Iminodisuccinic Acid Relieved Cadmium Stress in Rapeseed Leaf by Affecting Cadmium Distribution and Cadmium Chelation With Pectin

Rapeseed (Brassica napus. L) is a nutritious vegetable, while cadmium (Cd) pollution threatens the growth, productivity, and food security of rapeseed. By studying the effects of iminodisuccinic acid (IDS), an easily biodegradable and environmental friendly chelating agent, on Cd distribution at the organ and cellular level, we found IDS promoted dry matter accumulation of rapeseed and increased the contents of photosynthetic pigment in leaves. Inhibited root-shoot Cd transport resulted in higher activity of antioxidant enzymes and decreased hydrogen peroxide (H2O2) and malondialdehyde (MDA) accumulation in leaves, which indicated that IDS contributed to alleviating Cd-caused oxidative damage in leaf cells. Additionally, IDS increased Cd distribution in cell wall (CW) and relieved Cd toxicity in organelle of leaves, while IDS did not change the contents of different CW components. The improved Cd fixation in leaf CW was mainly attributed to enhanced demethylation of covalently bound pectin (CSP) and Cd chelation with CSP.

Strippable Polymeric Nanocomposites Comprising “Green” Chelates, for the Removal of Heavy Metals and Radionuclides

The issue of heavy metal and radionuclide contamination is still causing a great deal of concern worldwide for environmental protection and industrial sites remediation. Various techniques have been developed for surface decontamination aiming for high decontamination factors (DF) and minimal environmental impact, but strippable polymeric nanocomposite coatings are some of the best candidates in this area. In this study, novel strippable coatings for heavy metal and radionuclides decontamination were developed based on the film-forming ability of polyvinyl alcohol, with the remarkable metal retention capacity of bentonite nanoclay, together with the chelating ability of sodium alginate and with “new-generation” “green” complexing agents: iminodisuccinic acid (IDS) and 2-phosphonobutane-1,2,4-tricarboxylic acid (PBTC). These environmentally friendly water-based decontamination solutions are capable of generating strippable polymeric films with optimized mechanical and thermal properties while exhibiting high decontamination efficiency (DF ≈ 95–98% for heavy metals tested on glass surface and DF ≈ 91–97% for radionuclides 241Am, 90Sr-Y and 137Cs on metal, painted metal, plastic, and glass surfaces).

EFFECT OF COMPLEXONS, DERIVATIVES OF SUCCINIC ACID, ON THE FORMATION OF CAROTENOIDS IN GREEN PLANTS

На основании обобщения результатов многолетних лабораторных и полевых опытов обнаружена зависимость уровня содержания жёлтых фотосинтетических пигментов в растениях от внекорневой обработки растворами комплексонов. Комплексоны, производные янтарной кислоты (иминодиянтарная кислота, этилендиаминдиянтарная кислота, диаминоциклогександиянтарная кислота) способствуют накоплению каротиноидов в зелёных растениях, а этилендиаминтетрауксусная кислота существенно не влияет на их содержание. Based on the generalization of the results of many years of laboratory and field experiments, the dependence of the content of yellow photosynthetic pigments in plants on foliar treatment with solutions of complexones was found. Complexones, derivatives of succinic acid (iminodisuccinic acid, ethylenediaminedisuccinic acid, diaminocyclohexanedisuccinic acid) contribute to the accumulation of carotenoids in green plants, and ethylenediaminetetraacetic acid does not significantly affect their content.

The Study of Degradation of Complexones, Derivatives of Succinic Acid, and their Boron-Containing Complexes Under the Influence of Microbiological Preparations

The results of the study of the possibility of degradation of complexones, derivatives of succinic acid (CDSA), iminodisuccinic acid (IDSA), ethylenediaminedisuccinic acid (EDCSA), trans-1, 2-diaminocyclohexanedisuccinic acid (DCHDSA) and complexones taken for comparison, derivatives of iminodiacetic acid (IDSA) and ethylenediaminetetraacetic acid (EDTAA) under the influence of microbiological preparations “Baikal EM-1” and “Rhizoplane L”, as well as borate complexes of CDSA: B-IDSA, B-EDCSA and B-DCHDSA under the influence of the preparation Rhizoplane L at a temperature 22±1oC in an aqueous medium. It was found that within 4.5 months of the compounds taken for research under the action of Rhizoplane L preparation, B-IDSA was degraded to greatest extent (by 88%), and under the action of Baikal EM-1 preparation more than other degraded CDSA (by 86%) and EDTAA. Simultaneous destruction of DCHDSA and B-DCHDSA when exposed to the preparation taken for the experiment was not noted.

COBALT COMPLEXONES BASED ON ENVIRONMENTALLY FRIENDLY COMPLEXONS AS MICRO-ELEMENT FERTILIZERS

Представлены результаты эксперимента по применению созданных хелатных ростостимуляторов на основе экологически безопасных комплексонов для отдельных классов растений. В условиях мелкоделяночного полевого опыта исследовано влияние иминодиянтарной кислоты (ИДЯК), этилендиаминтетрауксусной кислоты (ЭДТУК), хелатных соединений кобальта (II) на их основе: Со-ИДЯК и Со-ЭДТУК, соответственно, на растения фасоли обыкновенной. Обнаружено увеличение содержания хлорофилла и общей массы семян под воздействием ИДЯК и Со-ИДЯК. Из использованных кобальтовых микроудобрений наиболее эффективным оказался комплекс Со-ИДЯК. The results of an experiment on the use of created chelated growth stimulants based on environmentally friendly complexones for certain classes of plants are presented. Under the conditions of a small-plot field experiment, the effect of iminodisuccinic acid (IDS), ethylenediaminetetraacetic acid (EDTA), and chelate compounds of cobalt (II) based on them: Co-IDS and Co-EDTA, respectively, on common bean plants was studied. An increase in the content of chlorophyll and the total mass of seeds was found under the influence of IDS and Co-IDS. Of the cobalt micronutrient fertilizers used, the Co-IDS complex turned out to be the most effective.

Study on the affinity characteristics of proteins on the immobilized metal affinity chromatography column

Study on affinity characteristic paramaters (KPML & ΛPML) of proteins on the iminodisuccinic acid metal chelate copper column by frontal chromatography.