HY5: A Pivotal Regulator of Light-Dependent Development in Higher Plants

ELONGATED HYPOCOTYL5 (HY5), a bZIP-type transcription factor, acts as a master regulator that regulates various physiological and biological processes in plants such as photomorphogenesis, root growth, flavonoid biosynthesis and accumulation, nutrient acquisition, and response to abiotic stresses. HY5 is evolutionally conserved in function among various plant species. HY5 acts as a master regulator of light-mediated transcriptional regulatory hub that directly or indirectly controls the transcription of approximately one-third of genes at the whole genome level. The transcription, protein abundance, and activity of HY5 are tightly modulated by a variety of factors through distinct regulatory mechanisms. This review primarily summarizes recent advances on HY5-mediated molecular and physiological processes and regulatory mechanisms on HY5 in the model plant Arabidopsis as well as in crops.

The false promise of global IR: exposing the paradox of dependent development

Concerned about the continued dominance of Western International Relations (IR) theories, the global IR community has proposed various measures to address disciplinary hierarchies through encouraging dialogue and pluralism. By investigating the pedagogical preferences of instructors from 45 countries, this paper questions the global IR initiative's emancipatory potential, arguing that disciplinary practices in IR resemble those of dependent development. The study develops a new typology of IR theoretical (IRT) scholarship and examines the readings assigned in 151 IRT syllabi worldwide for evidence of similarity, replication, and assimilation. The findings show that mainstream core IRTs dominate syllabi globally, regardless of region, language of instruction, or instructors' educational/linguistic backgrounds. This domination extends to periphery scholars not using their own local products. Even when they do seek alternative approaches, they prefer to import core alternatives, that is, critical traditions, rather than homegrown IRTs. Finally, the results show that even in syllabi taught in local languages the readings remain dominated by core IRT works. These findings expose a structural defect in the current cry for global IR, by revealing the system's dependent development paradox. The paper concludes with suggestions for creating a symmetric interdependent structure, in the aim of achieving a genuine globalization of IR.

The divergent logics of urban regeneration in Israel: A neoliberal toolkit and national rationales

In recent years, urban regeneration policy in Israel has relied largely on market-based mechanisms to deliver its goals, seemingly in keeping with neoliberal trends. Whereas, in previous decades, the construction and renovation of housing was facilitated primarily by state-run projects, current urban regeneration policy relies heavily on private actors – developers and homeowners – motivated by profit and the allocation of building rights. In this article, we argue that while this policy appears to be consistent with neoliberal trends, the Israeli government, as well as the public, in fact continue to view urban regeneration as a project of national significance, deserving of public funding if market forces should prove inadequate. We describe the unique characteristics of urban regeneration policy in Israel, arguing that they derive from ‘moral economy’ logic as well as geopolitical considerations such as national security and commitment to the periphery. We make this argument by examining urban regeneration in the country’s geographical and economic ‘periphery’, where the state is expected to finance and incentivise regeneration in the absence of market incentives. We conclude that even in a supposedly heightened neoliberal era, Israel’s regeneration policy continues to be centralised and driven by national objectives and centre–periphery relations that reproduce the country’s path-dependent development trajectory.

Sex-dependent development of Kras-induced anal squamous cell carcinoma in mice

Anal squamous cell carcinoma (SCC) will be diagnosed in an estimated 9,080 adults in the United States this year, and rates have been rising over the last several decades. Most people that develop anal SCC have associated human papillomavirus (HPV) infection (~85-95%), with approximately 5-15% of anal SCC cases occurring in HPV-negative patients from unknown etiology. This study identified and characterized the Kras-driven, female sex hormone-dependent development of anal squamous cell carcinoma (SCC) in the LSL-KrasG12D ; Pdx1-Cre (KC) mouse model that is not dependent on papillomavirus infection. One hundred percent of female KC mice develop anal SCC, while no male KC mice develop tumors. Both male and female KC anal tissue express Pdx1 and Cre-recombinase mRNA, and the activated mutant KrasG12D gene. Although the driver gene mutation KrasG12D is present in anus of both sexes, only female KC mice develop Kras-mutant induced anal SCC. To understand the sex-dependent differences, KC male mice were castrated and KC female mice were ovariectomized. Castrated KC males displayed an unchanged phenotype with no anal tumor formation. In contrast, ovariectomized KC females demonstrated a marked reduction in anal SCC development, with only 15% developing anal SCC. Finally, exogenous administration of estrogen rescued the tumor development in ovariectomized KC female mice and induced tumor development in castrated KC males. These results confirm that the anal SCC is estrogen mediated. The delineation of the role of female sex hormones in mediating mutant Kras to drive anal SCC pathogenesis highlights a subtype of anal SCC that is independent of papillomavirus infection. These findings may have clinical applicability for the papillomavirus-negative subset of anal SCC patients that typically respond poorly to standard of care chemoradiation.

A lipid-mTORC1 nutrient-sensing pathway regulates animal development by peroxisome-derived hormones

Animals have developed many signaling mechanisms that alter cellular and developmental programs in response to changes in nutrients and their derived metabolites, many of which remain to be understood. We recently uncovered that glucosylceramides, a core sphingolipid, act as a critical nutrient signal for overall amino-acid level to promote development by activating the intestinal mTORC1 pathway. However, how the intestinal GlcCer-mTORC1 activity regulates development throughout the whole body is unknown. Through a large-scale genetic screen, we found that the peroxisomes are critical for antagonizing the GlcCer-mTORC1-mediated nutrient signal. Mechanistically, deficiency of glucosylceramide, inactivation of the downstream mTORC1 activity, or prolonged starvation relocated peroxisomes closer to the intestinal apical region to release peroxisomal-beta-oxidation derived hormones that targeting chemosensory neurons to arrest the animal development. Our data illustrated a new gut-brain axis for orchestrating nutrient-sensing dependent development in Caenorhabditis elegans, which may also explain why glucosylceramide and peroxisome become essential in metazoans.