nutrient exchange
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Lab on a Chip ◽  
2022 ◽  
Author(s):  
Sophie R Cook ◽  
Hannah B Musgrove ◽  
Amy L Throckmorton ◽  
Rebecca R. Pompano

Fluid flow is an integral part of microfluidic and organ-on-chip technology, ideally providing biomimetic fluid, cell, and nutrient exchange as well as physiological or pathological shear stress. Currently, many of...


PLoS ONE ◽  
2021 ◽  
Vol 16 (9) ◽  
pp. e0257017
Author(s):  
Alexa K. Hoke ◽  
Guadalupe Reynoso ◽  
Morgan R. Smith ◽  
Malia I. Gardner ◽  
Dominique J. Lockwood ◽  
...  

Microbial interactions in harmful algal bloom (HAB) communities have been examined in marine systems, but are poorly studied in fresh waters. To investigate HAB-microbe interactions, we isolated bacteria with close associations to bloom-forming cyanobacteria, Microcystis spp., during a 2017 bloom in the western basin of Lake Erie. The genomes of five isolates (Exiguobacterium sp. JMULE1, Enterobacter sp. JMULE2, Deinococcus sp. JMULE3, Paenibacillus sp. JMULE4, and Acidovorax sp. JMULE5.) were sequenced on a PacBio Sequel system. These genomes ranged in size from 3.1 Mbp (Exiguobacterium sp. JMULE1) to 5.7 Mbp (Enterobacter sp. JMULE2). The genomes were analyzed for genes relating to critical metabolic functions, including nitrogen reduction and carbon utilization. All five of the sequenced genomes contained genes that could be used in potential signaling and nutrient exchange between the bacteria and cyanobacteria such as Microcystis. Gene expression signatures of algal-derived carbon utilization for two isolates were identified in Microcystis blooms in Lake Erie and Lake Tai (Taihu) at low levels, suggesting these organisms are active and may have a functional role during Microcystis blooms in aggregates, but were largely missing from whole water samples. These findings build on the growing evidence that the bacterial microbiome associated with bloom-forming algae have the functional potential to contribute to nutrient exchange within bloom communities and interact with important bloom formers like Microcystis.


Pathogens ◽  
2021 ◽  
Vol 10 (2) ◽  
pp. 146
Author(s):  
Hafij Al Mahmud ◽  
Jiwasmika Baishya ◽  
Catherine A. Wakeman

Cystic fibrosis (CF) is a genetic disease frequently associated with chronic lung infections caused by a consortium of pathogens. It is common for auxotrophy (the inability to biosynthesize certain essential metabolites) to develop in clinical isolates of the dominant CF pathogen Pseudomonas aeruginosa, indicating that the CF lung environment is replete in various nutrients. Many of these nutrients are likely to come from the host tissues, but some may come from the surrounding polymicrobial community within the lungs of CF patients as well. To assess the feasibility of nutrient exchange within the polymicrobial community of the CF lung, we selected P. aeruginosa and Staphylococcus aureus, two of the most prevalent species found in the CF lung environment. By comparing the polymicrobial culture of wild-type strains relative to their purine auxotrophic counterparts, we were able to observe metabolic complementation occurring in both P. aeruginosa and S. aureus when grown with a purine-producing cross-species pair. While our data indicate that some of this complementation is likely derived from extracellular DNA freed by lysis of S. aureus by the highly competitive P. aeruginosa, the partial complementation of S. aureus purine deficiency by P. aeruginosa demonstrates that bidirectional nutrient exchange between these classic competitors is possible.


Chemosphere ◽  
2020 ◽  
pp. 128665
Author(s):  
Fenfang Wang ◽  
Peng Cheng ◽  
Nengwang Chen ◽  
Yi-Ming Kuo

2020 ◽  
Vol 15 (1) ◽  
pp. 282-292
Author(s):  
Corey Nelson ◽  
Ana Giraldo-Silva ◽  
Ferran Garcia-Pichel

2020 ◽  
Vol 11 ◽  
Author(s):  
Xiaoming Chen ◽  
Zixiang Yang ◽  
Hang Chen ◽  
Qian Qi ◽  
Juan Liu ◽  
...  
Keyword(s):  

2020 ◽  
Author(s):  
Lena M. Müller ◽  
Lidia Campos-Soriano ◽  
Veronique Levesque-Tremblay ◽  
Armando Bravo ◽  
Dierdra A. Daniels ◽  
...  

AbstractArbuscular mycorrhizal (AM) symbiosis is a mutually beneficial association of plants and fungi of the sub-phylum Glomeromycotina. The endosymbiotic AM fungi colonize the inner cortical cells of the roots, where they form branched hyphae called arbuscules that function in nutrient exchange with the plant. To support arbuscule development and subsequently bidirectional nutrient exchange, the root cortical cells undergo substantial transcriptional re-programming. REDUCED ARBUSCULAR MYCORRHIZA 1 (RAM1), studied in several dicot plant species, is a major regulator of this cortical cell transcriptional program. Here, we generated ram1 mutants and RAM1 overexpressors in a monocot, Brachypodium distachyon. The AM phenotypes of two ram1 lines revealed that RAM1 is only partly required to enable arbuscule development in B. distachyon. Transgenic lines constitutively overexpressing BdRAM1 showed constitutive expression of AM-inducible genes even in the shoots. Following inoculation with AM fungi, BdRAM1-overexpressing roots showed higher arbuscule densities relative to controls, indicating the potential to manipulate the relative proportion of symbiotic interfaces via modulation of RAM1. However, the overexpressors also show altered expression of hormone biosynthesis genes and aberrant growth patterns including stunted bushy shoots and poor seed set. While these phenotypes possibly provide additional clues about BdRAM1’s scope of influence, they also indicate that directed approaches to increase the density of symbiotic interfaces will require a more focused, potentially cell-type specific manipulation of transcription factor gene expression.


2020 ◽  
Vol 30 (10) ◽  
pp. R437-R439
Author(s):  
Vincent S.F.T. Merckx ◽  
Sofia I.F. Gomes
Keyword(s):  

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