The objective of this study was to identify the knowledge outcome of students majoring in physical and health education at Al-Balqa Applied University studying the principles of sports training, nutrition for athletes, and sports anatomy during the COVID-19 pandemic. The study adopted the descriptive analytical approach, where the curricula for the principles of sports training, the nutrition curriculum for athletes, and the sports anatomy curriculum were chosen, which were taught during the second semester of the academic year 2020/2021. In order to determine the degree of the knowledge outcome of the students, the study adopted the purposive sampling method in selecting the study sample. The students of physical and health education specialization at Al-Balqa Applied University were identified, where the sample consisted of 135 male and female students. The study concluded that the ratio of the total knowledge outcome to the knowledge outcome was at a moderate level with a percentage of 61.1, while it was low in the sports Anatomy curriculum 58.8. The results showed that there were statistically significant differences in favor of the students of Princess Alia College, and the results showed that there were no statistically significant differences between males and females in the nutrition and sports anatomy domain, while there were statistically significant differences in favor of males in the principles of sports training domain. This study increased the depth of understanding of knowledge outcome during the COVID-19 pandemic, whereas other studies were performed in normal non-stressful situations.