Maize Brittle Stalk2-Like3, encoding a COBRA protein, functions in cell wall formation and carbohydrate partitioning

Carbohydrate partitioning from leaves to sink tissues is essential for plant growth and development. The maize (Zea mays) recessive carbohydrate partitioning defective28 (cpd28) and cpd47 mutants exhibit leaf chlorosis and accumulation of starch and soluble sugars. Transport studies with 14C-sucrose (Suc) found drastically decreased export from mature leaves in cpd28 and cpd47 mutants relative to wild-type siblings. Consistent with decreased Suc export, cpd28 mutants exhibited decreased phloem pressure in mature leaves, and altered phloem cell wall ultrastructure in immature and mature leaves. We identified the causative mutations in the Brittle Stalk2-Like3 (BK2L3) gene, a member of the COBRA family, which is involved in cell wall development across angiosperms. None of the previously characterized COBRA genes are reported to affect carbohydrate export. Consistent with other characterized COBRA members, the BK2L3 protein localized to the plasma membrane, and the mutants condition a dwarf phenotype in dark-grown shoots and primary roots, as well as the loss of anisotropic cell elongation in the root elongation zone. Likewise, both mutants exhibit a significant cellulose deficiency in mature leaves. Therefore, BK2L3 functions in tissue growth and cell wall development, and this work elucidates a unique connection between cellulose deposition in the phloem and whole-plant carbohydrate partitioning.

Thermomagnesium: A By-Product of Ni Ore Mining as a Clean Fertilizer Source for Maize

This study explores whether Thermomagnesium (TM), a by-product of Ni ore mining, is an efficient fertilizer for maize. The effects of TM on soil pH, the supply of Si and Mg to the soil and plants, carbohydrate metabolism, grain filling, and yield were assessed in two simultaneous experiments performed in greenhouse conditions. Five TM doses were applied to two soil textures—clayey (0, 55, 273, 709, and 2018 mg kg−1) and sandy (0, 293, 410, 645, and 1260 mg kg−1). In general, the best results in soil and maize plants occurred at the highest TM dose for both soil textures (clayey 2018 mg kg−1 and sandy 1260 mg kg−1). The results demonstrated that in both soils, the concentrations of Mg and Si in the maize leaves increased with the dose of TM, similarly to that which occurred in the soil. Interestingly, in clayey soil, the soil pH increased linearly, whereas in sandy soil, the pH reached its maximum value between the two largest TM doses. The concentration of reducing sugars increased at the highest TM dose, whereas the concentrations of sucrose and starch decreased. The enhancement of carbohydrate partitioning led to higher maize growth, grain filling, and yield. Overall, the results clearly demonstrate that TM is a sustainable alternative fertilizer for maize and can be used for countless other crops and soil classifications, thus providing a suitable destination for this by-product of Ni ore mining.

Evolutionary gain of oligosaccharide hydrolysis and sugar transport enhanced carbohydrate partitioning in sweet watermelon fruits

How raffinose (Raf) family oligosaccharides, the major translocated sugars in the vascular bundle in cucurbits, are hydrolyzed and subsequently partitioned has not been fully elucidated. By performing reciprocal grafting of watermelon (Citrullus lanatus) fruits to branch stems, we observed that Raf was hydrolyzed in the fruit of cultivar watermelons but was backlogged in the fruit of wild ancestor species. Through a genome-wide association study, the alkaline alpha-galactosidase ClAGA2 was identified as the key factor controlling stachyose and Raf hydrolysis, and it was determined to be specifically expressed in the vascular bundle. Analysis of transgenic plants confirmed that ClAGA2 controls fruit Raf hydrolysis and reduces sugar content in fruits. Two single-nucleotide polymorphisms (SNPs) within the ClAGA2 promoter affect the recruitment of the transcription factor ClNF-YC2 (nuclear transcription factor Y subunit C) to regulate ClAGA2 expression. Moreover, this study demonstrates that C. lanatus Sugars Will Eventually Be Exported Transporter 3 (ClSWEET3) and Tonoplast Sugar Transporter (ClTST2) participate in plasma membrane sugar transport and sugar storage in fruit cell vacuoles, respectively. Knocking out ClAGA2, ClSWEET3, and ClTST2 affected fruit sugar accumulation. Genomic signatures indicate that the selection of ClAGA2, ClSWEET3, and ClTST2 for carbohydrate partitioning led to the derivation of modern sweet watermelon from non-sweet ancestors during domestication.

Response of photosynthate distribution in potato plants to different LED spectra

Although light is essential to photosynthesis, few studies have examined the effects of different LED spectra on photosynthate distribution in potato plants. Therefore, we exposed tuberising potato plants to white (W), red (R), blue (B) and green (G) LED treatments and compared tuber development and carbohydrate partitioning among the plants. R-treated plants had greater photosynthetic leaf area during tuber development compared with those under other treatments, thus enhancing assimilation. Although R-treated plants had higher 13C assimilation in the leaves, stems and roots than those under B treatment, there was no difference in partitioning of 13C assimilation and yield in the tubers of each plant between R and B treatments. For the tuber size, R-treated plants had a higher ratio of large tubers (>20 g) and a lower ratio of small (2–20 g) and medium-sized (10–20 g) tubers than those under W. B-treated plants had more medium-sized and large tubers than those under W. The reason may be that plants under R treatment distributed more assimilated 13C in their first tuber than those under other treatments. By contrast, plants under B balanced photosynthate distribution among their tubers. Leaves under G treatment had lower photosynthetic efficiency and ΦPSII than those under W, R or B treatment, which resulted in lower 13C photosynthate allocation in organs and lower tuber yield per plant than in R and B treatments. Overall, R treatment promoted 13C assimilation and led to more large tubers than other treatments. B-treated plants distributed more photosynthates into tubers rather than other organs and showed balanced tuber development.

Overexpression of HvCslF6 in barley grain alters carbohydrate partitioning plus transfer tissue and endosperm development

Overexpression of the HvCslF6 gene in hull-less barley grain perturbs sucrose uptake and allocation, and impairs transfer tissue and endosperm development