Surface and Bulk Oxygen Kinetics of BaCo0.4Fe0.4Zr0.2−XYXO3−δ Triple Conducting Electrode Materials

Triple ionic-electronic conductors have received much attention as electrode materials. In this work, the bulk characteristics of oxygen diffusion and surface exchange were determined for the triple-conducting BaCo0.4Fe0.4Zr0.2−XYXO3−δ suite of samples. Y substitution increased the overall size of the lattice due to dopant ionic radius and the concomitant formation of oxygen vacancies. Oxygen permeation measurements exhibited a three-fold decrease in oxygen permeation flux with increasing Y substitution. The DC total conductivity exhibited a similar decrease with increasing Y substitution. These relatively small changes are coupled with an order of magnitude increase in surface exchange rates from Zr-doped to Y-doped samples as observed by conductivity relaxation experiments. The results indicate that Y-doping inhibits bulk O2− conduction while improving the oxygen reduction surface reaction, suggesting better electrode performance for proton-conducting systems with greater Y substitution.

Spleen Emptying Does Not Correlate With Faster Oxygen Kinetics During a Step-Transition Supine Cycling

This manuscript quantified spleen volume changes and examined the relationship between those changes and V̇O2 kinetics during supine cycling. Ten volunteers (age=22±3), completed three step-transitions from 20 W to their power output at 90% gas exchange threshold. Ultrasonic measurements of the spleen were performed each minute. The largest spleen volume reduction was 105 mL (p=.001). No associations existed between: i) spleen volumes at rest ii) spleen volume changes (%) and τV̇O2p. Larger resting spleen volume and greater emptying do not correlate with a faster τV̇O2p. Novelty: • Greater splenic contractions do not augment τV̇O2p, irrespective of spleen emptying and subsequent erythrocyte release.

Effect of PEMF on Muscle Oxygenation during Cycling: A Single-Blind Controlled Pilot Study

Pulsed electromagnetic fields (PEMFs) are used as non-invasive tools to enhance microcirculation and tissue oxygenation, with a modulatory influence on the microvasculature. This study aimed to measure the acute effect of PEMF on muscle oxygenation and its influence on pulmonary oxygen kinetics during exercise. Eighteen male cyclists performed, on different days, a constant-load exercise in both active (ON) and inactive (OFF) PEMF stimulations while deoxyhemoglobin and pulmonary oxygen kinetics, total oxygenation index, and blood lactate were collected. PEMF enhanced muscle oxygenation, with higher values of deoxyhemoglobin both at the primary component and at the steady-state level. Moreover, PEMF accelerated deoxyhemoglobin on-transition kinetic, with a shorter time delay, time constant, and mean response time than the OFF condition. Lactate concentration was higher during stimulation. No differences were found for total oxygenation index and pulmonary oxygen kinetics. Local application of a precise PEMF stimulation can increase the rate of the muscle O2 extraction and utilization. These changes were not accompanied by faster oxygen kinetics, reduced oxygen slow component, or reduced blood lactate level. It seems that oxygen consumption is more influenced by exercise involving large muscle mass like cycling, whereas PEMF might only act at the local level.

An evaluation of factors affecting show jumping warm-up on subsequent show jumping performance in 1.30 m class

Show jumping causes physical and physiological stress on horses’ musculoskeletal structures, which can lead to decreased performance and injury. Appropriate warm-ups can enhance performance, decrease injury risk, as well as increase oxygen kinetics for better efficiency. Despite this, little is known for how warm-up routines affect show jumping performance. Forty-five warm-up routines of show jumpers preparing to enter the show ring were recorded and analysed. Kruskal Wallis analyses with post-hoc Mann Whitney U tests identified if the number of classes combinations completed, types of jumps attempted, warm-up duration, and time spent in each gait during the warm-up varied with rider and horse sex and age, and faults. Spearman correlations assessed if relationships occurred between warm-up duration and content, and the number of faults in the show ring, and horse age. Warm-up ranged from 3:51 to 62:46 min (median 15:09 min) and included at least two jumps (range 2-15). Walk was the most common gait, while upright fences were jumped the most. Knocking down or refusing a fence when warming up did not affect performance. Male riders jumped uprights twice as much as female riders (P<0.03) but this did not impact their performances. Jumping a class prior to the 1.30 affected warm-up, competitors spent longer on the flat before jumping in they had competed earlier in the day (P<0.05) and had fewer jump attempts if they had competed in the class just prior to the 1.30 m (P<0.007). Even though no significant differences were detected, combinations which accumulated >8 faults spent less time warming up. These results suggest warm-up tactics, riders and horses’ age and sex did not influence significantly fault accumulation in the show ring, however warm up routines were influenced by rider decision making and horse age.

Cytochrome c Oxidase Subunit 4 Isoform Exchange Results in Modulation of Oxygen Affinity

Cytochrome c oxidase (COX) is regulated through tissue-, development- or environment-controlled expression of subunit isoforms. The COX4 subunit is thought to optimize respiratory chain function according to oxygen-controlled expression of its isoforms COX4i1 and COX4i2. However, biochemical mechanisms of regulation by the two variants are only partly understood. We created an HEK293-based knock-out cellular model devoid of both isoforms (COX4i1/2 KO). Subsequent knock-in of COX4i1 or COX4i2 generated cells with exclusive expression of respective isoform. Both isoforms complemented the respiratory defect of COX4i1/2 KO. The content, composition, and incorporation of COX into supercomplexes were comparable in COX4i1- and COX4i2-expressing cells. Also, COX activity, cytochrome c affinity, and respiratory rates were undistinguishable in cells expressing either isoform. Analysis of energy metabolism and the redox state in intact cells uncovered modestly increased preference for mitochondrial ATP production, consistent with the increased NADH pool oxidation and lower ROS in COX4i2-expressing cells in normoxia. Most remarkable changes were uncovered in COX oxygen kinetics. The p50 (partial pressure of oxygen at half-maximal respiration) was increased twofold in COX4i2 versus COX4i1 cells, indicating decreased oxygen affinity of the COX4i2-containing enzyme. Our finding supports the key role of the COX4i2-containing enzyme in hypoxia-sensing pathways of energy metabolism.