PECULIARITIES OF FORMATION OF THE LOWER CRETACEOUS TEСTONO-SEDIMENTATION SYSTEM OF THE BLACK SEA MEGADEPRESSION

Based on the analysis of the distribution of structural-material complexes, facial-genetic types and modeling of the history of immersion of the Lower Cretaceous sediments, the spatial-temporal differentiation of the tectonic regime of the Black Sea megadepression in the Early Cretaceous was established. It is shown that the structural and lithologicalfacial features of the Lower Cretaceous tectonic-sedimentation system were determined by the interaction of tectonic and geological-paleooceanographic factors. Thus, the low sea level and the initial phases of activation of sublatitudinal faults in the Hoterivian-Aptian determined the dominant influence of submeridional tectonic faults on the formation of the corresponding structural-facial block zonation. The increase in the area of the basin, the removal of denudation areas in the Late Albian reduced the intensity of terrigenous discharge, and the prolonged activity of sublatitudinal faults led to the formation of sublatitudinal structural-facial zonation. In the subsequent geological history, the tectonic regime was characterized by a complex mosaic-block character, which influenced the heterogeneity of oil and gas lithofluid systems of Cretaceous deposits.

Mineralogical features of gold of the Permian conglomerates of the Sololiy uplift of the Olenek high (Northeastern Siberian platform)

Typomorphic features of placer gold of conglomerates of the Ortokinskaya formation of the Early Permian age (P1or) of the Sololiy Uplift of the Olenek High and modern channel alluvium of the Ortoku Eekit river, which drains these deposits, are studied. It is found that extremely heterogeneous composition and complex inner structure are typical features of the vast majority of gold of the Permian conglomerates. There are several stages of different fineness (from 251‰ to 999‰) within a single gold particle, as a result, gold has a mosaic-block inner structure. It is proposed that such heterogeneity is formed due to epigenetic transformations of primary medium-grade gold in an intermediate reservoir of the Permian age, and also under influence of superposed hydrothermal mineralization with low-grade gold, related to the processes of the Mesozoic tectonomagmatic activation. The latter is indicated by the discovery of impregnated gold-sulfide mineralization in the Permian conglomerates. Ore mineralization is represented by gold, silver, pyrite, sphalerite, chalcopyrite, galena, argentite, native tin, and barite. Association mineralization to thin tectonic zones of conglomerate fragmentation, suggests that superposed gold-sulfide hydrothermal mineralization of impregnated type was formed as a result of manifestations of the Mesozoic tectonomagmatic activation, when gold-bearing hydrothermal solutions migrated in faults. It is determined that auriferous conglomerates of the Ortokinskaya formation of the Permian age were major sources of placer gold of modern alluvium in the studied part of the Ortoku Eekit.

Innervation Anomalies and Interneural Anastomoses: Is There Clinical Relevance or Not?

Failed block could be one of the adverse results of the conductive anaesthesia. There are situations, when in seemingly perfect technique of the block, properly chosen drug, dosage, adjuvant – the block is not achieved (it does not occur or is incomplete or so-called “mosaic block” occurs). It is necessary to remember about atypical or abnormal nerves branching, possible interneural anastomoses, when looking for the possible cause of it. Information about most common types of interneural anastomosis of arm’s and leg’s long nerves is presented in this article. Knowledge of possible abnormal neural innervation can provide an understanding of inefficacy of some simple blocks, decrease amount of mistakes in data interpretation, that was obtained during electroneuromyographic (ENMG) investigations, improve understanding of clinical pattern in the peripheral nerve injuries, that form anastomosis.

Intelligibility of English Mosaic Speech: Comparison between Native and Non-Native Speakers of English

Mosaic speech is degraded speech that is segmented into time × frequency blocks. Earlier research with Japanese mosaic speech has shown that its intelligibility is almost perfect for mosaic block durations (MBD) up to 40 ms. The purpose of the present study was to investigate the intelligibility of English mosaic speech, and whether its intelligibility would vary if it was compressed in time, preserved, or stretched in time. Furthermore, we investigated whether intelligibility differed between native and non-native speakers of English. English (n = 19), Indonesian (n = 19), and Chinese (n = 20) listeners participated in an experiment, in which the mosaic speech stimuli were presented, and they had to type what they had heard. The results showed that compressing or stretching the English mosaic speech resulted in similar trends in intelligibility among the three language groups, with some exceptions. Generally, the intelligibility for MBDs of 20 and 40 ms after preserving/stretching was higher, and decreased beyond MBDs of 80 ms after stretching. Compression also lowered intelligibility. This suggests that humans can extract new information from individual speech segments of about 40 ms, but that there is a limit to the amount of linguistic information that can be conveyed within a block of about 40 ms or below.

4165 Mechanisms of Prophage-Mediated Virulence Driving Community-Acquired MRSA Contagion

OBJECTIVES/GOALS: We recently identified a CA-MRSA strain in Brooklyn, New York (USA300-BKV) causing an outbreak of severe skin infections in predominantly healthy children. The evolution of USA300-BKV included acquisition of a novel prophage, and our objective is to identify the prophage-encoded gene(s) and mechanism responsible for increased bacterial virulence. METHODS/STUDY POPULATION: We deleted candidate genes from a novel mosaic block of phage-encoded genes in USA300-BKV that have been shown to enhance virulence in a murine skin infection model. Deletion mutants and complemented clones will be evaluated in vivo to identify culprit genes and determine the effect of lineage-specific genetic variation on the phenotype. Complementary studies include a comprehensive characterization of phage and bacterial genes expressed during lysogeny in vitro using RNA sequencing (RNA-Seq), and in vivo using a targeted approach focusing on known bacterial virulence and phage lytic pathways as well as candidate genes identified by in vitro studies. RESULTS/ANTICIPATED RESULTS: Comparison of otherwise isogenic lab strains showed that the mosaic block of phage genes present in USA300-BKV enhance skin abscess size in mice, confirming previous results. As this region of the phage, named mΦ11, does not contain known toxin genes, we hypothesize that mΦ11 modulates expression of bacterial host genes to enhance virulence. Thus, transcriptional profiles of CA-MRSA containing mΦ11 and selected deletion mutants are expected to reveal changes in known or novel virulence factors compared to controls. Candidate regulators specific to the mosaic block include an adenine methyltransferase linked to changes in global gene expression of other bacterial species. DISCUSSION/SIGNIFICANCE OF IMPACT: Our results will broaden scientific understanding of phage-bacterial interactions and determine the mechanisms by which phage impact virulence independent from toxin gene carriage. Identification of phage-encoded gene(s) enhancing CA-MRSA contagion will inform surveillance efforts and identify novel therapeutic targets.

Diversity of Mosaic pbp2x Families in Penicillin-Resistant Streptococcus pneumoniae from Iran and Romania

ABSTRACT Penicillin-resistant Streptococcus pneumoniae strains are found at high rates in Romania and Iran. The mosaic structure of PBP2x was investigated in 9 strains from Iran and in 15 strains from Romania to understand their evolutionary history. Mutations potentially important for β-lactam resistance were identified by comparison of the PBP2x sequences with the sequence of the related PBP2x of reference penicillin-sensitive S. mitis strains. Two main PBP2x mosaic gene families were recognized. Eight Iranian strains expressed PBP2x variants in group 1, which had a mosaic block highly related to PBP2x of the Spain23F-1 clone, which is widespread among international penicillin-resistant S. pneumoniae clones. A second unique PBP2x group was observed in Romanian strains; furthermore, three PBP2x single mosaic variants were found. Sequence blocks of penicillin-sensitive strain S. mitis 658 were common among PBP2x variants from strains from both countries. Each PBP2x group contained specific signature mutations within the transpeptidase domain, documenting the existence of distinct mutational pathways for the development of penicillin resistance.

Matching X-ray beam and detector properties to protein crystals of different perfection

An analysis is given of the effect of different beam and detector parameters on the sharpness of recorded diffraction features for macromolecular crystals of different quality. The crystal quality parameters include crystal strain, crystal or mosaic block size and mosaic block misorientation. Calculations are given for instrument parameters such as angular resolution of the detector, beam divergence and wavelength bandpass to be matched to the intrinsic diffraction properties from these crystals with the aim of obtaining the best possible data out of each crystal. Examples are given using typical crystal imperfections obtained from the literature for both room-temperature and cryo-cooled crystals. Possible implications for the choice of X-ray source, beamline design, detector specifications, instrument set-up and data processing are discussed, together with the limitations of the approach.

A New Design of Digital Camouflage Spray Device

The digital camouflage technology is a new camouflage technique of military engineering. It sprays the Mosaic block of different color on the surface after permutation and combination according to certain rules. But in present, the current technology still takes artificial spraying method. This method cant adapt the present need of camouflage technique for military engineering whether spraying effect or spraying efficiency. For this reason, an intelligentized digital camouflage spraying device of cartesian coordinate robot is designed. The study could supply important references for theory research and project implementation of military digital camouflage technology.