RIT1 Promotes Glioma Proliferation and Invasion via the NF-ĸB/p65 pathway

RIT1, a member of the Ras family, has been identified as an oncogene in several malignancies. However, the expression and function of RIT1 in glioma remains to be addressed. In this study, we found RIT1 was upregulated in glioma and was associated with poor prognosis of glioma patients. Manipulating RIT1 levels in glioma cells via RNA interference significantly inhibited glioma cell proliferation and invasion in vitro whereas RIT1 overexpression exhibited the opposite effects. Mechanistically, we demonstrate that RIT1 engaged in the activation of the NF-ĸB pathway in vitro and in vivo. Furthermore, treating RIT1-overexpressing glioma cells with the p65 siRNA partially restrained their proliferation and invasion. Together these results indicate RIT1 contributes to the development and metastasis of glioma via the NF-ĸB pathway and suggest that targeting RIT1 may be a treatment strategy for this disease.

User Needs and Limitations of Existing Mobility Devices

The use of gait assistive devices, although can provide precious help can also have limitations or adverse consequences. The performance of daily activities is partially restrained by a series of factors having to do with the assistive device limitations or the limitations emerged from environmental causes. Valid and reliable assessment tools to classify and clarify their limitations or users' satisfaction do not exist. An extended literature search was performed for some of the most commonly mentioned assistive device limitations or dissatisfaction reasons and identified the most appropriate tools to be able to study such limitations and conclude in valid outcomes. A study was conducted in a rehabilitation hospital using the IPA, EQ5D and SF-12 tools. The size of the sample combined with the uncertainty related to the satisfaction of normal distribution prerequisite, led to use several statistical analysis tools and methodologies. A summary of the results from both the literature review and the real case study on a mixed group of mobility aid users are presented in the paper.

Stochastic Finite Element Analysis of Composite Structures with Partially Restrained Connections under Earthquake Loads

The stochastic analysis of composite structures with partially restrained (PR) connections under seismic loads present some interesting and challenging issues to practicing engineers. This paper proposes an efficient, robust, and accurate method for stochastic finite element analysis of concrete–steel composite structures allowing for PR connections. These are followed by suitable numerical example which indicates that employment of such a stochastic finite element analysis. The Kocaeli earthquake in 1999 is considered as a ground motion. The connections parameters and material properties are random variables. It is essential to properly consider the PR connections in the stochastic dynamic analysis and design of the steel-concrete composite structures since design forces change significantly. The assumption that the connections are rigid, which is routinely used in the application, is not proper. The effect of the variability connection stiffness on the composite structures responses is sufficiently important for consideration in structural safety.

Bidirectional Response of Weak-Axis End-plate Moment Connections: Numerical Approach

Brittle failure mechanisms can affect the seismic performance of structures composed of intersecting moment resisting frames, if the biaxial effects are not considered. In this research, the bidirectional cyclic response of H-columns with weak-axis moment connections was studied using numerical models. Several configurations of joints with bidirectional effects and variable axial loads were studied using the finite element method (FEM) in ANSYS v17.2 software. The results obtained showed a ductile behavior when cyclic loads are applied. No evidence of brittle failure mechanisms in the studied joint configurations was observed, in line with the design philosophy established in current seismic provisions. However, beams connected to the column minor axis reached a partially restrained behavior. Joints with four beams connected to the column exhibited a partially restrained behavior for all axial load levels. An equivalent force displacement method was used to compare the hysteretic response of 2D and 3D joints, obtaining higher deformations in 3D joints with respect to 2D joints with a similar number of connected beams. Consequently, design procedures are not capable of capturing the 3D deformation phenomenon.