Weather and food availability additively affect reproductive output in an expanding raptor population

The joint effects of interacting environmental factors on key demographic parameters can exacerbate or mitigate the separate factors’ effects on population dynamics. Given ongoing changes in climate and land use, assessing interactions between weather and food availability on reproductive performance is crucial to understand and forecast population dynamics. By conducting a feeding experiment in 4 years with different weather conditions, we were able to disentangle the effects of weather, food availability and their interactions on reproductive parameters in an expanding population of the red kite (Milvus milvus), a conservation-relevant raptor known to be supported by anthropogenic feeding. Brood loss occurred mainly during the incubation phase, and was associated with rainfall and low food availability. In contrast, brood loss during the nestling phase occurred mostly due to low temperatures. Survival of last-hatched nestlings and nestling development was enhanced by food supplementation and reduced by adverse weather conditions. However, we found no support for interactive effects of weather and food availability, suggesting that these factors affect reproduction of red kites additively. The results not only suggest that food-weather interactions are prevented by parental life-history trade-offs, but that food availability and weather conditions are crucial separate determinants of reproductive output, and thus population productivity. Overall, our results suggest that the observed increase in spring temperatures and enhanced anthropogenic food resources have contributed to the elevational expansion and the growth of the study population during the last decades.

Uncovering the Sex-Specific Endocrine Responses to Reproduction and Parental Care

Hormones mediate physiological and behavioral changes in adults as they transition into reproduction. In this study, we characterize the circulating levels of five key hormones involved in reproduction in rock doves (Columba livia): corticosterone, progesterone, estradiol, testosterone, and prolactin using univariate and multivariate approaches. We show similar patterns as previous studies in the overall patterns in circulating levels of these hormones, i.e., testosterone (males) and estradiol (females) high during nest-building or egg-laying, prolactin increasing at mid-incubation and peaking at hatching (both sexes), and elevated corticosterone levels in later incubation and early nestling development. In our investigation of hormone co-variation, we find a strong correlation between prolactin and corticosterone across sampling stages and similarities in earlier (early to mid-incubation) compared to later (late incubation to nestling d9) sampling stages in males and females. Finally, we utilized experimental manipulations to simulate nest loss or altered caregiving lengths to test whether external cues, internal timing, or a combination of these factors contributed most to hormone variation. Following nest loss, we found that both males and females responded to the external cue. Males generally responded quickly following nest loss by increasing circulating testosterone, but this response was muted when nest loss occurred early in reproduction. Similar treatment type, e.g., removal of eggs, clustered similarly in hormone space. These results suggest internal drivers limited male response early in reproduction to nest loss. In contrast, circulating levels of these hormones in females either did not change or decreased following nest manipulation suggesting responsiveness to external drivers, but unlike males, this result suggests that reproductive processes were decreasing.

The Influence of Different Light Wavelengths of Anthropogenic Light at Night on Nestling Development and the Timing of Post-fledge Movements in a Migratory Songbird

Many different aspects of an animal’s lifecycle such as its behavior, patterns of hormone activity, and internal clock time, can be affected by anthropogenic light at night (ALAN). Exposing an organism to ALAN during its early life could also have an impact on its development. Since photoperiod can trigger or schedule the migration timing of long-distance migratory birds, there is great potential for anthropogenic light to interact with photoperiod to affect timing. However, very little has been investigated regarding the impacts of ALAN on post-hatching development and migration timing. We investigated the impact of ALAN during nestling development in a long-distance migratory songbird to determine the potential impact on the timing of post-breeding movements in the wild. We experimentally manipulated the light by using programmable lighting, in the nest boxes of free-living nestlings of purple martin (Progne subis) in Manitoba, Canada. We exposed two groups of developing nestlings, from hatch to fledge date, to green or white LED lights (5 lux) during the night. We also included a control group that experienced natural, ambient light at night. We found that some adults abandoned their nests shortly after starting the experiment (4 of 15 nests in the white light treatment). For the nests that remained active, nestlings exposed to the white light treatment had higher weights (at day 20 or 22), later fledge dates (1.54 ± 0.37, 95% CI 0.80–2.28), and later colony departure date (2.84 ± 1.00, 95% CI 0.88–4.81), than young of the control group. Moreover, nestlings of both white and green light groups had longer nesting duration than nestlings of the control group. This study demonstrates the impact of ALAN on the development of post-breeding movement timing in nestlings of wild migratory birds. However, our results also indicate that green light may have less of an impact as compared to white light.

Experimental Evaluation of δ2H, δ13C and δ15N Variability in Blood and Feathers of Wild and Captive Birds: Implications for Interspecific Food Web Studies

Stable-hydrogen (δ2H), nitrogen (δ15N), and carbon (δ13C) isotopes are used to decipher broad movement patterns and trophic relationships among diverse species, and an improved understanding of factors controlling natural variation in tissue-isotope measurements will enhance these applications. To evaluate the rearing environment and family-related effects on the isotopic composition of tissues, we cross-fostered nestling tree swallows (Tachycineta bicolor, Vieillot 1808) and American kestrels (Falco sparverius, Linnaeus 1758) by swapping recently hatched birds (<4 days old) among nest boxes and collecting blood and feathers prior to fledging. To assess developmental effects, we measured δ2H in blood and feathers of captive mallard (Anas platyrhynchos, Linnaeus 1758) ducklings challenged energetically during growth. Stable isotope composition was not strongly related to nest box type or natal nest (i.e., family of origin) effects in swallows and kestrels; tissue-isotope composition was related to rearing environment, indicative of differences in nest and parental quality or parental provisioning tactics. Blood and feather δ2H values in swallows were positively related to antecedent maximum ambient temperature, and unrelated to elevated energy expenditure in mallards. The average differences between δ2H in blood and feathers were similar for nestling swallows (27‰, 32‰; two sites) and mallards (26‰, 30‰; two age groups), and lower than in nestling kestrels (50‰). Strong species-specific patterns in blood-feather differences were not observed for δ15N and δ13C in swallows or kestrels; divergent δ2H results may be related to differences in nest ambient conditions, diet composition, or physiological processes affecting hydrogen assimilation during growth and feather synthesis. In swallows, tissue-isotope values reflected parental prey selection from spatially distinct food webs during nestling development with little effect(s) of family of origin, egg composition, or early growth.

Pulses of anthropogenic food availability appear to benefit parents, but compromise nestling growth in urban red-winged starlings

The provision of anthropogenic food undoubtedly influences urban bird fitness. However, the nature of the impact is unclear, with both benefits and costs of urban diets documented. Moreover, the influence of short-term fluctuations in food availability, linked to urban weekday/weekend cycles of human presence, is largely unknown. We explored whether breeding red-winged starlings Onychognathus morio in Cape Town, South Africa, altered foraging and provisioning behaviour between days with high human presence (HHP) and days with low human presence (LHP)—i.e. weekdays versus weekends and vacation days. We investigated the relationship between starling diet, adult body mass and nestling development. Breeding adults consumed and provisioned the same quantity of food, but a significantly greater proportion of anthropogenic food on HHP compared to LHP days. Adults apparently benefited from the anthropogenic diet, experiencing significantly greater mass gain on HHP days. However, nestlings experienced a cost, with the number of HHP days during the nestling period associated negatively with nestling size. Adults may, therefore, benefit from the high calorie content of anthropogenic food, while nestlings may be negatively affected by nutrient limitation. The quantity of food available in urban environments may, therefore, benefit adult survival, while its quality imposes a cost to nestling growth.

Experimental manipulation of photoperiod influences migration timing in a wild, long-distance migratory songbird

Previous laboratory studies have demonstrated the role of photoperiod in cueing the migration timing of small land birds; however, how migration timing of young birds in wild environments develops in relation to these cues have rarely been investigated. Such investigations can make important contributions to our developing understanding of the phenotypic plasticity of migration timing to new conditions with climate change, where changes in the timing of nesting may expose juvenile birds to different photoperiods. We investigated the impact of manipulating photoperiod during nestling development in a long-distance migratory songbird on the timing of post-breeding movements in the wild. Using programmable lighting installed in the nest-boxes of purple martins ( Progne subis ), we exposed developing nestlings, from hatch to fledge date, to an extended photoperiod that matched the day length of the summer solstice in Manitoba, Canada. We found that birds with a simulated, earlier photoperiod had a longer nesting period and later fledge and autumn departure dates than control group birds. This study demonstrates the phenotypic plasticity of first-year birds to the ontogenetic effect of their hatch date in the formation of the timing of their first post-breeding movements. Further, we discuss how these results have implications for the potential use of assisted evolution approaches to alter migration timing to match new conditions with climate change.

Timing and intensity of weather events shape nestling development strategies in three alpine breeding songbirds

Across taxa, offspring size traits are linked to survival, and life-time fitness. Inclement weather can be a major constraint on offspring growth and parental care. Despite the adaptive benefits of larger offspring, we have a limited understanding of the effects of severe environmental conditions across developmental stages and how coping strategies differ among species. We assessed the influence of inclement weather on offspring size and mass traits within populations of three alpine breeding songbirds in British Columbia: (1) horned lark (Eremophila alpestris) (2) dark-eyed junco (Junco hyemalis), and (3) savannah sparrow (Passerculus sandwichensis). Specifically, we investigated at which stages during early-life development offspring are most vulnerable to inclement weather and whether thresholds exist in the developmental response to severe weather events. Across species, we identified two critical periods that best predicted offspring size: (1) clutch initiation, and (2) the nestling stage. Colder temperatures experienced by the female during clutch initiation were associated with larger, heavier offspring in horned larks but smaller offspring for savannah sparrows, indicating the potential for maternal effects, albeit acting through different mechanisms. Additionally, horned lark offspring were resilient to colder average temperatures during the nestling stage but were vulnerable to extreme cold events and multi-day storms. In contrast, dark-eyed junco nestlings were robust to storms, but smaller size and mass traits were associated with lower daily maximum temperatures (i.e., more mild temperature challenges). We suggest species differences may be linked to life-history traits, such as: (1) the thermoregulatory benefits of larger body mass in horned larks, (2) the benefits of greater nest cover to buffer dark-eyed junco against precipitation events, and (3) delayed clutch initiation for savannah sparrows to limit exposure to cold storms. We provide evidence for stage-specific impacts of inclement weather on offspring development with implications for reproductive success. These results advance our understanding of early-life resilience to stochastic environments, as we may be able to predict differences in the vulnerability of alpine species to increasingly variable and severe weather conditions.

Development of nestling blue tits (Cyanistes caeruleus) is affected by experimental addition of aromatic plants

Some passerines incorporate aromatic plants in their nest cups, and several hypotheses have been formulated to explain this behaviour. One of those, the Drug Hypothesis, states that aromatic plants present in nests have positive effects on nestlings’ development through increased immune function. In this study, we aimed to examine if experimental addition of aromatic plants had positive effects on reproductive performance (the number of fledglings produced and nestling survival rate) and nestling development (weight and tarsus length). In addition, we study whether those potential effects were more noticeable in different brood sizes–small and large–through an observational approach. We expect that large broods, due to increased resource competition between nestlings, will benefit more from aromatic plant incorporation, as compared to small broods. Nestlings were significantly heavier in 2015 as compared to 2016 and 2017 and in small broods. No effect of treatment was observed in nestling weight. Although there was no overall effect of treatment on nestling tarsus length, nestlings from aromatic nests had significantly longer tarsi as compared to nestlings from control nests, in large broods.