Effect of Replacing Bone Ash with Fresh Water Snail (Pila Ampullacea) Shell Ash on Serum Biochemical Indices of Weaned Rabbits

Twenty five (25) mixed breed weaned rabbits were utilized in 84-days experiment to investigate the effect of replacing bone ash with fresh water snail (Pila ampullacea) shell ash on the serum biochemical composition of weaned rabbits. The experiment was conducted at the Teaching and Research Farm, University of Agriculture, Makurdi, Benue State, Nigeria for a period of 12 weeks. The rabbits were randomly assigned to the five dietary treatments and replicated five times giving a total of one rabbit per replicate in a completely randomized design (CRD). Rabbits were raised intensively in hutches of about 4ft × 4ft. Five experimental diets tagged T1 to T5 were formulated such that fresh water snail (Pila ampullacea) shell ash replaced bone ash at 0%, 25%, 50%, 75% and 100%. Feed and water were served ad libitum. Bio sanitary and bio security measures were strictly adhered to. At the end of the feeding trial, three rabbits per treatment were selected for the evaluation of serum biochemistry. The parameters assayed for were: total serum protein, serum globulin, serum albumin, cholesterol, serum calcium, and serum phosphorus. The study showed that the serum biochemical indices were not influenced (P˃0.05) by the dietary treatments except for calcium which was significantly (P<0.05) influenced, however, calcium values were within the normal reference values for rabbits. This study had shown that fresh water snail (Pila ampullacea) shell ash can serve as a substitute for bone ash in weaned rabbit diet up to 100% inclusion levels without adverse effect on the serum biochemical indices.

Effect of Replacing Bone Ash with Fresh Water Snail (Pila ampullacea) Shell Ash on the Performance of Weaned Rabbits

Twenty five weaned male rabbits of mixed breeds were used to investigate the effect of replacing bone ash with fresh water snail shell ash on the performance of weaned rabbits. The experiment was conducted at the Teaching and Research Farm, University of Agriculture, Makurdi, Benue State, Nigeria for a period of 12 weeks. The rabbits were randomly assigned to the five dietary treatments and replicated five times giving a total of one rabbit per replicate in a completely randomized design (CRD). Rabbits were raised intensively in hutches of about 4ft × 4ft. Five experimental diets tagged T1 to T5 were formulated such that fresh water snail shell ash replaced bone ash at 0%, 25%, 50%, 75% and 100%. Feed and water were served ad libitum. Bio sanitary and bio security measures were strictly adhered. The study showed that the growth parameters were not affected (P=.05) by the dietary treatments. This study had shown that fresh water snail shell ash can serve as a substitute for bone ash in weaned rabbits diet up to 100% without compromising their performances.

EFFECT OF CHICKEN BONE ASH ON MECHANICAL PROPERTIES OF Al 8011 REINFORCED WITH SILICON CARBIDE

To deal with demands in advanced engineering applications that focus on improved mechanical properties of metal composites, aluminium metal matrix composites are recommended. This paper deals with mechanical characteristics of aluminium (Al8011) fortified with silicon carbide (SiC) and Chicken Bone Ash (CBA).Varying percentages of silicon carbide starting from 0%, 2%, 4%, 6%, 8% and 10% with percentages of chicken bone ash starting from 10%, 8%, 6%, 4%, 2% and 0% respectively for the purpose of testing and reinforcement. Stir casting method is employed to manufacture the components. In this paper, fatigue test, impact test and hardness test is carried out to obtain results about the mechanical characteristics of the components. The mechanical characteristics observed in the resultant composites as per ASTM standards and microstructural study is used to characterize the distribution of reinforcements. Ultimately as the weight percentage of reinforcement materials increases, elevation in mechanical characteristics of material is discerned.

Tribological behaviour of lamb bone ash and boron carbide reinforced ZA-27 hybrid metal matrix composites under dry sliding conditions

This study aims to investigate the tribological behaviour of lamb bone ash (LBA) and boron carbide (B4C) reinforced ZA-27 hybrid metal matrix composites fabricated using a stir casting process. The weight percentage of LBA and B4C particles in the composites were varied from 0-5 wt.%. The composites have been evaluated for density, porosity and microhardness before tribological testing. Dry sliding friction and wear behaviour of composites were studied on a pin-on-disc tribometer by varying load from 10-50 N at a fixed sliding speed of 1 m/s. Also, to investigate the effect of sliding speed on friction and wear behaviour of composites, tests were carried out at 2 m/s and 3 m/s of sliding speed. A scanning electron microscope (SEM) was used for examining the microstructure and worn surface morphology of composite samples. SEM micrographs revealed the presence and homogeneous distribution of reinforcement particles, and energy-dispersive X-ray spectroscopy (EDS) analysis confirmed the presence of LBA and B4C particles in the composites. Composites density decreased, and porosity increased with the addition of reinforcement particles. The microhardness of the 5 wt.% reinforced LBA composite improved by 18.38%, whereas hybrid composite containing (2.5 wt.% LBA + 2.5 wt.% B4C) showed an improvement of 42% compared to the base alloy. The coefficient of friction (COF) and wear loss increased with the increase in load, whereas COF decreased and wear loss increased with the increase in sliding speed. Composites showed superior wear resistance even at higher loads and sliding speeds. SEM micrographs of worn surface revealed adhesion and abrasion type of wear mechanisms. Therefore, with the improvement in wear resistance this developed composite can be used as a bearing material over monolithic ZA-27 alloy in the automotive sector.

Increased microbial phytase increased phytate destruction, plasma inositol, and feed efficiency of weanling pigs, but reduced dietary calcium and phosphorus did not affect gastric pH or fecal score and reduced growth performance and bone ash

An experiment was conducted to test 2 hypotheses: 1) reducing dietary Ca and P reduces gastric pH and diarrhea in weanling pigs; 2) negative effects of low Ca and P on pig growth performance may be overcome if phytase is added to the diets. A total of 320 weanling pigs (6.35 ± 0.87 kg) were allotted to 8 corn-soybean meal-based diets in a randomized complete block design with 5 pigs per pen. Two phase 1 (d 1 to 14) control diets containing 100 or 50% of total Ca and digestible P relative to the requirement, and 6 diets in which 500, 2,000, or 16,000 units of phytase/kg feed (FTU) were added to each control diet were formulated. Phytase was assumed to release 0.16% total Ca and 0.11% digestible P. Common diets were fed in phases 2 (d 15 to 27) and 3 (d 28 to 42). Growth performance data were recorded within each phase. Data for fecal scores and gastrointestinal pH were recorded for phase 1. Colon content (d 14), the right femur (d 14 and 42), and blood samples (d -1, 14, 27, and 42) were collected from 1 pig per pen. In phase 1, reducing Ca and P did not reduce gastric pH or fecal score, but pigs fed the 50% diets had reduced (P &lt; 0.05) average daily gain (ADG) and average daily feed intake (ADFI) compared with pigs fed the 100% diets. In both 50 and 100% diets, phytase above 500 FTU increased (P &lt; 0.05) gain:feed ratio (G:F) and tended (P &lt; 0.10) to reduce gastric pH of pigs. From d 1 to 42, pigs fed the 50% diets tended (P &lt; 0.10) to have reduced ADG and ADFI compared with pigs fed the 100% diets, but among the 100% diets, pigs tended (P &lt; 0.10) to have a linear increase in G:F as phytase level increased. Pigs fed the 50% diets had reduced (P &lt; 0.05) concentrations of inositol phosphate esters (IP) in the colon and reduced bone ash (d 14 and 42) compared with pigs fed the 100% diets. Phytase did not affect bone ash or most blood metabolites. Concentrations of IP in the colon decreased, whereas plasma inositol increased (d 14; P &lt; 0.05) in pigs fed diets with phytase (≥ 500 FTU). In pigs fed the 100% diets, IP in the colon linearly decreased (P &lt; 0.05), but plasma inositol linearly increased (P &lt; 0.05) with increasing levels of phytase. In conclusion, reducing Ca and P in diets for weanling pigs did not influence gastric pH or fecal score, but compromised growth performance and bone ash. However, regardless of dietary Ca and P, high doses of phytase increased phytate degradation and inositol absorption, which consequently increased G:F of pigs.

474 Comparison Between Copper Sulfate and Dicopper Oxide on Body Weight and Copper Accumulation on Growing Pigs

There are several Cu sources in the market that have different effects on pigs. We hypothesized that supplementing therapeutic doses of Cu(I)oxide (Cu2O) in diets for growing pigs improves body weight (BW), bone mineralization, and reduces Cu accumulation in liver than supplementing pigs with Cu sulfate (CuSO4). Two-hundred growing pigs (initial BW: 11.5 ± 0.98 kg) were randomly allotted to 5 dietary treatments. Dietary treatments included the negative control (NC) diet without Cu supplementation, and 4 diets in which 125 or 250 mg/kg of Cu from CuSO4 or Cu2O were added to the NC diet. The experiment was divided into 4 phases. Pig weights were recorded on d 1 and at the end of each phase. Eight pigs per treatment were sacrificed last d of phases 1 and 4 to obtain samples of liver, spleen, and the right metacarpal. Pigs fed diets containing 250 mg/kg of Cu2O had greater (P &lt; 0.05) BW at the end of phases 1 and 2 than pigs fed the NC diet, and tended (P &lt; 0.10) to have greater BW at the end of phases 3 and 4 than pigs fed the other diets. Copper accumulation in liver and spleen increased with Cu dose, but at the end of phase 1, pigs fed 250 mg/kg CuSO4 had greater (P &lt; 0.05) Cu concentration in liver and spleen than pigs fed 250 mg/kg Cu2O. Pigs fed diets containing 250 mg/kg Cu2O had greater (P &lt; 0.05) quantities of bone ash and greater (P &lt; 0.05) concentrations of Ca, P, and Cu in bone ash than pigs fed the NC diets or diets containing CuSO4. Therefore, supplementing diets for growing pigs with therapeutic doses of Cu2O improves BW and bone mineralization with less Cu accumulation in liver compared with pigs fed diets containing CuSO4.

The Effect of Animal Bone Ash on the Mechanical Properties of Asphalt Concrete

For the sake of enhancing the mechanical properties and durability of asphalt concrete, many studies suggest adding different admixtures, such as waste materials in the form of filler. These admixtures have a significant influence on the performance of asphalt concrete by plying a roll in filling the voids between particles and sometimes as a cementitious material. This study aims to improve the strength of asphalt concrete by adding crushed animal bone to the mix after carbonization at a temperature of 800 Co. Seven different percentages (10, 20, 30, 40, 50, 60, and 100%) of animal bone ash as a replacement for the filler percentage were added to the optimum asphalt concrete mix. A number of tests were conducted on asphalt concrete specimens to measure Marshall stability (MS), Marshall flow value (MF), voids filled with asphalt percentages (VFA), air void percentages (VA), voids in mineral aggregate percentages (VMA), and maximum theoretical specific gravity (GMM). From the results, the maximum stability of 14.85 KN was reached when using animal bone ash of 20% as a partial replacement for the conventionally used filler (limestone). In general, there are some improvements in the physical properties of asphalt concrete with animal bone ash, which can be related to the increase in the bond between the particles of aggregates and the bitumen material. Doi: 10.28991/cej-2021-03091757 Full Text: PDF

Dietary calcium requirements of broilers fed a conventional corn-soybean meal diet from 1 to 21 d of age

Background The current calcium (Ca) recommendation for broilers is primarily based on studies conducted more than 30 years ago with birds of markedly different productive potentials from those which exist today. And the response indicators in these studies are mainly growth performance and bone ash percentage. Therefore, the present study was carried out to investigate the effect of dietary Ca level on growth performance, serum parameters, bone characteristics and Ca metabolism-related gene expressions, so as to estimate dietary Ca requirements of broilers fed a conventional corn-soybean meal diet from 1 to 21 d of age. Methods A total of 420 1-day-old Arbor Acres male broilers were randomly assigned to 1 of 7 treatments with 6 replicates (10 birds per cage) and fed the corn-soybean meal diets containing 0.60, 0.70, 0.80, 0.90, 1.00, 1.10 or 1.20% Ca for 21days. Each diet contained the constant non-phytate phosphorus content of about 0.39%. Results The average daily gain decreased linearly (P < 0.001) as dietary Ca level increased. The serum and tibia alkaline phosphatase (ALP) activities, tibia bone mineral density (BMD), middle toe BMD, tibia ash percentage, tibia breaking strength, and tibia ALP protein expression level were affected (P < 0.05) by dietary Ca level, and showed significant quadratic responses (P < 0.02) to dietary Ca levels. The estimates of dietary Ca requirements were 0.80 to 1.00% based on the best fitted broken-line or quadratic models (P < 0.03) of the above serum and bone parameters, respectively. Conclusions The results from the present study indicate that the Ca requirements would be about 0.60% to obtain the best growth rate, and 1.00% to meet all of the Ca metabolisms and bone development of broilers fed a conventional corn-soybean meal diet from 1 to 21 days of age.

The most balance composition of calcium-phosphorus in the feed to support growth performance and tibia profile of broiler chicken strain CP 707

<p class="MDPI17abstract"><strong>Objective: </strong>Calcium and phosphorus are macrominerals that are needed for metabolism and mineralization of chicken bones. This study aims to determine the growth performance and tibial profile of broiler chicken strain CP 707 which are fed by using different calcium-phosphorus balance compositions.</p><p class="MDPI17abstract"><strong>Methods: </strong>This research was conducted in Kefamenanu, the East Nusa Tenggara for 42 days. A total of 96 day old chicks were used in this study. Chickens were allocated in 16 units of cages, based on a completely randomized design consisting of 4 treatments and 4 replications. The treatments given were T0 (control feed without calcium-phosphorus supplementation), T1 (control feed + 1.0% calcium + 0.5% phosphorus), T2 (control feed + 1.5% calcium + 0.5% phosphorus) and T3 (control feed + 2.0% calcium + 0.5% phosphorus).<strong></strong></p><p class="MDPI17abstract"><strong>Results</strong><strong>: </strong>The findings showed that the calcium-phosphorus ratio affected weight gain, feed conversion, carcass weight, tibial bone weight, tibial bone dry weight and tibial bone ash content (P&lt;0.05), while feed consumption and tibial bone length were not significant. The highest body weight was in T3 (2142.25±31.84 g/bird) with a weight gain of 62.41±2.20 g/bird/day. The best feed conversions at T2 and T3 were 1.94±0.33 and 1.85±0.70. The highest carcass weight at T3 was 1354.45±39.12 g/bird with a carcass percentage of 63.20±0.32%. The highest tibial weight at T2 and T3 were 18.57±0.73 and 19.43±0.66 g/bird with dry weight of 40.61±0.75 and 41.42±1.33%. The highest tibial bone ash content was at T3, namely 44.72±0.91%.<strong></strong></p><strong>Conclusions: </strong>The conclusion of this study is that the balance of 2.0% calcium and 0.5% phosphorus provides optimal growth performance and tibial profile.<p align="center"> </p>

Influence of a novel consensus bacterial 6-phytase variant on mineral digestibility and bone ash in young growing pigs fed diets with different concentrations of phytate-bound phosphorus

A 20-d experiment was conducted to test the hypothesis that phytase increases nutrient digestibility, bone ash, and growth performance of pigs fed diets containing 0.23%, 0.29%, or 0.35% phytate-bound P. Within each level of phytate, 5 diets were formulated to contain 0, 500, 1,000, 2,000, or 4,000 phytase units (FTU)/kg of a novel phytase (PhyG). Three reference diets were formulated by adding a commercial Buttiauxella phytase (PhyB) at 1,000 FTU/kg to diets containing 0.23, 0.29, or 0.35% phytate-bound P. A randomized complete block design with 144 individually-housed pigs (12.70 ± 4.01 kg), 18 diets, and 8 replicate pigs per diet was used. Pigs were adapted to diets for 15 d followed by 4 days of fecal collection. Femurs were collected on the last day of the experiment. Results indicated that diets containing 0.35% phytate-bound P had reduced (P &lt; 0.01) digestibility of Ca, P, Mg, and K compared with diets containing less phytate-bound P. Due to increased concentration of total P in diets with high phytate, concentrations, apparent total tract digestible P and bone ash were increased by PhyG to a greater extent in diets with 0.29 or 0.35% phytate-bound P than in diets with 0.23% phytate-bound P (interaction, P &lt; 0.05). At 1,000 FTU/kg, PhyG increased P digestibility and bone P more (P &lt; 0.05) than PhyB. The novel phytase increased (P &lt; 0.01) pig growth performance, and pigs fed diets containing 0.35 or 0.29% phytate-bound P performed better (P &lt; 0.01) than pigs fed the 0.23% phytate-bound P diets. In conclusion, the novel phytase (i.e., PhyG) is effective in increasing bone ash, mineral digestibility, and growth performance of pigs regardless of dietary phytate level.