Study of the antioxidant activity of Pistacia atlantica Desf. Gall extracts and evaluation of the responsible compounds

The aim of this work is to evaluate the antioxidant effects of the extracts of Pistacia atlantica collected in the Khenifra region (Morocco) in 2016. Different methods were used to study these extract: 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity, the ferric reducing ability of plasma (FRAP) assay, the phosphomolybdate method for determining the total antioxidant capacity, and the electrochemical method for cyclic voltammetry were employed to evaluate the antioxidant capacity of Pistacia atlantica Desf. Phytochemical screening helped us to highlight the presence of secondary metabolites. The extraction of the phenolic compounds was carried out by the Soxhlet method in the presence of different mixtures solvents (ethanol/water and methanol/water); the fractions of the different extracts were affected using ethyl acetate and n-butanol. The dosage results showed that the ethanolic extract was rich in total phenols (260.4 mg GAE/g of the extract) and in total flavonoids (129.15 mg QE/g of the extract), while the butanolic fraction was rich in condensed tannin (50.96 mg CE/g of the extract). The qualitative analysis was performed by high performance liquid chromatography (HPLC). The main compounds that were identified in the methanolic and ethanolic extracts of Pistacia atlantica Desf were ascorbic acid, gallic acid, tannic acid, rutin, and quercetin. The results of the antioxidant activity revealed that the butanolic and ethyl acetate fractions exhibit a good iron reduction capacity (concentration that gave half maximal response, EC50 = 0.02 mg/ml and 0.03 mg/ml, respectively) and a very interesting antiradical activity with an IC50 (concentration of inhibitor where the response is reduced by half) = 0.08 mg/ml and 0.04 mg/ml, respectively. Cyclic voltammetry presented a single oxidation peak between 400 and 500 mV. The ethanolic and methanolic extracts were recorded from the oxidation currents values of 15.75 and 10.41 i/μA cm.2 respectively at the concentration 0.1 mg/ml. Hence, it is clear that the leaves of Pistacia atlantica Desf, which are currently often considered as potential antioxidants, contain antioxidants that can usefully be extracted and added to foods.

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Effects of Pistacia atlantica (subsp. Mutica) oil extracts on antioxidant activities during experimentally induced cutaneous wound healing in rats

Veterinary Science Development ◽

10.4081/vsd.2015.5672 ◽

2015 ◽

Vol 5 (1) ◽

Cited By ~ 2

Author(s):

Ahmad Reza Hamidi ◽

Aboutorab Tabatabaii Naeini ◽

Nader Tanideh ◽

Saeed Nazifi

Keyword(s):

Oxidative Stress ◽

Antioxidant Activity ◽

Wound Healing ◽

Antioxidant Enzymes ◽

Antioxidant Activities ◽

Thoracic Vertebrae ◽

Skin Defect ◽

Enzymatic Antioxidants ◽

Post Injury ◽

Pistacia Atlantica

The fruits of Pistacia atlantica (subsp. mutica) have been used traditionally for the treatment of peptic ulcer, as a mouth freshener and have recently been introduced as a source of antioxidant vegetable oils. The aim of this study was to investigate the antioxidant activity of the gel forms, from P. atlantica (subsp. mutica) oil extraction on enzymatic antioxidants in experimental wound created in rat. A square-shaped skin defect (2×2 cm) was created aseptically by surgical excision at the first thoracic vertebrae. Then animals were randomly allocated in four groups (I, untreated controls; II, topically treated base gel; III, topically treated 5% gel; IV, topically treated 10% gel). Blood sampling was accomplished at 3, 7, 10, 14 and 21 days post-injury. Samples were collected for measuring antioxidant enzymes activities (superoxide dismutase, catalase and glutathione peroxidase activity in red cells) and lipid peroxidation (plasma malondialdehyde). The data analysis generally evidenced that the activities of the main antioxidant enzymes began to decrease significantly at 7 days after the wound was created in control and base gel groups. This remarkable decline became more evident in the period between 10 to 21 days post injury but increased progressively in P. atlantica (subsp. mutica) treatment groups, especially in gel 10% treatment group during wound healing. The results of this study suggest that excision of the wound leads to oxidative stress and topical administration of P. atlantica (subsp. mutica) gels causes remarkable changes in antioxidant parameter during wound closure (especially gel 10%) via pro-oxidative, and antioxidant activity can improve oxidative stress.

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Antioxidant activity and phenolic profile of different organs of Pistacia atlantica Desf. subsp. atlantica from Algeria

Natural Product Research ◽

10.1080/14786419.2016.1217205 ◽

2016 ◽

Vol 31 (6) ◽

pp. 718-723 ◽

Cited By ~ 10

Author(s):

Fethi Toul ◽

Nabila Belyagoubi-Benhammou ◽

Amel Zitouni ◽

Fawzia Atik-Bekkara

Keyword(s):

Antioxidant Activity ◽

Phenolic Profile ◽

Pistacia Atlantica

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Pistacia atlantica Desf. roots extract: LC-ESI-MS Analysis, antioxidant activity and gastroprotective effect on experimentally-induced ultrastructural gastric ulcers in mice

Ultrastructural Pathology ◽

10.1080/01913123.2021.1896612 ◽

2021 ◽

pp. 1-10

Author(s):

Marwa Ben Hmed ◽

Hichem Alimi ◽

Fatma Guesmi ◽

Feriel Elatrech ◽

Nacim Zouari ◽

...

Keyword(s):

Antioxidant Activity ◽

Gastric Ulcers ◽

Pistacia Atlantica ◽

Esi Ms ◽

Gastroprotective Effect ◽

Ms Analysis ◽

Experimentally Induced

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Identification of phenolic compounds in the buds of Algerian Pistacia atlantica desf. Subsp. atlantica by antioxidant activity guided fractionation

Journal of Complementary and Integrative Medicine ◽

10.1515/jcim-2021-0336 ◽

2021 ◽

Vol 0 (0) ◽

Author(s):

Fethi Toul ◽

Sonia Moussouni ◽

Nacera Ghembaza ◽

Amel Zitouni ◽

Amina Djendar ◽

...

Keyword(s):

Antioxidant Activity ◽

Phenolic Compounds ◽

Spectroscopic Techniques ◽

Active Compounds ◽

Pistacia Atlantica ◽

Tlc Plates ◽

Dpph Scavenging

Abstract Objectives A bio-guided selection and identification of the most active compounds obtained from Algerian Pistacia atlantica desf. Subsp. atlantica. Methods An antioxidant activity guided fractionation was performed on buds’ extract using extensive chromatographic and spectroscopic techniques. The antioxidant potentials of isolated compounds and other unpurified fractions were evaluated against DPPH radicals using TLC plates and test tubes. Results The results showed that all isolated compounds and fractions exhibited eminent DPPH scavenging potential. Two coumarins (7-ethoxycoumarin and 7-hydroxy-5-methoxycoumarin) and two flavonoids (3′,5,7-Trihydroxy-4′-methoxyflavanone and 5,6,7,4′-tetrahydroxyflavonol-3-O-rutinoside) were isolated for the first times from the titled subspecies. Conclusions These results confirm that the species of P. atlantica is far from being exhausted of active compounds, especially polyphenols.

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Antioxidant activity of Pistacia atlantica var mutica kernel oil and it’s unsaponifiable matters

Journal of Food Science and Technology ◽

10.1007/s13197-019-04004-0 ◽

2019 ◽

Vol 56 (12) ◽

pp. 5336-5345 ◽

Cited By ~ 3

Author(s):

Javad Tavakoli ◽

Khodadad Hajpour Soq ◽

Alireza Yousefi ◽

Parviz Estakhr ◽

Mohsen Dalvi ◽

...

Keyword(s):

Antioxidant Activity ◽

Pistacia Atlantica ◽

Kernel Oil

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Total Tocopherols, Carotenoids, and Fatty Acids Content Variation of Pistacia atlantica from Different Organs’ Crude Oils and Their Antioxidant Activity during Development Stages

Chemistry & Biodiversity ◽

10.1002/cbdv.202000117 ◽

2020 ◽

Cited By ~ 2

Author(s):

Manel Chelghoum ◽

Hamid Guenane ◽

Mohamed Harrat ◽

Mohamed Yousfi

Keyword(s):

Fatty Acids ◽

Antioxidant Activity ◽

Crude Oils ◽

Pistacia Atlantica ◽

Development Stages ◽

Content Variation

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Harvest date and variability in lipid bioactive compounds in Pistacia atlantica

Mediterranean Journal of Nutrition and Metabolism ◽

10.3233/mnm-200511 ◽

2021 ◽

pp. 1-18

Author(s):

Mebarka Imane Benguechoua ◽

Madjda Benguechoua ◽

Nadhir Gourine ◽

Artur M. S. Silva ◽

Mokhtar Saidi ◽

...

Keyword(s):

Fatty Acids ◽

Antioxidant Activity ◽

Large Scale ◽

Unsaturated Fatty Acids ◽

Antioxidant Activities ◽

Essential Fatty Acids ◽

Saturated Fatty Acids ◽

Growth Period ◽

Bioactive Molecules ◽

Pistacia Atlantica

BACKGROUND: The present work stands as an endeavor to uncover the ideal harvesting time of leaves in which they exhibits the maximum contents of bioactive molecules such as essential fatty acids, tocopherols and carotenoids. METHODS: A large scale investigation was carried out for the leaves of Pistacia atlantica involving a large number of populations collected over a period of four months during the growing season. The antioxidant activity was evaluated using both DPPH and β-carotene assays. The chemical percentage variability of the fatty acids was investigated using statistical analysis methods (Agglomerative Hierarchical Clustering “AHC”, also cited as CAH). OBJECTIVE: During the growth period, the effects of harvesting date on the total contents of lipids, tocopherols, carotenoids, fatty acids composition and also the antioxidant activities of the lipids were investigated. RESULTS: The content of myristic acid and other saturated fatty acids increased during leaf development, while linoleic, linolenic acids and unsaturated fatty acids decreased. The highest percentages of both linolenic C18:3 (27.25±5.92%) and linoleic acids C18:2 (17.68±3.80%) were obtained for the month of May at the first stage of leaves development (young leaves), but higher percentage levels of C18:1, were obtained for both consecutive months of August & September (28.83±6.50%; 27.79±8.63%, respectively) at intermediate developing stage. The lipids, tocopherols, carotenoids contents and the saturated and unsaturated fatty acids were dependent on the harvest time. The antioxidant activity showed higher powers at the first developing stage (May). Two main clusters and two sub-clusters of the fatty acids were distinguished and were also depending clearly on the period of the collection. CONCLUSIONS: The results showed that the FAs were dependent on the period of collection of the leaves. The main result of this study illustrate the nutritional potential (richness in MUFA such as C18:1, 2, 3) of the oil of P. atlantica leaves, which can provide opportunities for rational exploitation in the food industries or for medicinal purposes.

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