Unraveling the hydrology and sediment balance of an ungauged lake in the Sudano-Sahelian region of West Africa using remote sensing

The presence of ephemeral ponds and perennial lakes in the Sudano-Sahelian region of West Africa is strongly variable in space and time. Yet, they have important ecological functions and societies are reliant on their surface waters for their lives and livelihoods. It is essential to monitor and understand the dynamics of these lakes to assess past, present, and future water resource changes. In this paper, we present an innovative approach to unravel the sediment and water balance of Lac Wégnia, a small ungauged lake in Mali near the capital of Bamako. The approach uses optical remote sensing data to identify the shoreline positions over a period of 22 years (2000–2021) and then attributes water surface heights (WSHs) to each observation using the lake bathymetry. The method represents a significant advancement over previous attempts to remotely monitor lakes in the West African drylands, since it considers not only changes in water depth to explain recent declining trends in lake areas, but also changes in the storage capacity. We recognize silting at the tributaries to the lake, but overall, erosion processes are dominant and threaten the persistence of the lake because of a continuous decrease of the floor level at the outflow. This explains the decreasing trend in WSH even for the wet-season, in spite of positive rainfall patterns.

Embryonic developmental arrest in the annual killifish Austrolebias charrua: A proteomic approach to diapause III

Diapause is a reversible developmental arrest faced by many organisms in harsh environments. Annual killifish present this mechanism in three possible stages of development. Killifish are freshwater teleosts from Africa and America that live in ephemeral ponds, which dry up in the dry season. The juvenile and adult populations die, and the embryos remain buried in the bottom mud until the next rainy season. Thus, species survival is entirely embryo-dependent, and they are perhaps the most remarkable extremophile organisms among vertebrates. The aim of the present study was to gather information about embryonic diapauses with the use of a “shotgun” proteomics approach in diapause III and prehatching Austrolebias charrua embryos. Our results provide insight into the molecular mechanisms of diapause III. Data are available via ProteomeXchange with identifier PXD025196. We detected a diapause-dependent change in a large group of proteins involved in different functions, such as metabolic pathways and stress tolerance, as well as proteins related to DNA repair and epigenetic modifications. Furthermore, we observed a diapause-associated switch in cytoskeletal proteins. This first glance into global protein expression differences between prehatching and diapause III could provide clues regarding the induction/maintenance of this developmental arrest in A. charrua embryos. There appears to be no single mechanism underlying diapause and the present data expand our knowledge of the molecular basis of diapause regulation. This information will be useful for future comparative approaches among different diapauses in annual killifish and/or other organisms that experience developmental arrest.

Metabolomics analysis of annual killifish (Austrofundulus limnaeus) embryos during aerial dehydration stress

The annual killifish, Austrofundulus limnaeus, survives in ephemeral ponds in the coastal deserts of Venezuela. Persistence through the dry season is dependent on drought-resistant eggs embedded in the pond sediments during the rainy season. The ability of these embryos to enter drastic metabolic dormancy (diapause) during normal development enables A. limnaeus to survive conditions lethal to most other aquatic vertebrates; critical to the survival of the species is the ability of embryos to survive months and perhaps years without access to liquid water. Little is known about the molecular mechanisms that aid in survival of the dry season. This study aims to gain insight into the mechanisms facilitating survival of dehydration stress due to aerial exposure by examining metabolite profiles of dormant and developing embryos. There is strong evidence for unique metabolic profiles based on developmental stage and length of aerial exposure. Actively developing embryos exhibit more robust changes; however, dormant embryos respond in an active manner and significantly alter their metabolic profile. A number of metabolites accumulate in aerial-exposed embryos that may play an important role in survival, including the identification of known antioxidants and neuroprotectants. In addition, a number of unique metabolites not yet discussed in the dehydration literature are identified, such as lanthionine and 2-hydroxyglutarate. Despite high oxygen availability, embryos accumulate the anaerobic end product lactate. This paper offers an overview of the metabolic changes occurring that may support embryonic survival during dehydration stress due to aerial incubation, which can be functionally tested using genetic and pharmacological approaches.

GABA metabolism is crucial for long-term survival of anoxia in annual killifish embryos

ABSTRACTIn most vertebrates, a lack of oxygen quickly leads to irreparable damages to vital organs, such as the brain and heart. However, there are some vertebrates that have evolved mechanisms to survive periods of no oxygen (anoxia). The annual killifish (Austrofundulus limnaeus) survives in ephemeral ponds in the coastal deserts of Venezuela and their embryos have the remarkable ability to tolerate anoxia for months. When exposed to anoxia, embryos of A. limnaeus respond by producing significant amounts of γ-aminobutyric acid (GABA). This study aims to understand the role of GABA in supporting the metabolic response to anoxia. To explore this, we investigated four developmentally distinct stages of A. limnaeus embryos that vary in their anoxia tolerance. We measured GABA and lactate concentrations across development in response to anoxia and aerobic recovery. We then inhibited enzymes responsible for the production and degradation of GABA and observed GABA and lactate concentrations, as well as embryo mortality. Here, we show for the first time that GABA metabolism affects anoxia tolerance in A. limnaeus embryos. Inhibition of enzymes responsible for GABA production (glutamate decarboxylase) and degradation (GABA-transaminase and succinic acid semialdehyde dehydrogenase) led to increased mortality, supporting a role for GABA as an intermediate product and not a metabolic end-product. We propose multiple roles for GABA during anoxia and aerobic recovery in A. limnaeus embryos, serving as a neurotransmitter, an energy source, and an anti-oxidant.

Lithostratigraphy, facies, mineralogy and diagenesis of the retrograding, syntectonic Neogene Barzaman Formation (Al-Khod, Sultanate of Oman)

<p>The Barzaman Formation is 150-200 m thick and subdivided into five lithostratigraphic/facies intervals recording syndepositional thrusting and changes from shallow marine to terrestrial environments and from arid/semiarid to more humid conditions.</p><p>(1) The basal lower conglomerate and sandstone unit is >36 m thick, marked by beige and gray/greenish colors, thick-bedded pebbly, calciclastic litharenites which may display parallel lamination and thick-bedded matrix-supported pebble to cobble conglomerates with subrounded clasts of chert, basalt, gabbro, quartzite and carbonates. Pores may be lined by isopachous, microcrystalline calcite cement. The depositional environment is shallow marine with one coarse-grained fill of a high-energy tidal inlet.</p><p>(2) The light-colored carbonate facies unit is 1-15 m thick, consisting of thick-bedded coral limestone, a very thick limestone coral and algae debrite and some minor beds of conglomerate and sandstone. The corals may be partly silicified by brown-stained silica. This unit was deposited in a warm, shallow marine, nearshore environment with clear water which may indicate an arid climate.</p><p>(3) The varicolored thick sandstone and conglomerate facies unit is 14-28.5 m thick. These clastic deposits are similar to those of unit 1, but more colorful, slightly coarser grained (presence of boulders) and include also thin and medium beds. The sandstones may exhibit cross-bedding. The depositional environment is shallow marine as indicated by coral debris.</p><p>(4) The claystone and conglomerate facies unit is 19 m thick. The clastic sediments are similar to those of unit 1, but pebbly sandstones are comparatively rare, and claystone beds are present, including a 20-cm-thick cellular claystone (palygorskite, vermiculite with some calcite) as well as light gray, medium-bedded claystone beds, consisting mainly of palygorskite with some saponite and/or clinochlore, associated with minute, euhedral dolomite or ankerite crystals. All claystone beds are evaporitic, lacustrine deposits of ephemeral ponds and pools on wadi floors whereas the coarser beds represent wadi conglomerates. Some beds are imbricated slide units. The paleoclimate was hot, semiarid or arid.</p><p>(5) The dolomitic conglomerate facies unit may measure >61 m in thickness. The respective pebble conglomerates consist of clasts that seem to “float” in cement. The cements of the basal >10 m are brown-stained silica and some white dolomite. The silica content gradually decreases upward. The upper part is dominated by white dolomite and some calcite. The dolomite cement may have formed under phreatic conditions (groundwater) during the Late Miocene to Pliocene when the arid/semiarid Miocene climate became more humid.    </p><p>Close to the base of unit 4, the upper part of an east-dipping syndepositional thrust is exposed (Mattern et al., 2018). Faulting approximately coincides with the change from marine to terrestrial conditions. In addition, the syndepostional tectonic activity may explain aspects of slope instability: debrite in unit 2, slide units in unit 4.</p><p> </p><p>References</p><p>Mattern, F., Scharf, A., Al-Amri, S.H.K., 2018. East-west directed Cenozoic compression in the Muscat area (NE Oman): timing and causes. Gulf Seismic Forum, 19-22 March 2018, Muscat, Oman, Book of Abstracts, p. 4-7.</p>

Remarks on Mastigodiaptomus (Calanoida: Diaptomidae) from Mexico using integrative taxonomy, with a key of identification and three new species

Background In Mexico, species of four families of free-living calanoid copepods have been recorded as inhabitants of several freshwater systems. These families are Centropagidae, Temoridae, Pseudodiaptomidae and Diaptomidae. The genera Leptodiaptomus and Mastigodiaptomus are the most speciose diaptomid genera in Mexico, and they inhabit natural and artificial lakes, ephemeral ponds, springs, and caverns. Leptodiaptomus is considered as an endemic Nearctic genus, whereas Mastigodiaptomus is a widely distributed Neotropical genus in the southern USA, Mexico, the Caribbean Islands and Central America. Based on new and recent evidence, Mastigodiaptomus diversity has been underestimated: six species of the genus were known before 2000. In this work three new Mastigodiaptomus species have been described from different regions of Mexico by using integrative taxonomy. We also gave amended diagnosis of M. nesus Bowman (1986) and M. patzcuarensis s. str. (Kiefer, 1938). Methods In this work, the taxonomic status of the species was clarified using modern, integrative method based on the COI gene as a DNA marker, plus micro-structural analysis (based on SEM and ligth microscopy). Results Three new species of Mastigodiaptomus were described based on genetic and morphological analyses: M. alexei sp. n., M. ha sp. n. and M. cihuatlan sp. n. Also amended description of M. nesus, morphological variation of M. patzcuarensis s. str., and a comparison of them with all known sequences within the genus are provided. These new findings show that in Mastigodiaptomus differences in several cuticular microstructures of several appendages (such as the antennules, the fifth legs, or the urosomites of these copepods) agree with the interspecific genetic divergence >3% observed in sequences of the COI gene, and the integration of this information is a powerful tool in species delineation.