In 1990, there was a "renewal" of local self-government in the Czech Republic
and other legislative changes took place that strengthened the powers of municipalities and allowed them to develop completely new forms and ways of cooperation, such as microregions, joint participation in business corporations and interest groups of legal entities (associations), etc. The activities of the Union of Towns and Municipalities of the Czech Republic were also renewed. The entire 1990s are marked by the emergence of new regional and national cooperation structures, with further acceleration coming in connection with the accession of the Czech Republic to the EU in 2004. Late 2010s are connected with strengthening relations between the private and public sectors, or with the division of existing forms of cooperation, due to which municipalities now have a fragmented structure of more than 20 possible ways of cooperation with other municipalities. However, in individual areas of the Czech Republic, this cooperation has different intensity, spatial arrangement and, of course, also different legal form, the historical genesis of which is mapped in this paper.