scholarly journals Genomic signatures of Lake Erie bacteria suggest interaction in the Microcystis phycosphere

PLoS ONE ◽  
2021 ◽  
Vol 16 (9) ◽  
pp. e0257017
Author(s):  
Alexa K. Hoke ◽  
Guadalupe Reynoso ◽  
Morgan R. Smith ◽  
Malia I. Gardner ◽  
Dominique J. Lockwood ◽  
...  
Keyword(s):  
Lake Erie ◽  
Harmful Algal Bloom ◽  
Microbial Interactions ◽  
Carbon Utilization ◽  
Western Basin ◽  
Nutrient Exchange ◽  
Genomic Signatures ◽  
Metabolic Functions ◽  
Bacterial Microbiome ◽  
Microbe Interactions

Microbial interactions in harmful algal bloom (HAB) communities have been examined in marine systems, but are poorly studied in fresh waters. To investigate HAB-microbe interactions, we isolated bacteria with close associations to bloom-forming cyanobacteria, Microcystis spp., during a 2017 bloom in the western basin of Lake Erie. The genomes of five isolates (Exiguobacterium sp. JMULE1, Enterobacter sp. JMULE2, Deinococcus sp. JMULE3, Paenibacillus sp. JMULE4, and Acidovorax sp. JMULE5.) were sequenced on a PacBio Sequel system. These genomes ranged in size from 3.1 Mbp (Exiguobacterium sp. JMULE1) to 5.7 Mbp (Enterobacter sp. JMULE2). The genomes were analyzed for genes relating to critical metabolic functions, including nitrogen reduction and carbon utilization. All five of the sequenced genomes contained genes that could be used in potential signaling and nutrient exchange between the bacteria and cyanobacteria such as Microcystis. Gene expression signatures of algal-derived carbon utilization for two isolates were identified in Microcystis blooms in Lake Erie and Lake Tai (Taihu) at low levels, suggesting these organisms are active and may have a functional role during Microcystis blooms in aggregates, but were largely missing from whole water samples. These findings build on the growing evidence that the bacterial microbiome associated with bloom-forming algae have the functional potential to contribute to nutrient exchange within bloom communities and interact with important bloom formers like Microcystis.

scholarly journals Roles of Nutrient Limitation on Western Lake Erie CyanoHAB Toxin Production

Toxins ◽  
2021 ◽  
Vol 13 (1) ◽  
pp. 47
Author(s):  
Malcolm A. Barnard ◽  
Justin D. Chaffin ◽  
Haley E. Plaas ◽  
Gregory L. Boyer ◽  
Bofan Wei ◽  
...  
Keyword(s):  
Nutrient Limitation ◽  
Biomass Production ◽  
Lake Erie ◽  
Harmful Algal Bloom ◽  
Toxin Production ◽  
Western Basin ◽  
N Limitation ◽  
P Limitation ◽  
Western Lake ◽  
Western Lake Erie

Cyanobacterial harmful algal bloom (CyanoHAB) proliferation is a global problem impacting ecosystem and human health. Western Lake Erie (WLE) typically endures two highly toxic CyanoHABs during summer: a Microcystis spp. bloom in Maumee Bay that extends throughout the western basin, and a Planktothrix spp. bloom in Sandusky Bay. Recently, the USA and Canada agreed to a 40% phosphorus (P) load reduction to lessen the severity of the WLE blooms. To investigate phosphorus and nitrogen (N) limitation of biomass and toxin production in WLE CyanoHABs, we conducted in situ nutrient addition and 40% dilution microcosm bioassays in June and August 2019. During the June Sandusky Bay bloom, biomass production as well as hepatotoxic microcystin and neurotoxic anatoxin production were N and P co-limited with microcystin production becoming nutrient deplete under 40% dilution. During August, the Maumee Bay bloom produced microcystin under nutrient repletion with slight induced P limitation under 40% dilution, and the Sandusky Bay bloom produced anatoxin under N limitation in both dilution treatments. The results demonstrate the importance of nutrient limitation effects on microcystin and anatoxin production. To properly combat cyanotoxin and cyanobacterial biomass production in WLE, both N and P reduction efforts should be implemented in its watershed.

scholarly journals Methane and nitrous oxide measured throughout Lake Erie over all seasons indicate highest emissions from the eutrophic Western Basin

2020 ◽  
Vol 46 (6) ◽  
pp. 1604-1614
Author(s):  
Julianne M. Fernandez ◽  
Amy Townsend-Small ◽  
Arthur Zastepa ◽  
Susan B. Watson ◽  
Jay A. Brandes
Keyword(s):  
Nitrous Oxide ◽  
Lake Erie ◽  
Western Basin

scholarly journals Elevated Incidences of Antimicrobial Resistance and Multidrug Resistance in the Maumee River (Ohio, USA), a Major Tributary of Lake Erie

2021 ◽  
Vol 9 (5) ◽  
pp. 911
Author(s):  
Maitreyee Mukherjee ◽  
Leah Marie ◽  
Cheyenne Liles ◽  
Nadia Mustafa ◽  
George Bullerjahn ◽  
...  
Keyword(s):  
Multidrug Resistance ◽  
Antimicrobial Resistance ◽  
High Resistance ◽  
Lake Erie ◽  
Control Measures ◽  
Industrial Water ◽  
Western Basin ◽  
E Coli ◽  
Major Tributary ◽  
Very High

Maumee River, the major tributary in the western basin of Lake Erie, serves as one of major sources of freshwater in the area, supplying potable, recreational, and industrial water. In this study we collected water samples from four sites in the Maumee River Bay between 2016–2017 and E. coli was isolated, enumerated, and analyzed for antimicrobial resistance (AMR) and multidrug resistance (MDR). Strikingly, 95% of the total isolates were found to be resistant to at least one antibiotic. A very high resistance to the drugs cephalothin (95.3%), ampicillin (38.3%), tetracycline (8.8%), gentamicin (8.2%), ciprofloxacin (4.2%), cefoperazone (4%), and sulfamethoxazole (1.5%) was observed within isolates from all four sampling sites. Percentages of AMR and MDR was consistently very high in the summer and fall months, whereas it was observed to be lowest in the winter. A remarkably high number of the isolates were detected to be MDR—95% resistant to ≥1 antibiotic, 43% resistant to ≥2 antibiotics, 15% resistant to ≥3 antibiotics, 4.9% resistant to ≥4 antibiotic and 1.2% resistant to ≥5 antibiotics. This data will serve in better understanding the environmental occurrence and dissemination of AMR/MDR in the area and assist in improving and establishing control measures.

scholarly journals Spatial metabolomics of in situ, host-microbe interactions

2019 ◽  
Author(s):  
Benedikt K Geier ◽  
Emilia Sogin ◽  
Dolma Michellod ◽  
Moritz Janda ◽  
Mario Kompauer ◽  
...  
Keyword(s):  
Microbial Interactions ◽  
Host Cells ◽  
Metabolic Phenotype ◽  
Community Members ◽  
Metabolic Phenotypes ◽  
Culture Independent ◽  
Microbe Interactions ◽  
Host Microbe Interactions ◽  
Atmospheric Pressure Mass Spectrometry

Spatial metabolomics describes the location and chemistry of small molecules involved in metabolic phenotypes, defense molecules and chemical interactions in natural communities. Most current techniques are unable to spatially link the genotype and metabolic phenotype of microorganisms in situ at a scale relevant to microbial interactions. Here, we present a spatial metabolomics pipeline (metaFISH) that combines fluorescence in situ hybridization (FISH) microscopy and high-resolution atmospheric pressure mass spectrometry imaging (AP-MALDI-MSI) to image host-microbe symbioses and their metabolic interactions. metaFISH aligns and integrates metabolite and fluorescent images at the micrometer-scale for a spatial assignment of host and symbiont metabolites on the same tissue section. To illustrate the advantages of metaFISH, we mapped the spatial metabolome of a deep-sea mussel and its intracellular symbiotic bacteria at the scale of individual epithelial host cells. Our analytical pipeline revealed metabolic adaptations of the epithelial cells to the intracellular symbionts, a variation in metabolic phenotypes in one symbiont type, and novel symbiosis metabolites. metaFISH provides a culture-independent approach to link metabolic phenotypes to community members in situ - a powerful tool for microbiologists across fields.

scholarly journals Both Leaf Properties and Microbe-Microbe Interactions Influence Within-Species Variation in Bacterial Population Diversity and Structure in the Lettuce (Lactuca Species) Phyllosphere

2010 ◽  
Vol 76 (24) ◽  
pp. 8117-8125 ◽  
Author(s):  
Paul J. Hunter ◽  
Paul Hand ◽  
David Pink ◽  
John M. Whipps ◽  
Gary D. Bending
Keyword(s):  
Community Structure ◽  
Bacterial Community ◽  
Bacterial Community Structure ◽  
Population Diversity ◽  
Microbial Interactions ◽  
Soluble Carbohydrates ◽  
Rrna Gene ◽  
Species Variation ◽  
Clone Libraries ◽  
Microbe Interactions

ABSTRACT Morphological and chemical differences between plant genera influence phyllosphere microbial populations, but the factors driving within-species variation in phyllosphere populations are poorly understood. Twenty-six lettuce accessions were used to investigate factors controlling within-species variation in phyllosphere bacterial populations. Morphological and physiochemical characteristics of the plants were compared, and bacterial community structure and diversity were investigated using terminal restriction fragment length polymorphism (T-RFLP) profiling and 16S rRNA gene clone libraries. Plant morphology and levels of soluble carbohydrates, calcium, and phenolic compounds (which have long been associated with plant responses to biotic stress) were found to significantly influence bacterial community structure. Clone libraries from three representative accessions were found to be significantly different in terms of both sequence differences and the bacterial genera represented. All three libraries were dominated by Pseudomonas species and the Enterobacteriaceae family. Significant differences in the relative proportions of genera in the Enterobacteriaceae were detected between lettuce accessions. Two such genera (Erwinia and Enterobacter) showed significant variation between the accessions and revealed microbe-microbe interactions. We conclude that both leaf surface properties and microbial interactions are important in determining the structure and diversity of the phyllosphere bacterial community.

scholarly journals Does behavioural thermoregulation underlie seasonal movements in Lake Erie walleye?

2018 ◽  
Vol 75 (3) ◽  
pp. 488-496 ◽  
Author(s):  
Graham D. Raby ◽  
Christopher S. Vandergoot ◽  
Todd A. Hayden ◽  
Matthew D. Faust ◽  
Richard T. Kraus ◽  
...  
Keyword(s):  
Lake Erie ◽  
Seasonal Migration ◽  
Central Basin ◽  
Behavioural Thermoregulation ◽  
Long Distance ◽  
Western Basin ◽  
Eastern Basin ◽  
Temperature Records ◽  
Thermal Preferences

Thermoregulation is presumed to be a widespread determinant of behaviour in fishes, but has not often been investigated as a mechanism shaping long-distance migrations. We used acoustic telemetry and animal-borne thermal loggers to test the hypothesis that seasonal migration in adult walleye (Sander vitreus) in Lake Erie is size- and (or) sex-specific and related to behavioural thermoregulation. Female walleye migrated out of the warm, shallow western basin earlier than did males and were 1.8 times more likely to be detected on acoustic receivers in the deeper and cooler eastern basin. The few fish that remained in the western basin were restricted to a smaller range of higher temperatures (≥20 °C) than those that migrated to the central and eastern basins (∼16–21 °C). However, temperature records from walleye in the central basin were nearly indistinguishable from those in the eastern basin, suggesting thermal preferences alone could not explain migration to the eastern basin. As such, our effort to understand the mechanisms that cause migratory behaviours has generated mixed evidence on the role of temperature and that factors like foraging opportunities may have synergistic roles in the migration.

scholarly journals Detoured flight direction responses along the southwest coast of Lake Erie by night-migrating birds

The Auk ◽  
2019 ◽  
Vol 136 (3) ◽  
Author(s):  
David V Gesicki ◽  
Erica L Cech ◽  
Verner P Bingman
Keyword(s):  
Lake Erie ◽  
Migratory Birds ◽  
Western Basin ◽  
Good Opportunity ◽  
Infrared Observations ◽  
The North ◽  
Flight Direction ◽  
Breeding Grounds ◽  
Topographical Feature ◽  
Migrating Birds

Abstract Migratory birds encounter the Great Lakes while moving through eastern North America toward breeding grounds to the North, which offers a good opportunity to study variation in migratory behavior as birds face a potentially influencing topographical feature. Using passive infrared technology, we documented the direction of relatively low-flying, nocturnal, mostly passerine migration in spring along the southern coast of Lake Erie’s western basin. We examined the extent to which spring migrants flew across Lake Erie as a continuation of the inland, northeasterly broad front migratory direction, as determined by weather radar and infrared observations, or displayed a tendency to deviate to more closely follow the direction of the coastline. We found that an estimated 62% of all low-flying migrants deviated their flight directions toward the coast of Lake Erie at 2 coastal sites, Cedar Point and Ottawa, which were characterized by northwest–southeast oriented coastlines. Migrants at a third coastal location, Maumee Bay, which has a more east–west oriented coastline, did not display similarly deviated flight directions. We found that even when winds were energetically favorable for a lake crossing, many migrants still routinely displayed deviated flight directions that approached paralleling the coastline. Further, the mean flight direction at one site, Ottawa, shifted more in the direction of the coastline as the night progressed, suggesting that time of night could influence the shift to a more coastal flight direction. The data indicate that the western basin of Lake Erie acts as a salient topographical feature influencing the flight directions of nocturnal migrants. The data further suggest that birds are making active decisions while in flight, based on current environmental and physiological conditions, about whether to continue to cross Lake Erie or take a coastal detour.

scholarly journals Exploring possible associations of the intestine bacterial microbiome with the pre-weaned weight gaining performance of piglets in intensive pig production

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Xinghua Ding ◽  
Wensheng Lan ◽  
Gang Liu ◽  
Hengjia Ni ◽  
Ji-Dong Gu
Keyword(s):  
Weight Gain ◽  
Bacterial Community ◽  
16S Rrna ◽  
Illumina Miseq ◽  
Microbial Interactions ◽  
Intestinal Microbiome ◽  
Weaned Piglets ◽  
Pig Production ◽  
Host Health ◽  
Bacterial Microbiome

Abstract The pre-weaned weight gain is an important performance trait of pigs in intensive pig production. The bacterial microbiome inside the host is vital to host health and growth performance. The purpose of this study was to explore the possible associations of the intestinal microbiome with the pre-weaned weight gain in intensive pig production. In this study, several anatomical sites (jejunum, ileum, cecum, and colon) were examined for bacterial microbiome structure using 16S rRNA V4-V5 region sequencing with Illumina Miseq. The results showed that the microbial richness (estimated by Chao1 index) in jejunum was positively correlated with the pre-weaned weight gain. This study also revealed that the Firmicutes and Bacteroidetes in colon were the weight gaining-related phyla; while the Selenomonas and Moraxella in ileum and the Lactobacillus in both cecum and colon were the weight gaining-related genera for the pre-weaned piglets in intensive pig prodution. Several intra-microbial interactions within commensal microbiome correlated with the pre-weaned weight gain were excavated, as well. Overall, this study provides an expanded view of the commensal bacterial community inside four anatomical intestinal sites of the commercial piglets and the associations of the intestinal microbiome with the pre-weaned weight gaining performance in intensive pig production.

scholarly journals Microbial interactions in the mosquito gut determine Serratia colonization and blood-feeding propensity

2020 ◽  
Vol 15 (1) ◽  
pp. 93-108
Author(s):  
Elena V. Kozlova ◽  
Shivanand Hegde ◽  
Christopher M. Roundy ◽  
George Golovko ◽  
Miguel A. Saldaña ◽  
...  
Keyword(s):  
Vector Competence ◽  
Inverse Correlation ◽  
Blood Feeding ◽  
Microbial Interactions ◽  
Host Behavior ◽  
Microbiome Analysis ◽  
Microbe Interactions ◽  
Host Microbe Interactions ◽  
Native Host ◽  
Mosquito Behavior

AbstractHow microbe–microbe interactions dictate microbial complexity in the mosquito gut is unclear. Previously we found that, Serratia, a gut symbiont that alters vector competence and is being considered for vector control, poorly colonized Aedes aegypti yet was abundant in Culex quinquefasciatus reared under identical conditions. To investigate the incompatibility between Serratia and Ae. aegypti, we characterized two distinct strains of Serratia marcescens from Cx. quinquefasciatus and examined their ability to infect Ae. aegypti. Both Serratia strains poorly infected Ae. aegypti, but when microbiome homeostasis was disrupted, the prevalence and titers of Serratia were similar to the infection in its native host. Examination of multiple genetically diverse Ae. aegypti lines found microbial interference to S. marcescens was commonplace, however, one line of Ae. aegypti was susceptible to infection. Microbiome analysis of resistant and susceptible lines indicated an inverse correlation between Enterobacteriaceae bacteria and Serratia, and experimental co-infections in a gnotobiotic system recapitulated the interference phenotype. Furthermore, we observed an effect on host behavior; Serratia exposure to Ae. aegypti disrupted their feeding behavior, and this phenotype was also reliant on interactions with their native microbiota. Our work highlights the complexity of host–microbe interactions and provides evidence that microbial interactions influence mosquito behavior.

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