Lungworm infection with Otostrongylus circumlitus in a free-ranging harbor seal (Phoca vitulina)

A young wild harbor seal (Phoca vitulina) was found stranded on the North Sea coast at Kok- sijde (Belgium)and was admitted to a rehabilitation center where it died one day later. Full ne- cropsy with histopathology was performed. Gross examination revealed severe diffuse hemorr- hagic and emphysematous pneumonia with the presence of 6-10 cm-large nematodes. The nema- todes were found in the trachea, large bronchi, right heart and pulmonary arteries, and subsequently identified as Otostrongylus circumlitus. Histologic examination demonstrated severe eosinophilic to granulomatous bronchointerstitial pneumonia with intralesional nematodes. It was concluded that the seal died of severe verminous pneumonia. In this case report, the importance of lungworm infection, which is a common and critical disease in young seals, is highlighted.

Infectious Bovine Respiratory Diseases in Adult Cattle: An Extensive Necropsic and Etiological Study

In young cattle, bovine respiratory disease (BRD) is a major cause of death and Mannheimia haemolytica is a frequent pathogen. Knowledge of fatal BRD in adult cattle is more limited. We assessed the importance of infectious BRD as a cause of death in adult cattle and determined the associated pathogens. We analyzed data from 737 adult cattle necropsies at the Pathology Unit for Large Animals at Oniris, Nantes, France over a 6 year period (2013–2019). Each carcass was subjected to a complete necropsy. Lungs showing macroscopic lesions were classified into three categories: infectious primary pulmonary (IPP) lesions, thromboembolic pneumonia (TEP) and others (aspiration pneumonia, verminous pneumonia, and local extension of an extra-pulmonary inflammatory process). Half of the lungs with IPP macroscopic lesions were sampled for histology and submitted for polymerase chain reaction. BRD was the second leading cause of death (15.7%) after digestive diseases (32.2%). A strong predominance of IPP lesions (42.3%) and TEP lesions (39.6%) was also demonstrated. In IPP macroscopic lesions, fibrinous, hemorrhagic and/or hecrotic (FHN) bronchopneumonia accounted for 77.6% of macroscopic lesions. Mannheimia haemolytica was significantly associated with FHN bronchopneumonia macroscopic lesions. This study suggests that Mannheimia haemolytica should be included in the differential diagnosis of BRD in adult cattle.

Case Report: Subclinical Verminous Pneumonia and High Ambient Temperatures Had Severe Impact on the Anesthesia of Semi-domesticated Eurasian Tundra Reindeer (Rangifer tarandus tarandus) With Medetomidine–Ketamine

Semidomesticated Eurasian tundra reindeer (Rangifer tarandus tarandus, n = 21) were scheduled twice for chemical immobilization with medetomidine–ketamine as part of a scientific experiment in June 2014. During the first round of immobilizations, seven animals developed severe respiratory depression (RD). Three individuals died, and 4 recovered. The ambient temperature during the 2 days of immobilization (June 3 and 4) was high (mean 13.9–17.6°C) compared to the normal mean temperature for these 2 days (7–8°C) based on statistical records. During the second round of immobilizations, using the same anesthetic protocol for the remaining animals as in the first round but conducted under cooler conditions (mean 6.6°C for the period June 9–18), no signs of RD were observed. Clinical and pathological investigations indicated that the animals suffered from circulatory changes possibly caused by high ambient temperatures and granulomatous interstitial pneumonia due to Elaphostrongylus rangiferi larvae. These conditions, together with the cardiovascular effects of medetomidine, were likely causes of RD and the fatal outcome. We conclude that chemical immobilization of reindeer with medetomidine–ketamine should be avoided in May–June due to the potential risk when animals partly in winter coats encounter rising ambient temperatures and usually have parasites developing in their airways.

Fatal pulmonary parafilaroidiasis in a free-ranging subantarctic fur seal (Arctocephalus tropicalis) coinfected with two gammaherpesviruses and Sarcocystis sp.

A juvenile subantarctic fur seal (Arctocephalus tropicalis) found dead in Santa Catarina state, southern Brazil, presented with disseminated verminous pneumonia due to Parafilaroides sp. A concomitant infection with two different gammaherpesviruses was identified by PCR in different tissues; one of them possibly a novel species (tentatively named Otariid herpesvirus 7). Sarcocystis sp. DNA was identified molecularly in skeletal muscle samples with intrasarcoplasmic bradyzoites and no apparent tissue response. All analyzed samples (mandibular, laryngeal, tracheal, and mesenteric lymph nodes, and lung) were PCR-negative for Brucella spp. The most likely cause of death was severe pulmonary parafilaroidiasis. The pathogenic role of the gammaherpesviruses in several of the tissues was not evident. This study describes the pathogenicity of Parafilaroides sp. in a subantarctic fur seal, widens the host range of herpesvirus in pinnipeds, and reports the first molecular identification of Sarcocystis sp. in this species.

Toxocara canis Larval Migration Causing Verminous Pneumonia in Fading Puppies From 2 Scottish Terrier Litters

Migration of vertically transmitted Toxocara canis larvae through the liver and lungs is poorly documented as a cause of periparturient mortality in puppies. This case series describes 4 cases of fading puppies in 2 litters from 2 different bitches owned by the same breeder. Of the 4 cases, 4 had verminous pneumonia, 2 had fibrinoid necrosis of pulmonary arterioles, 4 had hepatic necrosis and inflammation, 2 had hepatic thrombophlebitis, and 1 had tracheal occlusion. These lesions were associated with migrating nematode larvae morphologically consistent with T. canis. The identity of the larvae was confirmed by sequencing of a portion of the ITS-2 region of nuclear ribosomal DNA. The tissues involved are consistent with the known migration pathways of this parasite. The dam of the first litter was negative for Toxocara spp. and other intestinal parasites by fecal floatation. This report highlights the need to consider T. canis migration in the differential diagnosis of fading puppies.

VERMINOUS PNEUMONIA IN A CALF FROM A DAIRY FARM UNIT, A CASE STUDY

Following a report of a respiratory problem in a calf’s head with an approximate age of 60 days, a livestock unit has undergone basic examinations. Symptoms such as coughing, epistaxis and fever of 40°C were observed. Antibiotics along with serum were prescribed as treatment but the calf has been wasted after a week. With an autopsy and the lung examination, a huge amount of white and thick mucosal secretions as well as a number of parasite larvae have also been isolated within the bronchi. The morphological characteristics of these larvae have been confirmed by Dictyocaulus viviparus parasite infection. Basically, the role of parasitic agents in the development of pneumonia in calves is very significant. Due to the lesser consideration of the role of parasitic agents in the calf’s pneumonia and in addition to the high cost of treatment, this, ultimately leads to the death of calves. Therefore, in dealing with such cases, Para clinical tests such as stool test is also suggested