Platelet Lysate for Mesenchymal Stromal Cell Culture in the Canine and Equine Species: Analogous but Not the Same

Platelet lysate (PL) is an attractive platelet-based therapeutic tool and has shown promise as xeno-free replacement for fetal bovine serum (FBS) in human and equine mesenchymal stromal cell (MSC) culture. Here, we established a scalable buffy-coat-based protocol for canine PL (cPL) production (n = 12). The cPL was tested in canine adipose MSC (n = 5) culture compared to FBS. For further comparison, equine adipose MSC (n = 5) were cultured with analogous equine PL (ePL) or FBS. During canine blood processing, platelet and transforming growth factor-β1 concentrations increased (p < 0.05 and p < 0.001), while white blood cell concentrations decreased (p < 0.05). However, while equine MSC showed good results when cultured with 10% ePL, canine MSC cultured with 2.5% or 10% cPL changed their morphology and showed decreased metabolic activity (p < 0.05). Apoptosis and necrosis in canine MSC were increased with 2.5% cPL (p < 0.05). Surprisingly, passage 5 canine MSC showed less genetic aberrations after culture with 10% cPL than with FBS. Our data reveal that using analogous canine and equine biologicals does not entail the same results. The buffy-coat-based cPL was not adequate for canine MSC culture, but may still be useful for therapeutic applications.

Ag–Au Core–Shell Triangular Nanoprisms for Improving p-g-C3N4 Photocatalytic Hydrogen Production

Ag–Au core–shell triangular nanoprisms (Ag@Au TNPs) have aroused extensive research interest in the field of hydrogen evolution reaction (HER) due to their strong plasmon effect and stability. Here, Ag@Au TNPs were fabricated by the galvanic-free replacement method. Then, we loaded them on protonated g-C3N4 nanoprisms (P–CN) by the electrostatic self-assembly method as an efficient plasmonic photocatalyst for HER. The hydrogen production rate of Ag@Au TNPs/P–CN (4.52 mmol/g/h) is 4.1 times higher than that of P–CN (1.11 mmol/g/h) under simulated sunlight irradiation, making it the most competitive material for water splitting. The formed Schottky junction helps to trap the hot electrons generated from Ag@Au TNPs, and the well-preserved tips of the Ag@Au TNPs can effectively generate an electromagnetic field to inhibit the photogenerated electron–holes pairs recombination. This study suggests that the rational design of Ag@Au TNPs by the galvanic-free replacement method is an effective co-catalyst for HER and boosting the additional combination of plasmonic metals and catalyst metals for the enhancement to HER.

Imperfect Repair Strategies for Two-Dimensional Warranty

<p>When a repairable product under warranty fails, the manufacturer (warrantor) has the choice to either repair or replace the failed product. When repairing a failed product, the degree of repair which affects the working condition of the product can vary, and this is assumed to have an impact on the cost of the repair. The main motivation of this study is to develop a warranty repair strategy that minimizes the costs associated with servicing the warranty. In this research, the product coverage is represented by a two-dimensional rectangular region with a free-replacement warranty. We propose an imperfect repair strategy that suggests employing imperfect repairs of a predefined degree, in prespecified subregions of the warranty region. The aim is to then minimize the expected warranty servicing cost to the manufacturer by determining the optimal partitioning of the warranty region for the chosen degrees of repair. Two imperfect repair models are considered, and for both, the expressions for the distribution of the times to imperfect repair and the expected warranty servicing cost per product sold are derived. We numerically illustrate our findings and compare the expected costs of the proposed imperfect repair strategy with those of previously developed repair-replacement warranty strategies.</p>

Imperfect Repair Strategies for Two-Dimensional Warranty

<p>When a repairable product under warranty fails, the manufacturer (warrantor) has the choice to either repair or replace the failed product. When repairing a failed product, the degree of repair which affects the working condition of the product can vary, and this is assumed to have an impact on the cost of the repair. The main motivation of this study is to develop a warranty repair strategy that minimizes the costs associated with servicing the warranty. In this research, the product coverage is represented by a two-dimensional rectangular region with a free-replacement warranty. We propose an imperfect repair strategy that suggests employing imperfect repairs of a predefined degree, in prespecified subregions of the warranty region. The aim is to then minimize the expected warranty servicing cost to the manufacturer by determining the optimal partitioning of the warranty region for the chosen degrees of repair. Two imperfect repair models are considered, and for both, the expressions for the distribution of the times to imperfect repair and the expected warranty servicing cost per product sold are derived. We numerically illustrate our findings and compare the expected costs of the proposed imperfect repair strategy with those of previously developed repair-replacement warranty strategies.</p>

Imperfect Repair Strategies for Two-Dimensional Warranty

<p>When a repairable product under warranty fails, the manufacturer (warrantor) has the choice to either repair or replace the failed product. When repairing a failed product, the degree of repair which affects the working condition of the product can vary, and this is assumed to have an impact on the cost of the repair. The main motivation of this study is to develop a warranty repair strategy that minimizes the costs associated with servicing the warranty. In this research, the product coverage is represented by a two-dimensional rectangular region with a free-replacement warranty. We propose an imperfect repair strategy that suggests employing imperfect repairs of a predefined degree, in prespecified subregions of the warranty region. The aim is to then minimize the expected warranty servicing cost to the manufacturer by determining the optimal partitioning of the warranty region for the chosen degrees of repair. Two imperfect repair models are considered, and for both, the expressions for the distribution of the times to imperfect repair and the expected warranty servicing cost per product sold are derived. We numerically illustrate our findings and compare the expected costs of the proposed imperfect repair strategy with those of previously developed repair-replacement warranty strategies.</p>

Hydrophobically Modified Isosorbide Dimethacrylates as a Bisphenol-A (BPA)-Free Dental Filling Material

A series of bio-based hydrophobically modified isosorbide dimethacrylates, with para-, meta-, and ortho- benzoate aromatic spacers (ISBGBMA), are synthesized, characterized, and evaluated as potential dental restorative resins. The new monomers, isosorbide 2,5-bis(4-glyceryloxybenzoate) dimethacrylate (ISB4GBMA), isosorbide 2,5-bis(3-glyceryloxybenzoate) dimethacrylate (ISB3GBMA), and isosorbide 2,5-bis(2-glyceryloxybenzoate) dimethacrylate (ISB2GBMA), are mixed with triethylene glycol dimethacrylate (TEGDMA) and photopolymerized. The resulting polymers are evaluated for the degree of monomeric conversion, polymerization shrinkage, water sorption, glass transition temperature, and flexural strength. Isosorbide glycerolate dimethacrylate (ISDGMA) is synthesized, and Bisphenol A glycerolate dimethacrylate (BisGMA) is prepared, and both are evaluated as a reference. Poly(ISBGBMA/TEGDMA) series shows lower water sorption (39–44 µg/mm3) over Poly(ISDGMA/TEGDMA) (73 µg/mm3) but higher than Poly(BisGMA/TEGDMA) (26 µg/mm3). Flexural strength is higher for Poly(ISBGBMA/TEGDMA) series (37–45 MPa) over Poly(ISDGMA/TEGDMA) (10 MPa) and less than Poly(BisGMA/TEGDMA) (53 MPa) after immersion in phosphate-buffered saline (DPBS) for 24 h. Poly(ISB2GBMA/TEGDMA) has the highest glass transition temperature at 85 °C, and its monomeric mixture has the lowest viscosity at 0.62 Pa·s, among the (ISBGBMA/TEGDMA) polymers and monomer mixtures. Collectively, this data suggests that the ortho ISBGBMA monomer is a potential bio-based, BPA-free replacement for BisGMA, and could be the focus for future study.

Fundamentals of tin iodide perovskites: a promising route to highly efficient, lead-free solar cells

Hybrid tin-iodide perovskites are investigated as potential lead-free replacement of the lead-iodide perovskites; however, the intrinsic operational limit of these systems has not been described in detail, so far. In...