Reproductive trade-offs of the estuarine copepod Eurytemora affinis under different thermal and haline regimes

Copepod females invest a quantity of resources in their reproduction. Depending on several biotic and abiotic factors and their evolutionary history a trade-off can be commonly observed between producing a large number of smaller offspring or a small number of larger offspring. In this study, a multi-generational approach was applied to determine whether a trade-off between clutch size and egg size existed in the copepod Eurytemora affinis under different controlled conditions of temperature and salinity. This protocol was based on the follow-up of reproductive (Clutch Size ‘CS’, Egg Diameter ‘ED’) and morphological (Prosome Length ‘PL’) traits during several generations. Copepods were acclimated to cold (7 °C) and warm (20 °C) temperatures, and then their reproductive output was tested at the higher temperature of 24 °C. CS and ED were positively correlated to PL, so as a first step linear regressions between each reproductive trait and female PL were performed. The residuals from the regression lines of CS and ED with PL were calculated to remove the effect of female size. When the normalized data (residuals) of CS and ED plotted together a negative relationship between egg size and egg number revealed the existence of a trade-off. Copepod populations initially acclimated to cold temperature are commonly characterized by relatively smaller CS and larger ED. Conversely, warm temperature adapted females produced relatively larger CS and smaller ED. After transfer to a temperature of 24 °C, the ED did not change but the CS showed high variability indicating stressful conditions and no trade-off was observed. These observations suggest that E. affinis is able to modulate its reproduction depending on the encountered temperature. It seems that this copepod species can shift between a K- and an r-strategy in response to colder or warmer conditions. In a late winter-early spring like cold temperature, copepod females seem to invest more on offspring quality by producing relatively larger eggs. This ecological strategy ensures a high recruitment of the spring generation that is responsible for the strength of the maximum population size usually observed in late spring-early summer (May–June). To the contrary, at summer-like temperature, where the population density decreases significantly in the Seine estuary, copepod females seem to switch from K to r strategy by favoring offspring number compared to offspring size. Finally, the use of a higher temperature of 24 °C seems to disrupt the observed reproductive trade-off even after several generations. These results suggest that a switching between K- or r-strategy of E. affinis depends highly on temperature effects. The effect of salinity increase during a summer-like temperature of 20 °C as well as after transfer to 24 °C decreased PL and CS but the ED did not change significantly.

Susceptibility of the Non-Targeted Crustacean Eurytemora affinis to the Endocrine Disruptor Tebufenozide: A Transcriptomic Approach

Copepods are zooplanktonic crustaceans ubiquitously widespread in aquatic systems. Although they are not the target, copepods are exposed to a wide variety of pollutants such as insect growth regulators (IGRs). The aim of this study was to investigate the molecular response of a non-targeted organism, the copepod Eurytemora affinis, to an IGR. Adult males and females were exposed to two sub-lethal concentrations of tebufenozide (TEB). Our results indicate a sex-specific response with a higher sensitivity in males, potentially due to a differential activation of stress response pathways. In both sexes, exposure to TEB triggered similar pathways to those found in targeted species by modulating the transcription of early and late ecdysone responsive genes. Among them were genes involved in cuticle metabolism, muscle contraction, neurotransmission, and gametogenesis, whose mis-regulation could lead to moult, locomotor, and reproductive impairments. Furthermore, genes involved in epigenetic processes were found in both sexes, which highlights the potential impact of exposure to TEB on future generations. This work allows identification of (i) potential biomarkers of ecdysone agonists and (ii) further assessment of putative physiological responses to characterize the effects of TEB at higher biological levels. The present study reinforces the suitability of using E. affinis as an ecotoxicological model.

Promotion of the Development of Sentinel Species in the Water Column: Example Using Body Size and Fecundity of the Egg-Bearing Calanoid Copepod Eurytemora affinis

The development of sentinel species in aquatic ecosystems is mostly based on benthic organisms; however, organisms living in water column such as zooplankton have received less attention, except for some cladocerans. In this paper, a new ecological indicator based on simple measurements of the size and fecundity of egg-bearing calanoid copepods is developed. The well-studied estuarine copepod Eurytemora affinis is used to illustrate this new framework. A large database obtained from laboratory experiments developed under different conditions is used to define a reference regression line between clutch size (CS) and prosome length (PL). The same database allowed one to confirm that the coefficient of variation (CV) of CS is an adequate estimator of the accumulated stress at population level. The CV of PL shows very little variability in all experimental and field conditions. The values of CS and PL obtained from the Seine, Loire, Gironde, Scheldt and Elbe estuaries in Europe are compared to the reference regression line. A quality index (QI) is calculated as a percentage of difference between the observed and the predicted CS. The QI classified 19 samples collected in the Seine estuary between 2004 and 2010 into four classes according to the physiological condition of the copepod female. A single sampling from June 2004 (5.26%) showed a very good condition, whereas 57.9% of the sampling dates confirmed good conditions. On the other hand, four sampling dates were associated to very bad conditions and three sampling dates indicated bad conditions. Seven additional samples obtained from other European estuaries between 2006 and 2009 were also used. Females showing poor conditions were observed in the early spring of 2005 and 2008 as well as during the month of November. These years were characterized by very strong climatic anomalies with a very cold late winter in 2005 and a warm winter in 2008. Therefore, it seems that the QI perfectly reflected the strong stress caused by the sudden change in hydro-climatic conditions that have certainly affected the physiology of copepod females and probably the availability of food. The new indicator is very simple to calculate and can be generalised to several aquatic ecosystems (fresh water and brackish water) by targeting the dominating egg-bearing calanoid copepods. As in the case of E. affinis, the development of sentinel species based on copepods or cladocerans can enrich ecological and ecotoxicological studies given their capacity to integrate the variability of their habitats’ quality at the individual and population levels.