Effects of Mating on Gene Expression in Female Insects: Unifying the Field

There is intense interest in controlling insect reproductive output. In many insect species, reproductive output is profoundly influenced by mating, including the receipt of sperm and seminal fluid molecules, through physiological and behavior changes. To understand these changes, many researchers have investigated post-mating gene expression regulation. In this review, we synthesize information from studies both across and within different species about the impact of mating, or components of mating, on female gene expression patterns. We found that genes related to the roles of metabolism, immune-response, and chemosensation are regulated by mating across many different insect species. We highlight the few studies that have taken the important next step of examining the functional consequences of gene expression regulation which is crucial in order to understand the mechanisms underlying the mating-regulated control of female lifespan and reproduction and to make use of such knowledge to propagate or control insect populations. The potential of cross-study comparisons is diminished by different studies using different methods. Thus, we also include a consideration of how future studies could be designed to facilitate cross-study comparisons and a call for collaboration across researchers studying different insect species and different aspects of insect biology.

Sublethal exposure to an emulsion based on Pogostemon cablin (Lamiaceae) essential oil impairs digestibility, feeding, fecundity, and mobility of the coffee berry borer, Hypothenemus hampei

The global search for eco-friendly and human-safe pesticides has intensified, and research on essential oils (EOs) has expanded due to their remarkable insecticidal activities and apparent human-safe. Despite this, most of the literature focuses on short-term and simplified efforts to understand lethal effects, with only a few comprehensive studies addressing sublethal exposures. To fill this shortcoming, we explore the lethal and sublethal effects of Pogostemon cablin (Lamiaceae) EO and an EO-based emulsion (18%) using the coffee berry borer Hypothenemus hampei Ferrari (Coleoptera: Curculionidae: Scolytinae) as a model. First, we determine the toxicity of EO and EO-based emulsion using dose-mortality curves and lethal times. Second, we subjected adult females of H. hampei to sublethal doses to assess whether they affected their behavior, reproductive output, and histological features. Our findings reveal that patchoulol (43.05%), α-Guaiene (16.06%), and α-Bulnesene (13.69%) were the main components of the EO. Furthermore, the EO and its emulsion had similar toxicity, with dose-mortality curves and lethal times overlapping 95% confidence intervals. We also observed that sublethal exposure of females of H. hampei reduces reproduction and feeding, increases walking activity, and causes histopathological changes in the midgut. This study advances the knowledge of sublethal effects of an eco-friendly substance on insects.

Delayed timing of breeding attempts, but not time lost to nest construction, reduces the annual reproductive output of the Eastern Phoebe (Sayornis phoebe)

For many birds, nest construction is a costly aspect of parental care, trading finite energetic resources between parental care and self-maintenance. For multi-brooded organisms with short breeding seasons, such as migratory passerines, repeated nest construction could be especially costly if the activity delays the onset of breeding attempts. Earlier studies on passerines that reuse nests between breeding seasons suggested that time lost to initial nest construction reduces seasonal reproductive output. However, costs associated with building new nests between breeding attempts, within the same breeding season, have largely been ignored. Here, we experimentally removed first nests, after fledging or failing, of Eastern Phoebes ( Sayornis phoebe), to evaluate how the annual onset of breeding and nest construction between breeding attempts affected parental investment into second attempts. We found that first egg laying date negatively predicted the probability of second breeding attempts, but experimental treatment (first nest removal vs. control) did not. Neither first egg laying date nor treatment statistically influenced any of the reproductive traits in second breeding attempts (clutch size, nestling body condition, and nestling growth rate). We conclude that in this species, second breeding attempts are limited by the initial onset of seasonal reproduction, and not by time lost to nest construction between breeding attempts.

Linking individual and population patterns of rocky-shore mussels

Individual traits and population parameters can be used as proxies of processes taking place within a range of scales, thus improving the way we can evaluate species response to environmental variability. In intertidal rocky shores, patterns at the within-site scale, i.e., between centimeters to hundreds of meters, are important for understanding the population response into these highly variable environments. Here, we studied a rocky-shore mussel population at the within-site spatial scale (1) to test how intertidal height and orientation of the shore affect individual traits and population parameters, (2) to infer the link between individual and population level features, and (3) to explore the upscaling mechanisms driving population structure and processes. We analyzed the patterns of six population parameters: density, biomass, crowding, median individual size, recruitment and mortality rate, and four individual traits: growth rate, spawning phenology, size and condition index. Crowding was defined as the degree of overlapping of individuals within a given area, for which we created a “crowding index”. Mussels were studied along the intertidal height gradient in two rocky shores with contrasted orientation at one site over a full year. Our results showed a significant effect of intertidal height and shore orientation on most of individual traits and population parameters studied. In contrast, biomass contained in a full covered surface did not vary in space nor in time. This pattern likely results from relatively constant crowding and a trade-off between median individuals’ size and density. We hypothesize that growth, mortality and recruitment rates may all play roles in the stability of the crowding structure of mussel aggregations. Variation in spawning phenology between the two shores in the study site was also observed, suggesting different temporal dynamics of microclimate conditions. Interestingly, despite the different population size distribution between the two shores, our estimates indicate similar potential reproductive output. We hypothesize that the structure of the patches would tend to maintain or carry a maximum of biomass due to trade-offs between density and size while maintaining and maximizing the reproductive output. The patterns of spatial variability of individual traits and population parameters in our study site suggest that heterogeneous within-site conditions influence variation in individual performance and population processes. These results provide insights about the relationship between individual traits and how these relationships make patterns at the population level emerge. They provide baseline information necessary to improve models of metapopulation with spatially explicit processes.

Reproductive Success of an Endangered Plant after Invasive Bees Supplant Native Pollinator Services

Arctomecon humilis (dwarf bear poppy) is an endangered gypsophile restricted to the St. George area in southern Utah, USA. Extirpation of the rare oligolectic bee Perdita meconis and decline of other native pollinators within the last 20 years has been largely attributed to the advent of invasive Africanized honeybees (AHBs), which have become the predominant pollinator for A. humilis. A three-year study was carried out to determine if reliance on AHBs had a negative effect on reproductive output. In the first year, pollen addition experiments were carried out in a small, isolated population to determine if pollinator scarcity or genetic factors affected reproductive output. In the subsequent two years, reproductive output studies were conducted across four populations. In all years, fruit set across study sites was exceptionally high. There was no consistent effect from flower number or interplant distance, which have historically been shown to have a significant impact on reproductive output prior to AHB arrival. The consequences and sustainability of this relatively young relationship between A. humilis and the AHB remain unclear. However, high reproductive success shows that the AHB is currently capable of adequately fulfilling pollinator services for A. humilis.

Bigger Is Better, Sometimes: The Interaction between Body Size and Carcass Size Determines Fitness, Reproductive Strategies, and Senescence in Two Species of Burying Beetles

The cost of reproduction hypothesis suggests that allocation to current reproduction constrains future reproduction. How organisms accrue reproductive costs and allocate energy across their lifetime may differ among species adapted to different resource types. We test this by comparing lifetime reproductive output, patterns of reproductive allocation, and senescence between two species of burying beetles, Nicrophorus marginatus and N. guttula, that differ in body size, across a range of carcass sizes. These two species of burying beetles maximized lifetime reproductive output on somewhat different–sized resources. The larger N. marginatus did better on large and medium carcasses while the smaller N. guttula did best on small and medium carcasses. For both species, reproduction is costly and reproduction on larger carcasses reduced lifespan more than reproduction on smaller carcasses. Carcass size also affected lifetime reproductive strategies. Each species’ parental investment patterns were consistent with terminal investment on carcasses on which they performed best (optimal carcass sizes). However, they exhibited reproductive restraint on carcass sizes on which they did not perform as well. Reproductive senescence occurred largely in response to carcass size. For both species, reproduction on larger carcasses resulted in more rapid senescence. These data suggest that whether organisms exhibit terminal investment or reproductive restraint may depend on type and amount of resources for reproduction.

Ecology of the Possum (Trichosurus Vulpecula Kerr) in the Karori Water Supply Reserve, Wellington, Sounds Ecological District

<p>The abundance and condition of possums in various areas of the Karori Reserve, Wellington - Sounds Ecological District was assessed. Possums were most abundant in the upper part of the reserve in an area dominated by bishop and radiata pines with an undergrowth of native species and in an area dominated by gorse and flax shrublands. In the lower part of the reserve, possums were less abundant and there a number of characteristics were observed: possums had a higher body weight for age, their reproductive output was higher and the population was marked by a higher proportion of females and juveniles. This was interpreted as a "compensatory response" characteristic of possum populations maintained at low densities. Possum stomachs were sampled in spring and their contents analysed in order to investigate the importance of Fuchsia excorticata and other plant species to possum diet. In the lower part of the reserve the leaves of Fuchsia excorticata and the flowers of Cytisus scoparius made up more than 50 percent of the possums' forage. In the upper part of the reserve exotic species such as the flowers of Cytisus scoparius and Ulex europeans as well as the leaves of native species such as Aristotelia serrata, Pseudopanax arboreus and Coprosma robusta made up the bulk of possum forage. Exotic plants accounted for a significant proportion of the spring possum diet in the reserve. Whereas most leaves originated from native plants, the exotic species contributed mainly carbohydrate-rich flowers and pollen. The canopy condition of two possum-susceptible tree species Fuchsia excorticata and Pseudopanax arboreus was assessed early in autumn over the years 1993, 1995 and 1996. Over these years, 47 percent of marked fuchsia trees were completely defoliated and most trees are unlikely to recover even if possums are eradicated from the reserve. All defoliated fuchsia trees were located in the upper part of the reserve. On the other hand, 37 percent of marked fuchsia trees exhibited only a little possum damage. Most of these trees were located in the lower part of the reserve where possum density was lower. Increasing possum densities could therefore be correlated with an increasing level of possum damage to fuchsia canopies. Even though Pseudopanax arboreus leaves and petioles were encountered in 10 percent of possum stomachs, the canopies leaves and petioles were encountered in 10 percent of possum stomachs, the canopies of assessed trees did not seem to be greatly affected. It appeared that due to the high abundance of this plant species in the reserve the effects of possum browse were spread over the whole reserve to levels which could be tolerated by an individual Pseudopanax arboreus tree.</p>

Three rivers and a fish: body shape variation in adult inanga (Galaxias maculatus) and implications for reproductive output

<p>Inanga (Galaxias maculatus) are the major component of New Zealand’s whitebait fisheries. Monitoring of freshwater-based adult populations in both North and South Island river systems suggests a general decline in numbers of fish, and specific streams and watersheds are of particular concern for some regional councils. Given this context, improved information about the environmental conditions that influence inanga’s reproductive output may help to inform appropriate management actions and improve the long term viability of these populations. The breeding biology of adult inanga has been the focus of considerable research effort, with restoration of spawning grounds further extending our knowledge of spatio-temporal patterns of spawning. However, the behaviour patterns and fates of adult fish outside of the spawning locations and seasons are poorly known, as are the factors that may influence the survival and development of pre-spawning stages of inanga. Variation in body shape and size influences multiple performance and fitness attributes, and has major implications for reproduction. My thesis aims to quantify relationships between morphology and reproductive output for adult inanga, and to investigate environmental factors that may influence morphological development. I collected inanga at two discrete stages of adult development: (i) pre-spawning stage adults at upstream habitats and (ii) spawning-stage adults collected at known breeding grounds during the spawning season. I photographed inanga in the field using a purpose-built aquarium and measured a set of morphological characteristics related to reproductive output and swimming ability. Specifically, I measured standard length, head depth, body depth and caudal peduncle depth. A subsample of adult inanga collected during the spawning season were returned to the laboratory and euthanized to measure aspects of their reproductive biology. Specifically, I measured reproductive output using gonad weight, I estimated maturity using the gonadosomatic index (GSI: weight of the gonad relative to total body weight), and I estimated energetic reserves using the hepatosomatic index (HSI: weight of the liver relative to total body weight). Pre-spawning stage inanga in the Waiwhetu Stream displayed deeper bodies than fish in either the Hutt or Wainuiomata Rivers, potentially due to higher concentrations of food. Inanga in the Wainuiomata River displayed slightly larger sizes prior to the spawning season and substantially larger bodies during the spawning season. Although the Waiwhetu Stream may have had a greater supply of food, high densities of fish in smaller stream systems could constrain adult growth. Spawning-stage adults collected from the Waiwhetu Stream had greater reproductive output than fish collected from the Hutt River, with a peak in spawning activity during May. Fish collected from spawning grounds in the Hutt River had lower reproductive output and two peaks in spawning activity during March and May. Inanga in the Hutt River also displayed greater within-month variation in the maturity of fish. My results suggest that spawning grounds in larger, more complex river systems (e.g., the Hutt River) may be supplied by inanga from a diverse range of main stem habitats and smaller tributaries further inland. Smaller systems (e.g., the Waiwhetu Stream) may be comprised of a more homogeneous population of inanga, and reproductive output of the system as a whole may be greater, but concentrated over a shorter time period. I hypothesise that the reproductive output from larger river systems may be more resilient to disturbance events (e.g., stock trampling of spawning grounds) because these systems (by virtue of their greater diversity of habitats and phenotypes of fish) may enable multiple opportunities for spawning. I suggest that larger rivers, such as the Hutt River, may be of disproportionate importance (independent of their total reproductive output) for the replenishment of inanga stocks.</p>

Three rivers and a fish: body shape variation in adult inanga (Galaxias maculatus) and implications for reproductive output

<p>Inanga (Galaxias maculatus) are the major component of New Zealand’s whitebait fisheries. Monitoring of freshwater-based adult populations in both North and South Island river systems suggests a general decline in numbers of fish, and specific streams and watersheds are of particular concern for some regional councils. Given this context, improved information about the environmental conditions that influence inanga’s reproductive output may help to inform appropriate management actions and improve the long term viability of these populations. The breeding biology of adult inanga has been the focus of considerable research effort, with restoration of spawning grounds further extending our knowledge of spatio-temporal patterns of spawning. However, the behaviour patterns and fates of adult fish outside of the spawning locations and seasons are poorly known, as are the factors that may influence the survival and development of pre-spawning stages of inanga. Variation in body shape and size influences multiple performance and fitness attributes, and has major implications for reproduction. My thesis aims to quantify relationships between morphology and reproductive output for adult inanga, and to investigate environmental factors that may influence morphological development. I collected inanga at two discrete stages of adult development: (i) pre-spawning stage adults at upstream habitats and (ii) spawning-stage adults collected at known breeding grounds during the spawning season. I photographed inanga in the field using a purpose-built aquarium and measured a set of morphological characteristics related to reproductive output and swimming ability. Specifically, I measured standard length, head depth, body depth and caudal peduncle depth. A subsample of adult inanga collected during the spawning season were returned to the laboratory and euthanized to measure aspects of their reproductive biology. Specifically, I measured reproductive output using gonad weight, I estimated maturity using the gonadosomatic index (GSI: weight of the gonad relative to total body weight), and I estimated energetic reserves using the hepatosomatic index (HSI: weight of the liver relative to total body weight). Pre-spawning stage inanga in the Waiwhetu Stream displayed deeper bodies than fish in either the Hutt or Wainuiomata Rivers, potentially due to higher concentrations of food. Inanga in the Wainuiomata River displayed slightly larger sizes prior to the spawning season and substantially larger bodies during the spawning season. Although the Waiwhetu Stream may have had a greater supply of food, high densities of fish in smaller stream systems could constrain adult growth. Spawning-stage adults collected from the Waiwhetu Stream had greater reproductive output than fish collected from the Hutt River, with a peak in spawning activity during May. Fish collected from spawning grounds in the Hutt River had lower reproductive output and two peaks in spawning activity during March and May. Inanga in the Hutt River also displayed greater within-month variation in the maturity of fish. My results suggest that spawning grounds in larger, more complex river systems (e.g., the Hutt River) may be supplied by inanga from a diverse range of main stem habitats and smaller tributaries further inland. Smaller systems (e.g., the Waiwhetu Stream) may be comprised of a more homogeneous population of inanga, and reproductive output of the system as a whole may be greater, but concentrated over a shorter time period. I hypothesise that the reproductive output from larger river systems may be more resilient to disturbance events (e.g., stock trampling of spawning grounds) because these systems (by virtue of their greater diversity of habitats and phenotypes of fish) may enable multiple opportunities for spawning. I suggest that larger rivers, such as the Hutt River, may be of disproportionate importance (independent of their total reproductive output) for the replenishment of inanga stocks.</p>