Protein quality evaluation in vivo of cricket flour (Gryllus assimilis) reared in Brazil

This study aimed to evaluate the chemical and biological quality of Gryllus assimilis flour, through the analysis of nutritional and biochemical parameters of tests on young rats (Rattus norvegicus – Fischer). The most deficient essential amino acid was methionine, with a chemical score of 56.40%. For the net protein ratio (NPR) and net protein utilisation (NPU) ratio, the diet containing unsupplemented cricket flour was not enough to reach the NPR and NPU levels of the control group and was insufficient to promote weight gain in the rats. The addition of methionine and wheat bran increased the digestibility of the cricket flour, which resulted in a significant improvement in the levels of cricket protein utilisation, increasing the rates mentioned above and promoting weight gain in the rats. Based on our results, we can state that considering the age of the study animals, cricket meal was a sufficient source of protein for maintenance of body weight, but not for weight gain. Based on biochemical parameters (total protein, albumin, alanine transaminase, aspartate transaminase, creatinine, urea), the cricket meal did not compromise the general health of the study animals. We therefore conclude that the addition of other sources of methionine to cricket flour can be a fundamental strategy for better use of cricket proteins in the diet.

Dietary valine levels affect growth, protein utilisation, immunity and antioxidant status in juvenile hybrid grouper (Epinephelus fuscoguttatus ♀ × Epinephelus lanceolatus ♂)

A 6-week growth trial was conducted to evaluate the influences of dietary valine (Val) levels on growth, protein utilisation, immunity, antioxidant status and gut micromorphology of juvenile hybrid groupers. Seven isoenergetic, isoproteic and isolipidic diets were formulated to contain graded Val levels (1·21, 1·32, 1·45, 1·58, 1·69, 1·82 and 1·94 %, DM basis). Each experimental diet was hand-fed to triplicate groups of twelve hybrid grouper juveniles. Results showed that weight gain percentage (WG%), protein productive value, protein efficiency ratio, and feed efficiency were increased as dietary Val level increased, reaching a peak value at 1·58 % dietary Val. The quadratic regression analysis of WG% against dietary Val levels indicated that the optimum dietary Val requirement for hybrid groupers was estimated to be 1·56 %. Gut micromorphology and expression of growth hormone in pituitary, insulin-like growth factor 1, target of rapamycin and S6 kinase 1 in liver were significantly affected by dietary Val levels. In serum, fish fed 1·58 % dietary Val had higher superoxide dismutase, catalase, lysozyme activities and IgM concentrations than fish fed other dietary Val levels. Fish fed 1·58 % dietary Val had higher expression of NF-E2-related factor 2 in head kidney than fish fed other dietary Val levels. Generally, the optimum dietary Val requirement for maximal growth of hybrid groupers was estimated to be 1·56 % of DM, corresponding to 3·16 % of dietary protein, and dietary Val levels affected growth, protein utilisation, immunity and antioxidant status in hybrid groupers.

Efficiency of Crude Protein Utilisation in Grazing Dairy Cows: A Case Study Comparing Two Production Systems Differing in Intensification Level in New Zealand

In this study, we modelled and compared lactation curves of efficiency of crude protein utilisation (ECPU) and the nitrogen (N) excreta partitioning of milking cows of two contrasting spring-calving pasture-based herds to test some aspects of farming intensification practices on cow performance and N partition. In the low-intensity production system (LIPS), 257 cows were milked once-daily and fed diets comprised of pasture with low supplementary feed inclusion during lactation (304 kg pasture silage/cow). In the high-intensity production system (HIPS), 207 cows were milked twice-daily and fed pasture with higher supplementary feed inclusion (429 kg pasture silage and 1695 kg concentrate/cow). The dietary crude protein (CP) utilisation was calculated for each cow at every herd test date as the ECPU as a proportion of protein yield (PY) from the CP intake (CPI) derived from intake assessments based on metabolisable energy requirements, and the CP balance (CPB) calculated as the difference between CPI and PY. Total N excreta partitioned to faeces (FN) and urine (UN) was estimated by back-calculating UN from FN, considering dietary N, and from N retained in body tissues, taking into account live weight change during the lactation. The higher CPI (2.7 vs. 2.5 kg CP/day), along with the reduced milk yield (1100 kg milk/cow less), of the LIPS cows led to a lower ECPU (23% vs. 31%) and to a higher CPB (2.1 vs. 1.8 kg CP/day) when compared to the HIPS cows. Mean N excreta, and particularly UN, was significantly higher in LIPS cows, and this was explained by higher dietary CP and by the reduced PY when compared to the HIPS cows. Reducing the low-CP supplementation in the “de-intensified” herd lessened the ECPU, resulting in higher UN, which is sensitive in terms of body water eutrophication.

Dietary intake of cod protein beneficially affects concentrations of urinary markers of kidney function and results in lower urinary loss of amino acids in obese Zucker fa/fa rats

Obesity increases the risk for developing kidney disease, and protection of kidneys through changes in diet should be investigated. Fish intake has been associated with reduced risk of developing kidney disease; therefore, we wanted to investigate whether cod protein intake could prevent or delay the development of kidney damage in an obese rat model that spontaneously develops proteinuria and focal segmental glomerulosclerosis. The aim of the study was to investigate any effects of cod protein intake on established markers of kidney function, amino acid composition, protein utilisation and growth in obese Zucker fa/fa rats in the early stage of decreased renal function. Male obese Zucker fa/fa rats (HsdOla:Zucker-Lepr) were fed cod muscle proteins in an amount corresponding to 25 % of dietary protein, with the remaining protein from a casein/whey mixture (COD diet). A control group was fed a diet with a casein/whey mixture as the only protein source (CAS diet). The intervention started when rats were 9–10 weeks old, and the rats were fed these diets for 4 weeks. At the end of the study, rats fed the COD diet had lower urine concentration of cystatin C, T-cell immunoglobulin mucin-1 (TIM-1), amino acids, carbamide, uric acid and ammonium and higher concentrations of creatine, trimethylamine N-oxide, 1-methylhistidine and 3-methylhistidine, lower kidney concentration of TIM-1 and showed better growth when compared with the CAS group. To conclude, cod protein may have the potential to delay the development of kidney damage in young obese Zucker rats and to improve protein utilisation and growth.