scholarly journals Effect of Chicken Bone Extract Powder on Epididymal Sperm Quality of Male Wistar Rats

2021 ◽  
Vol 26 (2) ◽  
pp. 74
Author(s):  
Kakanang Buranaamnuay ◽  
A Kettawan ◽  
C Changsangfa ◽  
S Aiemongkot
Keyword(s):  
Sperm Quality ◽  
Reproductive Function ◽  
Calcium Deficiency ◽  
Standard Diet ◽  
Hypotonic Stress ◽  
Male Reproductive Function ◽  
Chicken Bone ◽  
Male Wistar Rats

Calcium is one of the minerals that are essential for male reproductive function. Calcium deficiency adversely affects spermatogenesis, normal sperm function and results in infertility. The sperm quality of rats fed a standard diet containing chicken bone extract powder (BEP) was assessed in the present study. Twenty male 8-week-old rats, Wistar strain, were randomized by weight into two groups of ten rats each and fed ad libitum a standard diet containing calcium carbonate (CaCO<sub>3</sub>, control) or chicken BEP;  both were equivalent to 0.5% calcium. At the end of the 7-week consumption, the net body weight gains measured in control (101.33±21.81 g) and chicken BEP groups (100.74±26.80 g) were not significantly different (P&gt;0.05). The in vitro sperm quality in terms of concentration, motility, viability, resistance to hypotonic stress, acrosomal reaction ability and morphology was comparable between control and chicken BEP (all were P&gt;0.05). The results suggest that chicken BEP addition into feeds is an alternative calcium source that is as effective but less expensive as CaCO<sub>3,</sub> a commercial calcium (fortificant). At least, it has no detrimental effect on male reproductive function.

The Prevalence of Bacteriospermia in Infertile Men and Association with Semen Quality in Southwestern Iran

2020 ◽  
Vol 20 (2) ◽  
pp. 198-202 ◽  
Author(s):  
Mohammad Motamedifar ◽  
Yalda Malekzadegan ◽  
Parisa Namdari ◽  
Behzad Dehghani ◽  
Bahia Namavar Jahromi ◽  
...  
Keyword(s):  
Semen Quality ◽  
Sperm Quality ◽  
Reproductive Function ◽  
World Health ◽  
Public Health Issue ◽  
Control Group ◽  
Microbiological Analysis ◽  
Male Reproductive Function ◽  
Infertile Men ◽  
Southwestern Iran

Introduction: Infertility considered as a social and public health issue and estimated that most of these infertile couples are residents of developing countries. Infectious diseases including the history of Sexually Transmitted Infections (STIs) may impact on male reproductive function. Therefore, the present study aimed to investigate the prevalence of bacterial contaminants of semen and probable association with sperm quality of infertile men in Iranian population. Methods: The study population consisted of 200 infertile men and 150 fertile men attending an infertility Center in southwestern Iran during the study period in 2015. The assessment of sperm parameters was according to the World Health Organization (WHO) guidelines. The presumptive pathogens were identified using standard microbiology tests and confirmed by specific PCR primers. Results: The prevalence of bacteriospermia in the semen of the infertile group was significantly higher than that in the fertile group (48% vs. 26.7%, P <0.001). The microbiological analysis of samples showed that the most abundant species of bacteria in semen of infertile men were Chlamydia trachomatis (12.5%) followed by Neisseria gonorrhoeae (11%). On the other hand, in the control group, Lactobacillus spp. (17.3%) was the most isolated pathogen. Results showed that the presence of N. gonorrhoeae, C. trachomatis, Mycoplasma genitalium, Haemophilus, and Klebsiella was significantly associated with sperm abnormality. Conclusion: Based on our findings, it seems that bacteriospermia is associated with alterations in the properties of semen which may lead to a decrease in the fertilization potential of sperm. Therefore, immediate and appropriate treatment is necessary before investigating every other possible cause of infertility.

Aluminum at environmental concentrations affects the sperm quality of the freshwater teleost Astyanax altiparanae: An in vitro study

2021 ◽  
Vol 243 ◽  
pp. 108983
Author(s):  
Cecília Bertacini de Assis ◽  
Giovana Souza Branco ◽  
Renata Guimarães Moreira ◽  
João Paulo Silva Pinheiro
Keyword(s):  
Sperm Quality ◽  
In Vitro Study ◽  
Vitro Study ◽  
Freshwater Teleost ◽  
Environmental Concentrations

scholarly journals Effect of Different Exercise Loads on Testicular Oxidative Stress and Reproductive Function in Obese Male Mice

2020 ◽  
Vol 2020 ◽  
pp. 1-13 ◽  
Author(s):  
Xuejie Yi ◽  
Donghui Tang ◽  
Shicheng Cao ◽  
Tao Li ◽  
Haining Gao ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Protein Expression ◽  
Sperm Quality ◽  
Reproductive Function ◽  
High Load ◽  
Serum Testosterone Level ◽  
Male Mice ◽  
Male Reproductive Function ◽  
Mrna And Protein Expression ◽  
Moderate Load

This study is aimed at investigating the effect of different exercise loads on the reproductive function of obese male mice and the underlying mechanisms. Male mice with high-fat diet-induced obesity were divided into obesity control (OC), obesity moderate-load exercise (OME), and obesity high-load exercise (OHE) groups. The OME and OHE groups were subjected to swimming exercise 5 days per week over a duration of 8 weeks, with the exercise load progressively increased to 2 h per day in the OME group and 2 h twice per day in the OHE group. In the OC group mice without exercise regimen, we observed a decrease in mRNA expression of antioxidant enzymes, increase in free radical products, upregulation of mRNA and protein expression of nuclear factor-κB and proinflammatory cytokines, inhibition of mRNA and protein expression of testosterone synthases, decrease in the serum testosterone level and sperm quality, and increase in sperm apoptosis. Although both moderate-load exercise and high-load exercise reduced body fat, only moderate-load exercise effectively alleviated obesity-induced oxidative stress, downregulated the expression of nuclear factor-κB and proinflammatory cytokines, and reversed the decrease in mRNA and protein expression of testosterone synthases, serum testosterone level, and sperm quality. These changes were not observed in the OHE group mice. Obesity-induced testicular oxidative stress and inflammatory response decreased testosterone synthesis and sperm quality. Moderate-load exercise alleviated the negative effect of obesity on male reproductive function by decreasing testicular oxidative stress and inflammatory responses. Although high-load exercise effectively reduced body fat, its effects on alleviating oxidative stress and improving male reproductive function were limited.

Development of sperm sexing and associated assisted reproductive technology for sex preselection of captive bottlenose dolphins (Tursiops truncatus)

2006 ◽  
Vol 18 (3) ◽  
pp. 319 ◽  
Author(s):  
J. K. O'Brien ◽  
T. R. Robeck
Keyword(s):  
Tursiops Truncatus ◽  
Sperm Quality ◽  
Bottlenose Dolphins ◽  
Sperm Concentration ◽  
Motility Index ◽  
Liquid Storage ◽  
Directional Freezing

Research was conducted to develop sperm sorting and novel sperm preservation methodologies for sex predetermination in the bottlenose dolphin (Tursiops truncatus) using artificial insemination. In Study 1, the effect of seminal plasma (SP), sperm concentration and freezing rate (FR) on in vitro sperm quality of liquid-stored, non-sorted spermatozoa was examined. There was no effect (P > 0.05) of prefreeze SP addition on post-thaw quality (progressive motility, kinetic rating, sperm motility index (SMI), viability and acrosome integrity). Post-thaw motility parameters and viability were higher (P < 0.05) for slow FR than fast FR samples. In Study 2 investigating the effects of liquid storage and sorting on sperm quality, motility and SMI after sorting and centrifugation were lower (P < 0.05) than those of the initial ejaculate. The sort rate for enrichment (91 ± 4% purity) of X- and Y-bearing spermatozoa was 3400 ± 850 spermatozoa sex−1 s−1. In Study 3, compared with a modified straw method, directional freezing resulted in enhanced in vitro quality of sorted and non-sorted spermatozoa derived from liquid-stored semen (P < 0.05). In Study 4, endoscopic insemination of three dolphins with sorted, frozen–thawed X-bearing spermatozoa resulted in one conception and the birth of a female calf. High-purity sorting of dolphin spermatozoa, derived from liquid-stored semen, can be achieved with minimal loss of in vitro sperm quality and samples are functional in vivo.

scholarly journals Evaluation of sperm quality of Erythrolamprus poecilogyrus sublineatus (Cope, 1860) (Serpentes, Dipsadidae)

2017 ◽  
Vol 77 (3) ◽  
pp. 553-557
Author(s):  
A. C. Silva ◽  
A. S. Varela Junior ◽  
T. F. Cardoso ◽  
E. F. Silva ◽  
D. Loebmann ◽  
...  
Keyword(s):  
Sperm Quality ◽  
Membrane Integrity ◽  
Coastal Region ◽  
Rio Grande ◽  
Rio Grande Do Sul ◽  
Sperm Analysis ◽  
Pampa Region

Abstract Erythrolamprus poecilogyrus sublineatus (Cope, 1860), is a species widely distributed in the Pampa Domain, occurring in Rio Grande do Sul, Argentina and Uruguay, mainlyin the pampa region. In the coastal region of southern Brazil this is serpent is considered one of the most abundant. The purpose of the present study is to describe the techniques of sperm evaluation in vitro for E. poecilogyrus sublineatus in the coastal plain of Rio Grande do Sul, Brazil. After laparatomy the efferent vases were collected and the semen was diluted in 1ml Beltsville Thawing Solution. The characteristics of motility, membrane integrity, mitochondria, acrosome, DNA, cell viability and cellular functionality were evaluated. Fluorescent probes were used for the evaluation of sperm structure in epifluorescence microscope. With the techniques described, it was possible to identify intact and injured cells, enabling the determination of cell characteristics for the spring season (October and November). It was observed in the analyses that 80% of sperm cells were mobile and that 84.1 ± 8.0% of sperm membranes were intact. The standards found were of 48 ± 13.8% of intact acrosome, 73.6 ± 6.0 of perfect DNA and of 91.8 ± 4.0 of functional mitochondria. Thus, these values from the sperm analysis can be used as standards for the species Erythrolamprus poecilogyrus sublineatus.

scholarly journals Oxytocin in pig seminal plasma is positively related with in vivo fertility of inseminated sows

2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Lorena Padilla ◽  
Marina López-Arjona ◽  
Silvia Martinez-Subiela ◽  
Heriberto Rodriguez-Martinez ◽  
Jordi Roca ◽  
...  
Keyword(s):  
Seminal Plasma ◽  
Sperm Quality ◽  
Sperm Count ◽  
Sperm Concentration ◽  
Reproductive Function ◽  
Peptide Hormone ◽  
Large White ◽  
Male Reproductive Function ◽  
Farrowing Rate

Abstract Background Identification of relevant in vivo biomarkers for fertility remains a challenge for the livestock industry. Concentrations of the small peptide hormone oxytocin (OXT), involved in male reproductive function and present in the seminal plasma (SP) of several species could be a robust one. This study characterized concentrations of SP-OXT in ejaculates from boars used in artificial insemination (AI) programs aiming to evaluate its relationship with sperm quality variables and in vivo fertility of their liquid-stored AI-semen. Seminal OXT concentrations (ng/mL) were measured in 169 ejaculates from 61 boars of the Duroc, Pietrain, Landrace and Large White breeds using a direct competitive immunoassay test based on AlphaLISA® technology. Ejaculate (ejaculate volume, sperm concentration, total sperm count) and sperm parameters (motility, viability, intracellular generation of reactive oxygen species, plasma membrane fluidity) were assessed at 0 h and 72 h in AI-semen samples stored at 17 °C. In vivo fertility included only 18 Large White and Landrace boars whose AI-semen was used to inseminated > 100 sows and evaluated both farrowing rate and litter size of 3,167 sows. Results The results showed that SP-OXT differed between boars and between ejaculates within boar (P < 0.05) but not between breeds (Duroc, Pietrain, Landrace and Large White). Ejaculates with higher SP-OXT concentration/mL (hierarchically grouped; P < 0.001) had larger volume and came from younger boars (P < 0.05). Ejaculates of boars showing positive farrowing rate deviation exhibited higher (P < 0.05) SP-OXT concentration/mL than those with negative farrowing rate deviation. Conclusion The SP concentrations of OXT are boar, ejaculate and age dependent, and positively related with ejaculate volume and farrowing rates of liquid-stored semen AI-doses.

scholarly journals Biological Effects of Non-ionizing Electromagnetic Fields to 27 GHz on Sperm Quality of Mytilus galloprovincialis

2022 ◽  
Author(s):  
Roberta Pecoraro ◽  
Santi Concetto Pavone ◽  
Elena Maria Scalisi ◽  
Carmen Sica ◽  
Sara Ignoto ◽  
...  
Keyword(s):  
Electromagnetic Fields ◽  
Mytilus Galloprovincialis ◽  
Sperm Quality ◽  
Biological Effects ◽  
Ecological Impact ◽  
Horn Antenna ◽  
High Gain ◽  
Wireless Internet

Recently, a rising use of wireless internet technologies has been demonstrated. The devices which use these technologies emit a considerable amount of electromagnetic radiation (EMFs) which could interact with the male reproductive system. The aim of this study was to investigate in vitro influence of electromagnetic fields a 27 GHz on sperm quality in Mytilus galloprovincialis. The experiments were conducted with a no commercial high gain pyramidal horn antenna. Sperm samples, taken from sexually mature males of M. galloprovincialis, were placed in seawater. Once evaluated the number and quality of spermatozoa, sperm cells were exposed to electromagnetic fields. The effect of exposure was evaluated after 10, 20, 30 and 40 minutes with light microscope and using Eosin test. All the samples were performed in triplicate and statistical analysis was carried out by one-way ANOVA test. A significative decrease (30%) in sperm motility was ob-served after 10 minutes of exposure and after 30 minutes all sperms were immobile and not vital. The measurement of the Oxidation Reduction Potential (sORP), which evaluates the oxidative damage on spermatozoa, shows how the exposure to 27 GHz has increased the oxidant amount compared to the control groups. This study provides useful data on potential ecological impact of the EMFs on aquatic animals, that currently are poor investigated.

Diagnosis and clinical criteria for chronic endometritis

GYNECOLOGY ◽  
2018 ◽  
Vol 20 (6) ◽  
pp. 53-59
Author(s):  
K G Serebrennikova ◽  
N A Arutyunyan ◽  
A I Alekhin
Keyword(s):  
Reproductive Function ◽  
Antiviral Effect ◽  
Chronic Endometritis ◽  
Vitro Fertilization ◽  
Clinical Criteria ◽  
New Methods ◽  
And Function ◽  
Social Importance

Currently, the urgency of the problem of chronic endometritis is of medical and social importance, since it leads to impaired reproductive function. Among the risk factors there is a predominance of intrauterine devices and inflammatory complications after childbirth and abortion. Prolonged persistence of infectious agents in the endometrium in chronic endometritis leads to marked changes in the structure and function of the tissue, causes proliferative disorders, as well as disorders in cyclic transformation. Morphofunctional damage to the endometrium is not only the cause of infertility, unsuccessful attempts of in vitro fertilization (IVF) and embryo transfer (PE), miscarriage, and a decrease in the quality of life of women due to chronic pain, dyspareunia, uterine bleeding and discharge from the genital tract. Timely and accurate diagnosis is key to the success of the restoration of reproductive function. Treatment of chronic endometritis is a complex, phased process. One of the new methods is photodynamic therapy, which leads to the development of a photochemical reaction that destroys bacterial and tumor cells, and also has an antiviral effect.

Levels of seminal tRNA-derived fragments from normozoospermic men correlate with the success rate of ART

2021 ◽  
Vol 27 (4) ◽  
Author(s):  
Julieta B Grosso ◽  
Luciana Zoff ◽  
Karina L Calvo ◽  
María Belén Maraval ◽  
Mariana Perez ◽  
...  
Keyword(s):  
Semen Quality ◽  
Physiological Stress ◽  
Sperm Quality ◽  
Semen Analysis ◽  
Reproductive Function ◽  
Medical Problem ◽  
Sperm Dna Fragmentation ◽  
Male Factor ◽  
Male Reproductive Function ◽  
A Prospective Study

Abstract Decreased fertility is becoming an important social and medical problem and the male factor is involved in at least half of infertility cases. Since conventional semen analysis provides limited prediction of male fertility; in this work, we evaluated the potential use of seminal small RNAs (sRNA) as markers of semen quality in ART. Our bioinformatic analyses of available sRNA-seq databases showed that the most abundant sRNA species in seminal plasma of normozoospermic men are tRNA-derived fragments (tRFs), a novel class of regulatory sRNAs. These molecules not only exert their function within cells but also are released into the extracellular environment where they could carry out signaling functions. To evaluate whether the assessment of seminal tRFs in normozoospermic men has a predictive value for the clinical outcome in ART, we performed a prospective study with couples who underwent ICSI cycles with donated oocytes. The results obtained demonstrated that levels of 5’tRF-Glu-CTC, 5’tRF-Lys-CTT, and 5’tRF-Gly-GCC are significantly elevated in seminal samples from cases with repeated failed ICSI cycles, suggesting a potential association between increased seminal tRFs and unexplained male infertility. Interestingly, these tRFs showed a negative association with seminal testosterone, highlighting their involvement in male endocrinology. Our findings also suggest that tRFs could play a role in modulating male reproductive function in response to physiological stress since they showed significant associations with the levels of sperm DNA fragmentation in couples that achieved pregnancy but not in cases with failed ICSI cycles where seminal cortisol levels correlate with sperm quality.

scholarly journals Book review: “It Starts with the Egg” by Rebecca Fett

2019 ◽  
Vol 13 (3) ◽  
pp. 255-260
Author(s):  
A. D. Makatsariya
Keyword(s):  
Polycystic Ovary ◽  
Recurrent Miscarriage ◽  
Action Plan ◽  
Reproductive Function ◽  
Poor Quality ◽  
Vitro Fertilization ◽  
Wide Range ◽  
Based Medicine

The licensed edition of Rebecca Fett's book, “It Starts with the Egg” has been published in Russian. Given its medical contents, it seems important to evaluate the information contained in the book from the perspective of a practicing obstetrician-gynecologist, focusing on the problem of miscarriage and pregravid preparation. Importantly, the book is based on evidence-based medicine: although the book is intended for a wide scope of readers, it contains a list of primary references similarly to that in peer-reviewed journals.The author analyzed and summarized the data from over 60 scientific articles. Although based on primary academic sources, the author used a simple and understandable language to explain the complex pathogenesis of infertility and the manipulations used in the assisted reproductive technology (ART).The book consists of 3 parts; each of them contains several chapters reviewing the causes of reproductive disorders and the ways to correct them. The first part addresses the reasons for poor quality of women’s eggs. The second part describes nutraceuticals and dietary supplements that can improve the quality of eggs. The third part of the book examines the diet, which helps improve this quality. A step-by-step action plan for improving reproductive function is also presented. The basic plan is suitable for those who are just thinking about pregnancy and those who have no reason to expect any difficulties, as well as for couples who have been struggling with infertility for several years. The mid-level plan is intended for those who already have some concerns about conceiving, but are not yet aware of personal problems with their reproductive function, as well as for women with polycystic ovary syndrome (PCOS) or irregular ovulation. The plan for those having serious problems is intended for women with recurrent miscarriage, as well as for couples with a decrease in the ovarian reserve who are trying to get pregnant using in vitro fertilization (IVF).Despite some remarks regarding the role of vitamin D and magnesium in pregravid preparation, the book contains a large number of useful and balanced recommendations that can improve the woman’s health and increase her chances to get pregnant and give birth to a healthy baby. Therefore, the book can be recommended for a wide range of readers.

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