Lime (Citrus aurantifolia S.) is kind of family’s herbal medicine, most using in the community is widely used as a traditional herb. The most common used part is the lime fruit squeeze with one of the function is used for removing acne and wound healing to prevent the form of abscess. Pimples and abscesses of the wound is one of the infections caused by the bacterium Staphylococcus aureus.The purpose of this study was to determine the inhibition of lime fruit (Citrus aurantifolia S.) squeeze towards the growth of the bacteria Staphylococcus aureus in vitro condition. The study was conducted with laboratory experimental methods to the design of control group design postest only performed at the Laboratory of Microbiology Faculty of Medicine, University of Andalas.The results showed that the lime fruit (Citrus aurantifolia S.) squeeze has the ability to inhibite the bacterial growth of Staphylococcus aureus with various concentrations of 25%, 50%, 75%, and 100% and there is the effect of contact time on the growth of bacteria which the bacteria do not grow after contact the first 5 minutes and the next minute followed by lime fruit squeeze with 100% concentration lime fruit squeeze. Thus, the higher the concentration of lime fruit squeeze and the longer the contact with the bacteria Staphylococcus aureus is the better towards.Keywords:Inhibition test, The Lime Fruit Squeeze, Staphylococcus Aureus.

scholarly journals Optimization of Learning Through Edmodo-Based Hybrid Learning to Improve Learning Independence and Learning Outcomes

2020 ◽  
Vol 23 (1) ◽  
pp. 61
Author(s):  
Umi Kulsum
Keyword(s):  
Learning Outcomes ◽  
Hybrid Learning ◽  
Control Group ◽  
Control Group Design ◽  
Face To Face ◽  
Group Design ◽  
Media Study ◽  
Group Data ◽  
Equivalent Control ◽  
Relationship Of

The study aims to determine the effectiveness of the use of Edmodo as a media study review of the learning outcomes; Knowing the learning independence relationship with learning outcomes and Knowing the influence of hybrid learning on learning independence and learning outcomes. This is experimental method with  Non-Equivalent  Control  Group Design,  consists of four groups of treatment with varied hybrid learning proportions,  each  50% (meaning  50%  learning hybrid learning and  50%  face to face), 60% (meaning  60%  of hybrid learning and  40%  face to face),  and  70% (meaning  70%  hybrid learning and  30%  face-to-face),  and one  group of others is a  conventional group (only face to face),  this group as a  control group. Data Collection with questionnaires for learning independence and knowledge tests to determine learning achievement. The  results  showed: (1)  the  use  of  Edmodo  was  effective  in  enhancing  the  results  of  a  larger  experimental  class  learning  compared  to  the  control  class  so  that  learning  became  Optimal, (2) a  significant  relationship  of learning independence learning  with  learning  outcomes,  significance  0.000, (3)  there  is  a  difference  of  influence  of  significant  variation  of  hybrid  learning  to learning independence and  learning  outcomes,  with  significance  0,037

scholarly journals Efektivitas Metode Imla’ Manzur dalam Meningkatkan Keterampilan Menulis Bahasa Arab Siswa Madrasah Tsanawiyah

2020 ◽  
Vol 1 (2) ◽  
pp. 1-22
Author(s):  
Susanti Susanti
Keyword(s):  
Experimental Study ◽  
Data Collection ◽  
Learning Outcomes ◽  
Writing Skills ◽  
T Test ◽  
Control Group ◽  
Control Group Design ◽  
Group Design ◽  
Average Value ◽  
Significant Difference

The lack of use the writing skills method raises several obstacles experienced by students, including weaknesses in understanding the basics of Arabic writing and difficulty in answering Arabic questions. Therefore, it is necessary to improve learning as an effort to improve writing skills by applying the imla’ manzur method. This research is an experimental study with a pretest-posttest control group design model, aimed at finding out the effectiveness of the imla’ manzur method. Data collection techniques in this study used observation, tests, questionnaires, interviews, and documentation. Then the validity of the data is tested using the independent sample t-test. The results obtained were that in the experimental class the mastery of Arabic writing skills of students after applying the imla’ manzur method had increased the average value from 52.08 to 78.57. Then, the results of the independent sample t-test showed a significant difference in the mastery of Arabic writing skills in the experimental class and the control class 0.000 < 0.05 after treatment, so that the conclusion of this study based on the data obtained showed that the application of the imla’ manzur method was effective in improving students Arabic writing skills. Evidenced by a significant increase in learning outcomes, student enthusiasm when taking turns to write vocabulary and sentences in Arabic on the board, as well as growing sensitivity of student analysis of Arabic writing skills.

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