Biotransformation and oxidative stress responses in fish (Astyanax aeneus) inhabiting a Caribbean estuary contaminated with pesticide residues from agricultural runoff

The estuarine ecosystem of Laguna Madre de Dios (LMD), in the Caribbean coast of Costa Rica, is exposed to contamination with pesticide residues coming from the upstream agricultural areas. Biomarkers can provide a better indication of the fitness of biota in real mixture exposure scenarios than traditional lethal dose toxicity measurements. Here, we measured biomarkers of biotransformation, oxidative stress and neurotoxicity on Astyanax aeneus, an abundant fish species in LMD. Glutathione S-transferase activity (GST), catalase activity (CAT), lipid peroxidation (LPO), and cholinesterase activity (ChE) were measured in fish collected during seven sampling campaigns, carried out between 2016 and 2018. Pesticide residues were analysed in surface water samples collected every time fish were sampled. Residues of 25 pesticides, including fungicides, insecticides and herbicides, were detected. The biomarkers measured in A. aeneus varied along the sampling moments, however, biotransformation and oxidative stress signals showed coupled responses throughout the assessment. Furthermore, significant correlations were established between three biomarkers (GST, LPO and CAT) and individual pesticides, as well as between GST and LPO with groups of pesticides with shared biocide action. Among pesticides, insecticide residues had a major influence on the responses observed in fish. This work shows that the frequent exposure to environmentally relevant concentrations of pesticides can be related to physiological responses in fish that affect their health. This early warning information should be considered to improve the protection of estuarine ecosystems in the tropics.

Effectiveness of a Constructed Wetland with Carbon Filtration in Reducing Pesticides Associated with Agricultural Runoff

The Salinas Valley in Monterey County, California, USA, is a highly productive agricultural region. Irrigation runoff containing pesticides at concentrations toxic to aquatic organisms poses a threat to aquatic ecosystems within local watersheds. This study monitored the effectiveness of a constructed wetland treatment system with a granulated activated carbon (GAC) filter installation at reducing pesticide concentrations and associated toxicity to Ceriodaphnia dubia, Hyalella azteca, and Chironomus dilutus. The wetland was supplied with water pumped from an impaired agricultural and urban drainage. Across five monitoring trials, the integrated system’s average pesticide concentration reduction was 52%. The wetland channel and GAC filtration components individually provided significant treatment, and within each, pesticide solubility had a significant effect on changes in pesticide concentrations. The integrated treatment system also reduced nitrate by 61%, phosphate by 73%, and turbidity by 90%. Input water was significantly toxic to C. dubia and H. azteca in the first trial. Toxicity to C. dubia persisted throughout the system, whereas toxicity to H. azteca was removed by the channel, but there was residual toxicity post-GAC. The final trial had significant input toxicity to H. azteca and C. dilutus. The channel reduced toxicity to H. azteca and removed toxicity to C. dilutus. GAC filtration reduced H. azteca toxicity to an insignificant level. There was no input toxicity in the other three trials. The results demonstrate that a wetland treatment system coupled with GAC filtration can reduce pesticide concentrations, nutrients, suspended particles, and aquatic toxicity associated with agricultural runoff.

Sizing an open-channel woodchip bioreactor to treat nitrate from agricultural tile-drainage and achieve water quality targets

Woodchip bioreactors are capable of removing nitrate from agricultural runoff and subsurface tile drain water, alleviating human health hazards and harmful discharge to the environment. Water pumped from agricultural tile drain sumps to nearby ditches or channels could be cost effectively diverted through a woodchip bioreactor to remove nitrate prior to discharge into local waterways. Sizing the bioreactor to achieve targeted outlet concentrations within a minimum footprint is important to minimizing cost. Determining the necessary bioreactor size should involve a hydrological component as well as reaction type and rates. We measured inflow and outflow nitrate concentrations in a pumped open-channel woodchip bioreactor over a 13-month period and used a tanks-in-series approach to model hydrology and estimate parameter values for reaction kinetics. Both zero-order and first-order reaction kinetics incorporating the Arrhenius equation for temperature dependence were modeled. The zero-order model fit the data better. The rate coefficients (k = 17.5 g N m−3 day−1 and theta = 1.12 against Tref = 20 °C) can be used for estimating the size of a woodchip bioreactor to treat nitrate in agricultural runoff from farm blocks on California's central coast. We present an Excel model for our tanks-in-series hydrology to aid in estimating bioreactor size.

Water Quality Assessment with Biotic Index Based on Abundance and Diversity of Aquatic Insects in a Hilly Stream, Bangladesh

Study of stream insect fauna provide valuable insights into aspects of the stream channel ecosystem. The present study was conducted to investigate the aquatic stream living insect community, abundance and diversity in a hilly stream, Balukhali chora of Chittagong University campus to determine the water quality. The insects were collected with bottom dredge net from the edge and benthic regions of the Riffle zone and the Pool zone of the stream from January 2018 to December 2018. Insects were sampled using standard entomological method and determined their tolerance value. A total of 2535 insects were recorded, belonging to six insect orders, 30 families and 45 genera. The abundance ratio was higher in all the months in the Pool zone excepting the months of April, May, June and October. The orders Ephemeroptera, Odonata and Diptera were abundant in the Pool zone, while Hemiptera, Coleoptera and Lepidoptera were abundant in the Riffle zone. On the basis of Biotic Index, the most dominating orders Odonata and Hemiptera indicated good water quality, though the dipteran genus Chironomus spp. indicated poor quality in some of the months. The stream insect community structure of the two zones indicated that the overall water quality of the stream water was very good. Both manmade and natural interruption occurred in the stream channel due to human settlement, agricultural runoff and natural disasters. The study was conducted to know the abundance and diversity of aquatic insect community which indicated the water quality of the stream.

The relationship between maritime phosphate pollution and socioeconomic wellbeing

Phosphate rock reserves are expected to deplete in the next 50-100 years, with the point of highest phosphorus production predicted to be in 2030. Phosphate, the base of many fertilizers, is a non-renewable resource. Ocean phosphate concentrations provide a good indication of global fertilizer use, since agricultural runoff often contributes to increases in ocean phosphate concentration. This study explores the relationship between the concentration of phosphate in a nation’s maritime borders and the nation’s score on the Social Progress Index. The study aims to link findings with possible approaches to help meet two of the United Nations’ (UN) Sustainable Development Goals: creating sustainable communities, and conserving and sustainably using oceans. Phosphate concentration data were acquired from the National Oceanic and Atmospheric Administration and compared with factors of social welfare from the 2018 Social Progress Index. For each nation on the Social Progress Index, the nation’s score on every factor was separately compared to ocean phosphate concentration data within that nation’s maritime borders, and a linear regression was performed for each comparison. The results indicate countries ranking higher on the Social Progress Index generally have greater ocean phosphate concentrations, suggesting that countries of higher social welfare contribute more to global phosphate use or have greater amounts of fertilizer runoff. The findings should be considered by developed nations to inform decisions around pollution reduction as well as developing nations aiming for sustainable social progress. Both should consider the environmental effects that social progress has or will have on the greater global community, of which the significance to sustainable resource development and environmental protection is invaluable.

A Review on Constructed Treatment Wetlands for Removal of Pollutants in the Agricultural Runoff

Constructed wetland (CW) is a popular sustainable best management practice for treating different wastewaters. While there are many articles on the removal of pollutants from different wastewaters, a comprehensive and critical review on the removal of pollutants other than nutrients that occur in agricultural field runoff and wastewater from animal facilities, including pesticides, insecticides, veterinary medicine, and antimicrobial-resistant genes are currently unavailable. Consequently, this paper summarized recent findings on the occurrence of such pollutants in the agricultural runoff water, their removal by different wetlands (surface flow, subsurface horizontal flow, subsurface vertical flow, and hybrid), and removal mechanisms, and analyzed the factors that affect the removal. The information is then used to highlight the current research gaps and needs for resilient and sustainable treatment systems. Factors, including contaminant property, aeration, type, and design of CWs, hydraulic parameters, substrate medium, and vegetation, impact the removal performance of the CWs. Hydraulic loading of 10–30 cm/d and hydraulic retention of 6–8 days were found to be optimal for the removal of agricultural pollutants from wetlands. The pollutants in agricultural wastewater, excluding nutrients and sediment, and their treatment utilizing different nature-based solutions, such as wetlands, are understudied, implying the need for more of such studies. This study reinforced the notion that wetlands are effective for treating agricultural wastewater (removal >90%) but several research questions remain unanswered. More long-term research in the actual field utilizing environmentally relevant concentrations to seek actual impacts of weather, plants, substrates, hydrology, and other design parameters, such as aeration and layout of wetland cells on the removal of pollutants, are needed.

Heavy Metal Contamination and Human Health Implications in the Chan Thnal Reservoir, Cambodia

Chan Thnal reservoir, built during the Pol Pot period, is the major water source for the people in Krang Chek commune, Kampong Speu Province, Cambodia. Metal pollution caused by agricultural activities, improper wastewater treatment, and municipal waste disposal poses serious environmental health problems. In this study, the concentrations of four potential toxic metals (i.e., Cd, Cu, Pb, and Zn) from six locations across the reservoir were investigated both in the water and sediment. The results reflected progressive deterioration and indicated moderate to heavy pollution from the metals. The metal levels in the water were in the order of Zn > Cu > Pb > Cd. The statistical analysis revealed primary sources of heavy metals contamination in the water. Cd, Cu, Zn, and Pb in the water likely originate from anthropogenic activities including agricultural runoff (i.e., the use of fertilizers and pesticides) and urban runoff (i.e., improper wastewater discharge and waste disposal). Among the four metals, the Pb levels in the water significantly exceeded the guideline for drinking water in all locations. The health risk assessment revealed serious non-carcinogenic risks of Pb intake in the children at the age below 10 and infants. Appropriate control and protection strategies are urgently needed to cut off the main Pb exposure pathway in pregnant women, children, and infants.