Antitumor Efficacy of Silver Nanoparticles Biosynthe-sized from Marine Red Seaweed Halymenia porphyroides Boergesen on Dalton’s Lymphoma Ascites in Mice

The biosynthesized silver nanoparticles from marine red seaweed Halymenia porphyroides against Dalton’s lymphoma ascites (DLA)-induced tumor inoculation was studied for antitumor activity. The biosynthesized silver nanoparticles from marine red seaweed Halymenia porphyroides were given orally to Swiss albino mice (50 mg/kg/day) for 14 days showed a significant reduction in body weight, packed cell volume, andviable tumor cell count when compared to the mice of the DLA control group.The haematological parameters of the treatment group with biosynthesized silver nanoparticles also exhibited increased haemoglobin, RBCs, Platelets, and decreased WBCs compared to the DLA control group of mice. Similarly, the biochemical parameters like total cholesterol, aspartate amino-transferase (AST), alanine aminotransferase (ALT), triglycerides (TL), and alkaline phosphatase (ALP) of the treatment control group with biosynthesized silver nanoparticles reversed the parameters to normal levels compared to the DLA control group of mice. The antitumor efficacy of the biosynthesized silver nanoparticles from Halymenia porphyroides was confirmed based on the haematological, biochemical, life span, packed cell volume, cell count, and histopathological analysis.

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THE BLOOD PICTURE OF SHEEP FED ON FRESH AND DRIED MARROW-STEM KALE

Canadian Journal of Animal Science ◽

10.4141/cjas73-037 ◽

1973 ◽

Vol 53 (2) ◽

pp. 229-236 ◽

Cited By ~ 1

Author(s):

G. PELLETIER ◽

L. J. MARTIN

Keyword(s):

Low Temperature ◽

Cell Count ◽

Cell Volume ◽

Packed Cell Volume ◽

Red Cell ◽

Blood Cell Count ◽

Blood Picture ◽

Short Period ◽

Red Blood Cell Count ◽

Red Cell Count

Sheep were used to compare the anemia effects of fresh marrow-stem kale (Brassica oleracea var. acephala D.C.) with low-temperature dehydrated marrow-stem kale (MSK). There was a drop in hemoglobin (P < 0.01), packed-cell volume (P < 0.05), and red blood cell count (P < 0.05) after the 4th, 5th, and 6th wk, respectively, of feeding fresh MSK. Similar drops in hemoglobin (P < 0.05), packed-cell volume (P < 0.05), and red cell count (P < 0.05) occurred after the 4th wk of feeding dried MSK. Formation of Heinz-Ehrlich bodies peaked at the corresponding lowest values of hemoglobin and packed-cell volume for both fresh and dried MSK. These criteria returned almost to normal in the 2nd and 3rd wk after stopping the feeding of kale and using ground hay as the entire ration instead. It was concluded that high levels of either fresh or dried MSK could not be fed to sheep even for a relatively short period of time.

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Effect of ad libitum intake of an electrolyte repository in horses that underwent a polo game

Semina Ciências Agrárias ◽

10.5433/1679-0359.2018v39n1p187 ◽

2018 ◽

Vol 39 (1) ◽

pp. 187

Author(s):

Lorena Chaves Monteiro ◽

José Dantas Ribeiro Filho ◽

Waleska De Melo Ferreira Dantas ◽

Bruna Maria Ribeiro ◽

Micheline Ozana da Silva ◽

...

Keyword(s):

Cell Volume ◽

Packed Cell Volume ◽

Chloride Concentration ◽

Control Group ◽

Significance Level ◽

Group Water ◽

Calcium Magnesium ◽

Ad Libitum ◽

Ad Libitum Intake ◽

And Control

The objective of this study was to assess the effects of an electrolyte and energy repository on the hematologic and biochemical variables of horses after a polo game and compare them to the effects of a commercial electrolyte repository and water. Twelve healthy horses, aged 5 to 10 years, mean body weight 356.25 ± 25.6 kg and in training for polo games, were randomly divided into three groups of four animals each and received one of three treatments. Animals participated in a 7-minute polo game. At the end of the match, they were taken to individual stalls where they received treatments for spontaneous hydration for 6 hours. Treatments were Hydroelectrolytic and Energy Repository (RHE) containing sodium, chloride, potassium, calcium, magnesium, dextrose, maltodextrin and, sucrose; and Commercial Paste (PCO) containing calcium, fructooligosaccharides, glycine, magnesium, potassium, sodium, and ad libitum water. The control group (Water) received water. Blood samples were collected as follows: just before the beginning of exercise (T0), immediately after exercise (T1), 3 hours after the end of exercise (T3), and 6 hours after the end of exercise (T6). The volume of voluntary intake was measured at T3 and T6. Packed cell volume and serum concentrations of sodium, potassium, chloride, calcium, magnesium, phosphorus, creatinine, total protein, plasma glucose, and lactate were measured. Data were submitted to descriptive statistics (mean ± standard deviation), Lilliefors and Cochran & Bartlett tests, analysis of variance (ANOVA), and Tukey or Duncan tests at a 5% significance level. The net volume ingested by the RHE group was higher than the PCO and control groups. In all groups, a reduction in chloride concentration and increases in packed cell volume, protein, creatinine, glucose, and lactate concentrations were observed in T1. These variables returned to the values found at T0 throughout the rehydration phase (T3 and T6). It is concluded that the ingestion of the hydroelectrolytic energy repository does not cause alteration in the biochemical profile of the animals.

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EFFECTS OF PROBIOTIC SACCHAROMYCES BOULARDII AND CO-TRIMOXAZOLE ON SOME HAEMATOLOGICAL PARAMETERS IN ADULT WISTAR RATS

African Journal of Health, Safety and Environment ◽

10.52417/ajhse.v2i2.158 ◽

2021 ◽

Vol 2 (2) ◽

pp. 55-62

Author(s):

P. A. Obazelu ◽

I. A. Aruomaren ◽

C. C. Nwangwu

Keyword(s):

Body Weight ◽

Blood Cell ◽

Cell Count ◽

Cell Volume ◽

White Blood Cell Count ◽

White Blood Cell ◽

Packed Cell Volume ◽

Saccharomyces Boulardii ◽

Total White Blood Cell ◽

Blood Cell Count

Saccharomyces boulardii is a probiotic yeast used as a nutritional supplement. Co-trimoxazole, is a broad-spectrum antimicrobial agent used in the prophylaxis and treatment of infections.The goal of this study was to evaluate the individual and interactive effects of probiotic Saccharomyces boulardii and co-trimoxazole on some haematological parameters in albino rats. Thirty (30) albino Wistar rats weighing 150-200g were divided into five groups; Basal, A, B, C, and D, with six animals in each group. Group A (control) was given a normal rat pellet (standardized feed) and clean water (pH 6.5- 8.5). Group B was administered 5.5mg/kg body weight of the commercial probiotics (Saccharomyces boulardii). Group C was administered 4.4mg/kg body weight of co-trimoxazole. Group D was administered 5.5mg/kg body weight of the commercial probiotics and 4.4mg/kg body weight of co-trimoxazole. All administrations were done using a gavage tube for 15 days. Blood samples were collected for analysis on days 0, 5 and 15. The results showed that Saccharomyces boulardii caused a significant increase (P<0.05) in total white blood cell count and packed cell volume of the treated groups at day 5. On day 15, the total white blood cell count (10.67±0.66), packed cell volume (43.23±0.47), platelet count (932±38.89) and mean platelet volume (8.53±0.38) showed a significant increase (P<0.05) in the probiotic group. The co-trimoxazole group showed a significant reduction (P<0.05) in the packed cell volume and total white blood cell count. This study shows that Saccharomyces boulardii has a positive effect on the haematological profile of the test animals.

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Effect of pregnancy on packed cell volume and total white blood cells count among Sudanese pregnant women attending antenatal care at Ribat University Hospital (Khartoum state)

International Journal of Reproduction Contraception Obstetrics and Gynecology ◽

10.18203/2320-1770.ijrcog20180150 ◽

2018 ◽

Vol 7 (2) ◽

pp. 371

Author(s):

Wafa Mubarak M. Ahmed ◽

Abdurrahman Khalid ◽

O. A. Musa

Keyword(s):

Antenatal Care ◽

Pregnant Women ◽

Cell Volume ◽

Packed Cell Volume ◽

Blood Cells ◽

White Blood Cells ◽

University Hospital ◽

Control Group ◽

P Values ◽

Significant Difference

Background: During pregnancy the women undergo several structural and physiological changes almost in all body systems including the hematological profile. The main reported hematological change is the plasma expansion and haemodilution which affect the red blood cells(RBCs) and white blood cells (WBCs) count in addition to other hematological indices including packed cell volume (PCV). The objective of this study was to assess the effect of pregnancy on packed cell volume and white blood cells among Sudanese pregnant women and derive a formula for the normal values in pregnant women in relation to the packed cell volume.Methods: An analytical case control study was conducted in Khartoum state, Sudan 2017. The study included20 Sudanese pregnant women in the second and third trimesters attending the antenatal care at The National Ribat university hospital and 20 Sudanese non-pregnant women in the same age group as control. A questionnaire was used to collect socio-demographic and clinical data.5ml of venous blood was collected in a container containing EDTA. Complete blood count (CBC) was measured by using automated cell counter Sysmex.Results: There was statistically significant decrease in PCV value during 2nd and 3rd trimesters of pregnancy compared to the non-pregnant control group with p values (0.00 and 0.04 respectively), and statistically significant increase in WBCs count during 2nd and 3rd trimesters of pregnancy compared to the non-pregnant control group with p values (0.04 and 0.00 respectively). Also, a progressive increase in WBCs count with gestational age was demonstrated (second trimester 6.65×103/uL, third trimester 7.43×103/uL). The results showed mean WBCs of 7.79×103/uL, when compared to the estimated values the results showed no significant difference with the mean WBCs in 2ndand 3rdtrimesters (p value 0.06 and 0.39 respectively).Conclusions: During pregnancy there was statistically significant decrease in PCV values, statistically significant increase in WBCs count and by considering the haemodilution factor there was a higher increase in WBCs count.

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Assessment of White Blood Cell Count, Packed Cell Volume, Phagocytic Functions, Serum Albumin and Plasma Iron in HIV Infected Patients

Clinical Immunology ◽

10.1016/j.clim.2010.03.329 ◽

2010 ◽

Vol 135 ◽

pp. S110 ◽

Cited By ~ 2

Author(s):

Charles Onyenekwe ◽

Prince Ele ◽

Nkiruka Ukibe ◽

Michael Ezeani ◽

Clement Ezechukwu ◽

...

Keyword(s):

Blood Cell ◽

Serum Albumin ◽

Cell Count ◽

Cell Volume ◽

White Blood Cell Count ◽

White Blood Cell ◽

Packed Cell Volume ◽

Blood Cell Count ◽

Plasma Iron

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PSII-16 Effects of Oligosaccharide-based Polymer on Blood Profiles in Weanling Pigs Experimentally Infected with a Pathogenic E. coli

Journal of Animal Science ◽

10.1093/jas/skab054.282 ◽

2021 ◽

Vol 99 (Supplement_1) ◽

pp. 167-168

Author(s):

Kwangwook Kim ◽

Yijie He ◽

Cynthia Jinno ◽

Seijoo Yang ◽

Xunde Li ◽

...

Keyword(s):

Blood Cell ◽

Cell Count ◽

Cell Volume ◽

Packed Cell Volume ◽

Blood Cell Count ◽

E Coli ◽

Cell Counts ◽

Blood Cell Counts ◽

White Blood Cell Counts ◽

Blood Profiles

Abstract The objective of this experiment was to investigate dietary supplementation of oligosaccharide-based polymer on blood profiles of weaned pigs experimentally infected with a pathogenic F18 Escherichia coli (E. coli). Forty-eight pigs (7.23 ± 1.11 kg BW) were individually housed in disease containment rooms and randomly allotted to one of four treatments with 12 replicate pigs per treatment. The four dietary treatments were a nursery basal diet (control), and 3 additional diets supplemented with 50 mg/kg Mecadox (AGP), 10 or 20 mg/kg of oligosaccharide-based polymer. The experiment lasted 18 d [7 d before and 11 d after the first inoculation (d 0)]. The doses of F18 E. coli inoculum were 1010 cfu/3 mL oral dose daily for 3 days. Blood samples were collected before E. coli inoculation (d 0), and on d 2, 5, 8, and 11 post-inoculation (PI). Total and differential blood cell count were analyzed by CBC test. All data were analyzed by ANOVA using the PROC MIXED of SAS with pig as the experimental unit. Supplementation of oligosaccharide-based polymer linearly (P < 0.05) reduced white blood cell counts, neutrophils, eosinophils, and basophils on d 2 PI, and neutrophils on d 5 PI, compared with the control. No differences were observed in total and differential white blood cell counts among AGP and two oligosaccharide-based polymer treatments except that pigs fed with AGP had greater (P < 0.05) lymphocytes on d 2 PI compared with pigs fed with oligosaccharide-based polymer diets. Supplementation of low dose oligosaccharide-based polymer or AGP reduced (P < 0.05) red blood cell count and packed cell volume on d 2 PI, whereas inclusion of high dose oligosaccharide-based polymer or AGP reduced (P < 0.05) packed cell volume on d 5 PI, compared with the control. In conclusion, supplementation of oligosaccharide-based polymer may alleviate the systemic inflammation caused by F18 E. coli infection.

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Effect of Physical Exercise on some Hematological Parameters in Female Athletes in Bangladesh

Journal of Nepal Medical Association ◽

10.31729/jnma.2710 ◽

1970 ◽

Vol 52 (195) ◽

pp. 892-896 ◽

Cited By ~ 1

Author(s):

Tanbira Alam ◽

Lubna Shirin ◽

SM Niazur Rahman ◽

Tanjeda Alam ◽

Nasrin Habib ◽

...

Keyword(s):

Physical Exercise ◽

Cell Volume ◽

Packed Cell Volume ◽

Serum Iron ◽

Female Athletes ◽

Ferritin Level ◽

Hematological Parameters ◽

Control Group ◽

Group A ◽

Group B

Introduction: Long term physical training has been considered to adversely affect the performance of athletes especially the females. It may be due to the iron depletion caused by hemolysis or hemodilution results from plasma volume expansion. This study aims to assess the effect of heavy exercise on hemoglobin concentration and some other hematological parameters in female athletes. Methods: This cross sectional study was carried out in the Department of physiology, Dhaka Medical College, Dhaka, from July 2010-June 2011. A total number of 105 females (70 athletes and 35 non athletes) between 15-25 years of age were recruited. The 35 apparently healthy female non athletes were taken as control (Group-A) and 70 female athletes comprise the study group (Group-B). According to sporting category, Group-B was further subdivided as B1:Runners and B2:Cyclists. Hemoglobin percentages (Hb %), red blood cell (RBC) concentration, packed cell volume (PCV), serum iron, serum ferritin and total iron binding capacity (TIBC) were measured in fasting state after two months training. Statistical analysis was done by using SPSS/ win.15.0 for comparison between the groups, using student’s unpaired “t” test. Results: Mean Hb%, RBC, PCV, iron and ferritin level were significantly (P<0.001) lower in athletes than those of control group. Within the study groups, runner’s serum iron and ferritin level were lower than cyclists. TIBC significantly (P<0.001) rose in Group-B2 in comparison to Group-A. Conclusions: The current study showed that intense physical exercise leads to early stages of Hb%, RBC, PCV, iron and ferritin depletion which might compromise the health and performance of athletes. Keywords: exercise; hemoglobin; packed cell volume; serum iron.

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ANTI-ANAEMIC POTENTIAL OF METHANOLIC LEAF EXTRACT OF MUCUNA PRURIENS ON PHENYLHYDRAZINE (PHZ) INDUCED ANAEMIC ALBINO WISTAR RATS

FUDMA Journal of Sciences ◽

10.33003/fjs-2020-0403-399 ◽

2020 ◽

Vol 4 (3) ◽

pp. 370-374

Author(s):

Kasang Naman ◽

Habibat Oseni ◽

Emmanuel Enoh

Keyword(s):

Vitamin B12 ◽

Cell Volume ◽

Normal Control ◽

Normal Saline ◽

Packed Cell Volume ◽

Wistar Rats ◽

Leaf Extract ◽

Lethal Dose ◽

Mucuna Pruriens ◽

Control Group

The antianaemic potential of methanolic leaf extracts of Mucuna pruriens was investigated using phenylhydrazine (PHZ) induced anaemic albino Wistar rats. Fifteen rats used for the study were randomized into five experimental groups. To induced anaemia, the rats (except the normal control, Group E), received 60 mg/kg of the haemolytic agent Phenylhydrazine intraperitoneally (i.p) for two consecutive days. Anaemic Wistar rats in groups A and B received a daily oral dose of 500 and 250 mg/kg of the methanolic leaf extract of Mucuna pruriens. Nweze et al. (2016) had reported a median lethal dose greater than 5000 mg/kg for the methanol leaf extract of Mucuna pruriens. Groups C and D received Vitamin B12 (10 mg/kg) and normal saline (1 ml/kg), respectively. Normal control rats also received normal saline (1 ml/kg). Extract or normal saline was administered per os (p.o) while vitamin B12 was administered i.p. for a duration of 21 days. Packed cell volume (PCV) and haemoglobin concentration were determined weekly for three weeks. The result of the study indicated that both the methanolic leaf extract of Mucuna pruriens and Vitamin B12 significantly (p < 0.05) increased the packed cell volume and haemoglobin concentrations in treated rats compared to the negative control group of rats. This indicated that the methanolic leaf extract of Mucuna pruriens has anti-anaemic properties and could be utilized in the management of anaemia

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Haematological and Genotoxic effects of cadmium chloride on Mesopotamichthys sharpeyi

Baghdad Science Journal ◽

10.21123/bsj.13.4.745-752 ◽

2016 ◽

Vol 13 (4) ◽

pp. 745-752

Author(s):

Baghdad Science Journal

Keyword(s):

Red Blood Cells ◽

Cell Volume ◽

Packed Cell Volume ◽

Blood Cells ◽

Cadmium Chloride ◽

White Blood Cells ◽

Hemoglobin Concentration ◽

Control Group ◽

Blood Picture ◽

Genotoxic Effects

The present study investigated Haematological changes in Mesopotamichthys sharpeyi, as well as determination genotoxic effects of cadmium chloride on bunni fish by using 120 fingerlings, fish were distributed randomly into four treatments in addition to control group. Fish in first group treated (T1) with cadmium 0.093mg/L with changing water and added cadmium continuously, fish in the second group treated (T2) with cadmium 0.093mg/L with changing water without adding cadmium, third treatment (T3) with cadmium 0.046mg/L with changing water and adding cadmium continuously, and fourth treatment (T4) with cadmium 0.046mg/L with changing water without adding cadmium. Results of blood picture in T1 and T3 showed a significant reduction in red blood cells count, hemoglobin concentration, packed cell volume values, while the number of white blood cells showed a significant increase in its values. Results showed presence of improvement of clinical and microscopical signs and blood picture in T2 and T4, were changed water aquarium continuously and added cadmium only once compared withT1 and T3. Results of the present study concluded that changing water aquarium in the treatments without adding cadmium led to improvement of health status of fish which increased with the passage of time results of blood picture were almost the same of the control group. It could be concluded from the current study that the adding of cadmium to water aquarium containing bunni fish led to decrease in red blood cells count, hemoglobin and packed cell volume values and increase in micronuclei number.

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